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Protein

Enteropeptidase

Gene

TMPRSS15

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

Catalytic activityi

Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei841Charge relay system1 Publication1
Active sitei892Charge relay system1 Publication1
Active sitei987Charge relay system1 Publication1

GO - Molecular functioni

GO - Biological processi

  • protein digestion Source: AgBase
  • trypsinogen activation Source: AgBase

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.9 908

Protein family/group databases

MEROPSiS01.156

Names & Taxonomyi

Protein namesi
Recommended name:
Enteropeptidase (EC:3.4.21.9)
Alternative name(s):
Enterokinase
Serine protease 7
Transmembrane protease serine 15
Cleaved into the following 2 chains:
Gene namesi
Name:TMPRSS15
Synonyms:ENTK, PRSS7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Organism-specific databases

VGNCiVGNC:36139 TMPRSS15

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 18CytoplasmicSequence analysisAdd BLAST17
Transmembranei19 – 47Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST29
Topological domaini48 – 1035ExtracellularSequence analysisAdd BLAST988

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi889K → A: Prevents the cleavage of trypsinogen. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000277172 – 800Enteropeptidase non-catalytic heavy chainAdd BLAST799
ChainiPRO_0000027718801 – 1035Enteropeptidase catalytic light chainAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi147N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi194N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi199 ↔ 212By similarity
Disulfide bondi206 ↔ 225By similarity
Disulfide bondi219 ↔ 236By similarity
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi240 ↔ 269By similarity
Glycosylationi263N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi264N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi404N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi456N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi486N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi519N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi540 ↔ 568By similarity
Glycosylationi550N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi646N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi659 ↔ 671By similarity
Disulfide bondi666 ↔ 684By similarity
Disulfide bondi678 ↔ 693By similarity
Glycosylationi698N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi722N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi741N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi762N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi773 ↔ 783By similarity
Disulfide bondi788 ↔ 912Interchain (between heavy and light chains)
Disulfide bondi826 ↔ 842By similarity
Glycosylationi864N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi903N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi926 ↔ 993By similarity
Disulfide bondi957 ↔ 972By similarity
Glycosylationi965N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi983 ↔ 1011By similarity

Post-translational modificationi

The chains are derived from a single precursor that is cleaved by a trypsin-like protease.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Myristate, Zymogen

Proteomic databases

PaxDbiP98072
PRIDEiP98072

Expressioni

Tissue specificityi

Intestinal brush border.

Gene expression databases

BgeeiENSBTAG00000000597

Interactioni

Subunit structurei

Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000788

Structurei

Secondary structure

11035
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi815 – 820Combined sources6
Beta strandi823 – 830Combined sources8
Beta strandi832 – 838Combined sources7
Helixi840 – 843Combined sources4
Helixi850 – 852Combined sources3
Beta strandi853 – 858Combined sources6
Beta strandi871 – 880Combined sources10
Turni886 – 889Combined sources4
Beta strandi894 – 900Combined sources7
Beta strandi925 – 935Combined sources11
Beta strandi945 – 951Combined sources7
Helixi954 – 960Combined sources7
Beta strandi970 – 973Combined sources4
Beta strandi990 – 995Combined sources6
Beta strandi998 – 1007Combined sources10
Beta strandi1009 – 1012Combined sources4
Beta strandi1018 – 1022Combined sources5
Helixi1023 – 1025Combined sources3
Helixi1027 – 1031Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EKBX-ray2.30A788-800[»]
B801-1035[»]
ProteinModelPortaliP98072
SMRiP98072
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP98072

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 169SEAPROSITE-ProRule annotationAdd BLAST116
Domaini197 – 238LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST42
Domaini240 – 350CUB 1PROSITE-ProRule annotationAdd BLAST111
Domaini358 – 520MAMPROSITE-ProRule annotationAdd BLAST163
Domaini540 – 650CUB 2PROSITE-ProRule annotationAdd BLAST111
Domaini657 – 695LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST39
Domaini694 – 787SRCRPROSITE-ProRule annotationAdd BLAST94
Domaini801 – 1035Peptidase S1PROSITE-ProRule annotationAdd BLAST235

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118962
HOGENOMiHOG000112380
HOVERGENiHBG005588
InParanoidiP98072
KOiK01316
OMAiNWNYGQV
OrthoDBiEOG091G0DF7
TreeFamiTF351678

