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Protein

Phospholipid-transporting ATPase 1

Gene

ALA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Has activity with phosphatidylserine and with a much lower efficiency with phosphatidylethanolamine, but not with phosphatidylcholine.1 Publication

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4574-aspartylphosphate intermediateBy similarity1
Metal bindingi859MagnesiumBy similarity1
Metal bindingi863MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G04930-MONOMER.
BRENDAi3.6.3.1. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.8.7. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase 11 Publication (EC:3.6.3.11 Publication)
Short name:
AtALA11 Publication
Alternative name(s):
Aminophospholipid flippase 11 Publication
Gene namesi
Name:ALA11 Publication
Ordered Locus Names:At5g04930Imported
ORF Names:MUG13.22Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G04930.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 100CytoplasmicSequence analysisAdd BLAST100
Transmembranei101 – 122HelicalSequence analysisAdd BLAST22
Topological domaini123 – 127ExtracellularSequence analysis5
Transmembranei128 – 150HelicalSequence analysisAdd BLAST23
Topological domaini151 – 329CytoplasmicSequence analysisAdd BLAST179
Transmembranei330 – 351HelicalSequence analysisAdd BLAST22
Topological domaini352 – 391ExtracellularSequence analysisAdd BLAST40
Transmembranei392 – 409HelicalSequence analysisAdd BLAST18
Topological domaini410 – 914CytoplasmicSequence analysisAdd BLAST505
Transmembranei915 – 934HelicalSequence analysisAdd BLAST20
Topological domaini935 – 948ExtracellularSequence analysisAdd BLAST14
Transmembranei949 – 968HelicalSequence analysisAdd BLAST20
Topological domaini969 – 998CytoplasmicSequence analysisAdd BLAST30
Transmembranei999 – 1020HelicalSequence analysisAdd BLAST22
Topological domaini1021 – 1027ExtracellularSequence analysis7
Transmembranei1028 – 1050HelicalSequence analysisAdd BLAST23
Topological domaini1051 – 1056CytoplasmicSequence analysis6
Transmembranei1057 – 1077HelicalSequence analysisAdd BLAST21
Topological domaini1078 – 1090ExtracellularSequence analysisAdd BLAST13
Transmembranei1091 – 1115HelicalSequence analysisAdd BLAST25
Topological domaini1116 – 1158CytoplasmicSequence analysisAdd BLAST43

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463851 – 1158Phospholipid-transporting ATPase 1Add BLAST1158

Proteomic databases

PaxDbiP98204.
PRIDEiP98204.

PTM databases

iPTMnetiP98204.

Expressioni

Tissue specificityi

Expressed in roots, flowers, anthers, leaves, vascular tissues and stems.1 Publication

Gene expression databases

GenevisibleiP98204. AT.

Interactioni

Protein-protein interaction databases

BioGridi15654. 1 interactor.
IntActiP98204. 1 interactor.
STRINGi3702.AT5G04930.1.

