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Protein

Mannan-binding lectin serine protease 1

Gene

Masp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.1 Publication

Enzyme regulationi

Inhibited by SERPING1 and A2M.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Calcium 1By similarity1
Metal bindingi81Calcium 1By similarity1
Metal bindingi126Calcium 1By similarity1
Metal bindingi128Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi144Calcium 2By similarity1
Metal bindingi145Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi147Calcium 2By similarity1
Metal bindingi164Calcium 2By similarity1
Metal bindingi165Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi168Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi240Calcium 3By similarity1
Metal bindingi250Calcium 3By similarity1
Metal bindingi287Calcium 3By similarity1
Metal bindingi289Calcium 3; via carbonyl oxygenBy similarity1
Active sitei495Charge relay systemBy similarity1
Active sitei557Charge relay systemBy similarity1
Active sitei651Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • complement activation, lectin pathway Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement activation lectin pathway, Immunity, Innate immunity
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.B7 3474
ReactomeiR-MMU-166662 Lectin pathway of complement activation
R-MMU-166663 Initial triggering of complement
R-MMU-2855086 Ficolins bind to repetitive carbohydrate structures on the target cell surface
R-MMU-3000480 Scavenging by Class A Receptors

Protein family/group databases

MEROPSiS01.132

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan-binding lectin serine protease 1 (EC:3.4.21.-)
Alternative name(s):
Complement factor MASP-3
Complement-activating component of Ra-reactive factor
Mannose-binding lectin-associated serine protease 1
Short name:
MASP-1
Mannose-binding protein-associated serine protease
Ra-reactive factor serine protease p100
Short name:
RaRF
Serine protease 5
Cleaved into the following 2 chains:
Gene namesi
Name:Masp1
Synonyms:Crarf, Masp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:88492 Masp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mice are smaller and more vulnerable indicating developmental and growth defects. Mice serum has low C4 and C3 cleavage activity together with low MASP2 activation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
ChainiPRO_000002759525 – 704Mannan-binding lectin serine protease 1Add BLAST680
ChainiPRO_000002759625 – 453Mannan-binding lectin serine protease 1 heavy chainAdd BLAST429
ChainiPRO_0000027597454 – 704Mannan-binding lectin serine protease 1 light chainAdd BLAST251

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi78 ↔ 96By similarity
Disulfide bondi148 ↔ 162By similarity
Disulfide bondi158 ↔ 171By similarity
Modified residuei164(3R)-3-hydroxyasparagineSequence analysis1
Disulfide bondi173 ↔ 186By similarity
Glycosylationi183N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi190 ↔ 217By similarity
Disulfide bondi247 ↔ 265By similarity
Disulfide bondi306 ↔ 354By similarity
Disulfide bondi334 ↔ 367By similarity
Disulfide bondi372 ↔ 419By similarity
Glycosylationi390N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi402 ↔ 437By similarity
Glycosylationi412N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi441 ↔ 577Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi480 ↔ 496By similarity
Disulfide bondi619 ↔ 636By similarity
Disulfide bondi647 ↔ 677By similarity

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity
N-glycosylated. Some N-linked glycan are of the complex-type (By similarity).By similarity
Autoproteolytic processing of the proenzyme produces the active enzyme composed on the heavy and the light chain held together by a disulfide bond. Isoform 1 but not isoform 2 is activated through autoproteolytic processing (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei453 – 454Cleavage; by autolysisBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

MaxQBiP98064
PaxDbiP98064
PeptideAtlasiP98064
PRIDEiP98064

PTM databases

iPTMnetiP98064
PhosphoSitePlusiP98064

Expressioni

Tissue specificityi

Protein of the plasma which is primarily expressed by liver.2 Publications

Gene expression databases

BgeeiENSMUSG00000022887
CleanExiMM_MASP1

Interactioni

Subunit structurei

Homodimer. Interacts with the oligomeric lectins MBL1, MBL2, FCN2 and FCN3; triggers the lectin pathway of complement through activation of C3. Interacts with SERPING1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087327

Structurei

3D structure databases

ProteinModelPortaliP98064
SMRiP98064
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 143CUB 1PROSITE-ProRule annotationAdd BLAST119
Domaini144 – 187EGF-like; calcium-bindingBy similarityAdd BLAST44
Domaini190 – 302CUB 2PROSITE-ProRule annotationAdd BLAST113
Domaini304 – 369Sushi 1PROSITE-ProRule annotationAdd BLAST66
Domaini370 – 439Sushi 2PROSITE-ProRule annotationAdd BLAST70
Domaini454 – 701Peptidase S1PROSITE-ProRule annotationAdd BLAST248

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 305Interaction with MBL1By similarityAdd BLAST281
Regioni25 – 283Interaction with FCN2By similarityAdd BLAST259
Regioni25 – 189HomodimerizationBy similarityAdd BLAST165
Regioni25 – 189Interaction with MBL2By similarityAdd BLAST165

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118890
HOGENOMiHOG000237311
HOVERGENiHBG000559
InParanoidiP98064
KOiK03992
OMAiDLSQRWV
OrthoDBiEOG091G02DS
PhylomeDBiP98064
TreeFamiTF330373

