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Protein

Phospholipid-transporting ATPase ID

Gene

Atp8b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).By similarity

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4114-aspartylphosphate intermediateBy similarity1
Metal bindingi833MagnesiumBy similarity1
Metal bindingi837MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase ID (EC:3.6.3.1)
Alternative name(s):
ATPase class I type 8B member 2
P4-ATPase flippase complex alpha subunit ATP8B2
Gene namesi
Name:Atp8b2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1859660. Atp8b2.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis

  • Note: TMEM30A, but not TMEM30B, is required for its export from endoplasmic reticulum to the plasma membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 68CytoplasmicSequence analysisAdd BLAST68
Transmembranei69 – 89HelicalSequence analysisAdd BLAST21
Topological domaini90 – 91Exoplasmic loopSequence analysis2
Transmembranei92 – 112HelicalSequence analysisAdd BLAST21
Topological domaini113 – 295CytoplasmicSequence analysisAdd BLAST183
Transmembranei296 – 316HelicalSequence analysisAdd BLAST21
Topological domaini317 – 338Exoplasmic loopSequence analysisAdd BLAST22
Transmembranei339 – 359HelicalSequence analysisAdd BLAST21
Topological domaini360 – 898CytoplasmicSequence analysisAdd BLAST539
Transmembranei899 – 919HelicalSequence analysisAdd BLAST21
Topological domaini920 – 922Exoplasmic loopSequence analysis3
Transmembranei923 – 943HelicalSequence analysisAdd BLAST21
Topological domaini944 – 972CytoplasmicSequence analysisAdd BLAST29
Transmembranei973 – 993HelicalSequence analysisAdd BLAST21
Topological domaini994 – 1011Exoplasmic loopSequence analysisAdd BLAST18
Transmembranei1012 – 1032HelicalSequence analysisAdd BLAST21
Topological domaini1033 – 1036CytoplasmicSequence analysis4
Transmembranei1037 – 1057HelicalSequence analysisAdd BLAST21
Topological domaini1058 – 1082Exoplasmic loopSequence analysisAdd BLAST25
Transmembranei1083 – 1103HelicalSequence analysisAdd BLAST21
Topological domaini1104 – 1209CytoplasmicSequence analysisAdd BLAST106

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463661 – 1209Phospholipid-transporting ATPase IDAdd BLAST1209

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1175PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP98199.
MaxQBiP98199.
PaxDbiP98199.
PRIDEiP98199.

PTM databases

iPTMnetiP98199.
PhosphoSitePlusiP98199.

Expressioni

Gene expression databases

BgeeiENSMUSG00000060671.
CleanExiMM_ATP8B2.
ExpressionAtlasiP98199. baseline and differential.
GenevisibleiP98199. MM.

Interactioni

Subunit structurei

Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit (Probable). The probable flippase ATP8B2:TMEM30A complex can form an intermediate phosphoenzyme in vitro (By similarity).By similarityCurated

Protein-protein interaction databases

BioGridi207713. 1 interactor.
STRINGi10090.ENSMUSP00000103019.

