P99073 (OTC_STAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	SA1012

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000113012
Regions
	Region	56 – 60	5	Carbamoyl phosphate binding By similarity
	Region	272 – 275	4	Ornithine binding By similarity
Sites
	Binding site	107	1	Carbamoyl phosphate By similarity
	Binding site	134	1	Carbamoyl phosphate By similarity
	Site	31	1	Important for structural integrity By similarity
	Site	147	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P99073 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 1AEBBE5E81CAD27A	FASTA	333	37,535
	10 20 30 40 50 60 MKNLRNRSFL TLLDFSRQEV EFLLTLSEDL KRAKYIGTEK PMLKNKNIAL LFEKDSTRTR 70 80 90 100 110 120 CAFEVAAHDQ GANVTYLGPT GSQMGKKETT KDTARVLGGM YDGIEYRGFS QRTVETLAEY 130 140 150 160 170 180 SGVPVWNGLT DEDHPTQVLA DFLTAKEVLK KDYADINFTY VGDGRNNVAN ALMQGAAIMG 190 200 210 220 230 240 MNFHLVCPKE LNPTDELLNR CKNIAAENGG NILITDDIDQ GVKGSDVIYT DVWVSMGEPD 250 260 270 280 290 300 EVWKERLELL KPYQVNKEMM DKTGNPNVIF EHCLPSFHNA DTKIGQQIFE KYGIREMEVT 310 320 330 DEVFESKASV VFQEAENRMH TIKAVMVATL GEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: N315.
[2]	"Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth." Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y., Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J. J. Microbiol. Methods 60:247-257(2005) [PubMed: 15590099] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: N315.
[3]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: N315.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB42264.1.
PIR	D89888.
RefSeq	NP_374285.1. NC_002745.2.
3D structure databases
ProteinModelPortal	P99073.
SMR	P99073. Positions 2-333.
ModBase	Search...
Protein-protein interaction databases
STRING	P99073.
2D gel databases
SWISS-2DPAGE	P99073.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000000406; EBSTAP00000000406; EBSTAG00000000406.
GeneID	1123842.
GenomeReviews	Gene locus SA1012 in contig BA000018_GR.
KEGG	sau:SA1012.
PATRIC	19574236. VBIStaAur116463_1088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0078.
GeneTree	EBGT00050000023906.
HOGENOM	HBG579429.
OMA	DFRIFAP.
ProtClustDB	PRK04284.
Enzyme and pathway databases
BioCyc	SAUR158879:SA1012-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_STAAN

Accession

Primary (citable) accession number: P99073
Secondary accession number(s): Q9K3A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program