Reviewed, UniProtKB/Swiss-Prot P98183 (C71DC_CATRO)

Names and origin

Protein names

Recommended name:
    Tabersonine 16-hydroxylase
    EC=1.14.13.73
Alternative name(s):
    Cytochrome P450 71D12

Gene names

Name:

CYP71D12

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	495 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of vindoline, a precursor of vinblastine and vincristine.
Catalytic activity	Tabersonine + NADPH + O(2) = 16-hydroxytabersonine + NADP(+) + H(2)O.
Cofactor	Heme group By similarity.
Pathway	Alkaloid biosynthesis; vindoline biosynthesis; vindoline from tabersonine: step 1/6.
Subcellular location	Endoplasmic reticulum.
Tissue specificity	Predominantly expressed in young leaves of mature plants. Low expression in roots and flowers, but not detected in stems and old leaves. Found predominantly in leaf epidermis.
Developmental stage	Peak of expression after light treatment at 9 days of seedling development.
Induction	Inhibited by cytochrome c, CO, clotrimazole, miconazole, tricliphane, flusilazole and tetcyclasis, and with a lower efficiency, by 1-phenylimidazole and piperonylbutoxide. Not inhibited by potassium cyanide and sodium azide.
Sequence similarities	Belongs to the cytochrome P450 family.
Biophysicochemical properties	Kinetic parameters: concentrations of tabersonine greater than 30 µM are inhibitory. KM=11 µM for tabersonine KM=14 µM for NADPH pH dependence: Optimum pH is 7.5 in phosphate buffer and 8.0 in TRIS-HCl. Temperature dependence: Stable at 30 degrees Celsius.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro tabersonine 16-hydroxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 495	›495	Tabersonine 16-hydroxylase		PRO_0000310728
Regions
	Compositional bias	490 – 493	4	Poly-Ser
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P98183-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D769003236430161	FASTA	495	56,590
	10 20 30 40 50 60 LLFCFILSKT TKKFGQNSQY SNHDELPPGP PQIPILGNAH QLSGGHTHHI LRDLAKKYGP 70 80 90 100 110 120 LMHLKIGEVS TIVASSPQIA EEIFRTHDIL FADRPSNLES FKIVSYDFSD MVVSPYGNYW 130 140 150 160 170 180 RQLRKISMME LLSQKSVQSF RSIREEEVLN FIKSIGSKEG TRINLSKEIS LLIYGITTRA 190 200 210 220 230 240 AFGEKNKNTE EFIRLLDQLT KAVAEPNIAD MFPSLKFLQL ISTSKYKIEK IHKQFDVIVE 250 260 270 280 290 300 TILKGHKEKI NKPLSQENGE KKEDLVDVLL NIQRRNDFEA PLGDKNIKAI IFNIFSAGTE 310 320 330 340 350 360 TSSTTVDWAM CEMIKNPTVM KKAQEEVRKV FNEEGNVDET KLHQLKYLQA VIKETLRLHP 370 380 390 400 410 420 PVPLLLPREC REQCKIKGYT IPSKSRVIVN AWAIGRDPNY WIEPEKFNPD RFLESKVDFK 430 440 450 460 470 480 GNSFEYLPFG GGRRICPGIT FALANIELPL AQLLFHFDWQ SNTEKLNMKE SRGVTVRRED 490 DLYLTPVNFS SSSPA

References

[1]	"Light-induced cytochrome P450-dependent enzyme in indole alkaloid biosynthesis: tabersonine 16-hydroxylase." Schroeder G., Unterbusch E., Kaltenbach M., Schmidt J., Strack D., Schroeder J. FEBS Lett. 458:97-102(1999) [PubMed: 10481044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A cytochrome P-450 monooxygenase catalyzes the first step in the conversion of tabersonine to vindoline in Catharanthus roseus." St Pierre B., De Luca V. Plant Physiol. 109:131-139(1995) [PubMed: 12228585] [Abstract] Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"Localization of tabersonine 16-hydroxylase and 16-OH tabersonine-16-O-methyltransferase to leaf epidermal cells defines them as a major site of precursor biosynthesis in the vindoline pathway in Catharanthus roseus." Murata J., Luca V.D. Plant J. 44:581-594(2005) [PubMed: 16262708] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ238612 mRNA. Translation: CAB56503.1.
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-7709.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C71DC_CATRO

Accession

Primary (citable) accession number: P98183

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 1, 2000
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)