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Protein

Dihydroxyacetone phosphate acyltransferase

Gene

Gnpat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

GO - Molecular functioni

  • catalytic activity Source: MGI
  • glycerone-phosphate O-acyltransferase activity Source: MGI
  • palmitoyl-CoA hydrolase activity Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

  • cerebellum morphogenesis Source: MGI
  • ether lipid biosynthetic process Source: MGI
  • glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  • membrane organization Source: MGI
  • myelination Source: MGI
  • paranodal junction assembly Source: MGI
  • response to drug Source: Ensembl
  • response to fatty acid Source: Ensembl
  • response to nutrient Source: Ensembl
  • response to starvation Source: Ensembl
  • synapse assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-1483166. Synthesis of PA.
R-MMU-75896. Plasmalogen biosynthesis.
UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxyacetone phosphate acyltransferase (EC:2.3.1.42)
Short name:
DAP-AT
Short name:
DHAP-AT
Alternative name(s):
Acyl-CoA:dihydroxyacetonephosphateacyltransferase
Glycerone-phosphate O-acyltransferase
Gene namesi
Name:Gnpat
Synonyms:Dhapat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1343460. Gnpat.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: MGI
  • mitochondrion Source: Ensembl
  • peroxisomal matrix Source: MGI
  • peroxisomal membrane Source: MGI
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 678678Dihydroxyacetone phosphate acyltransferasePRO_0000195247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121PhosphoserineCombined sources
Modified residuei17 – 171PhosphoserineBy similarity
Modified residuei641 – 6411N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP98192.
PaxDbiP98192.
PeptideAtlasiP98192.
PRIDEiP98192.

PTM databases

iPTMnetiP98192.
PhosphoSiteiP98192.

Expressioni

Tissue specificityi

Highly expressed in liver and testis. Lower levels in heart, brain, lung and kidney. Detected in spleen.

Gene expression databases

BgeeiENSMUSG00000031985.
CleanExiMM_GNPAT.
ExpressionAtlasiP98192. baseline and differential.
GenevisibleiP98192. MM.

Interactioni

Subunit structurei

May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT.

GO - Molecular functioni

Protein-protein interaction databases

IntActiP98192. 2 interactions.
MINTiMINT-1713130.
STRINGi10090.ENSMUSP00000034466.

Structurei

3D structure databases

ProteinModelPortaliP98192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi161 – 1666HXXXXD motif
Motifi676 – 6783Microbody targeting signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 95Poly-Ser

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.Curated

Phylogenomic databases

eggNOGiKOG3730. Eukaryota.
COG2937. LUCA.
HOGENOMiHOG000112751.
HOVERGENiHBG051749.
InParanoidiP98192.
KOiK00649.
OMAiPRYIPQK.
OrthoDBiEOG091G0378.
PhylomeDBiP98192.
TreeFamiTF313360.

Family and domain databases

InterProiIPR028353. DHAPAT.
IPR022284. GPAT/DHAPAT.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 1 hit.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500063. DHAPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P98192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVPSSSSSR FSVGSASPSS VLLYAKDLKK WDEFEDLLEE RRHISDFKFA
60 70 80 90 100
MKCYTPPLYR GITPCKPGDI KSIVLSSEEI NYVIKQLSRE SLTGVDVLRE
110 120 130 140 150
EASEILEEMS HKLRIGAIRF FAFVLSKIFK QIFSKVCVNE EGIQKLQRAV
160 170 180 190 200
QEHPVVLLPS HRSYIDFLML SFILYSYDLP VPVIAAGMDF LGMRVVSELL
210 220 230 240 250
RMSGAFFMRR TFGGNKLYWA VFSEYVKTML RCGYAPVEFF LEGTRSRAAK
260 270 280 290 300
TLTPKFGLLN IVMEPFFKRE VFDTYFVPIS ISYDKILEES LYAYEILGVP
310 320 330 340 350
KPKESTTGLL KARRILSENF GSIHVYFGDP VSLRSLAAGR LNRNTYNLVP
360 370 380 390 400
RCIPQKQPED VQAFVTEVAY KMQLLQIENL ALSPWLLVVT ILLQNQLSMD
410 420 430 440 450
FDALVEKTLW LKGVTQVFGG FLLWPDNKLP EEVVQSSILL HSNLASLVKD
460 470 480 490 500
QVVLKMNSGS SQVVNGLVPE HIALLMCSAY RNQLLNIFAR PSLVALALHM
510 520 530 540 550
TPGLRKEDVF SCFSFLRNVF SDEFIFLPGN TLRDFEEGCY LLCKAEAMQM
560 570 580 590 600
AGKDIILTDK GTAVLQFLTS LFKPFVESYQ LLCRYLLHEE DYFGEKEYLV
610 620 630 640 650
AARKFTRQLL DQGSSQCYDA LSSELQKNAL AAFVRLGVVE KKKVDSKYVY
660 670
YVNGPATSKL EEMLGCKKPI GKPATAKL
Length:678
Mass (Da):76,870
Last modified:May 30, 2000 - v1
Checksum:iFA7245A2DDD174FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110769 mRNA. Translation: AAD55351.1.
AJ132012 mRNA. Translation: CAB41975.1.
CCDSiCCDS22776.1.
RefSeqiNP_034452.3. NM_010322.3.
UniGeneiMm.29114.

Genome annotation databases

EnsembliENSMUST00000034466; ENSMUSP00000034466; ENSMUSG00000031985.
GeneIDi14712.
KEGGimmu:14712.
UCSCiuc009nxv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110769 mRNA. Translation: AAD55351.1.
AJ132012 mRNA. Translation: CAB41975.1.
CCDSiCCDS22776.1.
RefSeqiNP_034452.3. NM_010322.3.
UniGeneiMm.29114.

3D structure databases

ProteinModelPortaliP98192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP98192. 2 interactions.
MINTiMINT-1713130.
STRINGi10090.ENSMUSP00000034466.

PTM databases

iPTMnetiP98192.
PhosphoSiteiP98192.

Proteomic databases

EPDiP98192.
PaxDbiP98192.
PeptideAtlasiP98192.
PRIDEiP98192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034466; ENSMUSP00000034466; ENSMUSG00000031985.
GeneIDi14712.
KEGGimmu:14712.
UCSCiuc009nxv.2. mouse.

Organism-specific databases

CTDi8443.
MGIiMGI:1343460. Gnpat.

Phylogenomic databases

eggNOGiKOG3730. Eukaryota.
COG2937. LUCA.
HOGENOMiHOG000112751.
HOVERGENiHBG051749.
InParanoidiP98192.
KOiK00649.
OMAiPRYIPQK.
OrthoDBiEOG091G0378.
PhylomeDBiP98192.
TreeFamiTF313360.

Enzyme and pathway databases

UniPathwayiUPA00940.
ReactomeiR-MMU-1483166. Synthesis of PA.
R-MMU-75896. Plasmalogen biosynthesis.

Miscellaneous databases

ChiTaRSiGnpat. mouse.
PROiP98192.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031985.
CleanExiMM_GNPAT.
ExpressionAtlasiP98192. baseline and differential.
GenevisibleiP98192. MM.

Family and domain databases

InterProiIPR028353. DHAPAT.
IPR022284. GPAT/DHAPAT.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 1 hit.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500063. DHAPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGNPAT_MOUSE
AccessioniPrimary (citable) accession number: P98192
Secondary accession number(s): Q9WUT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.