P98195 (ATP9B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phospholipid-transporting ATPase IIB
EC=3.6.3.1

Alternative name(s):
ATPase class II type 9B

Gene names

Name:

Atp9b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1146 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Found in most tissues except spleen and muscle. Most abundant in testis. Also detected in fetal tissues.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cation transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P98195-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P98195-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1091-1101: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1146	1146	Probable phospholipid-transporting ATPase IIB		PRO_0000046378
Regions
	Topological domain	1 – 143	143	Cytoplasmic Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Topological domain	165 – 172	8	Extracellular Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Topological domain	194 – 381	188	Cytoplasmic Potential
	Transmembrane	382 – 402	21	Helical; Potential
	Topological domain	403 – 407	5	Extracellular Potential
	Transmembrane	408 – 427	20	Helical; Potential
	Topological domain	428 – 938	511	Cytoplasmic Potential
	Transmembrane	939 – 959	21	Helical; Potential
	Topological domain	960 – 961	2	Extracellular Potential
	Transmembrane	962 – 982	21	Helical; Potential
	Topological domain	983 – 1011	29	Cytoplasmic Potential
	Transmembrane	1012 – 1032	21	Helical; Potential
	Topological domain	1033 – 1040	8	Extracellular Potential
	Transmembrane	1041 – 1061	21	Helical; Potential
	Topological domain	1062 – 1065	4	Cytoplasmic Potential
	Transmembrane	1066 – 1086	21	Helical; Potential
	Topological domain	1087 – 1105	19	Extracellular Potential
	Transmembrane	1106 – 1128	23	Helical; Potential
	Topological domain	1129 – 1146	18	Cytoplasmic Potential
Sites
	Active site	467	1	4-aspartylphosphate intermediate By similarity
	Metal binding	873	1	Magnesium By similarity
	Metal binding	877	1	Magnesium By similarity
Natural variations
	Alternative sequence	1091 – 1101	11	Missing in isoform 2.		VSP_035908
Experimental info
	Sequence conflict	366	1	N → K in AAH79626. Ref.1
	Sequence conflict	605	1	V → I in AAH79626. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 16, 2008. Version 4. Checksum: 334AAEE291DFDC2E	FASTA	1,146	129,017
	10 20 30 40 50 60 MADQIPLYPV RSAGAAASHR RAAYYSSAGP GPGADRRGRY QLEDESAHLD EMPLMMSEEG 70 80 90 100 110 120 FENDESDYHT LPRARITRRK RGLEWFVCGG WKFLCTSCCD WLINVCQRKK ELKARTVWLG 130 140 150 160 170 180 CPEKCEEKHP RNSIKNQKYN VFTFIPGVLY EQFKFFLNLY FLVVSCSQFV PALKIGYLYT 190 200 210 220 230 240 YWAPLGFVLA VTIAREAIDE FRRFQRDKEM NSQLYSKLTV RGKVQVKSSD IQVGDLIIVE 250 260 270 280 290 300 KNQRIPSDMV FLRTSEKAGS CFIRTDQLDG ETDWKLKVAV SCTQRLPALG DLFSISAYVY 310 320 330 340 350 360 AQKPQLDIHS FEGTFTREDS DPPIHESLSI ENTLWASTIV ASGTVIGVVI YTGKETRSVM 370 380 390 400 410 420 NTSNPNNKVG LLDLELNQLT KALFLALVVL SVVMVTLQGF AGPWYRNLFR FLLLFSYIIP 430 440 450 460 470 480 ISLRVNLDMG KAAYGWMIMK DENIPGTVVR TSTIPEELGR LVYLLTDKTG TLTQNEMVFK 490 500 510 520 530 540 RLHLGTVSYG TDTMDEIQSH VLNSYLQVHS QPSGHNPSSA PLRRSQSSTP KVKKSVSSRI 550 560 570 580 590 600 HEAVKAIALC HNVTPVYEAR AGITGETEFA EADQDFSDEN RTYQASSPDE VALVRWTESV 610 620 630 640 650 660 GLTLVSRDLA SMQLKTPSGQ VLTYCILQMF PFTSESKRMG IIVRDESTAE ITFYMKGADV 670 680 690 700 710 720 AMSTIVQYND WLEEECGNMA REGLRTLVVA KRTLTEEQYQ DFESRYSQAK LSIHDRALKV 730 740 750 760 770 780 AAVVESLERE MELLCLTGVE DQLQADVRPT LEMLRNAGIK IWMLTGDKLE TATCIAKSSH 790 800 810 820 830 840 LVSRTQDIHV FRPVTSRGEA HLELNAFRRK HDCALVISGD SLEVCLRYYE HELVELACQC 850 860 870 880 890 900 PAVVCCRCSP TQKAHIVTLL RQHTRKRTCA IGDGGNDVSM IQAADCGIGI EGKEGKQASL 910 920 930 940 950 960 AADFSITQFR HIGRLLMVHG RNSYKRSAAL GQFVMHRGLI ISTMQAVFSS VFYFASVPLY 970 980 990 1000 1010 1020 QGFLMVGYAT IYTMFPVFSL VLDQDVKPEM AILYPELYKD LTKGRSLSFK TFLIWVLISI 1030 1040 1050 1060 1070 1080 YQGGILMYGA LLLFEDEFVH VVAISFTALI LTELLMVALT IRTWHWLMVV AEFLSLGCYV 1090 1100 1110 1120 1130 1140 ASLAFLNEYF GIGRVSFGAF LDVAFITTVT FLWKVSAITV VSCLPLYVLK YLKRKLSPPS YSKLSS
