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P99075

- ALF2_STAAN

UniProt

P99075 - ALF2_STAAN

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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Glyceraldehyde 3-phosphateBy similarity
Active sitei85 – 851Proton donorBy similarity
Metal bindingi86 – 861Zinc 1; catalyticBy similarity
Metal bindingi107 – 1071Zinc 2By similarity
Metal bindingi137 – 1371Zinc 2By similarity
Metal bindingi181 – 1811Zinc 1; catalyticBy similarity
Binding sitei182 – 1821Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi209 – 2091Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. fructose 1,6-bisphosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-2060-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Synonyms:fbaA
Ordered Locus Names:SA1927
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000751: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Fructose-bisphosphate aldolasePRO_0000178737Add
BLAST

2D gel databases

SWISS-2DPAGEP99075.

Interactioni

Protein-protein interaction databases

STRINGi158879.SA1927.

Structurei

3D structure databases

ProteinModelPortaliP99075.
SMRiP99075. Positions 1-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 2123Dihydroxyacetone phosphate bindingBy similarity
Regioni231 – 2344Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227793.
KOiK01624.
OMAiCTTRYEA.
OrthoDBiEOG6HXJ7B.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011289. Fruc_bis_ald_class-2.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEiPS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P99075-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLVSMKEML IDAKENGYAV GQYNINNLEF TQAILEASQE ENAPVILGVS
60 70 80 90 100
EGAARYMSGF YTIVKMVEGL MHDLNITIPV AIHLDHGSSF EKCKEAIDAG
110 120 130 140 150
FTSVMIDASH SPFEENVATT KKVVEYAHEK GVSVEAELGT VGGQEDDVVA
160 170 180 190 200
DGIIYADPKE CQELVEKTGI DALAPALGSV HGPYKGEPKL GFKEMEEIGL
210 220 230 240 250
STGLPLVLHG GTGIPTKDIQ KAIPFGTAKI NVNTENQIAS AKAVRDVLNN
260 270 280
DKEVYDPRKY LGPAREAIKE TVKGKIKEFG TSNRAK
Length:286
Mass (Da):30,836
Last modified:October 11, 2004 - v1
Checksum:i7A5FD0EBD48283D9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB43211.1.
PIRiB90006.
RefSeqiNP_375232.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB43211; BAB43211; BAB43211.
GeneIDi1124828.
KEGGisau:SA1927.
PATRICi19576312. VBIStaAur116463_2083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB43211.1 .
PIRi B90006.
RefSeqi NP_375232.1. NC_002745.2.

3D structure databases

ProteinModelPortali P99075.
SMRi P99075. Positions 1-285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 158879.SA1927.

2D gel databases

SWISS-2DPAGE P99075.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB43211 ; BAB43211 ; BAB43211 .
GeneIDi 1124828.
KEGGi sau:SA1927.
PATRICi 19576312. VBIStaAur116463_2083.

Phylogenomic databases

eggNOGi COG0191.
HOGENOMi HOG000227793.
KOi K01624.
OMAi CTTRYEA.
OrthoDBi EOG6HXJ7B.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .
BioCyci SAUR158879:GJCB-2060-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR011289. Fruc_bis_ald_class-2.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEi PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: N315.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: N315.
  3. "Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315."
    Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.
    Submitted (OCT-2007) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: N315.

Entry informationi

Entry nameiALF2_STAAN
AccessioniPrimary (citable) accession number: P99075
Secondary accession number(s): Q99SD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: October 1, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3