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Protein

Probable phospholipid-transporting ATPase IIB

Gene

Atp9b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei467 – 46714-aspartylphosphate intermediateBy similarity
Metal bindingi873 – 8731MagnesiumBy similarity
Metal bindingi877 – 8771MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase IIB (EC:3.6.3.1)
Alternative name(s):
ATPase class II type 9B
Gene namesi
Name:Atp9b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1354757. Atp9b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 143143CytoplasmicSequence analysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence analysisAdd
BLAST
Topological domaini165 – 1728ExtracellularSequence analysis
Transmembranei173 – 19321HelicalSequence analysisAdd
BLAST
Topological domaini194 – 381188CytoplasmicSequence analysisAdd
BLAST
Transmembranei382 – 40221HelicalSequence analysisAdd
BLAST
Topological domaini403 – 4075ExtracellularSequence analysis
Transmembranei408 – 42720HelicalSequence analysisAdd
BLAST
Topological domaini428 – 938511CytoplasmicSequence analysisAdd
BLAST
Transmembranei939 – 95921HelicalSequence analysisAdd
BLAST
Topological domaini960 – 9612ExtracellularSequence analysis
Transmembranei962 – 98221HelicalSequence analysisAdd
BLAST
Topological domaini983 – 101129CytoplasmicSequence analysisAdd
BLAST
Transmembranei1012 – 103221HelicalSequence analysisAdd
BLAST
Topological domaini1033 – 10408ExtracellularSequence analysis
Transmembranei1041 – 106121HelicalSequence analysisAdd
BLAST
Topological domaini1062 – 10654CytoplasmicSequence analysis
Transmembranei1066 – 108621HelicalSequence analysisAdd
BLAST
Topological domaini1087 – 110519ExtracellularSequence analysisAdd
BLAST
Transmembranei1106 – 112823HelicalSequence analysisAdd
BLAST
Topological domaini1129 – 114618CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11461146Probable phospholipid-transporting ATPase IIBPRO_0000046378Add
BLAST

Proteomic databases

EPDiP98195.
MaxQBiP98195.
PaxDbiP98195.
PRIDEiP98195.

PTM databases

iPTMnetiP98195.
PhosphoSiteiP98195.
SwissPalmiP98195.

Expressioni

Tissue specificityi

Found in most tissues except spleen and muscle. Most abundant in testis. Also detected in fetal tissues.

Gene expression databases

CleanExiMM_ATP9B.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089394.

Structurei

3D structure databases

ProteinModelPortaliP98195.
SMRiP98195. Positions 706-984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0210. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000201571.
HOVERGENiHBG050617.
InParanoidiP98195.
KOiK01530.
PhylomeDBiP98195.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR030355. ATP9B.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF50. PTHR24092:SF50. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P98195-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADQIPLYPV RSAGAAASHR RAAYYSSAGP GPGADRRGRY QLEDESAHLD
60 70 80 90 100
EMPLMMSEEG FENDESDYHT LPRARITRRK RGLEWFVCGG WKFLCTSCCD
110 120 130 140 150
WLINVCQRKK ELKARTVWLG CPEKCEEKHP RNSIKNQKYN VFTFIPGVLY
160 170 180 190 200
EQFKFFLNLY FLVVSCSQFV PALKIGYLYT YWAPLGFVLA VTIAREAIDE
210 220 230 240 250
FRRFQRDKEM NSQLYSKLTV RGKVQVKSSD IQVGDLIIVE KNQRIPSDMV
260 270 280 290 300
FLRTSEKAGS CFIRTDQLDG ETDWKLKVAV SCTQRLPALG DLFSISAYVY
310 320 330 340 350
AQKPQLDIHS FEGTFTREDS DPPIHESLSI ENTLWASTIV ASGTVIGVVI
360 370 380 390 400
YTGKETRSVM NTSNPNNKVG LLDLELNQLT KALFLALVVL SVVMVTLQGF
410 420 430 440 450
AGPWYRNLFR FLLLFSYIIP ISLRVNLDMG KAAYGWMIMK DENIPGTVVR
460 470 480 490 500
TSTIPEELGR LVYLLTDKTG TLTQNEMVFK RLHLGTVSYG TDTMDEIQSH
510 520 530 540 550
VLNSYLQVHS QPSGHNPSSA PLRRSQSSTP KVKKSVSSRI HEAVKAIALC
560 570 580 590 600
HNVTPVYEAR AGITGETEFA EADQDFSDEN RTYQASSPDE VALVRWTESV
610 620 630 640 650
GLTLVSRDLA SMQLKTPSGQ VLTYCILQMF PFTSESKRMG IIVRDESTAE
660 670 680 690 700
ITFYMKGADV AMSTIVQYND WLEEECGNMA REGLRTLVVA KRTLTEEQYQ
710 720 730 740 750
DFESRYSQAK LSIHDRALKV AAVVESLERE MELLCLTGVE DQLQADVRPT
760 770 780 790 800
LEMLRNAGIK IWMLTGDKLE TATCIAKSSH LVSRTQDIHV FRPVTSRGEA
810 820 830 840 850
HLELNAFRRK HDCALVISGD SLEVCLRYYE HELVELACQC PAVVCCRCSP
860 870 880 890 900
TQKAHIVTLL RQHTRKRTCA IGDGGNDVSM IQAADCGIGI EGKEGKQASL
910 920 930 940 950
AADFSITQFR HIGRLLMVHG RNSYKRSAAL GQFVMHRGLI ISTMQAVFSS
960 970 980 990 1000
VFYFASVPLY QGFLMVGYAT IYTMFPVFSL VLDQDVKPEM AILYPELYKD
1010 1020 1030 1040 1050
LTKGRSLSFK TFLIWVLISI YQGGILMYGA LLLFEDEFVH VVAISFTALI
1060 1070 1080 1090 1100
LTELLMVALT IRTWHWLMVV AEFLSLGCYV ASLAFLNEYF GIGRVSFGAF
1110 1120 1130 1140
LDVAFITTVT FLWKVSAITV VSCLPLYVLK YLKRKLSPPS YSKLSS
Length:1,146
Mass (Da):129,017
Last modified:December 16, 2008 - v4
Checksum:i334AAEE291DFDC2E
GO
Isoform 2 (identifier: P98195-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1091-1101: Missing.

