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Protein

Amyloid beta A4 precursor protein-binding family A member 2

Gene

Apba2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative function in synaptic vesicle exocytosis by binding to STXBP1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • locomotory behavior Source: MGI
  • multicellular organism growth Source: MGI
  • protein transport Source: UniProtKB-KW
  • regulation of gene expression Source: MGI
  • synaptic transmission Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid beta A4 precursor protein-binding family A member 2
Alternative name(s):
Adapter protein X11beta
Neuron-specific X11L protein
Neuronal Munc18-1-interacting protein 2
Short name:
Mint-2
Gene namesi
Name:Apba2
Synonyms:X11l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1261791. Apba2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Amyloid beta A4 precursor protein-binding family A member 2PRO_0000064617Add
BLAST

Proteomic databases

MaxQBiP98084.
PaxDbiP98084.
PRIDEiP98084.

PTM databases

PhosphoSiteiP98084.

Expressioni

Tissue specificityi

Specifically expressed in neurons, predominantly of the cerebellum, hippocampus, and spinal cord. Lesser extent in neurons of the cerebral cortex and anterior thalmic nuclei.

Gene expression databases

BgeeiP98084.
ExpressionAtlasiP98084. baseline and differential.
GenevisibleiP98084. MM.

Interactioni

Subunit structurei

Part of a multimeric complex containing STXBP1 and syntaxin-1. Binds to the cytoplasmic domain of amyloid protein beta, and to the nuclear factor NF-kappa-B/p65 via its PDZ domain. Interacts with the N-terminal domain of APBA2BP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AppP120232EBI-81669,EBI-78814
PSEN1P497682EBI-81669,EBI-297277From a different organism.

Protein-protein interaction databases

IntActiP98084. 5 interactions.
STRINGi10090.ENSMUSP00000032732.

Structurei

3D structure databases

ProteinModelPortaliP98084.
SMRiP98084. Positions 364-750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini367 – 556190PIDPROSITE-ProRule annotationAdd
BLAST
Domaini569 – 65486PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini660 – 73677PDZ 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni185 – 27187STXBP1-bindingAdd
BLAST

Domaini

Composed of an N-terminal domain that binds STXBP1, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG283022.
GeneTreeiENSGT00390000013578.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiP98084.
OMAiRHEGRPK.
OrthoDBiEOG78PV8H.
PhylomeDBiP98084.
TreeFamiTF315245.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030529. Apba2.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF12. PTHR12345:SF12. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P98084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHRKRQSTA SSMLDHRARP GPIPHDQEPE SEDTELPLES YVPTGLELGT
60 70 80 90 100
LRPESPTPEE QECHNHSPDG DSSSDYVNNT SEEEDYDEGL PEEEEGVTYY
110 120 130 140 150
IRYCPEDDSY LEGMDCNGEE YIAHGAHPVD TDECQEAVED WTDSVGPHTH
160 170 180 190 200
SHGAENSQEY PDGHLPIPED DPTVLEVHDQ EEDGHYCSSK ESYQDYYPPE
210 220 230 240 250
TNGNTGGASP YRMRRGDGDL EEQEEDIDQI VAEIKMSLSM TSITSASEAS
260 270 280 290 300
PEHMPELDPG DSTEACPPSD TGHGPGRQEA RPKSLNLPPE VKHPGDLQRG
310 320 330 340 350
LKTKTRTPEE RPKWPQEQVC NGLEQPRKQQ RSDLNGPTDN NNIPETKKVA
360 370 380 390 400
SFPSFVAVPG PCEAEDLIDG IIFAANYLGS TQLLSERNPS KNIRMMQAQE
410 420 430 440 450
AVSRVKRMQK AAKIKKKANS EGDAQTLTEV DLFISTQRIK VLNADTQETM
460 470 480 490 500
MDHALRTISY IADIGNIVVL MARRRMPRSA SQDCIETTPG AQEGKKQYKM
510 520 530 540 550
ICHVFESEDA QLIAQSIGQA FSVAYQEFLR ANGINPEDLS QKEYSDIINT
560 570 580 590 600
QEMYNDDLIH FSNSENCKEL QLEKHKGEIL GVVVVESGWG SILPTVILAN
610 620 630 640 650
MMNGGPAARS GKLSIGDQIM SINGTSLVGL PLATCQGIIK GLKNQTQVKL
660 670 680 690 700
NIVSCPPVTT VLIKRPDLKY QLGFSVQNGI ICSLMRGGIA ERGGVRVGHR
710 720 730 740 750
IIEINGQSVV ATAHEKIVQA LSNSVGEIHM KTMPAAMFRL LTGQETPLYI
Length:750
Mass (Da):82,758
Last modified:July 19, 2005 - v2
Checksum:iFC231C966DB467DE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti313 – 3131K → M in AAA73936 (PubMed:7719031).Curated
Sequence conflicti388 – 3881N → T in AAA73936 (PubMed:7719031).Curated
Sequence conflicti402 – 4021V → A in AAA73936 (PubMed:7719031).Curated
Sequence conflicti677 – 6793QNG → RW in AAA73936 (PubMed:7719031).Curated
Sequence conflicti688 – 6903GIA → VLQ in AAA73936 (PubMed:7719031).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC060269 mRNA. Translation: AAH60269.1.
BC057620 mRNA. Translation: AAH57620.1.
L34676 mRNA. Translation: AAA73936.1.
CCDSiCCDS21335.1.
RefSeqiNP_001278095.1. NM_001291166.1.
NP_001278096.1. NM_001291167.1.
NP_031487.1. NM_007461.2.
XP_006540636.1. XM_006540573.2.
XP_006540637.1. XM_006540574.2.
UniGeneiMm.4657.

