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Protein

Phospholipid-transporting ATPase 1

Gene

ALA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Has activity with phosphatidylserine and with a much lower efficiency with phosphatidylethanolamine, but not with phosphatidylcholine.1 Publication

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei457 – 45714-aspartylphosphate intermediateBy similarity
Metal bindingi859 – 8591MagnesiumBy similarity
Metal bindingi863 – 8631MagnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G04930-MONOMER.
BRENDAi3.6.3.1. 399.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.8.7. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase 11 Publication (EC:3.6.3.11 Publication)
Short name:
AtALA11 Publication
Alternative name(s):
Aminophospholipid flippase 11 Publication
Gene namesi
Name:ALA11 Publication
Ordered Locus Names:At5g04930Imported
ORF Names:MUG13.22Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G04930.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 100100CytoplasmicSequence analysisAdd
BLAST
Transmembranei101 – 12222HelicalSequence analysisAdd
BLAST
Topological domaini123 – 1275ExtracellularSequence analysis
Transmembranei128 – 15023HelicalSequence analysisAdd
BLAST
Topological domaini151 – 329179CytoplasmicSequence analysisAdd
BLAST
Transmembranei330 – 35122HelicalSequence analysisAdd
BLAST
Topological domaini352 – 39140ExtracellularSequence analysisAdd
BLAST
Transmembranei392 – 40918HelicalSequence analysisAdd
BLAST
Topological domaini410 – 914505CytoplasmicSequence analysisAdd
BLAST
Transmembranei915 – 93420HelicalSequence analysisAdd
BLAST
Topological domaini935 – 94814ExtracellularSequence analysisAdd
BLAST
Transmembranei949 – 96820HelicalSequence analysisAdd
BLAST
Topological domaini969 – 99830CytoplasmicSequence analysisAdd
BLAST
Transmembranei999 – 102022HelicalSequence analysisAdd
BLAST
Topological domaini1021 – 10277ExtracellularSequence analysis
Transmembranei1028 – 105023HelicalSequence analysisAdd
BLAST
Topological domaini1051 – 10566CytoplasmicSequence analysis
Transmembranei1057 – 107721HelicalSequence analysisAdd
BLAST
Topological domaini1078 – 109013ExtracellularSequence analysisAdd
BLAST
Transmembranei1091 – 111525HelicalSequence analysisAdd
BLAST
Topological domaini1116 – 115843CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11581158Phospholipid-transporting ATPase 1PRO_0000046385Add
BLAST

Proteomic databases

PaxDbiP98204.
PRIDEiP98204.

PTM databases

iPTMnetiP98204.

Expressioni

Tissue specificityi

Expressed in roots, flowers, anthers, leaves, vascular tissues and stems.1 Publication

Gene expression databases

GenevisibleiP98204. AT.

Interactioni

Protein-protein interaction databases

BioGridi15654. 1 interaction.
IntActiP98204. 1 interaction.
STRINGi3702.AT5G04930.1.