Family and domain databases

CDDicd00033 CCP, 2 hits
cd00041 CUB, 2 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di2.60.120.290, 2 hits
InterProiView protein in InterPro
IPR000859 CUB_dom
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR018097 EGF_Ca-bd_CS
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR035914 Sperma_CUB_dom_sf
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF00084 Sushi, 2 hits
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00032 CCP, 2 hits
SM00042 CUB, 2 hits
SM00179 EGF_CA, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF50494 SSF50494, 1 hit
SSF57535 SSF57535, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS01180 CUB, 2 hits
PS01186 EGF_2, 1 hit
PS01187 EGF_CA, 1 hit
PS50923 SUSHI, 2 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P98064-1) [UniParc]FASTAAdd to basket
Also known as: MASP-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRFLSFWRLL LYHALCLALP EVSAHTVELN EMFGQIQSPG YPDSYPSDSE
60 70 80 90 100
VTWNITVPEG FRIKLYFMHF NLESSYLCEY DYVKVETEDQ VLATFCGRET
110 120 130 140 150
TDTEQTPGQE VVLSPGTFMS VTFRSDFSNE ERFTGFDAHY MAVDVDECKE
160 170 180 190 200
REDEELSCDH YCHNYIGGYY CSCRFGYILH TDNRTCRVEC SGNLFTQRTG
210 220 230 240 250
TITSPDYPNP YPKSSECSYT IDLEEGFMVS LQFEDIFDIE DHPEVPCPYD
260 270 280 290 300
YIKIKAGSKV WGPFCGEKSP EPISTQTHSV QILFRSDNSG ENRGWRLSYR
310 320 330 340 350
AAGNECPKLQ PPVYGKIEPS QAVYSFKDQV LVSCDTGYKV LKDNEVMDTF
360 370 380 390 400
QIECLKDGAW SNKIPTCKIV DCGAPAGLKH GLVTFSTRNN LTTYKSEIRY
410 420 430 440 450
SCQQPYYKML HNTTGVYTCS AHGTWTNEVL KRSLPTCLPV CGVPKFSRKQ
460 470 480 490 500
ISRIFNGRPA QKGTMPWIAM LSHLNGQPFC GGSLLGSNWV LTAAHCLHQS
510 520 530 540 550
LDPEEPTLHS SYLLSPSDFK IIMGKHWRRR SDEDEQHLHV KRTTLHPLYN
560 570 580 590 600
PSTFENDLGL VELSESPRLN DFVMPVCLPE QPSTEGTMVI VSGWGKQFLQ
610 620 630 640 650
RFPENLMEIE IPIVNSDTCQ EAYTPLKKKV TKDMICAGEK EGGKDACAGD
660 670 680 690 700
SGGPMVTKDA ERDQWYLVGV VSWGEDCGKK DRYGVYSYIY PNKDWIQRIT

GVRN
Length:704
Mass (Da):79,968
Last modified:April 14, 2009 - v2
Checksum:i4CF0B17916C10961
GO
Isoform 2 (identifier: P98064-2) [UniParc]FASTAAdd to basket
Also known as: MASP-3

The sequence of this isoform differs from the canonical sequence as follows:
     443-443: V → QPSRALPNLV...RAVRDLQVER
     444-704: Missing.

Note: Contains a N-linked (GlcNAc...) asparagine at position 538 Contains a N-linked (GlcNAc...) asparagine at position 604.By similarity
Show »
Length:733
Mass (Da):82,446
Checksum:i6B3B54D5D3F5822B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257G → A in AAI31638 (PubMed:15489334).Curated1
Sequence conflicti345E → G in BAA03944 (PubMed:8133044).Curated1
Sequence conflicti345E → G in BAB69688 (PubMed:12847554).Curated1
Sequence conflicti428E → K in BAA03944 (PubMed:8133044).Curated1
Sequence conflicti465M → T in AAN39850 (PubMed:12847554).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036814443V → QPSRALPNLVKRIIGGRNAE LGLFPWQALIVVEDTSRVPN DKWFGSGALLSESWILTAAH VLRSQRRDNTVIPVSKEHVT VYLGLHDVRDKSGAVNSSAA RVILHPDFNIQNYNHDIALV QLQKPVPLGAHVMPICLPRP EPEGPAPHMLGLVAGWGISN PNVTVDEIILSGTRTLSDVL QYVKLPVVSHAECKASYESR SGNYSVTENMFCAGYYEGGK DTCLGDSGGAFVIFDEMSQH WVAQGLVSWGGPEECGSKQV YGVYTKVSNYVDWLWEEMNS PRAVRDLQVER in isoform 2. 2 Publications1
Alternative sequenceiVSP_036815444 – 704Missing in isoform 2. 2 PublicationsAdd BLAST261

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16492 mRNA Translation: BAA03944.1
AB049755 mRNA Translation: BAB69688.1
AY135527, AY135525 Genomic DNA Translation: AAN39850.1
AK031598 mRNA Translation: BAC27469.1
CH466521 Genomic DNA Translation: EDK97670.1
CH466521 Genomic DNA Translation: EDK97671.1
BC131637 mRNA Translation: AAI31638.1
BC131638 mRNA Translation: AAI31639.1
CCDSiCCDS37303.1 [P98064-1]
PIRiPC1235
RefSeqiNP_032581.2, NM_008555.2 [P98064-1]
XP_006521891.1, XM_006521828.3
UniGeneiMm.1213

Genome annotation databases

EnsembliENSMUST00000089883; ENSMUSP00000087327; ENSMUSG00000022887 [P98064-1]
GeneIDi17174
KEGGimmu:17174
UCSCiuc007ytr.1 mouse [P98064-1]
uc007yts.1 mouse [P98064-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMASP1_MOUSE
AccessioniPrimary (citable) accession number: P98064
Secondary accession number(s): A2RRH8
, A2RRH9, Q8CD27, Q8CIR8, Q920S0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 14, 2009
Last modified: April 25, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health