Structurei

3D structure databases

ProteinModelPortaliP98199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0206. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00860000133706.
HOGENOMiHOG000202528.
HOVERGENiHBG050601.
InParanoidiP98199.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030347. ATP8B2.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF46. PTHR24092:SF46. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P98199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVPKEIPEK WARAGAPPSW SQKKPSWGTE EERRARANDR EYNEKFQYAS
60 70 80 90 100
NCIKTSKYNI VTFLPVNLFE QFQEVANTYF LFLLILQLIP QISSLSWFTT
110 120 130 140 150
IVPLVLVLTI TAVKDATDDY FRHKSDNQVN NRHSQVLING VLQQEQWMNV
160 170 180 190 200
CVGDIIKLEN NQFVAADLLL LSSSEPHGLC YIETAELDGE TNMKVRQAIP
210 220 230 240 250
VTSELGDVSQ LARFDGEVIC EPPNNKLDKF SGTLYWKENK FPLSNQNMLL
260 270 280 290 300
RGCVLRNTEW CFGLVIFAGP DTKLMQNSGR TKFKRTSIDR LMNTLVLWIF
310 320 330 340 350
GFLVCMGVIL AIGNAIWEHE VGTRFQVYLP WDEAVDSAFF SGFLSFWSYI
360 370 380 390 400
IILNTVVPIS LYVSVEVIRL GHSYFINWDK KMFCMKKRTP AEARTTTLNE
410 420 430 440 450
ELGQVEYIFS DKTGTLTQNI MVFNKCSING HSYGDVFDVL GHKAELGERP
460 470 480 490 500
EPVDFSFNPL ADKKFLFWDS SLLEAVKMGD PHTHEFFRLL SLCHTVMSEE
510 520 530 540 550
KNEGELYYKA QSPDEGALVT AARNFGFVFR SRTPKTITVH ELGTAITYQL
560 570 580 590 600
LAILDFNNIR KRMSVIVRNP EGKIRLYCKG ADTILLDRLH PPTQELLSST
610 620 630 640 650
TDHLNEYAGD GLRTLVLAYK DLDEEYYEEW ARRRLQASLA QDSREDRLAS
660 670 680 690 700
IYEEVESDMM LLGATAIEDK LQQGVPETIA LLTLANIKIW VLTGDKQETA
710 720 730 740 750
VNIGYSCKML TDDMTEVFVV TGHTVLEVRE ELRKARKKMV DSSHAVGNGF
760 770 780 790 800
TYQGNLSSSK LTSVLEAVAG EYALVINGHS LAHALEADME LEFLETACAC
810 820 830 840 850
KAVICCRVTP LQKAQVVELV KKYKKAVTLA IGDGANDVSM IKTAHIGVGI
860 870 880 890 900
SGQEGIQAVL ASDYSFSQFK FLQRLLLVHG RWSYLRMCKF LCYFFYKNFA
910 920 930 940 950
FTMVHFWFGF FCGFSAQTVY DQYFITLYNI VYTSLPVLAM GVFDQDVPEQ
960 970 980 990 1000
RSMEYPKLYE PGQLNLLFNK REFFICIAQG IYTSVLMFFI PYGVFAEATR
1010 1020 1030 1040 1050
DDGTQLADYQ SFAVTVATSL VIVVSVQIGL DTGYWTAINH FFIWGSLAVY
1060 1070 1080 1090 1100
FAILFAMHSN GLFDMFPNQF RFVGNAQNTL AQPTVWLTIA LTTAVCIMPV
1110 1120 1130 1140 1150
VAFRFLRLSL KPDLSDTVRY TQLVRKKQKA QHRCMRRVGR TGSRRSGYAF
1160 1170 1180 1190 1200
SHQEGFGELI MSGKNMRLSS LALSSFSTRS SSSWIESLRR KKSDSANSPS

GGAEKPLKG
Length:1,209
Mass (Da):136,968
Last modified:January 9, 2007 - v2
Checksum:i4722C6D25F280273
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti989F → L in AAF09444 (PubMed:11015572).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC163616 Genomic DNA. No translation available.
AF156546 mRNA. Translation: AAF09444.1.
RefSeqiXP_006501777.1. XM_006501714.3.
UniGeneiMm.30455.

Genome annotation databases

EnsembliENSMUST00000069805; ENSMUSP00000063384; ENSMUSG00000060671.
GeneIDi54667.
UCSCiuc008qah.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC163616 Genomic DNA. No translation available.
AF156546 mRNA. Translation: AAF09444.1.
RefSeqiXP_006501777.1. XM_006501714.3.
UniGeneiMm.30455.

3D structure databases

ProteinModelPortaliP98199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207713. 1 interactor.
STRINGi10090.ENSMUSP00000103019.

PTM databases

iPTMnetiP98199.
PhosphoSitePlusiP98199.

Proteomic databases

EPDiP98199.
MaxQBiP98199.
PaxDbiP98199.
PRIDEiP98199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069805; ENSMUSP00000063384; ENSMUSG00000060671.
GeneIDi54667.
UCSCiuc008qah.1. mouse.

Organism-specific databases

CTDi57198.
MGIiMGI:1859660. Atp8b2.

Phylogenomic databases

eggNOGiKOG0206. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00860000133706.
HOGENOMiHOG000202528.
HOVERGENiHBG050601.
InParanoidiP98199.

Enzyme and pathway databases

ReactomeiR-MMU-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP98199.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060671.
CleanExiMM_ATP8B2.
ExpressionAtlasiP98199. baseline and differential.
GenevisibleiP98199. MM.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030347. ATP8B2.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF46. PTHR24092:SF46. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT8B2_MOUSE
AccessioniPrimary (citable) accession number: P98199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 9, 2007
Last modified: November 30, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.