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Protein

Enteropeptidase

Gene

TMPRSS15

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

Catalytic activityi

Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei841Charge relay system1 Publication1
Active sitei892Charge relay system1 Publication1
Active sitei987Charge relay system1 Publication1

GO - Molecular functioni

GO - Biological processi

  • protein digestion Source: AgBase
  • trypsinogen activation Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.9. 908.

Protein family/group databases

MEROPSiS01.156.

Names & Taxonomyi

Protein namesi
Recommended name:
Enteropeptidase (EC:3.4.21.9)
Alternative name(s):
Enterokinase
Serine protease 7
Transmembrane protease serine 15
Cleaved into the following 2 chains:
Gene namesi
Name:TMPRSS15
Synonyms:ENTK, PRSS7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 18CytoplasmicSequence analysisAdd BLAST17
Transmembranei19 – 47Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST29
Topological domaini48 – 1035ExtracellularSequence analysisAdd BLAST988

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi889K → A: Prevents the cleavage of trypsinogen. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000277172 – 800Enteropeptidase non-catalytic heavy chainAdd BLAST799
ChainiPRO_0000027718801 – 1035Enteropeptidase catalytic light chainAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Glycosylationi116N-linked (GlcNAc...)Sequence analysis1
Glycosylationi147N-linked (GlcNAc...)Sequence analysis1
Glycosylationi170N-linked (GlcNAc...)Sequence analysis1
Glycosylationi194N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi199 ↔ 212By similarity
Disulfide bondi206 ↔ 225By similarity
Disulfide bondi219 ↔ 236By similarity
Glycosylationi233N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi240 ↔ 269By similarity
Glycosylationi263N-linked (GlcNAc...)Sequence analysis1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1
Glycosylationi404N-linked (GlcNAc...)Sequence analysis1
Glycosylationi456N-linked (GlcNAc...)Sequence analysis1
Glycosylationi486N-linked (GlcNAc...)Sequence analysis1
Glycosylationi519N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi540 ↔ 568By similarity
Glycosylationi550N-linked (GlcNAc...)Sequence analysis1
Glycosylationi646N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi659 ↔ 671By similarity
Disulfide bondi666 ↔ 684By similarity
Disulfide bondi678 ↔ 693By similarity
Glycosylationi698N-linked (GlcNAc...)Sequence analysis1
Glycosylationi722N-linked (GlcNAc...)Sequence analysis1
Glycosylationi741N-linked (GlcNAc...)Sequence analysis1
Glycosylationi762N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi773 ↔ 783By similarity
Disulfide bondi788 ↔ 912Interchain (between heavy and light chains)
Disulfide bondi826 ↔ 842By similarity
Glycosylationi864N-linked (GlcNAc...)Sequence analysis1
Glycosylationi903N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi926 ↔ 993By similarity
Disulfide bondi957 ↔ 972By similarity
Glycosylationi965N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi983 ↔ 1011By similarity

Post-translational modificationi

The chains are derived from a single precursor that is cleaved by a trypsin-like protease.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Myristate, Zymogen

Proteomic databases

PaxDbiP98072.
PRIDEiP98072.

Expressioni

Tissue specificityi

Intestinal brush border.

Gene expression databases

BgeeiENSBTAG00000000597.

Interactioni

Subunit structurei

Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000788.

Structurei

Secondary structure

11035
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi815 – 820Combined sources6
Beta strandi823 – 830Combined sources8
Beta strandi832 – 838Combined sources7
Helixi840 – 843Combined sources4
Helixi850 – 852Combined sources3
Beta strandi853 – 858Combined sources6
Beta strandi871 – 880Combined sources10
Turni886 – 889Combined sources4
Beta strandi894 – 900Combined sources7
Beta strandi925 – 935Combined sources11
Beta strandi945 – 951Combined sources7
Helixi954 – 960Combined sources7
Beta strandi970 – 973Combined sources4
Beta strandi990 – 995Combined sources6
Beta strandi998 – 1007Combined sources10
Beta strandi1009 – 1012Combined sources4
Beta strandi1018 – 1022Combined sources5
Helixi1023 – 1025Combined sources3
Helixi1027 – 1031Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EKBX-ray2.30A788-800[»]
B801-1035[»]
ProteinModelPortaliP98072.
SMRiP98072.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP98072.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 169SEAPROSITE-ProRule annotationAdd BLAST116
Domaini197 – 238LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST42
Domaini240 – 350CUB 1PROSITE-ProRule annotationAdd BLAST111
Domaini358 – 520MAMPROSITE-ProRule annotationAdd BLAST163
Domaini540 – 650CUB 2PROSITE-ProRule annotationAdd BLAST111
Domaini657 – 695LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST39
Domaini694 – 787SRCRPROSITE-ProRule annotationAdd BLAST94
Domaini801 – 1035Peptidase S1PROSITE-ProRule annotationAdd BLAST235

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SEA domain.PROSITE-ProRule annotation
Contains 1 SRCR domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000112380.
HOVERGENiHBG005588.
InParanoidiP98072.
KOiK01316.
OMAiFEDGFCF.
OrthoDBiEOG091G0DF7.
TreeFamiTF351678.

