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Protein

Peroxiredoxin-5, mitochondrial

Gene

Prdx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys Prx, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-5628897 TP53 Regulates Metabolic Genes

Protein family/group databases

PeroxiBasei4453 MmPrxV

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-5, mitochondrial (EC:1.11.1.151 Publication)
Alternative name(s):
Antioxidant enzyme B166
Short name:
AOEB166
Liver tissue 2D-page spot 2D-0014IV
PLP
Peroxiredoxin V
Short name:
Prx-V
Peroxisomal antioxidant enzyme
Thioredoxin peroxidase PMP20
Gene namesi
Name:Prdx5
Synonyms:Prdx6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1859821 Prdx5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48MitochondrionSequence analysisAdd BLAST48
ChainiPRO_000002379549 – 210Peroxiredoxin-5, mitochondrialAdd BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70N6-acetyllysine; alternateCombined sources1
Modified residuei70N6-succinyllysine; alternateCombined sources1
Modified residuei71N6-acetyllysineCombined sources1
Modified residuei79N6-acetyllysine; alternateCombined sources1
Modified residuei79N6-succinyllysine; alternateCombined sources1
Disulfide bondi96 ↔ 200Redox-activePROSITE-ProRule annotation
Modified residuei112N6-succinyllysineCombined sources1
Modified residuei167PhosphoserineBy similarity1
Modified residuei178PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP99029
PaxDbiP99029
PeptideAtlasiP99029
PRIDEiP99029

2D gel databases

REPRODUCTION-2DPAGEiP99029
SWISS-2DPAGEiP99029

PTM databases

iPTMnetiP99029
PhosphoSitePlusiP99029
SwissPalmiP99029

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSMUSG00000024953
CleanExiMM_PRDX5
MM_PRDX6
ExpressionAtlasiP99029 baseline and differential
GenevisibleiP99029 MM

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

  • protein dimerization activity Source: MGI
  • signaling receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi207718, 4 interactors
IntActiP99029, 5 interactors
MINTiP99029
STRINGi10090.ENSMUSP00000025904

Structurei

3D structure databases

ProteinModelPortaliP99029
SMRiP99029
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 210ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi208 – 210Microbody targeting signalBy similarity3

Sequence similaritiesi

Belongs to the peroxiredoxin family. Prx5 subfamily.Curated

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0541 Eukaryota
COG0678 LUCA
GeneTreeiENSGT00390000018173
HOGENOMiHOG000255884
HOVERGENiHBG053675
InParanoidiP99029
KOiK11187
OMAiQRYAMVV
PhylomeDBiP99029
TreeFamiTF105182

Family and domain databases

CDDicd03013 PRX5_like, 1 hit
InterProiView protein in InterPro
IPR037944 PRX5-like
IPR013740 Redoxin
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR10430 PTHR10430, 1 hit
PfamiView protein in Pfam
PF08534 Redoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: P99029-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQLGLRVLG CKASSVLRAS TCLAGRAGRK EAGWECGGAR SFSSSAVTMA
60 70 80 90 100
PIKVGDAIPS VEVFEGEPGK KVNLAELFKG KKGVLFGVPG AFTPGCSKTH
110 120 130 140 150
LPGFVEQAGA LKAKGAQVVA CLSVNDVFVI EEWGRAHQAE GKVRLLADPT
160 170 180 190 200
GAFGKATDLL LDDSLVSLFG NRRLKRFSMV IDNGIVKALN VEPDGTGLTC
210
SLAPNILSQL
Length:210
Mass (Da):21,897
Last modified:February 21, 2002 - v2
Checksum:iE944104CC468BDD8
GO
Isoform Cytoplasmic+peroxisomal (identifier: P99029-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:162
Mass (Da):17,015
Checksum:iEE32FFB439418B21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55G → D AA sequence (Ref. 7) Curated1
Sequence conflicti83 – 102GVLFG…KTHLP → VFCLESLGHLHLAVLRPTA in AAF21016 (PubMed:10753630).CuratedAdd BLAST20

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188301 – 48Missing in isoform Cytoplasmic+peroxisomal. CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197951 mRNA Translation: AAF04855.1
AF124994 mRNA Translation: AAF27532.1
AF110733 mRNA Translation: AAG13450.1
AF208730, AF208729 Genomic DNA Translation: AAF21016.1
AK002383 mRNA Translation: BAB22058.1
AK003332 mRNA Translation: BAB22720.1
BC008174 mRNA Translation: AAH08174.1
CCDSiCCDS29507.1 [P99029-1]
PIRiJC7239
RefSeqiNP_036151.1, NM_012021.2 [P99029-1]
UniGeneiMm.279782

Genome annotation databases

EnsembliENSMUST00000025904; ENSMUSP00000025904; ENSMUSG00000024953 [P99029-1]
GeneIDi54683
KEGGimmu:54683
UCSCiuc008gjc.1 mouse [P99029-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiPRDX5_MOUSE
AccessioniPrimary (citable) accession number: P99029
Secondary accession number(s): Q9QX45, Q9QZ75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2002
Last modified: May 23, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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