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Protein

Tabersonine 16-hydroxylase

Gene

CYP71D12

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of vindoline, a precursor of vinblastine and vincristine.

Catalytic activityi

Tabersonine + NADPH + O2 = 16-hydroxytabersonine + NADP+ + H2O.

Cofactori

hemeBy similarity

Kineticsi

concentrations of tabersonine greater than 30 µM are inhibitory.

  1. KM=11 µM for tabersonine
  2. KM=14 µM for NADPH

    pH dependencei

    Optimum pH is 7.5 in phosphate buffer and 8.0 in TRIS-HCl.

    Temperature dependencei

    Stable at 30 degrees Celsius.

    Pathwayi: vindoline biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes vindoline from tabersonine.
    Proteins known to be involved in the 6 steps of the subpathway in this organism are:
    1. Tabersonine 16-hydroxylase (CYP71D12)
    2. no protein annotated in this organism
    3. no protein annotated in this organism
    4. no protein annotated in this organism
    5. Deacetoxyvindoline 4-hydroxylase (D4H)
    6. Deacetylvindoline O-acetyltransferase (DAT)
    This subpathway is part of the pathway vindoline biosynthesis, which is itself part of Alkaloid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes vindoline from tabersonine, the pathway vindoline biosynthesis and in Alkaloid biosynthesis.

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-7709.
    BRENDAi1.14.13.73. 1211.
    SABIO-RKP98183.
    UniPathwayiUPA00365; UER00521.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tabersonine 16-hydroxylase (EC:1.14.13.73)
    Alternative name(s):
    Cytochrome P450 71D12
    Gene namesi
    Name:CYP71D12
    OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
    Taxonomic identifieri4058 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

    Subcellular locationi

    • Endoplasmic reticulum 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 495›495Tabersonine 16-hydroxylasePRO_0000310728Add
    BLAST

    Expressioni

    Tissue specificityi

    Predominantly expressed in young leaves of mature plants. Low expression in roots and flowers, but not detected in stems and old leaves. Found predominantly in leaf epidermis.2 Publications

    Developmental stagei

    Peak of expression after light treatment at 9 days of seedling development.1 Publication

    Inductioni

    Inhibited by cytochrome c, CO, clotrimazole, miconazole, tricliphane, flusilazole and tetcyclasis, and with a lower efficiency, by 1-phenylimidazole and piperonylbutoxide. Not inhibited by potassium cyanide and sodium azide.

    Structurei

    3D structure databases

    ProteinModelPortaliP98183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi490 – 4934Poly-Ser

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    KOiK12696.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P98183-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    LLFCFILSKT TKKFGQNSQY SNHDELPPGP PQIPILGNAH QLSGGHTHHI
    60 70 80 90 100
    LRDLAKKYGP LMHLKIGEVS TIVASSPQIA EEIFRTHDIL FADRPSNLES
    110 120 130 140 150
    FKIVSYDFSD MVVSPYGNYW RQLRKISMME LLSQKSVQSF RSIREEEVLN
    160 170 180 190 200
    FIKSIGSKEG TRINLSKEIS LLIYGITTRA AFGEKNKNTE EFIRLLDQLT
    210 220 230 240 250
    KAVAEPNIAD MFPSLKFLQL ISTSKYKIEK IHKQFDVIVE TILKGHKEKI
    260 270 280 290 300
    NKPLSQENGE KKEDLVDVLL NIQRRNDFEA PLGDKNIKAI IFNIFSAGTE
    310 320 330 340 350
    TSSTTVDWAM CEMIKNPTVM KKAQEEVRKV FNEEGNVDET KLHQLKYLQA
    360 370 380 390 400
    VIKETLRLHP PVPLLLPREC REQCKIKGYT IPSKSRVIVN AWAIGRDPNY
    410 420 430 440 450
    WIEPEKFNPD RFLESKVDFK GNSFEYLPFG GGRRICPGIT FALANIELPL
    460 470 480 490
    AQLLFHFDWQ SNTEKLNMKE SRGVTVRRED DLYLTPVNFS SSSPA
    Length:495
    Mass (Da):56,590
    Last modified:May 1, 2000 - v1
    Checksum:iD769003236430161
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ238612 mRNA. Translation: CAB56503.1.

    Genome annotation databases

    KEGGiag:CAB56503.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ238612 mRNA. Translation: CAB56503.1.

    3D structure databases

    ProteinModelPortaliP98183.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:CAB56503.

    Phylogenomic databases

    KOiK12696.

    Enzyme and pathway databases

    UniPathwayiUPA00365; UER00521.
    BioCyciMetaCyc:MONOMER-7709.
    BRENDAi1.14.13.73. 1211.
    SABIO-RKP98183.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiC71DC_CATRO
    AccessioniPrimary (citable) accession number: P98183
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: May 1, 2000
    Last modified: November 11, 2015
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.