Genome annotation databases

EnsembliENSMUST00000032732; ENSMUSP00000032732; ENSMUSG00000030519.
GeneIDi11784.
KEGGimmu:11784.
UCSCiuc009hgj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC060269 mRNA. Translation: AAH60269.1.
BC057620 mRNA. Translation: AAH57620.1.
L34676 mRNA. Translation: AAA73936.1.
CCDSiCCDS21335.1.
RefSeqiNP_001278095.1. NM_001291166.1.
NP_001278096.1. NM_001291167.1.
NP_031487.1. NM_007461.2.
XP_006540636.1. XM_006540573.2.
XP_006540637.1. XM_006540574.2.
UniGeneiMm.4657.

3D structure databases

ProteinModelPortaliP98084.
SMRiP98084. Positions 364-750.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP98084. 5 interactions.
STRINGi10090.ENSMUSP00000032732.

PTM databases

PhosphoSiteiP98084.

Proteomic databases

MaxQBiP98084.
PaxDbiP98084.
PRIDEiP98084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032732; ENSMUSP00000032732; ENSMUSG00000030519.
GeneIDi11784.
KEGGimmu:11784.
UCSCiuc009hgj.2. mouse.

Organism-specific databases

CTDi321.
MGIiMGI:1261791. Apba2.

Phylogenomic databases

eggNOGiNOG283022.
GeneTreeiENSGT00390000013578.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiP98084.
OMAiRHEGRPK.
OrthoDBiEOG78PV8H.
PhylomeDBiP98084.
TreeFamiTF315245.

Miscellaneous databases

ChiTaRSiApba2. mouse.
NextBioi279597.
PROiP98084.
SOURCEiSearch...

Gene expression databases

BgeeiP98084.
ExpressionAtlasiP98084. baseline and differential.
GenevisibleiP98084. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030529. Apba2.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF12. PTHR12345:SF12. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Comparison of primary structure of a neuron-specific protein, X11, between human and mouse."
    Duclos F., Koenig M.
    Mamm. Genome 6:57-58(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-750.
    Tissue: Brain.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.

Entry informationi

Entry nameiAPBA2_MOUSE
AccessioniPrimary (citable) accession number: P98084
Secondary accession number(s): Q6PAJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 19, 2005
Last modified: June 24, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be the ortholog of human X11 (APBA1).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.