Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor p65

Gene

RELA

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-GGA-1227892. TRAF6 mediated NF-kB activation.
R-GGA-434001. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-GGA-434131. NFkB activation mediated by RIP1 complexed with activated TLR3.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor p65
Alternative name(s):
Nuclear factor NF-kappa-B p65 subunit
Gene namesi
Name:RELA
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002051711 – 558Transcription factor p65Add BLAST558

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei281Phosphoserine; by PKASequence analysis1

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Spleen; lower level in brain.

Interactioni

Subunit structurei

Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-c-Rel complex. Component of the NF-kappa-B p65-p105 complex.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP98152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 311RHDPROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi306 – 309Nuclear localization signalSequence analysis4

Sequence similaritiesi

Contains 1 RHD (Rel-like) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG017916.
InParanoidiP98152.
KOiK04735.
PhylomeDBiP98152.

Family and domain databases

CDDicd01177. IPT_NFkappaB. 1 hit.
Gene3Di2.60.40.10. 1 hit.
2.60.40.340. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR033926. IPT_NFkappaB.
IPR000451. NFkB/Dor.
IPR008967. p53-like_TF_DNA-bd.
IPR030495. RelA.
IPR030492. RHD_CS.
IPR032397. RHD_dimer.
IPR011539. RHD_DNA_bind_dom.
[Graphical view]
PANTHERiPTHR24169. PTHR24169. 2 hits.
PTHR24169:SF1. PTHR24169:SF1. 2 hits.
PfamiPF16179. RHD_dimer. 1 hit.
PF00554. RHD_DNA_bind. 1 hit.
[Graphical view]
PRINTSiPR00057. NFKBTNSCPFCT.
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS01204. REL_1. 1 hit.
PS50254. REL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P98152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPADLLPLY LQPEWGEQEP GGATPFVEIL EQPKQRGMRF RYKCEGRSAG
60 70 80 90 100
SIPGEHSTDS ARTHPTIRVN HYRGPGRVRV SLVTKDPPHG PHPHELVGRH
110 120 130 140 150
CQHGYYEAEL SPERCVHSFQ NLGIQCVKKR ELEAAVAERI RTNNNPFNVP
160 170 180 190 200
MEERGAEYDL SAVRLCFQVW VNGPGGLCPL PPVLSQPIYD NRAPSTAELR
210 220 230 240 250
ILPGDRNSGS CQGGDEIFLL CDKVQKEDIE VRFWAEGWEA KGSFAAADVH
260 270 280 290 300
RQVAIVFRTP PFRERSLRHP VTVRMELQRP SDRQRSPPLD FRYLPHQGDL
310 320 330 340 350
QCIEEKRKRT RDTFRAFVQR APLPGLEPNP EPRPPRRIAV PSRPPPAPQQ
360 370 380 390 400
PPSMVGAPPA PLFPLGVPPA SSPTPEPLAE ALLQLQFDDG VGGSGPPPST
410 420 430 440 450
TTTTTTTQCA LGGGIPDPGG SPLDLGALLG DPPFDTIDAA ELQRLLGPPE
460 470 480 490 500
TPPGGIGAGG GFGELLSLPT NFGDPPSSTA ATFGPSPPML LSYPEAITRL
510 520 530 540 550
VQCQTPGGSG GGGPPVGPPQ DLGGPLHPPG APPQPTEDSL PSLGDLDFSA

FLSQFPSS
Length:558
Mass (Da):60,072
Last modified:October 1, 1996 - v1
Checksum:i0528D159A4D47B36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13721 mRNA. Translation: BAA02874.1.
PIRiJC2004.
RefSeqiNP_990460.1. NM_205129.1.
UniGeneiGga.604.

Genome annotation databases

GeneIDi396027.
KEGGigga:396027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13721 mRNA. Translation: BAA02874.1.
PIRiJC2004.
RefSeqiNP_990460.1. NM_205129.1.
UniGeneiGga.604.

3D structure databases

ProteinModelPortaliP98152.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396027.
KEGGigga:396027.

Organism-specific databases

CTDi5970.

Phylogenomic databases

HOVERGENiHBG017916.
InParanoidiP98152.
KOiK04735.
PhylomeDBiP98152.

Enzyme and pathway databases

ReactomeiR-GGA-1227892. TRAF6 mediated NF-kB activation.
R-GGA-434001. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-GGA-434131. NFkB activation mediated by RIP1 complexed with activated TLR3.

Miscellaneous databases

PROiP98152.

Family and domain databases

CDDicd01177. IPT_NFkappaB. 1 hit.
Gene3Di2.60.40.10. 1 hit.
2.60.40.340. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR033926. IPT_NFkappaB.
IPR000451. NFkB/Dor.
IPR008967. p53-like_TF_DNA-bd.
IPR030495. RelA.
IPR030492. RHD_CS.
IPR032397. RHD_dimer.
IPR011539. RHD_DNA_bind_dom.
[Graphical view]
PANTHERiPTHR24169. PTHR24169. 2 hits.
PTHR24169:SF1. PTHR24169:SF1. 2 hits.
PfamiPF16179. RHD_dimer. 1 hit.
PF00554. RHD_DNA_bind. 1 hit.
[Graphical view]
PRINTSiPR00057. NFKBTNSCPFCT.
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS01204. REL_1. 1 hit.
PS50254. REL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF65_CHICK
AccessioniPrimary (citable) accession number: P98152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.