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P98072

- ENTK_BOVIN

UniProt

P98072 - ENTK_BOVIN

Protein

Enteropeptidase

Gene

TMPRSS15

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

    Catalytic activityi

    Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei841 – 8411Charge relay system1 Publication
    Active sitei892 – 8921Charge relay system1 Publication
    Active sitei987 – 9871Charge relay system1 Publication

    GO - Molecular functioni

    1. scavenger receptor activity Source: InterPro
    2. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enteropeptidase (EC:3.4.21.9)
    Alternative name(s):
    Enterokinase
    Serine protease 7
    Transmembrane protease serine 15
    Cleaved into the following 2 chains:
    Gene namesi
    Name:TMPRSS15
    Synonyms:ENTK, PRSS7
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 1

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi889 – 8891K → A: Prevents the cleavage of trypsinogen. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedSequence Analysis
    Chaini2 – 800799Enteropeptidase non-catalytic heavy chainPRO_0000027717Add
    BLAST
    Chaini801 – 1035235Enteropeptidase catalytic light chainPRO_0000027718Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi199 ↔ 212By similarity
    Disulfide bondi206 ↔ 225By similarity
    Disulfide bondi219 ↔ 236By similarity
    Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi240 ↔ 269By similarity
    Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi404 – 4041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi486 – 4861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi519 – 5191N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi540 ↔ 568By similarity
    Glycosylationi550 – 5501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi646 – 6461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi659 ↔ 671By similarity
    Disulfide bondi666 ↔ 684By similarity
    Disulfide bondi678 ↔ 693By similarity
    Glycosylationi698 – 6981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi722 – 7221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi741 – 7411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi762 – 7621N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi773 ↔ 783By similarity
    Disulfide bondi788 ↔ 912Interchain (between heavy and light chains)
    Disulfide bondi826 ↔ 842By similarity
    Glycosylationi864 – 8641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi903 – 9031N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi926 ↔ 993By similarity
    Disulfide bondi957 ↔ 972By similarity
    Glycosylationi965 – 9651N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi983 ↔ 1011By similarity

    Post-translational modificationi

    The chains are derived from a single precursor that is cleaved by a trypsin-like protease.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Myristate, Zymogen

    Proteomic databases

    PRIDEiP98072.

    Expressioni

    Tissue specificityi

    Intestinal brush border.

    Interactioni

    Subunit structurei

    Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.1 Publication

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000000788.

    Structurei

    Secondary structure

    1
    1035
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi815 – 8206
    Beta strandi823 – 8308
    Beta strandi832 – 8387
    Helixi840 – 8434
    Helixi850 – 8523
    Beta strandi853 – 8586
    Beta strandi871 – 88010
    Turni886 – 8894
    Beta strandi894 – 9007
    Beta strandi925 – 93511
    Beta strandi945 – 9517
    Helixi954 – 9607
    Beta strandi970 – 9734
    Beta strandi990 – 9956
    Beta strandi998 – 100710
    Beta strandi1009 – 10124
    Beta strandi1018 – 10225
    Helixi1023 – 10253
    Helixi1027 – 10315

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EKBX-ray2.30A788-800[»]
    B801-1035[»]
    ProteinModelPortaliP98072.
    SMRiP98072. Positions 801-1035.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP98072.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1817CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini48 – 1035988ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 4729Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 169116SEAPROSITE-ProRule annotationAdd
    BLAST
    Domaini197 – 23842LDL-receptor class A 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini240 – 350111CUB 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini358 – 520163MAMPROSITE-ProRule annotationAdd
    BLAST
    Domaini540 – 650111CUB 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini657 – 69539LDL-receptor class A 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini694 – 78794SRCRPROSITE-ProRule annotationAdd
    BLAST
    Domaini801 – 1035235Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 2 CUB domains.PROSITE-ProRule annotation
    Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation
    Contains 1 MAM domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 1 SEA domain.PROSITE-ProRule annotation
    Contains 1 SRCR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00720000108393.
    HOGENOMiHOG000112380.
    HOVERGENiHBG005588.
    InParanoidiP98072.
    KOiK01316.
    OMAiFEDGFCF.
    OrthoDBiEOG7RNK07.
    TreeFamiTF351678.

