Reviewed, UniProtKB/Swiss-Prot P98084 (APBA2_MOUSE)

Names and origin

Protein names

Recommended name:
    Amyloid beta A4 precursor protein-binding family A member 2
Alternative name(s):
    Neuron-specific X11L protein
    Neuronal Munc18-1-interacting protein 2
      Short name=Mint-2
    Adapter protein X11beta

Gene names

Name:	Apba2
Synonyms:	X11l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative function in synaptic vesicle exocytosis by binding to STXBP1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.
Subunit structure	Part of a multimeric complex containing STXBP1 and syntaxin-1. Binds to the cytoplasmic domain of amyloid protein beta, and to the nuclear factor NF-kappa-B/p65 via its PDZ domain. Interacts with the amino-terminal domain of APBA2BP By similarity.
Tissue specificity	Specifically expressed in neurons, predominantly of the cerebellum, hippocampus, and spinal cord. Lesser extent in neurons of the cerebral cortex and anterior thalmic nuclei.
Domain	Composed of an N-terminal domain that binds STXBP1, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
Sequence similarities	Contains 2 PDZ (DHR) domains. Contains 1 PID domain.
Caution	Was originally (Ref.1) thought to be the ortholog of human X11 (APBA1).

Ontologies

Keywords
Biological process	Protein transport Transport
Domain	Repeat
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	in utero embryonic development Inferred from genetic interaction. Source: MGI locomotory behavior Inferred from genetic interaction. Source: MGI multicellular organism growth Inferred from genetic interaction. Source: MGI protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of gene expression Inferred from genetic interaction. Source: MGI synaptic transmission Inferred from genetic interaction. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
App	P12023	1	EBI-81669,EBI-78814

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 750	750	Amyloid beta A4 precursor protein-binding family A member 2		PRO_0000064617
Regions
	Domain	367 – 556	190	PID
	Domain	569 – 654	86	PDZ 1
	Domain	660 – 736	77	PDZ 2
	Region	185 – 271	87	STXBP1-binding
Amino acid modifications
	Modified residue	209	1	Phosphoserine
Experimental info
	Sequence conflict	313	1	K → M in AAA73936. Ref.2
	Sequence conflict	388	1	N → T in AAA73936. Ref.2
	Sequence conflict	402	1	V → A in AAA73936. Ref.2
	Sequence conflict	677 – 679	3	QNG → RW in AAA73936. Ref.2
	Sequence conflict	688 – 690	3	GIA → VLQ in AAA73936. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P98084-1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: FC231C966DB467DE	FASTA	750	82,758
	10 20 30 40 50 60 MAHRKRQSTA SSMLDHRARP GPIPHDQEPE SEDTELPLES YVPTGLELGT LRPESPTPEE 70 80 90 100 110 120 QECHNHSPDG DSSSDYVNNT SEEEDYDEGL PEEEEGVTYY IRYCPEDDSY LEGMDCNGEE 130 140 150 160 170 180 YIAHGAHPVD TDECQEAVED WTDSVGPHTH SHGAENSQEY PDGHLPIPED DPTVLEVHDQ 190 200 210 220 230 240 EEDGHYCSSK ESYQDYYPPE TNGNTGGASP YRMRRGDGDL EEQEEDIDQI VAEIKMSLSM 250 260 270 280 290 300 TSITSASEAS PEHMPELDPG DSTEACPPSD TGHGPGRQEA RPKSLNLPPE VKHPGDLQRG 310 320 330 340 350 360 LKTKTRTPEE RPKWPQEQVC NGLEQPRKQQ RSDLNGPTDN NNIPETKKVA SFPSFVAVPG 370 380 390 400 410 420 PCEAEDLIDG IIFAANYLGS TQLLSERNPS KNIRMMQAQE AVSRVKRMQK AAKIKKKANS 430 440 450 460 470 480 EGDAQTLTEV DLFISTQRIK VLNADTQETM MDHALRTISY IADIGNIVVL MARRRMPRSA 490 500 510 520 530 540 SQDCIETTPG AQEGKKQYKM ICHVFESEDA QLIAQSIGQA FSVAYQEFLR ANGINPEDLS 550 560 570 580 590 600 QKEYSDIINT QEMYNDDLIH FSNSENCKEL QLEKHKGEIL GVVVVESGWG SILPTVILAN 610 620 630 640 650 660 MMNGGPAARS GKLSIGDQIM SINGTSLVGL PLATCQGIIK GLKNQTQVKL NIVSCPPVTT 670 680 690 700 710 720 VLIKRPDLKY QLGFSVQNGI ICSLMRGGIA ERGGVRVGHR IIEINGQSVV ATAHEKIVQA 730 740 750 LSNSVGEIHM KTMPAAMFRL LTGQETPLYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Comparison of primary structure of a neuron-specific protein, X11, between human and mouse." Duclos F., Koenig M. Mamm. Genome 6:57-58(1995) [PubMed: 7719031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-750. Tissue: Brain.
[3]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC060269 mRNA. Translation: AAH60269.1. BC057620 mRNA. Translation: AAH57620.1. L34676 mRNA. Translation: AAA73936.1.
RefSeq	NP_031487.1.
UniGene	Mm.4657
3D structure databases
HSSP	HSSP built from PDB template 1AQC based on UniProtKB Q02410.
SMR	P98084. Positions 568-750.
ModBase	Search...
Protein-protein interaction databases
IntAct	P98084.
PTM databases
PhosphoSite	P98084.
Genome annotation databases
Ensembl	ENSMUSG00000030519. Mus musculus. [Contig view]
GeneID	11784.
KEGG	mmu:11784.
Organism-specific databases
MGI	MGI:1261791. Apba2.
Phylogenomic databases
HOGENOM	P98084.
HOVERGEN	P98084.
Gene expression databases
ArrayExpress	P98084.
GermOnline	ENSMUSG00000030519. Mus musculus.
Family and domain databases
InterPro	IPR001478. PDZ. IPR011993. PH_type. IPR006020. PTB_PID. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00595. PDZ. 2 hits. PF00640. PID. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 2 hits. SM00462. PTB. 1 hit. [Graphical view]
PROSITE	PS50106. PDZ. 2 hits. PS01179. PID. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279597.
SOURCE	Search...

Entry information

Entry name

APBA2_MOUSE

Accession

Primary (citable) accession number: P98084
Secondary accession number(s): Q6PAJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	July 19, 2005
Last modified:	November 25, 2008
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)