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Protein

Peroxiredoxin-5, mitochondrial

Gene

Prdx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Cysteine sulfenic acid (-SOH) intermediateSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-MMU-3299685. Detoxification of Reactive Oxygen Species.
R-MMU-5628897. TP53 Regulates Metabolic Genes.

Protein family/group databases

PeroxiBasei4453. MmPrxV.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-5, mitochondrial (EC:1.11.1.15)
Alternative name(s):
Antioxidant enzyme B166
Short name:
AOEB166
Liver tissue 2D-page spot 2D-0014IV
PLP
Peroxiredoxin V
Short name:
Prx-V
Peroxisomal antioxidant enzyme
Thioredoxin peroxidase PMP20
Thioredoxin reductase
Gene namesi
Name:Prdx5
Synonyms:Prdx6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1859821. Prdx5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic vesicle Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • peroxisomal matrix Source: UniProtKB
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48MitochondrionSequence analysisAdd BLAST48
ChainiPRO_000002379549 – 210Peroxiredoxin-5, mitochondrialAdd BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70N6-acetyllysine; alternateCombined sources1
Modified residuei70N6-succinyllysine; alternateCombined sources1
Modified residuei71N6-acetyllysineCombined sources1
Modified residuei79N6-acetyllysine; alternateCombined sources1
Modified residuei79N6-succinyllysine; alternateCombined sources1
Disulfide bondi96 ↔ 200Redox-activePROSITE-ProRule annotation
Modified residuei112N6-succinyllysineCombined sources1
Modified residuei167PhosphoserineBy similarity1
Modified residuei178PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP99029.
PaxDbiP99029.
PeptideAtlasiP99029.
PRIDEiP99029.

2D gel databases

REPRODUCTION-2DPAGEP99029.
SWISS-2DPAGEP99029.

PTM databases

iPTMnetiP99029.
PhosphoSitePlusiP99029.
SwissPalmiP99029.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSMUSG00000024953.
CleanExiMM_PRDX5.
MM_PRDX6.
ExpressionAtlasiP99029. baseline and differential.
GenevisibleiP99029. MM.

Interactioni

Subunit structurei

Monomer.

GO - Molecular functioni

  • protein dimerization activity Source: MGI
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi207718. 3 interactors.
IntActiP99029. 5 interactors.
MINTiMINT-1859227.
STRINGi10090.ENSMUSP00000025904.

Structurei

3D structure databases

ProteinModelPortaliP99029.
SMRiP99029.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 210ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi208 – 210Microbody targeting signalBy similarity3

Sequence similaritiesi

Belongs to the peroxiredoxin 2 family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0541. Eukaryota.
COG0678. LUCA.
GeneTreeiENSGT00390000018173.
HOGENOMiHOG000255884.
HOVERGENiHBG053675.
InParanoidiP99029.
KOiK11187.
OMAiCKFLGRV.
PhylomeDBiP99029.
TreeFamiTF105182.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF08534. Redoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P99029-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQLGLRVLG CKASSVLRAS TCLAGRAGRK EAGWECGGAR SFSSSAVTMA
60 70 80 90 100
PIKVGDAIPS VEVFEGEPGK KVNLAELFKG KKGVLFGVPG AFTPGCSKTH
110 120 130 140 150
LPGFVEQAGA LKAKGAQVVA CLSVNDVFVI EEWGRAHQAE GKVRLLADPT
160 170 180 190 200
GAFGKATDLL LDDSLVSLFG NRRLKRFSMV IDNGIVKALN VEPDGTGLTC
210
SLAPNILSQL
Length:210
Mass (Da):21,897
Last modified:February 21, 2002 - v2
Checksum:iE944104CC468BDD8
GO
Isoform Cytoplasmic+peroxisomal (identifier: P99029-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:162
Mass (Da):17,015
Checksum:iEE32FFB439418B21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55G → D AA sequence (Ref. 7) Curated1
Sequence conflicti83 – 102GVLFG…KTHLP → VFCLESLGHLHLAVLRPTA in AAF21016 (PubMed:10753630).CuratedAdd BLAST20

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188301 – 48Missing in isoform Cytoplasmic+peroxisomal. CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197951 mRNA. Translation: AAF04855.1.
AF124994 mRNA. Translation: AAF27532.1.
AF110733 mRNA. Translation: AAG13450.1.
AF208730, AF208729 Genomic DNA. Translation: AAF21016.1.
AK002383 mRNA. Translation: BAB22058.1.
AK003332 mRNA. Translation: BAB22720.1.
BC008174 mRNA. Translation: AAH08174.1.
CCDSiCCDS29507.1. [P99029-1]
PIRiJC7239.
RefSeqiNP_036151.1. NM_012021.2. [P99029-1]
UniGeneiMm.279782.

Genome annotation databases

EnsembliENSMUST00000025904; ENSMUSP00000025904; ENSMUSG00000024953. [P99029-1]
GeneIDi54683.
KEGGimmu:54683.
UCSCiuc008gjc.1. mouse. [P99029-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197951 mRNA. Translation: AAF04855.1.
AF124994 mRNA. Translation: AAF27532.1.
AF110733 mRNA. Translation: AAG13450.1.
AF208730, AF208729 Genomic DNA. Translation: AAF21016.1.
AK002383 mRNA. Translation: BAB22058.1.
AK003332 mRNA. Translation: BAB22720.1.
BC008174 mRNA. Translation: AAH08174.1.
CCDSiCCDS29507.1. [P99029-1]
PIRiJC7239.
RefSeqiNP_036151.1. NM_012021.2. [P99029-1]
UniGeneiMm.279782.

3D structure databases

ProteinModelPortaliP99029.
SMRiP99029.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207718. 3 interactors.
IntActiP99029. 5 interactors.
MINTiMINT-1859227.
STRINGi10090.ENSMUSP00000025904.

Protein family/group databases

PeroxiBasei4453. MmPrxV.

PTM databases

iPTMnetiP99029.
PhosphoSitePlusiP99029.
SwissPalmiP99029.

2D gel databases

REPRODUCTION-2DPAGEP99029.
SWISS-2DPAGEP99029.

Proteomic databases

MaxQBiP99029.
PaxDbiP99029.
PeptideAtlasiP99029.
PRIDEiP99029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025904; ENSMUSP00000025904; ENSMUSG00000024953. [P99029-1]
GeneIDi54683.
KEGGimmu:54683.
UCSCiuc008gjc.1. mouse. [P99029-1]

Organism-specific databases

CTDi25824.
MGIiMGI:1859821. Prdx5.

Phylogenomic databases

eggNOGiKOG0541. Eukaryota.
COG0678. LUCA.
GeneTreeiENSGT00390000018173.
HOGENOMiHOG000255884.
HOVERGENiHBG053675.
InParanoidiP99029.
KOiK11187.
OMAiCKFLGRV.
PhylomeDBiP99029.
TreeFamiTF105182.

Enzyme and pathway databases

ReactomeiR-MMU-3299685. Detoxification of Reactive Oxygen Species.
R-MMU-5628897. TP53 Regulates Metabolic Genes.

Miscellaneous databases

PROiP99029.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024953.
CleanExiMM_PRDX5.
MM_PRDX6.
ExpressionAtlasiP99029. baseline and differential.
GenevisibleiP99029. MM.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF08534. Redoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRDX5_MOUSE
AccessioniPrimary (citable) accession number: P99029
Secondary accession number(s): Q9QX45, Q9QZ75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2002
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.