    Family and domain databases

    Gene3Di2.60.120.290. 2 hits.
    3.10.250.10. 1 hit.
    3.30.70.960. 1 hit.
    4.10.400.10. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR000859. CUB_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR000998. MAM_dom.
    IPR011163. Pept_S1A_enterop.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000082. SEA_dom.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00431. CUB. 2 hits.
    PF00057. Ldl_recept_a. 1 hit.
    PF00629. MAM. 1 hit.
    PF01390. SEA. 1 hit.
    PF00530. SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001138. Enteropeptidase. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00042. CUB. 2 hits.
    SM00192. LDLa. 2 hits.
    SM00137. MAM. 1 hit.
    SM00200. SEA. 1 hit.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49854. SSF49854. 2 hits.
    SSF49899. SSF49899. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 2 hits.
    SSF82671. SSF82671. 1 hit.
    PROSITEiPS01180. CUB. 2 hits.
    PS01209. LDLRA_1. 2 hits.
    PS50068. LDLRA_2. 2 hits.
    PS00740. MAM_1. 1 hit.
    PS50060. MAM_2. 1 hit.
    PS50024. SEA. 1 hit.
    PS00420. SRCR_1. 1 hit.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P98072-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSKRSVPSR HRSLTTYEVM FAVLFVILVA LCAGLIAVSW LSIQGSVKDA     50
    AFGKSHEARG TLKIISGATY NPHLQDKLSV DFKVLAFDIQ QMIDDIFQSS 100
    NLKNEYKNSR VLQFENGSII VIFDLLFDQW VSDKNVKEEL IQGIEANKSS 150
    QLVTFHIDLN SIDITASLEN FSTISPATTS EKLTTSIPLA TPGNVSIECP 200
    PDSRLCADAL KCIAIDLFCD GELNCPDGSD EDNKTCATAC DGRFLLTGSS 250
    GSFEALHYPK PSNNTSAVCR WIIRVNQGLS IQLNFDYFNT YYADVLNIYE 300
    GMGSSKILRA SLWSNNPGII RIFSNQVTAT FLIQSDESDY IGFKVTYTAF 350
    NSKELNNYEK INCNFEDGFC FWIQDLNDDN EWERTQGSTF PPSTGPTFDH 400
    TFGNESGFYI STPTGPGGRR ERVGLLTLPL DPTPEQACLS FWYYMYGENV 450
    YKLSINISSD QNMEKTIFQK EGNYGQNWNY GQVTLNETVE FKVSFYGFKN 500
    QILSDIALDD ISLTYGICNV SVYPEPTLVP TPPPELPTDC GGPHDLWEPN 550
    TTFTSINFPN SYPNQAFCIW NLNAQKGKNI QLHFQEFDLE NIADVVEIRD 600
    GEGDDSLFLA VYTGPGPVND VFSTTNRMTV LFITDNMLAK QGFKANFTTG 650
    YGLGIPEPCK EDNFQCKDGE CIPLVNLCDG FPHCKDGSDE AHCVRLFNGT 700
    TDSSGLVQFR IQSIWHVACA ENWTTQISDD VCQLLGLGTG NSSVPTFSTG 750
    GGPYVNLNTA PNGSLILTPS QQCLEDSLIL LQCNYKSCGK KLVTQEVSPK 800
    IVGGSDSREG AWPWVVALYF DDQQVCGASL VSRDWLVSAA HCVYGRNMEP 850
    SKWKAVLGLH MASNLTSPQI ETRLIDQIVI NPHYNKRRKN NDIAMMHLEM 900
    KVNYTDYIQP ICLPEENQVF PPGRICSIAG WGALIYQGST ADVLQEADVP 950
    LLSNEKCQQQ MPEYNITENM VCAGYEAGGV DSCQGDSGGP LMCQENNRWL 1000
    LAGVTSFGYQ CALPNRPGVY ARVPRFTEWI QSFLH 1035
    Length:1,035
    Mass (Da):114,887
    Last modified:February 1, 1996 - v1
    Checksum:iE207970B08296E13
    GO
    Isoform Short (identifier: P98072-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         166-192: Missing.

