Phospholipid-transporting ATPase 1 - Arabidopsis thaliana (Mouse-ear cress)

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P98204 (ALA1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phospholipid-transporting ATPase 1
Short name=AtALA1
EC=3.6.3.1

Alternative name(s):
Aminophospholipid flippase 1

Gene names

Name:	ALA1
Ordered Locus Names:	At5g04930
ORF Names:	MUG13.22

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1158 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in transport of phospholipids.
Catalytic activity	ATP + H₂O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cation transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1158

1158

Phospholipid-transporting ATPase 1

PRO_0000046385

Regions

Topological domain

1 – 100

100

Cytoplasmic Potential

Transmembrane

101 – 122

Helical; Potential

Topological domain

123 – 127

Extracellular Potential

Transmembrane

128 – 150

Helical; Potential

Topological domain

151 – 329

179

Cytoplasmic Potential

Transmembrane

330 – 351

Helical; Potential

Topological domain

352 – 391

Extracellular Potential

Transmembrane

392 – 409

Helical; Potential

Topological domain

410 – 914

505

Cytoplasmic Potential

Transmembrane

915 – 934

Helical; Potential

Topological domain

935 – 948

Extracellular Potential

Transmembrane

949 – 968

Helical; Potential

Topological domain

969 – 998

Cytoplasmic Potential

Transmembrane

999 – 1020

Helical; Potential

Topological domain

1021 – 1027

Extracellular Potential

Transmembrane

1028 – 1050

Helical; Potential

Topological domain

1051 – 1056

Cytoplasmic Potential

Transmembrane

1057 – 1077

Helical; Potential

Topological domain

1078 – 1090

Extracellular Potential

Transmembrane

1091 – 1115

Helical; Potential

Topological domain

1116 – 1158

Cytoplasmic Potential

Sites

Active site

457

4-aspartylphosphate intermediate By similarity

Metal binding

859

Magnesium By similarity

Metal binding

863

Magnesium By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P98204 [UniParc].

Last modified January 11, 2001. Version 1.
Checksum: 5CC042B40C8C974D
Show »

FASTA

1,158

130,329

        10         20         30         40         50         60 
MDPRKSIDKP PHHDPILGVS SRWSVSSKDN KEVTFGDLGS KRIRHGSAGA DSEMLSMSQK 

        70         80         90        100        110        120 
EIKDEDARLI YINDPDRTNE RFEFTGNSIK TAKYSVFTFL PRNLFEQFHR VAYIYFLVIA 

       130        140        150        160        170        180 
VLNQLPQLAV FGRGASIMPL AFVLLVSAIK DAYEDFRRHR SDRVENNRLA LVFEDHQFRE 

       190        200        210        220        230        240 
KKWKHIRVGE VIKVQSNQTL PCDMVLLATS DPTGVVYVQT TNLDGESNLK TRYAKQETLL 

       250        260        270        280        290        300 
KAADMESFNG FIKCEKPNRN IYGFQANMEI DGRRLSLGPS NIILRGCELK NTAWALGVVV 

       310        320        330        340        350        360 
YAGGETKAML NNSGAPSKRS RLETRMNLEI ILLSLFLIVL CTIAAATAAV WLRTHRDDLD 

       370        380        390        400        410        420 
TILFYRRKDY SERPGGKNYK YYGWGWEIFF TFFMAVIVYQ IMIPISLYIS MELVRIGQAY 

       430        440        450        460        470        480 
FMTNDDQMYD ESSDSSFQCR ALNINEDLGQ IKYLFSDKTG TLTDNKMEFQ CACIEGVDYS 

       490        500        510        520        530        540 
DREPADSEHP GYSIEVDGII LKPKMRVRVD PVLLQLTKTG KATEEAKRAN EFFLSLAACN 

       550        560        570        580        590        600 
TIVPIVSNTS DPNVKLVDYQ GESPDEQALV YAAAAYGFLL IERTSGHIVI NVRGETQRFN 

       610        620        630        640        650        660 
VLGLHEFDSD RKRMSVILGC PDMSVKLFVK GADSSMFGVM DESYGGVIHE TKIQLHAYSS 

       670        680        690        700        710        720 
DGLRTLVVGM RELNDSEFEQ WHSSFEAAST ALIGRAGLLR KVAGNIETNL RIVGATAIED 

       730        740        750        760        770        780 
KLQRGVPEAI ESLRIAGIKV WVLTGDKQET AISIGFSSRL LTRNMRQIVI NSNSLDSCRR 

       790        800        810        820        830        840 
SLEEANASIA SNDESDNVAL IIDGTSLIYV LDNDLEDVLF QVACKCSAIL CCRVAPFQKA 

       850        860        870        880        890        900 
GIVALVKNRT SDMTLAIGDG ANDVSMIQMA DVGVGISGQE GRQAVMASDF AMGQFRFLVP 

       910        920        930        940        950        960 
LLLVHGHWNY QRMGYMILYN FYRNAVFVLI LFWYVLFTCY TLTTAITEWS SVLYSVIYTA 

       970        980        990       1000       1010       1020 
IPTIIIGILD KDLGRQTLLD HPQLYGVGQR AEGYSTTLFW YTMIDTIWQS AAIFFIPMFA 

      1030       1040       1050       1060       1070       1080 
YWGSTIDTSS LGDLWTIAAV VVVNLHLAMD VIRWNWITHA AIWGSIVAAC ICVIVIDVIP 

      1090       1100       1110       1120       1130       1140 
TLPGYWAIFQ VGKTWMFWFC LLAIVVTSLL PRFAIKFLVE YYRPSDVRIA REAEKLGTFR 

      1150 
ESQPVGVEMN LIQDPPRR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chilling tolerance in Arabidopsis involves ALA1, a member of a new family of putative aminophospholipid translocases." Gomes E., Jakobsen M.K., Axelsen K.B., Geisler M., Palmgren M.G. Plant Cell 12:2441-2454(2000) [PubMed: 11148289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF175769 mRNA. Translation: AAG01899.1. AB005245 Genomic DNA. Translation: BAB11515.1. CP002688 Genomic DNA. Translation: AED90805.1.
IPI	IPI00524087.
RefSeq	NP_568146.1. NM_120575.2.
UniGene	At.21675.
3D structure databases
ProteinModelPortal	P98204.
SMR	P98204. Positions 837-878.
ModBase	Search...
Protein-protein interaction databases
IntAct	P98204. 1 interaction.
STRING	P98204.
Protein family/group databases
TCDB	3.A.3.8.7. P-type ATPase (P-ATPase) superfamily.
Proteomic databases
PRIDE	P98204.
ProMEX	P98204.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G04930.1; AT5G04930.1; AT5G04930.
GeneID	830375.
GenomeReviews	Gene locus AT5G04930 in contig BA000015_GR.
KEGG	ath:AT5G04930.
NMPDR	fig\|3702.1.peg.22591.
Organism-specific databases
TAIR	At5g04930.
Phylogenomic databases
eggNOG	KOG0206.
HOGENOM	HBG745019.
InParanoid	P98204.
OMA	NERFEFT.
PhylomeDB	P98204.
ProtClustDB	PLN03190.
Gene expression databases
ArrayExpress	P98204.
Genevestigator	P98204.
GermOnline	AT5G04930. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 2 hits. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
Pfam	PF00122. E1-E2_ATPase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALA1_ARATH

Accession

Primary (citable) accession number: P98204

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents