P98192 (GNPAT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxyacetone phosphate acyltransferase
Short name=DAP-AT
Short name=DHAP-AT
EC=2.3.1.42

Alternative name(s):
Acyl-CoA:dihydroxyacetonephosphateacyltransferase
Glycerone-phosphate O-acyltransferase

Gene names

Name:	Gnpat
Synonyms:	Dhapat

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism.
Subunit structure	May be part of an heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT.
Subcellular location	Peroxisome membrane; Peripheral membrane protein; Matrix side By similarity. Note: Exclusively localized to the lumenal side of the peroxisomal membrane By similarity.
Tissue specificity	Highly expressed in liver and testis. Lower levels in heart, brain, lung and kidney. Detected in spleen.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Cellular component	Membrane Peroxisome
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular membrane organization Inferred from mutant phenotype. Source: MGI cerebellum morphogenesis Inferred from mutant phenotype. Source: MGI ether lipid biosynthetic process Inferred from mutant phenotype. Source: MGI paranodal junction assembly Inferred from mutant phenotype. Source: MGI phospholipid biosynthetic process Inferred from electronic annotation. Source: InterPro synapse assembly Inferred from mutant phenotype. Source: MGI
Cellular component	peroxisomal matrix Traceable author statement Ref.1. Source: MGI peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: InterPro glycerone-phosphate O-acyltransferase activity Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Dihydroxyacetone phosphate acyltransferase		PRO_0000195247
Regions
	Motif	161 – 166	6	HXXXXD motif
	Motif	676 – 678	3	Microbody targeting signal Potential
	Compositional bias	5 – 9	5	Poly-Ser
Amino acid modifications
	Modified residue	641	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P98192 [UniParc]. Last modified May 30, 2000. Version 1. Checksum: FA7245A2DDD174FF	FASTA	678	76,870
	10 20 30 40 50 60 MDVPSSSSSR FSVGSASPSS VLLYAKDLKK WDEFEDLLEE RRHISDFKFA MKCYTPPLYR 70 80 90 100 110 120 GITPCKPGDI KSIVLSSEEI NYVIKQLSRE SLTGVDVLRE EASEILEEMS HKLRIGAIRF 130 140 150 160 170 180 FAFVLSKIFK QIFSKVCVNE EGIQKLQRAV QEHPVVLLPS HRSYIDFLML SFILYSYDLP 190 200 210 220 230 240 VPVIAAGMDF LGMRVVSELL RMSGAFFMRR TFGGNKLYWA VFSEYVKTML RCGYAPVEFF 250 260 270 280 290 300 LEGTRSRAAK TLTPKFGLLN IVMEPFFKRE VFDTYFVPIS ISYDKILEES LYAYEILGVP 310 320 330 340 350 360 KPKESTTGLL KARRILSENF GSIHVYFGDP VSLRSLAAGR LNRNTYNLVP RCIPQKQPED 370 380 390 400 410 420 VQAFVTEVAY KMQLLQIENL ALSPWLLVVT ILLQNQLSMD FDALVEKTLW LKGVTQVFGG 430 440 450 460 470 480 FLLWPDNKLP EEVVQSSILL HSNLASLVKD QVVLKMNSGS SQVVNGLVPE HIALLMCSAY 490 500 510 520 530 540 RNQLLNIFAR PSLVALALHM TPGLRKEDVF SCFSFLRNVF SDEFIFLPGN TLRDFEEGCY 550 560 570 580 590 600 LLCKAEAMQM AGKDIILTDK GTAVLQFLTS LFKPFVESYQ LLCRYLLHEE DYFGEKEYLV 610 620 630 640 650 660 AARKFTRQLL DQGSSQCYDA LSSELQKNAL AAFVRLGVVE KKKVDSKYVY YVNGPATSKL 670 EEMLGCKKPI GKPATAKL

References

[1]	"Identification and characterization of the mouse cDNA encoding acyl-CoA:dihydroxyacetone phosphate acyltransferase." Ofman R., Hogenhout E.M., Wanders R.J.A. Biochim. Biophys. Acta 1439:89-94(1999) [PubMed: 10395968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Fibroblast.
[2]	"Synthesis of plasmalogens in eye lens epithelial cells." Thai T.P., Rodemer C., Worsch J., Hunziker A., Gorgas K., Just W.W. FEBS Lett. 456:263-268(1999) [PubMed: 10456321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF110769 mRNA. Translation: AAD55351.1. AJ132012 mRNA. Translation: CAB41975.1.
IPI	IPI00126660.
RefSeq	NP_034452.3. NM_010322.3.
UniGene	Mm.29114.
3D structure databases
ProteinModelPortal	P98192.
ModBase	Search...
Protein-protein interaction databases
IntAct	P98192. 2 interactions.
STRING	P98192.
PTM databases
PhosphoSite	P98192.
Proteomic databases
PRIDE	P98192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034466; ENSMUSP00000034466; ENSMUSG00000031985.
GeneID	14712.
KEGG	mmu:14712.
Organism-specific databases
CTD	8443.
MGI	MGI:1343460. Gnpat.
Phylogenomic databases
eggNOG	roNOG08043.
HOGENOM	HBG445195.
HOVERGEN	HBG051749.
InParanoid	P98192.
OMA	PRYIPQK.
OrthoDB	EOG4DJJW5.
PhylomeDB	P98192.
Gene expression databases
ArrayExpress	P98192.
Bgee	P98192.
CleanEx	MM_GNPAT.
Genevestigator	P98192.
GermOnline	ENSMUSG00000031985. Mus musculus.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00649.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286715.
SOURCE	Search...

Entry information

Entry name

GNPAT_MOUSE

Accession

Primary (citable) accession number: P98192
Secondary accession number(s): Q9WUT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program