Family and domain databases

CDDicd00041 CUB, 2 hits
cd00112 LDLa, 2 hits
cd06263 MAM, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.60.120.290, 2 hits
3.10.250.10, 1 hit
3.30.70.960, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000859 CUB_dom
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR000998 MAM_dom
IPR011163 Pept_S1A_enterop
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR000082 SEA_dom
IPR036364 SEA_dom_sf
IPR035914 Sperma_CUB_dom_sf
IPR001190 SRCR
IPR017448 SRCR-like_dom
IPR036772 SRCR-like_dom_sf
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF00057 Ldl_recept_a, 1 hit
PF00629 MAM, 1 hit
PF01390 SEA, 1 hit
PF00530 SRCR, 1 hit
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001138 Enteropeptidase, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00042 CUB, 2 hits
SM00192 LDLa, 2 hits
SM00137 MAM, 1 hit
SM00200 SEA, 1 hit
SM00202 SR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF49899 SSF49899, 1 hit
SSF50494 SSF50494, 1 hit
SSF56487 SSF56487, 1 hit
SSF57424 SSF57424, 2 hits
SSF82671 SSF82671, 1 hit
PROSITEiView protein in PROSITE
PS01180 CUB, 2 hits
PS01209 LDLRA_1, 2 hits
PS50068 LDLRA_2, 2 hits
PS00740 MAM_1, 1 hit
PS50060 MAM_2, 1 hit
PS50024 SEA, 1 hit
PS00420 SRCR_1, 1 hit
PS50287 SRCR_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P98072-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSKRSVPSR HRSLTTYEVM FAVLFVILVA LCAGLIAVSW LSIQGSVKDA
60 70 80 90 100
AFGKSHEARG TLKIISGATY NPHLQDKLSV DFKVLAFDIQ QMIDDIFQSS
110 120 130 140 150
NLKNEYKNSR VLQFENGSII VIFDLLFDQW VSDKNVKEEL IQGIEANKSS
160 170 180 190 200
QLVTFHIDLN SIDITASLEN FSTISPATTS EKLTTSIPLA TPGNVSIECP
210 220 230 240 250
PDSRLCADAL KCIAIDLFCD GELNCPDGSD EDNKTCATAC DGRFLLTGSS
260 270 280 290 300
GSFEALHYPK PSNNTSAVCR WIIRVNQGLS IQLNFDYFNT YYADVLNIYE
310 320 330 340 350
GMGSSKILRA SLWSNNPGII RIFSNQVTAT FLIQSDESDY IGFKVTYTAF
360 370 380 390 400
NSKELNNYEK INCNFEDGFC FWIQDLNDDN EWERTQGSTF PPSTGPTFDH
410 420 430 440 450
TFGNESGFYI STPTGPGGRR ERVGLLTLPL DPTPEQACLS FWYYMYGENV
460 470 480 490 500
YKLSINISSD QNMEKTIFQK EGNYGQNWNY GQVTLNETVE FKVSFYGFKN
510 520 530 540 550
QILSDIALDD ISLTYGICNV SVYPEPTLVP TPPPELPTDC GGPHDLWEPN
560 570 580 590 600
TTFTSINFPN SYPNQAFCIW NLNAQKGKNI QLHFQEFDLE NIADVVEIRD
610 620 630 640 650
GEGDDSLFLA VYTGPGPVND VFSTTNRMTV LFITDNMLAK QGFKANFTTG
660 670 680 690 700
YGLGIPEPCK EDNFQCKDGE CIPLVNLCDG FPHCKDGSDE AHCVRLFNGT
710 720 730 740 750
TDSSGLVQFR IQSIWHVACA ENWTTQISDD VCQLLGLGTG NSSVPTFSTG
760 770 780 790 800
GGPYVNLNTA PNGSLILTPS QQCLEDSLIL LQCNYKSCGK KLVTQEVSPK
810 820 830 840 850
IVGGSDSREG AWPWVVALYF DDQQVCGASL VSRDWLVSAA HCVYGRNMEP
860 870 880 890 900
SKWKAVLGLH MASNLTSPQI ETRLIDQIVI NPHYNKRRKN NDIAMMHLEM
910 920 930 940 950
KVNYTDYIQP ICLPEENQVF PPGRICSIAG WGALIYQGST ADVLQEADVP
960 970 980 990 1000
LLSNEKCQQQ MPEYNITENM VCAGYEAGGV DSCQGDSGGP LMCQENNRWL
1010 1020 1030
LAGVTSFGYQ CALPNRPGVY ARVPRFTEWI QSFLH
Length:1,035
Mass (Da):114,887
Last modified:February 1, 1996 - v1
Checksum:iE207970B08296E13
GO
Isoform Short (identifier: P98072-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     166-192: Missing.

Show »
Length:1,008
Mass (Da):112,127
Checksum:iE987B6FAE94E9FF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti808R → Y AA sequence (PubMed:1799406).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005386166 – 192Missing in isoform Short. CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09859 mRNA Translation: AAB40026.1
L19663 mRNA Translation: AAA16035.1
PIRiA43090
RefSeqiNP_776864.1, NM_174439.2 [P98072-1]
UniGeneiBt.66152

Genome annotation databases

EnsembliENSBTAT00000000788; ENSBTAP00000000788; ENSBTAG00000000597 [P98072-1]
ENSBTAT00000064206; ENSBTAP00000054149; ENSBTAG00000000597 [P98072-2]
GeneIDi282009
KEGGibta:282009

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiENTK_BOVIN
AccessioniPrimary (citable) accession number: P98072
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health