    Show »
    Length:1,008
    Mass (Da):112,127
    Checksum:iE987B6FAE94E9FF9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti808 – 8081R → Y AA sequence (PubMed:1799406)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei166 – 19227Missing in isoform Short. CuratedVSP_005386Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09859 mRNA. Translation: AAB40026.1.
    L19663 mRNA. Translation: AAA16035.1.
    PIRiA43090.
    RefSeqiNP_776864.1. NM_174439.2. [P98072-1]
    UniGeneiBt.66152.

    Genome annotation databases

    EnsembliENSBTAT00000000788; ENSBTAP00000000788; ENSBTAG00000000597. [P98072-1]
    ENSBTAT00000064206; ENSBTAP00000054149; ENSBTAG00000000597. [P98072-2]
    GeneIDi282009.
    KEGGibta:282009.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09859 mRNA. Translation: AAB40026.1 .
    L19663 mRNA. Translation: AAA16035.1 .
    PIRi A43090.
    RefSeqi NP_776864.1. NM_174439.2. [P98072-1 ]
    UniGenei Bt.66152.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EKB X-ray 2.30 A 788-800 [» ]
    B 801-1035 [» ]
    ProteinModelPortali P98072.
    SMRi P98072. Positions 801-1035.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000000788.

    Protein family/group databases

    MEROPSi S01.156.

    Proteomic databases

    PRIDEi P98072.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000000788 ; ENSBTAP00000000788 ; ENSBTAG00000000597 . [P98072-1 ]
    ENSBTAT00000064206 ; ENSBTAP00000054149 ; ENSBTAG00000000597 . [P98072-2 ]
    GeneIDi 282009.
    KEGGi bta:282009.

    Organism-specific databases

    CTDi 5651.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00720000108393.
    HOGENOMi HOG000112380.
    HOVERGENi HBG005588.
    InParanoidi P98072.
    KOi K01316.
    OMAi FEDGFCF.
    OrthoDBi EOG7RNK07.
    TreeFami TF351678.

    Miscellaneous databases

    EvolutionaryTracei P98072.
    NextBioi 20805876.

    Family and domain databases

    Gene3Di 2.60.120.290. 2 hits.
    3.10.250.10. 1 hit.
    3.30.70.960. 1 hit.
    4.10.400.10. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR000859. CUB_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR000998. MAM_dom.
    IPR011163. Pept_S1A_enterop.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000082. SEA_dom.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00431. CUB. 2 hits.
    PF00057. Ldl_recept_a. 1 hit.
    PF00629. MAM. 1 hit.
    PF01390. SEA. 1 hit.
    PF00530. SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001138. Enteropeptidase. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00042. CUB. 2 hits.
    SM00192. LDLa. 2 hits.
    SM00137. MAM. 1 hit.
    SM00200. SEA. 1 hit.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49854. SSF49854. 2 hits.
    SSF49899. SSF49899. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 2 hits.
    SSF82671. SSF82671. 1 hit.
    PROSITEi PS01180. CUB. 2 hits.
    PS01209. LDLRA_1. 2 hits.
    PS50068. LDLRA_2. 2 hits.
    PS00740. MAM_1. 1 hit.
    PS50060. MAM_2. 1 hit.
    PS50024. SEA. 1 hit.
    PS00420. SRCR_1. 1 hit.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains."
      Kitamoto Y., Yuan X., Wu Q., McCourt D.W., Sadler J.E.
      Proc. Natl. Acad. Sci. U.S.A. 91:7588-7592(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Duodenum.
    2. "Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase."
      Lavallie E.R., Rehemtulla A., Racie L.A., Diblasio E.A., Ferenz C., Grant K.L., Light A., McCoy J.M.
      J. Biol. Chem. 268:23311-23317(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 801-1035, PARTIAL PROTEIN SEQUENCE.
    3. "The amino-terminal sequence of the catalytic subunit of bovine enterokinase."
      Light A., Janska H.
      J. Protein Chem. 10:475-480(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 801-827.
      Tissue: Intestine.
    4. "Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide."
      Lu D., Futterer K., Korolev S., Zheng X., Tan K., Waksman G., Sadler J.E.
      J. Mol. Biol. 292:361-373(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 788-1035 IN COMPLEX WITH INHIBITOR, ACTIVE SITE, SUBUNIT, INTERCHAIN DISULFIDE BOND, MUTAGENESIS OF LYS-889.

    Entry informationi

    Entry nameiENTK_BOVIN
    AccessioniPrimary (citable) accession number: P98072
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3