Reviewed, UniProtKB/Swiss-Prot P98184 (RIP2_BRYDI)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein bryodin II     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase     BD2
Organism	Bryonia dioica (Red bryony)
Taxonomic identifier	3652 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Bryonia

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells By similarity.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2
	Chain	22 – 282	261	Ribosome-inactivating protein bryodin II		PRO_0000030756
Sites
	Active site	183	1	By similarity
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P98184-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C52BE2F6A873769C	FASTA	282	30,754
	10 20 30 40 50 60 MRSIGFYSVL ALYVGAHVTE DVDINFSLIG ATGATYKTFI RNLRTKLTVG TPRVYDIPVL 70 80 90 100 110 120 RNAAAGLARF QLVTLTNYNG ESVTVALDVV NVYVVAYRAG NTAYFLADAS TEANNVLFAG 130 140 150 160 170 180 INHVRLPYGG NYDGLETAAG RISRENIELG FSEISSAIGN MFRHNPGTSV PRAFIVIIQT 190 200 210 220 230 240 VSEAARFKYI EQRVSENVGT KFKPDPAFLS LQNAWGSLSE QIQIAQTRGG EFARPVELRT 250 260 270 280 VSNTPTFVTN VNSPVVKGIA LLLYFRVNVG TDNVFAMSLS TY

References

[1]	"Bryodin 2 a ribosome-inactivating protein isolated from the plant Bryonia dioica." Siegall C.B., Gawlak S.L., Marquardt H. Patent number US5597569, 28-JAN-1997 Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Characterization of ribosome-inactivating proteins isolated from Bryonia dioica and their utility as carcinoma-reactive immunoconjugates." Siegall C.B., Gawlak S.L., Chace D., Wolff E.A., Mixan B., Marquardt H. Bioconj. Chem. 5:423-429(1994) [PubMed: 7849072] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-42. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	I34238 Unassigned DNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1CF5 based on UniProtKB P29339.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 256122.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP2_BRYDI

Accession

Primary (citable) accession number: P98184
Secondary accession number(s): Q9S8J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)