Structurei

3D structure databases

ProteinModelPortaliP98204.
SMRiP98204. Positions 679-881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiP98204.
KOiK14802.
OMAiHAYSSDG.
PhylomeDBiP98204.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P98204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPRKSIDKP PHHDPILGVS SRWSVSSKDN KEVTFGDLGS KRIRHGSAGA
60 70 80 90 100
DSEMLSMSQK EIKDEDARLI YINDPDRTNE RFEFTGNSIK TAKYSVFTFL
110 120 130 140 150
PRNLFEQFHR VAYIYFLVIA VLNQLPQLAV FGRGASIMPL AFVLLVSAIK
160 170 180 190 200
DAYEDFRRHR SDRVENNRLA LVFEDHQFRE KKWKHIRVGE VIKVQSNQTL
210 220 230 240 250
PCDMVLLATS DPTGVVYVQT TNLDGESNLK TRYAKQETLL KAADMESFNG
260 270 280 290 300
FIKCEKPNRN IYGFQANMEI DGRRLSLGPS NIILRGCELK NTAWALGVVV
310 320 330 340 350
YAGGETKAML NNSGAPSKRS RLETRMNLEI ILLSLFLIVL CTIAAATAAV
360 370 380 390 400
WLRTHRDDLD TILFYRRKDY SERPGGKNYK YYGWGWEIFF TFFMAVIVYQ
410 420 430 440 450
IMIPISLYIS MELVRIGQAY FMTNDDQMYD ESSDSSFQCR ALNINEDLGQ
460 470 480 490 500
IKYLFSDKTG TLTDNKMEFQ CACIEGVDYS DREPADSEHP GYSIEVDGII
510 520 530 540 550
LKPKMRVRVD PVLLQLTKTG KATEEAKRAN EFFLSLAACN TIVPIVSNTS
560 570 580 590 600
DPNVKLVDYQ GESPDEQALV YAAAAYGFLL IERTSGHIVI NVRGETQRFN
610 620 630 640 650
VLGLHEFDSD RKRMSVILGC PDMSVKLFVK GADSSMFGVM DESYGGVIHE
660 670 680 690 700
TKIQLHAYSS DGLRTLVVGM RELNDSEFEQ WHSSFEAAST ALIGRAGLLR
710 720 730 740 750
KVAGNIETNL RIVGATAIED KLQRGVPEAI ESLRIAGIKV WVLTGDKQET
760 770 780 790 800
AISIGFSSRL LTRNMRQIVI NSNSLDSCRR SLEEANASIA SNDESDNVAL
810 820 830 840 850
IIDGTSLIYV LDNDLEDVLF QVACKCSAIL CCRVAPFQKA GIVALVKNRT
860 870 880 890 900
SDMTLAIGDG ANDVSMIQMA DVGVGISGQE GRQAVMASDF AMGQFRFLVP
910 920 930 940 950
LLLVHGHWNY QRMGYMILYN FYRNAVFVLI LFWYVLFTCY TLTTAITEWS
960 970 980 990 1000
SVLYSVIYTA IPTIIIGILD KDLGRQTLLD HPQLYGVGQR AEGYSTTLFW
1010 1020 1030 1040 1050
YTMIDTIWQS AAIFFIPMFA YWGSTIDTSS LGDLWTIAAV VVVNLHLAMD
1060 1070 1080 1090 1100
VIRWNWITHA AIWGSIVAAC ICVIVIDVIP TLPGYWAIFQ VGKTWMFWFC
1110 1120 1130 1140 1150
LLAIVVTSLL PRFAIKFLVE YYRPSDVRIA REAEKLGTFR ESQPVGVEMN

LIQDPPRR
Length:1,158
Mass (Da):130,329
Last modified:January 11, 2001 - v1
Checksum:i5CC042B40C8C974D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175769 mRNA. Translation: AAG01899.1.
AB005245 Genomic DNA. Translation: BAB11515.1.
CP002688 Genomic DNA. Translation: AED90805.1.
RefSeqiNP_568146.1. NM_120575.2.
UniGeneiAt.21675.

Genome annotation databases

EnsemblPlantsiAT5G04930.1; AT5G04930.1; AT5G04930.
GeneIDi830375.
GrameneiAT5G04930.1; AT5G04930.1; AT5G04930.
KEGGiath:AT5G04930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175769 mRNA. Translation: AAG01899.1.
AB005245 Genomic DNA. Translation: BAB11515.1.
CP002688 Genomic DNA. Translation: AED90805.1.
RefSeqiNP_568146.1. NM_120575.2.
UniGeneiAt.21675.

3D structure databases

ProteinModelPortaliP98204.
SMRiP98204. Positions 679-881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15654. 1 interaction.
IntActiP98204. 1 interaction.
STRINGi3702.AT5G04930.1.

Protein family/group databases

TCDBi3.A.3.8.7. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP98204.

Proteomic databases

PaxDbiP98204.
PRIDEiP98204.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G04930.1; AT5G04930.1; AT5G04930.
GeneIDi830375.
GrameneiAT5G04930.1; AT5G04930.1; AT5G04930.
KEGGiath:AT5G04930.

Organism-specific databases

TAIRiAT5G04930.

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiP98204.
KOiK14802.
OMAiHAYSSDG.
PhylomeDBiP98204.

Enzyme and pathway databases

BioCyciARA:AT5G04930-MONOMER.
BRENDAi3.6.3.1. 399.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP98204.

Gene expression databases

GenevisibleiP98204. AT.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Chilling tolerance in Arabidopsis involves ALA1, a member of a new family of putative aminophospholipid translocases."
    Gomes E., Jakobsen M.K., Axelsen K.B., Geisler M., Palmgren M.G.
    Plant Cell 12:2441-2454(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, FUNCTION, TISSUE SPECIFICITY.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Inventory of the superfamily of P-type ion pumps in Arabidopsis."
    Axelsen K.B., Palmgren M.G.
    Plant Physiol. 126:696-706(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "A putative plant aminophospholipid flippase, the Arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a beta-subunit."
    Lopez-Marques R.L., Poulsen L.R., Palmgren M.G.
    PLoS ONE 7:E33042-E33042(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiALA1_ARATH
AccessioniPrimary (citable) accession number: P98204
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: July 6, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Knockdown mutants are cold sensitive.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.