Reviewed, UniProtKB/Swiss-Prot P99029 (PRDX5_MOUSE)

Names and origin

Protein names

Recommended name:
    Peroxiredoxin-5, mitochondrial
    EC=1.11.1.15
Alternative name(s):
    Prx-V
    Peroxisomal antioxidant enzyme
    PLP
    Thioredoxin reductase
    Thioredoxin peroxidase PMP20
    Antioxidant enzyme B166
      Short name=AOEB166
    Liver tissue 2D-page spot 2D-0014IV

Gene names

Name:	Prdx5
Synonyms:	Prdx6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.
Subunit structure	Monomer.
Subcellular location	Mitochondrion. Cytoplasm. Peroxisome.
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the peroxiredoxin 2 family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Peroxisome
Coding sequence diversity	Alternative initiation
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: MGI peroxisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Mitochondrial (identifier: P99029-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Cytoplasmic+peroxisomal (identifier: P99029-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Mitochondrion Potential
	Chain	49 – 210	162	Peroxiredoxin-5, mitochondrial		PRO_0000023795
Regions
	Domain	52 – 210	159	Thioredoxin
	Motif	208 – 210	3	Microbody targeting signal By similarity
Sites
	Active site	96	1	Cysteine sulfenic acid (-SOH) intermediate Potential
Amino acid modifications
	Disulfide bond	96 ↔ 200		Redox-active By similarity
Natural variations
	Alternative sequence	1 – 48	48	Missing in isoform Cytoplasmic+peroxisomal.		VSP_018830
Experimental info
	Sequence conflict	55	1	G → D AA sequence Ref.7
	Sequence conflict	83 – 102	20	GVLFG…KTHLP → VFCLESLGHLHLAVLRPTA in AAF21016. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Mitochondrial [UniParc]. Last modified February 21, 2002. Version 2. Checksum: E944104CC468BDD8	FASTA	210	21,897
	10 20 30 40 50 60 MLQLGLRVLG CKASSVLRAS TCLAGRAGRK EAGWECGGAR SFSSSAVTMA PIKVGDAIPS 70 80 90 100 110 120 VEVFEGEPGK KVNLAELFKG KKGVLFGVPG AFTPGCSKTH LPGFVEQAGA LKAKGAQVVA 130 140 150 160 170 180 CLSVNDVFVI EEWGRAHQAE GKVRLLADPT GAFGKATDLL LDDSLVSLFG NRRLKRFSMV 190 200 210 IDNGIVKALN VEPDGTGLTC SLAPNILSQL
	Isoform Cytoplasmic+peroxisomal [UniParc]. Checksum: EE32FFB439418B21		162	17,015
	10 20 30 40 50 60 MAPIKVGDAI PSVEVFEGEP GKKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA 70 80 90 100 110 120 GALKAKGAQV VACLSVNDVF VIEEWGRAHQ AEGKVRLLAD PTGAFGKATD LLLDDSLVSL 130 140 150 160 FGNRRLKRFS MVIDNGIVKA LNVEPDGTGL TCSLAPNILS QL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse peroxiredoxin V is a thioredoxin peroxidase that inhibits p53-induced apoptosis." Zhou Y., Kok K.H., Chun A.C.S., Wong C.M., Wu H.W., Lin M.C.M., Fung P.C.W., Kung H.-F., Jin D.-Y. Biochem. Biophys. Res. Commun. 268:921-927(2000) [PubMed: 10679306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	"Characterization of human and murine PMP20 peroxisomal proteins that exhibit antioxidant activity in vitro." Yamashita H., Avraham S., Jiang S., London R., Van Veldhoven P.P., Subramani S., Rogers R.A., Avraham H. J. Biol. Chem. 274:29897-29904(1999) [PubMed: 10514471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[3]	"Cloning and characterization of AOEB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family." Knoops B., Clippe A., Bogard C., Arsalane K., Wattiez R., Hermans C., Duconseille E., Falmagne P., Bernard A. J. Biol. Chem. 274:30451-30458(1999) [PubMed: 10521424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION. Strain: C3H/HeJ. Tissue: Lung.
[4]	"Molecular cloning and characterization of the mouse Peroxiredoxin V gene." Lee T.H., Kim S.J., Kang S.W., Lee K.K., Rhee S.G., Yu D.Y. Biochem. Biophys. Res. Commun. 270:356-362(2000) [PubMed: 10753630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[7]	Sanchez J.-C., Rouge V., Frutiger S., Hughes G.J., Yan J.X., Hoogland C., Appel R.D., Binz P.-A., Hochstrasser D.F., Cowthorne M. Submitted (AUG-1998) to UniProtKB Cited for: PROTEIN SEQUENCE OF 50-61. Tissue: Liver.
[8]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 72-79 AND 145-155, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[9]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 83-112 AND 156-172, MASS SPECTROMETRY. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF197951 mRNA. Translation: AAF04855.1. AF124994 mRNA. Translation: AAF27532.1. AF110733 mRNA. Translation: AAG13450.1. AF208730, AF208729 Genomic DNA. Translation: AAF21016.1. AK002383 mRNA. Translation: BAB22058.1. AK003332 mRNA. Translation: BAB22720.1. BC008174 mRNA. Translation: AAH08174.1.
PIR	JC7239.
RefSeq	NP_036151.1.
UniGene	Mm.279782
3D structure databases
HSSP	HSSP built from PDB template 1HD2 based on UniProtKB P30044.
SMR	P99029. Positions 50-210.
ModBase	Search...
Protein family/group databases
PeroxiBase	4453. MmPrxV.
PTM databases
PhosphoSite	P99029.
2-D gel databases
SWISS-2DPAGE	P99029.
REPRODUCTION-2DPAGE	P99029.
Genome annotation databases
Ensembl	ENSMUSG00000024953. Mus musculus. [Contig view]
GeneID	54683.
KEGG	mmu:54683.
Organism-specific databases
MGI	MGI:1859821. Prdx5.
Phylogenomic databases
HOGENOM	P99029.
HOVERGEN	P99029.
Gene expression databases
ArrayExpress	P99029.
CleanEx	MM_PRDX5. MM_PRDX6.
GermOnline	ENSMUSG00000024953. Mus musculus.
Family and domain databases
InterPro	IPR013740. Redoxin. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF08534. Redoxin. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	311536.
SOURCE	Search...

Entry information

Entry name

PRDX5_MOUSE

Accession

Primary (citable) accession number: P99029
Secondary accession number(s): Q9QX45, Q9QZ75

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 21, 2002
Last modified:	November 25, 2008
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)