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P98074

- ENTK_PIG

UniProt

P98074 - ENTK_PIG

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Protein

Enteropeptidase

Gene
TMPRSS15, ENTK, PRSS7
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

Catalytic activityi

Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei840 – 8401Charge relay system By similarity
Active sitei891 – 8911Charge relay system By similarity
Active sitei986 – 9861Charge relay system By similarity

GO - Molecular functioni

  1. scavenger receptor activity Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.156.

Names & Taxonomyi

Protein namesi
Recommended name:
Enteropeptidase (EC:3.4.21.9)
Alternative name(s):
Enterokinase
Serine protease 7
Transmembrane protease serine 15
Cleaved into the following 3 chains:
Gene namesi
Name:TMPRSS15
Synonyms:ENTK, PRSS7
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1817Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei19 – 4729Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini48 – 1034987Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 5151PRO_0000027723Add
BLAST
Chaini52 – 11766Enteropeptidase non-catalytic mini chainPRO_0000027724Add
BLAST
Chaini118 – 799682Enteropeptidase non-catalytic heavy chainPRO_0000027725Add
BLAST
Chaini800 – 1034235Enteropeptidase catalytic light chainPRO_0000027726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi147 – 1471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi170 – 1701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi194 – 1941N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi199 ↔ 212 By similarity
Disulfide bondi206 ↔ 225 By similarity
Disulfide bondi219 ↔ 236 By similarity
Disulfide bondi240 ↔ 268 By similarity
Glycosylationi283 – 2831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi343 – 3431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi350 – 3501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi403 – 4031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi455 – 4551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi485 – 4851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi518 – 5181N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi539 ↔ 567 By similarity
Glycosylationi549 – 5491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi645 – 6451N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi658 ↔ 670 By similarity
Disulfide bondi665 ↔ 683 By similarity
Disulfide bondi677 ↔ 692 By similarity
Glycosylationi697 – 6971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi701 – 7011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi721 – 7211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi740 – 7401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi761 – 7611N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi772 ↔ 782 By similarity
Disulfide bondi787 ↔ 911Interchain (between heavy and light chains) By similarity
Glycosylationi804 – 8041N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi825 ↔ 841 By similarity
Glycosylationi863 – 8631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi902 – 9021N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi925 ↔ 992 By similarity
Disulfide bondi956 ↔ 971 By similarity
Glycosylationi964 – 9641N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi982 ↔ 1010 By similarity

Post-translational modificationi

The chains are derived from a single precursor that is cleaved by a trypsin-like protease.
The mini chain may be cleaved by elastase.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Interactioni

Subunit structurei

Heterotrimer of a catalytic (light) chain, a multidomain (heavy) chain, and a mini chain.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012801.

Structurei

3D structure databases

ProteinModelPortaliP98074.
SMRiP98074. Positions 800-1034.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 169116SEAAdd
BLAST
Domaini197 – 23842LDL-receptor class A 1Add
BLAST
Domaini240 – 349110CUB 1Add
BLAST
Domaini357 – 519163MAMAdd
BLAST
Domaini539 – 649111CUB 2Add
BLAST
Domaini656 – 69439LDL-receptor class A 2Add
BLAST
Domaini693 – 78694SRCRAdd
BLAST
Domaini800 – 1034235Peptidase S1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 MAM domain.
Contains 1 SEA domain.
Contains 1 SRCR domain.

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000112380.
HOVERGENiHBG005588.
KOiK01316.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
3.10.250.10. 1 hit.
3.30.70.960. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR000998. MAM_dom.
IPR011163. Pept_S1A_enterop.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF00629. MAM. 1 hit.
PF01390. SEA. 1 hit.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001138. Enteropeptidase. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 2 hits.
SM00137. MAM. 1 hit.
SM00200. SEA. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 2 hits.
PS50068. LDLRA_2. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50024. SEA. 1 hit.
PS00420. SRCR_1. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P98074-1 [UniParc]FASTAAdd to Basket

