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P99029

- PRDX5_MOUSE

UniProt

P99029 - PRDX5_MOUSE

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Protein

Peroxiredoxin-5, mitochondrial

Gene

Prdx5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 961Cysteine sulfenic acid (-SOH) intermediateSequence Analysis

GO - Molecular functioni

  1. antioxidant activity Source: UniProtKB
  2. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: Ensembl
  3. peroxidase activity Source: UniProtKB-KW
  4. peroxynitrite reductase activity Source: Ensembl
  5. receptor binding Source: UniProtKB
  6. RNA polymerase III regulatory region DNA binding Source: Ensembl

GO - Biological processi

  1. hydrogen peroxide catabolic process Source: UniProtKB
  2. NADPH oxidation Source: Ensembl
  3. negative regulation of apoptotic process Source: Ensembl
  4. negative regulation of oxidoreductase activity Source: Ensembl
  5. negative regulation of transcription from RNA polymerase III promoter Source: Ensembl
  6. positive regulation of collagen biosynthetic process Source: Ensembl
  7. reactive nitrogen species metabolic process Source: Ensembl
  8. regulation of apoptosis involved in tissue homeostasis Source: Ensembl
  9. response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiREACT_189141. Detoxification of Reactive Oxygen Species.

Protein family/group databases

PeroxiBasei4453. MmPrxV.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-5, mitochondrial (EC:1.11.1.15)
Alternative name(s):
Antioxidant enzyme B166
Short name:
AOEB166
Liver tissue 2D-page spot 2D-0014IV
PLP
Peroxiredoxin V
Short name:
Prx-V
Peroxisomal antioxidant enzyme
Thioredoxin peroxidase PMP20
Thioredoxin reductase
Gene namesi
Name:Prdx5
Synonyms:Prdx6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1859821. Prdx5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytoplasmic vesicle Source: Ensembl
  3. cytosol Source: Ensembl
  4. extracellular space Source: Ensembl
  5. extracellular vesicular exosome Source: Ensembl
  6. mitochondrion Source: MGI
  7. nucleus Source: Ensembl
  8. perinuclear region of cytoplasm Source: Ensembl
  9. peroxisomal matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848MitochondrionSequence AnalysisAdd
BLAST
Chaini49 – 210162Peroxiredoxin-5, mitochondrialPRO_0000023795Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701N6-acetyllysine; alternate1 Publication
Modified residuei70 – 701N6-succinyllysine; alternate1 Publication
Modified residuei71 – 711N6-acetyllysine1 Publication
Modified residuei79 – 791N6-acetyllysine; alternate1 Publication
Modified residuei79 – 791N6-succinyllysine; alternate1 Publication
Disulfide bondi96 ↔ 200Redox-activePROSITE-ProRule annotation
Modified residuei112 – 1121N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiP99029.
PaxDbiP99029.
PRIDEiP99029.

2D gel databases

REPRODUCTION-2DPAGEP99029.
SWISS-2DPAGEP99029.

PTM databases

PhosphoSiteiP99029.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiP99029.
CleanExiMM_PRDX5.
MM_PRDX6.
ExpressionAtlasiP99029. baseline and differential.
GenevestigatoriP99029.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi207718. 2 interactions.
IntActiP99029. 5 interactions.
MINTiMINT-1859227.

Structurei

3D structure databases

ProteinModelPortaliP99029.
SMRiP99029. Positions 50-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 210159ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi208 – 2103Microbody targeting signalBy similarity

Sequence similaritiesi

Belongs to the peroxiredoxin 2 family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiCOG0678.
GeneTreeiENSGT00390000018173.
HOGENOMiHOG000255884.
HOVERGENiHBG053675.
InParanoidiP99029.
KOiK11187.
OMAiMSAWGKQ.
OrthoDBiEOG77Q4XX.
PhylomeDBiP99029.
TreeFamiTF105182.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF08534. Redoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Mitochondrial (identifier: P99029-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQLGLRVLG CKASSVLRAS TCLAGRAGRK EAGWECGGAR SFSSSAVTMA
60 70 80 90 100
PIKVGDAIPS VEVFEGEPGK KVNLAELFKG KKGVLFGVPG AFTPGCSKTH
110 120 130 140 150
LPGFVEQAGA LKAKGAQVVA CLSVNDVFVI EEWGRAHQAE GKVRLLADPT
160 170 180 190 200
GAFGKATDLL LDDSLVSLFG NRRLKRFSMV IDNGIVKALN VEPDGTGLTC
210
SLAPNILSQL
Length:210
Mass (Da):21,897
Last modified:February 21, 2002 - v2
Checksum:iE944104CC468BDD8
GO
Isoform Cytoplasmic+peroxisomal (identifier: P99029-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:162
Mass (Da):17,015
Checksum:iEE32FFB439418B21
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551G → D AA sequence 1 PublicationCurated
Sequence conflicti83 – 10220GVLFG…KTHLP → VFCLESLGHLHLAVLRPTA in AAF21016. (PubMed:10753630)CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4848Missing in isoform Cytoplasmic+peroxisomal. CuratedVSP_018830Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197951 mRNA. Translation: AAF04855.1.
AF124994 mRNA. Translation: AAF27532.1.
AF110733 mRNA. Translation: AAG13450.1.
AF208730, AF208729 Genomic DNA. Translation: AAF21016.1.
AK002383 mRNA. Translation: BAB22058.1.
AK003332 mRNA. Translation: BAB22720.1.
BC008174 mRNA. Translation: AAH08174.1.
CCDSiCCDS29507.1. [P99029-1]
PIRiJC7239.
RefSeqiNP_036151.1. NM_012021.2. [P99029-1]
UniGeneiMm.279782.

Genome annotation databases

EnsembliENSMUST00000025904; ENSMUSP00000025904; ENSMUSG00000024953. [P99029-1]
GeneIDi54683.
KEGGimmu:54683.
UCSCiuc008gjc.1. mouse. [P99029-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197951 mRNA. Translation: AAF04855.1 .
AF124994 mRNA. Translation: AAF27532.1 .
AF110733 mRNA. Translation: AAG13450.1 .
AF208730 , AF208729 Genomic DNA. Translation: AAF21016.1 .
AK002383 mRNA. Translation: BAB22058.1 .
AK003332 mRNA. Translation: BAB22720.1 .
BC008174 mRNA. Translation: AAH08174.1 .
CCDSi CCDS29507.1. [P99029-1 ]
PIRi JC7239.
RefSeqi NP_036151.1. NM_012021.2. [P99029-1 ]
UniGenei Mm.279782.

3D structure databases

ProteinModelPortali P99029.
SMRi P99029. Positions 50-210.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207718. 2 interactions.
IntActi P99029. 5 interactions.
MINTi MINT-1859227.

Protein family/group databases

PeroxiBasei 4453. MmPrxV.

PTM databases

PhosphoSitei P99029.

2D gel databases

REPRODUCTION-2DPAGE P99029.
SWISS-2DPAGE P99029.

Proteomic databases

MaxQBi P99029.
PaxDbi P99029.
PRIDEi P99029.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025904 ; ENSMUSP00000025904 ; ENSMUSG00000024953 . [P99029-1 ]
GeneIDi 54683.
KEGGi mmu:54683.
UCSCi uc008gjc.1. mouse. [P99029-1 ]

Organism-specific databases

CTDi 25824.
MGIi MGI:1859821. Prdx5.

Phylogenomic databases

eggNOGi COG0678.
GeneTreei ENSGT00390000018173.
HOGENOMi HOG000255884.
HOVERGENi HBG053675.
InParanoidi P99029.
KOi K11187.
OMAi MSAWGKQ.
OrthoDBi EOG77Q4XX.
PhylomeDBi P99029.
TreeFami TF105182.

Enzyme and pathway databases

Reactomei REACT_189141. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

NextBioi 311536.
PROi P99029.
SOURCEi Search...

Gene expression databases

Bgeei P99029.
CleanExi MM_PRDX5.
MM_PRDX6.
ExpressionAtlasi P99029. baseline and differential.
Genevestigatori P99029.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF08534. Redoxin. 1 hit.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 1 hit.
PROSITEi PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse peroxiredoxin V is a thioredoxin peroxidase that inhibits p53-induced apoptosis."
    Zhou Y., Kok K.H., Chun A.C.S., Wong C.M., Wu H.W., Lin M.C.M., Fung P.C.W., Kung H.-F., Jin D.-Y.
    Biochem. Biophys. Res. Commun. 268:921-927(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
  2. "Characterization of human and murine PMP20 peroxisomal proteins that exhibit antioxidant activity in vitro."
    Yamashita H., Avraham S., Jiang S., London R., Van Veldhoven P.P., Subramani S., Rogers R.A., Avraham H.
    J. Biol. Chem. 274:29897-29904(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
  3. "Cloning and characterization of AOEB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family."
    Knoops B., Clippe A., Bogard C., Arsalane K., Wattiez R., Hermans C., Duconseille E., Falmagne P., Bernard A.
    J. Biol. Chem. 274:30451-30458(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
    Strain: C3H/HeJ.
    Tissue: Lung.
  4. "Molecular cloning and characterization of the mouse Peroxiredoxin V gene."
    Lee T.H., Kim S.J., Kang S.W., Lee K.K., Rhee S.G., Yu D.Y.
    Biochem. Biophys. Res. Commun. 270:356-362(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  7. Cited for: PROTEIN SEQUENCE OF 50-61.
    Tissue: Liver.
  8. Lubec G., Klug S., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 72-79; 83-112 AND 145-172, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain and Hippocampus.
  9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-70; LYS-79 AND LYS-112, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  10. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-70; LYS-71 AND LYS-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPRDX5_MOUSE
AccessioniPrimary (citable) accession number: P99029
Secondary accession number(s): Q9QX45, Q9QZ75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2002
Last modified: November 26, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3