Structurei

3D structure databases

ProteinModelPortaliP98204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiP98204.
KOiK14802.
OMAiHAYSSDG.
OrthoDBiEOG093600M0.
PhylomeDBiP98204.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P98204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPRKSIDKP PHHDPILGVS SRWSVSSKDN KEVTFGDLGS KRIRHGSAGA
60 70 80 90 100
DSEMLSMSQK EIKDEDARLI YINDPDRTNE RFEFTGNSIK TAKYSVFTFL
110 120 130 140 150
PRNLFEQFHR VAYIYFLVIA VLNQLPQLAV FGRGASIMPL AFVLLVSAIK
160 170 180 190 200
DAYEDFRRHR SDRVENNRLA LVFEDHQFRE KKWKHIRVGE VIKVQSNQTL
210 220 230 240 250
PCDMVLLATS DPTGVVYVQT TNLDGESNLK TRYAKQETLL KAADMESFNG
260 270 280 290 300
FIKCEKPNRN IYGFQANMEI DGRRLSLGPS NIILRGCELK NTAWALGVVV
310 320 330 340 350
YAGGETKAML NNSGAPSKRS RLETRMNLEI ILLSLFLIVL CTIAAATAAV
360 370 380 390 400
WLRTHRDDLD TILFYRRKDY SERPGGKNYK YYGWGWEIFF TFFMAVIVYQ
410 420 430 440 450
IMIPISLYIS MELVRIGQAY FMTNDDQMYD ESSDSSFQCR ALNINEDLGQ
460 470 480 490 500
IKYLFSDKTG TLTDNKMEFQ CACIEGVDYS DREPADSEHP GYSIEVDGII
510 520 530 540 550
LKPKMRVRVD PVLLQLTKTG KATEEAKRAN EFFLSLAACN TIVPIVSNTS
560 570 580 590 600
DPNVKLVDYQ GESPDEQALV YAAAAYGFLL IERTSGHIVI NVRGETQRFN
610 620 630 640 650
VLGLHEFDSD RKRMSVILGC PDMSVKLFVK GADSSMFGVM DESYGGVIHE
660 670 680 690 700
TKIQLHAYSS DGLRTLVVGM RELNDSEFEQ WHSSFEAAST ALIGRAGLLR
710 720 730 740 750
KVAGNIETNL RIVGATAIED KLQRGVPEAI ESLRIAGIKV WVLTGDKQET
760 770 780 790 800
AISIGFSSRL LTRNMRQIVI NSNSLDSCRR SLEEANASIA SNDESDNVAL
810 820 830 840 850
IIDGTSLIYV LDNDLEDVLF QVACKCSAIL CCRVAPFQKA GIVALVKNRT
860 870 880 890 900
SDMTLAIGDG ANDVSMIQMA DVGVGISGQE GRQAVMASDF AMGQFRFLVP
910 920 930 940 950
LLLVHGHWNY QRMGYMILYN FYRNAVFVLI LFWYVLFTCY TLTTAITEWS
960 970 980 990 1000
SVLYSVIYTA IPTIIIGILD KDLGRQTLLD HPQLYGVGQR AEGYSTTLFW
1010 1020 1030 1040 1050
YTMIDTIWQS AAIFFIPMFA YWGSTIDTSS LGDLWTIAAV VVVNLHLAMD
1060 1070 1080 1090 1100
VIRWNWITHA AIWGSIVAAC ICVIVIDVIP TLPGYWAIFQ VGKTWMFWFC
1110 1120 1130 1140 1150
LLAIVVTSLL PRFAIKFLVE YYRPSDVRIA REAEKLGTFR ESQPVGVEMN

LIQDPPRR
Length:1,158
Mass (Da):130,329
Last modified:January 11, 2001 - v1
Checksum:i5CC042B40C8C974D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175769 mRNA. Translation: AAG01899.1.
AB005245 Genomic DNA. Translation: BAB11515.1.
CP002688 Genomic DNA. Translation: AED90805.1.
RefSeqiNP_568146.1. NM_120575.3.
UniGeneiAt.21675.

Genome annotation databases

EnsemblPlantsiAT5G04930.1; AT5G04930.1; AT5G04930.
GeneIDi830375.
GrameneiAT5G04930.1; AT5G04930.1; AT5G04930.
KEGGiath:AT5G04930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175769 mRNA. Translation: AAG01899.1.
AB005245 Genomic DNA. Translation: BAB11515.1.
CP002688 Genomic DNA. Translation: AED90805.1.
RefSeqiNP_568146.1. NM_120575.3.
UniGeneiAt.21675.

3D structure databases

ProteinModelPortaliP98204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15654. 1 interactor.
IntActiP98204. 1 interactor.
STRINGi3702.AT5G04930.1.

Protein family/group databases

TCDBi3.A.3.8.7. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP98204.

Proteomic databases

PaxDbiP98204.
PRIDEiP98204.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G04930.1; AT5G04930.1; AT5G04930.
GeneIDi830375.
GrameneiAT5G04930.1; AT5G04930.1; AT5G04930.
KEGGiath:AT5G04930.

Organism-specific databases

TAIRiAT5G04930.

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiP98204.
KOiK14802.
OMAiHAYSSDG.
OrthoDBiEOG093600M0.
PhylomeDBiP98204.

Enzyme and pathway databases

BioCyciARA:AT5G04930-MONOMER.
BRENDAi3.6.3.1. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP98204.

Gene expression databases

GenevisibleiP98204. AT.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALA1_ARATH
AccessioniPrimary (citable) accession number: P98204
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Knockdown mutants are cold sensitive.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.