Family and domain databases

CDDicd00041. CUB. 2 hits.
cd06263. MAM. 1 hit.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
3.10.250.10. 1 hit.
3.30.70.960. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR000998. MAM_dom.
IPR011163. Pept_S1A_enterop.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF00629. MAM. 1 hit.
PF01390. SEA. 1 hit.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001138. Enteropeptidase. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 2 hits.
SM00137. MAM. 1 hit.
SM00200. SEA. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 2 hits.
PS50068. LDLRA_2. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50024. SEA. 1 hit.
PS00420. SRCR_1. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P98072-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSKRSVPSR HRSLTTYEVM FAVLFVILVA LCAGLIAVSW LSIQGSVKDA
60 70 80 90 100
AFGKSHEARG TLKIISGATY NPHLQDKLSV DFKVLAFDIQ QMIDDIFQSS
110 120 130 140 150
NLKNEYKNSR VLQFENGSII VIFDLLFDQW VSDKNVKEEL IQGIEANKSS
160 170 180 190 200
QLVTFHIDLN SIDITASLEN FSTISPATTS EKLTTSIPLA TPGNVSIECP
210 220 230 240 250
PDSRLCADAL KCIAIDLFCD GELNCPDGSD EDNKTCATAC DGRFLLTGSS
260 270 280 290 300
GSFEALHYPK PSNNTSAVCR WIIRVNQGLS IQLNFDYFNT YYADVLNIYE
310 320 330 340 350
GMGSSKILRA SLWSNNPGII RIFSNQVTAT FLIQSDESDY IGFKVTYTAF
360 370 380 390 400
NSKELNNYEK INCNFEDGFC FWIQDLNDDN EWERTQGSTF PPSTGPTFDH
410 420 430 440 450
TFGNESGFYI STPTGPGGRR ERVGLLTLPL DPTPEQACLS FWYYMYGENV
460 470 480 490 500
YKLSINISSD QNMEKTIFQK EGNYGQNWNY GQVTLNETVE FKVSFYGFKN
510 520 530 540 550
QILSDIALDD ISLTYGICNV SVYPEPTLVP TPPPELPTDC GGPHDLWEPN
560 570 580 590 600
TTFTSINFPN SYPNQAFCIW NLNAQKGKNI QLHFQEFDLE NIADVVEIRD
610 620 630 640 650
GEGDDSLFLA VYTGPGPVND VFSTTNRMTV LFITDNMLAK QGFKANFTTG
660 670 680 690 700
YGLGIPEPCK EDNFQCKDGE CIPLVNLCDG FPHCKDGSDE AHCVRLFNGT
710 720 730 740 750
TDSSGLVQFR IQSIWHVACA ENWTTQISDD VCQLLGLGTG NSSVPTFSTG
760 770 780 790 800
GGPYVNLNTA PNGSLILTPS QQCLEDSLIL LQCNYKSCGK KLVTQEVSPK
810 820 830 840 850
IVGGSDSREG AWPWVVALYF DDQQVCGASL VSRDWLVSAA HCVYGRNMEP
860 870 880 890 900
SKWKAVLGLH MASNLTSPQI ETRLIDQIVI NPHYNKRRKN NDIAMMHLEM
910 920 930 940 950
KVNYTDYIQP ICLPEENQVF PPGRICSIAG WGALIYQGST ADVLQEADVP
960 970 980 990 1000
LLSNEKCQQQ MPEYNITENM VCAGYEAGGV DSCQGDSGGP LMCQENNRWL
1010 1020 1030
LAGVTSFGYQ CALPNRPGVY ARVPRFTEWI QSFLH
Length:1,035
Mass (Da):114,887
Last modified:February 1, 1996 - v1
Checksum:iE207970B08296E13
GO
Isoform Short (identifier: P98072-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     166-192: Missing.

Show »
Length:1,008
Mass (Da):112,127
Checksum:iE987B6FAE94E9FF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti808R → Y AA sequence (PubMed:1799406).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005386166 – 192Missing in isoform Short. CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09859 mRNA. Translation: AAB40026.1.
L19663 mRNA. Translation: AAA16035.1.
PIRiA43090.
RefSeqiNP_776864.1. NM_174439.2. [P98072-1]
UniGeneiBt.66152.

Genome annotation databases

EnsembliENSBTAT00000000788; ENSBTAP00000000788; ENSBTAG00000000597. [P98072-1]
ENSBTAT00000064206; ENSBTAP00000054149; ENSBTAG00000000597. [P98072-2]
GeneIDi282009.
KEGGibta:282009.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09859 mRNA. Translation: AAB40026.1.
L19663 mRNA. Translation: AAA16035.1.
PIRiA43090.
RefSeqiNP_776864.1. NM_174439.2. [P98072-1]
UniGeneiBt.66152.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EKBX-ray2.30A788-800[»]
B801-1035[»]
ProteinModelPortaliP98072.
SMRiP98072.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000788.

Protein family/group databases

MEROPSiS01.156.

Proteomic databases

PaxDbiP98072.
PRIDEiP98072.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000000788; ENSBTAP00000000788; ENSBTAG00000000597. [P98072-1]
ENSBTAT00000064206; ENSBTAP00000054149; ENSBTAG00000000597. [P98072-2]
GeneIDi282009.
KEGGibta:282009.

Organism-specific databases

CTDi5651.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000112380.
HOVERGENiHBG005588.
InParanoidiP98072.
KOiK01316.
OMAiFEDGFCF.
OrthoDBiEOG091G0DF7.
TreeFamiTF351678.

Enzyme and pathway databases

BRENDAi3.4.21.9. 908.

Miscellaneous databases

EvolutionaryTraceiP98072.

Gene expression databases

BgeeiENSBTAG00000000597.

Family and domain databases

CDDicd00041. CUB. 2 hits.
cd06263. MAM. 1 hit.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
3.10.250.10. 1 hit.
3.30.70.960. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR000998. MAM_dom.
IPR011163. Pept_S1A_enterop.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF00629. MAM. 1 hit.
PF01390. SEA. 1 hit.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001138. Enteropeptidase. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 2 hits.
SM00137. MAM. 1 hit.
SM00200. SEA. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 2 hits.
PS50068. LDLRA_2. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50024. SEA. 1 hit.
PS00420. SRCR_1. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENTK_BOVIN
AccessioniPrimary (citable) accession number: P98072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.