Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Complement component C8 alpha chain

Gene

C8A

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Complement alternate pathway, Complement pathway, Cytolysis, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component C8 alpha chain
Alternative name(s):
Complement component 8 subunit alpha
Gene namesi
Name:C8A
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

  • Secreted 1 Publication
  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Secreted as soluble protein. Inserts into the cell membrane of target cells.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Membrane attack complex, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002358921 – 30Sequence analysis10
ChainiPRO_000002359031 – 585Complement component C8 alpha chainAdd BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 74By similarity
Glycosylationi44C-linked (Man)By similarity1
Disulfide bondi50 ↔ 53By similarity
Disulfide bondi84 ↔ 90By similarity
Disulfide bondi96 ↔ 108By similarity
Disulfide bondi102 ↔ 121By similarity
Disulfide bondi115 ↔ 130By similarity
Disulfide bondi140 ↔ 177By similarity
Disulfide bondi194Interchain (with C-60 in C8-gamma chain)PROSITE-ProRule annotation
Glycosylationi438N-linked (GlcNAc...)Sequence analysis1
Glycosylationi543C-linked (Man)By similarity1
Glycosylationi546C-linked (Man)By similarity1
Glycosylationi549C-linked (Man)By similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Heterotrimer of 3 chains: alpha, beta and gamma. The alpha and gamma chains are disulfide bonded. Component of the membrane attack complex (MAC). MAC assembly is initiated by proteolytic cleavage of C5 into C5a and C5b. C5b sequentially binds C6, C7, C8 and multiple copies of the pore-forming subunit C9 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000014764.

Structurei

3D structure databases

ProteinModelPortaliP98136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 91TSP type-1 1PROSITE-ProRule annotationAdd BLAST54
Domaini94 – 132LDL-receptor class APROSITE-ProRule annotationAdd BLAST39
Domaini136 – 499MACPFPROSITE-ProRule annotationAdd BLAST364
Domaini499 – 530EGF-likeAdd BLAST32
Domaini540 – 584TSP type-1 2PROSITE-ProRule annotationAdd BLAST45

Sequence similaritiesi

Belongs to the complement C6/C7/C8/C9 family.Curated
Contains 1 EGF-like domain.Curated
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 1 MACPF domain.PROSITE-ProRule annotation
Contains 2 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG410IE8U. Eukaryota.
ENOG410Y5MF. LUCA.
HOGENOMiHOG000231146.
HOVERGENiHBG005368.
InParanoidiP98136.
KOiK03997.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 2 hits.
[Graphical view]
PRINTSiPR00764. COMPLEMENTC9.
SMARTiSM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMiSSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 2 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P98136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVAAFFTLF LVTCQPAVTA QEKVNQRVNR AATPRAFDCQ LSSWSEWTDC
60 70 80 90 100
FPCQDTKYRH RSLLQPNKFG GTICSGDIWD RASCYSPTAC LRPAQCGQDF
110 120 130 140 150
QCKETGRCLK RHLVCNGEND CLDGSDEDNC EDIRATESDC AQYDPIPGSE
160 170 180 190 200
KAALGYNILT QEEAQSVYDA RYYGGRCETV YNGEWRHVRY DPVCERLHHG
210 220 230 240 250
EDDKYFRKPY NFLKYHFEAR ADTGISFELY VDGNDLFSKV KNDKSHSAGV
260 270 280 290 300
TISAGLTGSP LLGTVGVSGS EDASFLNKLS QYNEKKYNFM RIFTKVQTAH
310 320 330 340 350
FKMRRDDIVL DEGMLQALVE LPEQYNYGMY SKFINDYGTH YITSGSMGGT
360 370 380 390 400
YEYILVLNTE KMESLGVTSE DISSCFGGFG EIQYEKGKIN AQGILSGKHC
410 420 430 440 450
KKSGSGDKEA DKMGQAVKDI ISRVRGGSSG WGGGLSQNGS ATTYRFWGRS
460 470 480 490 500
LKYNPVVIDF EMQPIHEVLL HTNLGHVEAK RQNLRRALDQ YLMEFNACRC
510 520 530 540 550
GPCFNNGKPI LEGTSCRCQC SLGLQGPACE QTEQQGAKAD GHWSCWGSWS
560 570 580
PCTAGTRERR RECNNPAPQN GGAPCPGWRV QTQAC
Length:585
Mass (Da):65,306
Last modified:February 1, 1996 - v1
Checksum:i2FFD3241B0310048
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26981 mRNA. Translation: AAA31191.1.
PIRiI46686.
RefSeqiNP_001075724.1. NM_001082255.1.
UniGeneiOcu.1990.

Genome annotation databases

GeneIDi100009076.
KEGGiocu:100009076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26981 mRNA. Translation: AAA31191.1.
PIRiI46686.
RefSeqiNP_001075724.1. NM_001082255.1.
UniGeneiOcu.1990.

3D structure databases

ProteinModelPortaliP98136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000014764.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009076.
KEGGiocu:100009076.

Organism-specific databases

CTDi731.

Phylogenomic databases

eggNOGiENOG410IE8U. Eukaryota.
ENOG410Y5MF. LUCA.
HOGENOMiHOG000231146.
HOVERGENiHBG005368.
InParanoidiP98136.
KOiK03997.

Family and domain databases

Gene3Di4.10.400.10. 1 hit.
InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001862. MAC_perforin.
IPR020864. MACPF.
IPR020863. MACPF_CS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF01823. MACPF. 1 hit.
PF00090. TSP_1. 2 hits.
[Graphical view]
PRINTSiPR00764. COMPLEMENTC9.
SMARTiSM00192. LDLa. 1 hit.
SM00457. MACPF. 1 hit.
SM00209. TSP1. 2 hits.
[Graphical view]
SUPFAMiSSF57424. SSF57424. 1 hit.
SSF82895. SSF82895. 2 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS00279. MACPF_1. 1 hit.
PS51412. MACPF_2. 1 hit.
PS50092. TSP1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCO8A_RABIT
AccessioniPrimary (citable) accession number: P98136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.