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Protein

Transcription factor p65

Gene

RELA

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-GGA-1227892 TRAF6 mediated NF-kB activation
R-GGA-434001 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-GGA-434131 NFkB activation mediated by RIP1 complexed with activated TLR3

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor p65
Alternative name(s):
Nuclear factor NF-kappa-B p65 subunit
Gene namesi
Name:RELA
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002051711 – 558Transcription factor p65Add BLAST558

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei281Phosphoserine; by PKASequence analysis1

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Spleen; lower level in brain.

Interactioni

Subunit structurei

Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-c-Rel complex. Component of the NF-kappa-B p65-p105 complex.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP98152
SMRiP98152
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 311RHDPROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi306 – 309Nuclear localization signalSequence analysis4

Phylogenomic databases

HOVERGENiHBG017916
InParanoidiP98152
KOiK04735
PhylomeDBiP98152

Family and domain databases

CDDicd01177 IPT_NFkappaB, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.340, 1 hit
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR033926 IPT_NFkappaB
IPR000451 NFkB/Dor
IPR008967 p53-like_TF_DNA-bd
IPR030495 RelA
IPR030492 RHD_CS
IPR032397 RHD_dimer
IPR011539 RHD_DNA_bind_dom
IPR037059 RHD_DNA_bind_dom_sf
PANTHERiPTHR24169 PTHR24169, 1 hit
PTHR24169:SF1 PTHR24169:SF1, 1 hit
PfamiView protein in Pfam
PF16179 RHD_dimer, 1 hit
PF00554 RHD_DNA_bind, 1 hit
PRINTSiPR00057 NFKBTNSCPFCT
SMARTiView protein in SMART
SM00429 IPT, 1 hit
SUPFAMiSSF49417 SSF49417, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS01204 REL_1, 1 hit
PS50254 REL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P98152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPADLLPLY LQPEWGEQEP GGATPFVEIL EQPKQRGMRF RYKCEGRSAG
60 70 80 90 100
SIPGEHSTDS ARTHPTIRVN HYRGPGRVRV SLVTKDPPHG PHPHELVGRH
110 120 130 140 150
CQHGYYEAEL SPERCVHSFQ NLGIQCVKKR ELEAAVAERI RTNNNPFNVP
160 170 180 190 200
MEERGAEYDL SAVRLCFQVW VNGPGGLCPL PPVLSQPIYD NRAPSTAELR
210 220 230 240 250
ILPGDRNSGS CQGGDEIFLL CDKVQKEDIE VRFWAEGWEA KGSFAAADVH
260 270 280 290 300
RQVAIVFRTP PFRERSLRHP VTVRMELQRP SDRQRSPPLD FRYLPHQGDL
310 320 330 340 350
QCIEEKRKRT RDTFRAFVQR APLPGLEPNP EPRPPRRIAV PSRPPPAPQQ
360 370 380 390 400
PPSMVGAPPA PLFPLGVPPA SSPTPEPLAE ALLQLQFDDG VGGSGPPPST
410 420 430 440 450
TTTTTTTQCA LGGGIPDPGG SPLDLGALLG DPPFDTIDAA ELQRLLGPPE
460 470 480 490 500
TPPGGIGAGG GFGELLSLPT NFGDPPSSTA ATFGPSPPML LSYPEAITRL
510 520 530 540 550
VQCQTPGGSG GGGPPVGPPQ DLGGPLHPPG APPQPTEDSL PSLGDLDFSA

FLSQFPSS
Length:558
Mass (Da):60,072
Last modified:October 1, 1996 - v1
Checksum:i0528D159A4D47B36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13721 mRNA Translation: BAA02874.1
PIRiJC2004
RefSeqiNP_990460.1, NM_205129.1
UniGeneiGga.604

Genome annotation databases

GeneIDi396027
KEGGigga:396027

Entry informationi

Entry nameiTF65_CHICK
AccessioniPrimary (citable) accession number: P98152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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