Reviewed, UniProtKB/Swiss-Prot P98185 (RIP1_SAPOF)

Names and origin

Protein names

Recommended name:
    Ribosome-inactivating protein saporin-1
      Short name=SAP-1
    EC=3.2.2.22
Alternative name(s):
    SO-4
    rRNA N-glycosidase

Gene names

Name:

SAP1

Organism

Saponaria officinalis (Common soapwort)

Taxonomic identifier

3572 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Saponaria

Protein attributes

Sequence length	40 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells By similarity.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›40	›40	Ribosome-inactivating protein saporin-1		PRO_0000221411
Experimental info
	Non-terminal residue	40	1

Sequences

	Sequence		Length	Mass (Da)
	P98185-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F4615857A9871BF2	FASTA	40	4,625
	10 20 30 40 VIIYELNLQG TTKAQYSTIL KQLRDDIKDP NLXYGXXDYS

References

[1]	"N-terminal sequence of some ribosome-inactivating proteins." Montecucchi P.-C., Lazzarini A.M., Barbieri L., Stirpe F., Soria M., Lappi D. Int. J. Pept. Protein Res. 33:263-267(1989) [PubMed: 2753596] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Leaf.

Cross-references

Sequence databases
PIR	S16331.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 39707.
Family and domain databases
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP1_SAPOF

Accession

Primary (citable) accession number: P98185

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)