	Isoform 2 [UniParc]. Checksum: FF41C197C7AA7D58	FASTA	1,135	127,912
	10 20 30 40 50 60 MADQIPLYPV RSAGAAASHR RAAYYSSAGP GPGADRRGRY QLEDESAHLD EMPLMMSEEG 70 80 90 100 110 120 FENDESDYHT LPRARITRRK RGLEWFVCGG WKFLCTSCCD WLINVCQRKK ELKARTVWLG 130 140 150 160 170 180 CPEKCEEKHP RNSIKNQKYN VFTFIPGVLY EQFKFFLNLY FLVVSCSQFV PALKIGYLYT 190 200 210 220 230 240 YWAPLGFVLA VTIAREAIDE FRRFQRDKEM NSQLYSKLTV RGKVQVKSSD IQVGDLIIVE 250 260 270 280 290 300 KNQRIPSDMV FLRTSEKAGS CFIRTDQLDG ETDWKLKVAV SCTQRLPALG DLFSISAYVY 310 320 330 340 350 360 AQKPQLDIHS FEGTFTREDS DPPIHESLSI ENTLWASTIV ASGTVIGVVI YTGKETRSVM 370 380 390 400 410 420 NTSNPNNKVG LLDLELNQLT KALFLALVVL SVVMVTLQGF AGPWYRNLFR FLLLFSYIIP 430 440 450 460 470 480 ISLRVNLDMG KAAYGWMIMK DENIPGTVVR TSTIPEELGR LVYLLTDKTG TLTQNEMVFK 490 500 510 520 530 540 RLHLGTVSYG TDTMDEIQSH VLNSYLQVHS QPSGHNPSSA PLRRSQSSTP KVKKSVSSRI 550 560 570 580 590 600 HEAVKAIALC HNVTPVYEAR AGITGETEFA EADQDFSDEN RTYQASSPDE VALVRWTESV 610 620 630 640 650 660 GLTLVSRDLA SMQLKTPSGQ VLTYCILQMF PFTSESKRMG IIVRDESTAE ITFYMKGADV 670 680 690 700 710 720 AMSTIVQYND WLEEECGNMA REGLRTLVVA KRTLTEEQYQ DFESRYSQAK LSIHDRALKV 730 740 750 760 770 780 AAVVESLERE MELLCLTGVE DQLQADVRPT LEMLRNAGIK IWMLTGDKLE TATCIAKSSH 790 800 810 820 830 840 LVSRTQDIHV FRPVTSRGEA HLELNAFRRK HDCALVISGD SLEVCLRYYE HELVELACQC 850 860 870 880 890 900 PAVVCCRCSP TQKAHIVTLL RQHTRKRTCA IGDGGNDVSM IQAADCGIGI EGKEGKQASL 910 920 930 940 950 960 AADFSITQFR HIGRLLMVHG RNSYKRSAAL GQFVMHRGLI ISTMQAVFSS VFYFASVPLY 970 980 990 1000 1010 1020 QGFLMVGYAT IYTMFPVFSL VLDQDVKPEM AILYPELYKD LTKGRSLSFK TFLIWVLISI 1030 1040 1050 1060 1070 1080 YQGGILMYGA LLLFEDEFVH VVAISFTALI LTELLMVALT IRTWHWLMVV AEFLSLGCYV 1090 1100 1110 1120 1130 ASLAFLNEYF DVAFITTVTF LWKVSAITVV SCLPLYVLKY LKRKLSPPSY SKLSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 709-1146 (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[2]	"Differential expression of putative transbilayer amphipath transporters." Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P., Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D., Williamson P.L., Schlegel R.A. Physiol. Genomics 1:139-150(1999) [PubMed: 11015572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 52-1095 (ISOFORM 1). Strain: ICR. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC003246 mRNA. Translation: AAH03246.1. BC079626 mRNA. Translation: AAH79626.1. AF155913 mRNA. Translation: AAF08476.1.
IPI	IPI00471005. IPI00475210.
RefSeq	NP_001188498.1. NM_001201569.1. NP_056620.2. NM_015805.3.
UniGene	Mm.247138.
3D structure databases
ProteinModelPortal	P98195.
SMR	P98195. Positions 845-909.
ModBase	Search...
Protein-protein interaction databases
STRING	P98195.
PTM databases
PhosphoSite	P98195.
Proteomic databases
PRIDE	P98195.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025458; ENSMUSP00000025458; ENSMUSG00000024566.
GeneID	50771.
KEGG	mmu:50771.
UCSC	uc008fti.2. mouse.
Organism-specific databases
CTD	374868.
MGI	MGI:1354757. Atp9b.
Phylogenomic databases
eggNOG	roNOG05302.
GeneTree	ENSGT00550000074723.
HOGENOM	HBG720821.
HOVERGEN	HBG050617.
InParanoid	P98195.
Gene expression databases
ArrayExpress	P98195.
Bgee	P98195.
CleanEx	MM_ATP9B.
Genevestigator	P98195.
GermOnline	ENSMUSG00000024566. Mus musculus.
Family and domain databases
InterPro	IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 2 hits. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K01530.
Pfam	PF00122. E1-E2_ATPase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

ATP9B_MOUSE

Accession

Primary (citable) accession number: P98195
Secondary accession number(s): Q68FM3, Q99LI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 16, 2008
Last modified:	December 14, 2011
This is version 97 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program