Note: No experimental confirmation available.
Show »
Length:1,135
Mass (Da):127,912
Checksum:iFF41C197C7AA7D58
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti366 – 3661N → K in AAH79626 (PubMed:15489334).Curated
Sequence conflicti605 – 6051V → I in AAH79626 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1091 – 110111Missing in isoform 2. 1 PublicationVSP_035908Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003246 mRNA. Translation: AAH03246.1.
BC079626 mRNA. Translation: AAH79626.1.
AF155913 mRNA. Translation: AAF08476.1.
CCDSiCCDS29370.1. [P98195-2]
RefSeqiNP_001188498.1. NM_001201569.1.
NP_056620.2. NM_015805.3.
UniGeneiMm.247138.

Genome annotation databases

GeneIDi50771.
KEGGimmu:50771.
UCSCiuc008fti.2. mouse. [P98195-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003246 mRNA. Translation: AAH03246.1.
BC079626 mRNA. Translation: AAH79626.1.
AF155913 mRNA. Translation: AAF08476.1.
CCDSiCCDS29370.1. [P98195-2]
RefSeqiNP_001188498.1. NM_001201569.1.
NP_056620.2. NM_015805.3.
UniGeneiMm.247138.

3D structure databases

ProteinModelPortaliP98195.
SMRiP98195. Positions 706-984.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089394.

PTM databases

iPTMnetiP98195.
PhosphoSiteiP98195.
SwissPalmiP98195.

Proteomic databases

EPDiP98195.
MaxQBiP98195.
PaxDbiP98195.
PRIDEiP98195.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi50771.
KEGGimmu:50771.
UCSCiuc008fti.2. mouse. [P98195-1]

Organism-specific databases

CTDi374868.
MGIiMGI:1354757. Atp9b.

Phylogenomic databases

eggNOGiKOG0210. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000201571.
HOVERGENiHBG050617.
InParanoidiP98195.
KOiK01530.
PhylomeDBiP98195.

Miscellaneous databases

PROiP98195.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ATP9B.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR030355. ATP9B.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF50. PTHR24092:SF50. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 709-1146 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Differential expression of putative transbilayer amphipath transporters."
    Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P., Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D., Williamson P.L., Schlegel R.A.
    Physiol. Genomics 1:139-150(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 52-1095 (ISOFORM 1).
    Strain: ICR.
    Tissue: Brain.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiATP9B_MOUSE
AccessioniPrimary (citable) accession number: P98195
Secondary accession number(s): Q68FM3, Q99LI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 16, 2008
Last modified: July 6, 2016
This is version 134 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.