« Hide

MGSKRIIPSR HRSLSTYEVM FTALFAILMV LCAGLIAVSW LTIKGSEKDA     50
ALGKSHEARG TMKITSGVTY NPNLQDKLSV DFKVLAFDIQ QMIGEIFQSS 100
NLKNEYKNSR VLQFENGSVI VIFDLLFAQW VSDENIKEEL IQGIEANKSS 150
QLVAFHIDVN SIDITESLEN YSTTSPSTTS DKLTTSSPPA TPGNVSIECL 200
PGSRPCADAL KCIAVDLFCD GELNCPDGSD EDSKICATAC DGKFLLTESS 250
GSFDAAQYPK LSEASVVCQW IIRVNQGLSI ELNFSYFNTY SMDVLNIYEG 300
VGSSKILRAS LWLMNPGTIR IFSNQVTVTF LIESDENDYI GFNATYTAFN 350
STELNNDEKI NCNFEDGFCF WIQDLNDDNE WERIQGTTFP PFTGPNFDHT 400
FGNASGFYIS TPTGPGGRQE RVGLLSLPLE PTLEPVCLSF WYYMYGENVY 450
KLSINISNDQ NIEKIIFQKE GNYGENWNYG QVTLNETVEF KVAFNAFKNQ 500
FLSDIALDDI SLTYGICNVS LYPEPTLVPT SPPELPTDCG GPFELWEPNT 550
TFTSMNFPNN YPNQAFCVWN LNAQKGKNIQ LHFEEFDLEN IADVVEIRDG 600
EEDDSLLLAV YTGPGPVEDV FSTTNRMTVL FITNDALTKG GFKANFTTGY 650
HLGIPEPCKE DNFQCENGEC VLLVNLCDGF SHCKDGSDEA HCVRFLNGTA 700
NNSGLVQFRI QSIWHTACAE NWTTQTSDDV CQLLGLGTGN SSMPFFSSGG 750
GPFVKLNTAP NGSLILTASE QCFEDSLILL QCNHKSCGKK QVAQEVSPKI 800
VGGNDSREGA WPWVVALYYN GQLLCGASLV SRDWLVSAAH CVYGRNLEPS 850
KWKAILGLHM TSNLTSPQIV TRLIDEIVIN PHYNRRRKDS DIAMMHLEFK 900
VNYTDYIQPI CLPEENQVFP PGRICSIAGW GKVIYQGSPA DILQEADVPL 950
LSNEKCQQQM PEYNITENMM CAGYEEGGID SCQGDSGGPL MCLENNRWLL 1000
AGVTSFGYQC ALPNRPGVYA RVPKFTEWIQ SFLH 1034
Length:1,034
Mass (Da):114,776
Last modified:February 1, 1996 - v1
Checksum:i0388C64CF64CC368
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30799 mRNA. Translation: BAA06459.1.
PIRiA53663.
RefSeqiNP_001001259.1. NM_001001259.1.
UniGeneiSsc.298.

Genome annotation databases

GeneIDi397152.
KEGGissc:397152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30799 mRNA. Translation: BAA06459.1 .
PIRi A53663.
RefSeqi NP_001001259.1. NM_001001259.1.
UniGenei Ssc.298.

3D structure databases

ProteinModelPortali P98074.
SMRi P98074. Positions 800-1034.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000012801.

Protein family/group databases

MEROPSi S01.156.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397152.
KEGGi ssc:397152.

Organism-specific databases

CTDi 5651.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000112380.
HOVERGENi HBG005588.
KOi K01316.

Family and domain databases

Gene3Di 2.60.120.290. 2 hits.
3.10.250.10. 1 hit.
3.30.70.960. 1 hit.
4.10.400.10. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR000998. MAM_dom.
IPR011163. Pept_S1A_enterop.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF00629. MAM. 1 hit.
PF01390. SEA. 1 hit.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001138. Enteropeptidase. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00042. CUB. 2 hits.
SM00192. LDLa. 2 hits.
SM00137. MAM. 1 hit.
SM00200. SEA. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEi PS01180. CUB. 2 hits.
PS01209. LDLRA_1. 2 hits.
PS50068. LDLRA_2. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50024. SEA. 1 hit.
PS00420. SRCR_1. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structural characterization of porcine enteropeptidase."
    Matsushima M., Ichinose M., Yahagi N., Kakei N., Tsukada S., Miki K., Kurokawa K., Tashiro K., Shiokawa K., Shinomiya K., Umeyama H., Inoue H., Takahashi T., Takahashi K.
    J. Biol. Chem. 269:19976-19982(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Duodenal mucosa.

Entry informationi

Entry nameiENTK_PIG
AccessioniPrimary (citable) accession number: P98074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi