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Protein

Tabersonine 16-hydroxylase 1

Gene

CYP71D12

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the flower biosynthesis of vindoline, a precursor of vinblastine and vincristine (PubMed:10481044, PubMed:12228585, PubMed:24108213). Hydroxylates specifically tabersonine, 2,3-dihydrotabersonine and 2,3-dihydro-3-hydroxytabersonine, but has no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline and catharanthine (PubMed:24108213).3 Publications

Miscellaneous

Tabersonine 16-hydroxylation is orchestrated in an organ-dependent manner by two genes including CYP71D351, which encodes the T16H2 isoform acting in the foliar vindoline biosynthesis, and CYP71D12, which encodes the T16H1 isoform acting in the flower vindoline biosynthesis.1 Publication

Catalytic activityi

Tabersonine + NADPH + O2 = 16-hydroxytabersonine + NADP+ + H2O.2 Publications

Cofactori

hemeBy similarity

Kineticsi

concentrations of tabersonine greater than 30 µM are inhibitory.1 Publication
  1. KM=11 µM for tabersonine in a T16H/16OMT coupled system1 Publication
  2. KM=350 nM for tabersonine1 Publication
  3. KM=14 µM for NADPH in a T16H/16OMT coupled system1 Publication

    pH dependencei

    Optimum pH is 7.5 in phosphate buffer and 8.0 in TRIS-HCl.1 Publication

    Temperature dependencei

    Stable at 30 degrees Celsius.1 Publication

    Pathwayi: vindoline biosynthesis

    This protein is involved in the pathway vindoline biosynthesis, which is part of Alkaloid biosynthesis.Curated
    View all proteins of this organism that are known to be involved in the pathway vindoline biosynthesis and in Alkaloid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi447Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    Biological processAlkaloid metabolism
    LigandHeme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-7709.
    BRENDAi1.14.13.73. 1211.
    SABIO-RKiP98183.
    UniPathwayiUPA00365.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tabersonine 16-hydroxylase 11 Publication (EC:1.14.13.732 Publications)
    Alternative name(s):
    Cytochrome P450 71D121 Publication
    Gene namesi
    Name:CYP71D121 Publication
    Synonyms:T16H11 Publication
    OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
    Taxonomic identifieri4058 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003107281 – 506Tabersonine 16-hydroxylase 1Add BLAST506

    Expressioni

    Tissue specificityi

    Predominantly expressed in young leaves of mature plants (PubMed:12228585, PubMed:16262708). Low expression in roots and flowers, but not detected in stems and old leaves (PubMed:12228585, PubMed:16262708). Found predominantly in leaf epidermis (PubMed:12228585, PubMed:16262708). Barely detected in roots, internodes, young and mature leaves, and flower buds, but relatively abundant in fully developped flowers (PubMed:24108213). Not detected in leaf epidermal cells (PubMed:24108213).3 Publications

    Developmental stagei

    Peak of expression after light treatment at 9 days of seedling development.1 Publication

    Inductioni

    Up-regulated by methyl jasmonate (PubMed:24108213). Inhibited by cytochrome c, CO, clotrimazole, miconazole, tricliphane, flusilazole and tetcyclasis, and with a lower efficiency, by 1-phenylimidazole and piperonylbutoxide. Not inhibited by potassium cyanide and sodium azide.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliP98183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi501 – 504Poly-SerSequence analysis4

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK12696.

    Family and domain databases

    InterProiView protein in InterPro
    IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    PfamiView protein in Pfam
    PF00067. p450. 1 hit.
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiView protein in PROSITE
    PS00086. CYTOCHROME_P450. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P98183-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEFYYFLYLA FLLFCFILSK TTKKFGQNSQ YSNHDELPPG PPQIPILGNA
    60 70 80 90 100
    HQLSGGHTHH ILRDLAKKYG PLMHLKIGEV STIVASSPQI AEEIFRTHDI
    110 120 130 140 150
    LFADRPSNLE SFKIVSYDFS DMVVSPYGNY WRQLRKISMM ELLSQKSVQS
    160 170 180 190 200
    FRSIREEEVL NFIKSIGSKE GTRINLSKEI SLLIYGITTR AAFGEKNKNT
    210 220 230 240 250
    EEFIRLLDQL TKAVAEPNIA DMFPSLKFLQ LISTSKYKIE KIHKQFDVIV
    260 270 280 290 300
    ETILKGHKEK INKPLSQENG EKKEDLVDVL LNIQRRNDFE APLGDKNIKA
    310 320 330 340 350
    IIFNIFSAGT ETSSTTVDWA MCEMIKNPTV MKKAQEEVRK VFNEEGNVDE
    360 370 380 390 400
    TKLHQLKYLQ AVIKETLRLH PPVPLLLPRE CREQCKIKGY TIPSKSRVIV
    410 420 430 440 450
    NAWAIGRDPN YWIEPEKFNP DRFLESKVDF KGNSFEYLPF GGGRRICPGI
    460 470 480 490 500
    TFALANIELP LAQLLFHFDW QSNTEKLNMK ESRGVTVRRE DDLYLTPVNF

    SSSSPA
    Length:506
    Mass (Da):58,079
    Last modified:May 10, 2017 - v2
    Checksum:i485FF14E6D34901B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FJ647194 mRNA. Translation: ACM92061.1.
    AJ238612 mRNA. Translation: CAB56503.1.

    Genome annotation databases

    KEGGiag:CAB56503.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiC71DC_CATRO
    AccessioniPrimary (citable) accession number: P98183
    Secondary accession number(s): C0KYN4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: May 10, 2017
    Last modified: July 5, 2017
    This is version 74 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families