Reviewed, UniProtKB/Swiss-Prot P98121 (URTB_DESRO)

Names and origin

Protein names	Recommended name:     Salivary plasminogen activator beta     EC=3.4.21.68 Alternative name(s):     DSPA beta
Organism	Desmodus rotundus (Vampire bat)
Taxonomic identifier	9430 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Chiroptera › Microchiroptera › Phyllostomidae › Desmodontinae › Desmodus

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably essential to support the feeding habits of this exclusively haematophagous animal. Probable potent thrombolytic agent.
Catalytic activity	Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Secreted
Domain	EGF-like domain Kringle Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Chain	37 – 431	395	Salivary plasminogen activator beta		PRO_0000028342
Regions
	Domain	37 – 75	39	EGF-like
	Domain	82 – 163	82	Kringle
	Domain	180 – 430	251	Peptidase S1
Sites
	Active site	226	1	Charge relay system By similarity
	Active site	275	1	Charge relay system By similarity
	Active site	382	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	139	1	N-linked (GlcNAc...) Potential
	Glycosylation	352	1	N-linked (GlcNAc...) Potential
	Disulfide bond	41 ↔ 52		By similarity
	Disulfide bond	46 ↔ 63		By similarity
	Disulfide bond	65 ↔ 74		By similarity
	Disulfide bond	82 ↔ 163		By similarity
	Disulfide bond	103 ↔ 145		By similarity
	Disulfide bond	134 ↔ 158		By similarity
	Disulfide bond	168 ↔ 299		By similarity
	Disulfide bond	211 ↔ 227		By similarity
	Disulfide bond	219 ↔ 288		By similarity
	Disulfide bond	313 ↔ 388		By similarity
	Disulfide bond	345 ↔ 361		By similarity
	Disulfide bond	378 ↔ 406		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P98121-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 699B5E675B162CBF	FASTA	431	48,222
	10 20 30 40 50 60 MVNTMKTKLL CVLLLCGAVF SLPRQETYRQ LARGSRAYGG CSELRCFNGG TCWQAASFSD 70 80 90 100 110 120 FVCQCPKGYT GKQCEVDTHA TCYKDQGVTY RGTWSTSESG AQCINWNSNL LTRRTYNGRR 130 140 150 160 170 180 SDAITLGLGN HNYCRNPDNN SKPWCYVIKA SKFILEFCSV PVCSKATCGL RKYKEPQLHS 190 200 210 220 230 240 TGGLFTDITS HPWQAAIFAQ NRRSSGERFL CGGILISSCW VLTAAHCFQE RYPPQHLRVV 250 260 270 280 290 300 LGRTYRVKPG KEEQTFEVEK CIIHEEFDDD TYNNDIALLQ LKSGSPQCAQ ESDSVRAICL 310 320 330 340 350 360 PEANLQLPDW TECELSGYGK HKSSSPFYSE QLKEGHVRLY PSSRCTSKFL FNKTVTNNML 370 380 390 400 410 420 CAGDTRSGEI YPNVHDACQG DSGGPLVCMN DNHMTLLGII SWGVGCGEKD IPGVYTKVTN 430 YLGWIRDNMR P

References

[1]	"The plasminogen activator family from the salivary gland of the vampire bat Desmodus rotundus: cloning and expression." Kraetzschmar J., Haendler B., Langer G., Boidol W., Bringmann P., Alagon A., Donner P., Schleuning W.-D. Gene 105:229-237(1991) [PubMed: 1937019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Salivary gland.
[2]	"Plasminogen activators from the saliva of Desmodus rotundus (common vampire bat): unique fibrin specificity." Schleuning W.-D., Alagon A., Boidol W., Bringmann P., Petri T., Kraetzschmar J., Haendler B., Langer G., Baldus B., Witt W., Donner P. Ann. N. Y. Acad. Sci. 667:395-403(1992) [PubMed: 1309059] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63989 mRNA. Translation: AAA31594.1.
PIR	JS0599.
3D structure databases
HSSP	HSSP built from PDB template 1A5I based on UniProtKB P98119.
SMR	P98121. Positions 167-429.
ModBase	Search...
Protein family/group databases
MEROPS	S01.239.
Phylogenomic databases
HOVERGEN	P98121.
Enzyme and pathway databases
BRENDA	3.4.21.68. 291428.
Family and domain databases
InterPro	IPR006209. EGF. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR000001. Kringle. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00008. EGF. 1 hit. PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
ProDom	PD000395. Kringle. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00181. EGF. 1 hit. SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URTB_DESRO

Accession

Primary (citable) accession number: P98121

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)