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P98063

- BMP1_MOUSE

UniProt

P98063 - BMP1_MOUSE

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Protein

Bone morphogenetic protein 1

Gene

Bmp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD) By similarity. Responsible for the proteolytic activation of lysyl oxidase LOX.By similarity1 Publication

Catalytic activityi

Cleavage of the C-terminal propeptide at Ala-|-Asp in type I and II procollagens and at Arg-|-Asp in type III.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Activity is increased by the procollagen C-endopeptidase enhancer protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi218 – 2181Zinc; catalyticPROSITE-ProRule annotation
Active sitei219 – 2191PROSITE-ProRule annotation
Metal bindingi222 – 2221Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi228 – 2281Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: MGI
  3. peptidase activity Source: MGI
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. cartilage development Source: UniProtKB-KW
  2. cell differentiation Source: UniProtKB-KW
  3. ossification Source: UniProtKB-KW
  4. positive regulation of cartilage development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein, Growth factor, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Chondrogenesis, Differentiation, Osteogenesis

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_196515. Crosslinking of collagen fibrils.
REACT_196595. Anchoring fibril formation.
REACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199052. Degradation of the extracellular matrix.
REACT_213857. HDL-mediated lipid transport.

Protein family/group databases

MEROPSiM12.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Bone morphogenetic protein 1 (EC:3.4.24.19)
Short name:
BMP-1
Alternative name(s):
Mammalian tolloid protein
Short name:
mTld
Procollagen C-proteinase
Short name:
PCP
Gene namesi
Name:Bmp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:88176. Bmp1.

Subcellular locationi

Golgi apparatustrans-Golgi network 1 Publication. Secretedextracellular spaceextracellular matrix 1 Publication
Note: Co-localizes with POSTN in the Golgi.

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
  3. membrane-bounded vesicle Source: Ensembl
  4. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Golgi apparatus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 125100Sequence AnalysisPRO_0000028891Add
BLAST
Chaini126 – 991866Bone morphogenetic protein 1PRO_0000028892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi188 ↔ 191By similarity
Disulfide bondi327 ↔ 353By similarity
Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi380 ↔ 402By similarity
Disulfide bondi440 ↔ 466By similarity
Disulfide bondi493 ↔ 515By similarity
Disulfide bondi556 ↔ 568By similarity
Disulfide bondi564 ↔ 577By similarity
Disulfide bondi579 ↔ 592By similarity
Disulfide bondi596 ↔ 622By similarity
Glycosylationi604 – 6041N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi649 ↔ 671By similarity
Disulfide bondi712 ↔ 723By similarity
Disulfide bondi719 ↔ 732By similarity
Disulfide bondi734 ↔ 747By similarity
Disulfide bondi752 ↔ 778By similarity
Disulfide bondi805 ↔ 827By similarity
Disulfide bondi865 ↔ 895By similarity
Disulfide bondi922 ↔ 944By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP98063.
PRIDEiP98063.

PTM databases

PhosphoSiteiP98063.

Expressioni

Tissue specificityi

At high levels in embryonic maternal deciduum and floor plate region of the neural tube. Less in developing membranous and endochondral bone, submucosa of intestine, dermis of skin and the mesenchyme of spleen and lung.

Gene expression databases

CleanExiMM_BMP1.
ExpressionAtlasiP98063. baseline and differential.
GenevestigatoriP98063.

Interactioni

Subunit structurei

Interacts with POSTN, the interaction promotes deposition on the extracellular matrix.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP98063.
SMRiP98063. Positions 126-981.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini327 – 439113CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini440 – 551112CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini552 – 59342EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini596 – 707112CUB 3PROSITE-ProRule annotationAdd
BLAST
Domaini708 – 74841EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini752 – 864113CUB 4PROSITE-ProRule annotationAdd
BLAST
Domaini865 – 981117CUB 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 326201MetalloproteaseAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 5 CUB domains.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG70307.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiP98063.
KOiK05502.
OMAiGRFCGGK.
OrthoDBiEOG7N8ZTV.
TreeFamiTF314351.

Family and domain databases

Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
PF07645. EGF_CA. 1 hit.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P98063-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGVARPPLP LLSLPLLLLL LLLPRAGRPL DLADYTYDLG EEDAPELLNY
60 70 80 90 100
KDPCKAAAFL GDIALDEEDL RAFQVQQAAV LRQQTARRPS IKAAGNSSAL
110 120 130 140 150
GGQGTSGQPQ RESRGRWRGR PRSRRAATSR PERVWPDGVI PFVIGGNFTG
160 170 180 190 200
SQRAVFRQAM RHWEKHTCVT FLERTDEDSY IVFTYRPCGC CSYVGRRGGG
210 220 230 240 250
PQAISIGKNC DKFGIVVHEL GHVIGFWHEH TRPDRDRHVS IVRENIQPGQ
260 270 280 290 300
EYNFLKMEVQ EVESLGETYD FDSIMHYARN TFSRGIFLDT IVPKYEVNGV
310 320 330 340 350
KPSIGQRTRL SKGDIAQARK LYKCPACGET LQDSTGNFSS PEYPNGYSAH
360 370 380 390 400
MHCVWRISVT PGEKIILNFT SMDLYRSRLC WYDYVEVRDG FWRKAPLRGR
410 420 430 440 450
FCGGKLPEPI VSTDSRLWVE FRSSSNWVGK GFFAVYEAIC GGDVKKDNGH
460 470 480 490 500
IQSPNYPDDY RPSKVCIWRI QVSEGFHVGL TFQSFEIERH DSCAYDYLEV
510 520 530 540 550
RDGHSESSNL IGRYCGYEKP DDIKSTSSRL WLKFVSDGSI NKAGFAVNFF
560 570 580 590 600
KEVDECSRPN RGGCEQRCLN TLGSYKCSCD PGYELAPDKR RCEAACGGFL
610 620 630 640 650
TKLNGSITSP GWPKEYPPNK NCIWQLVAPT QYRISLQFDF FETEGNDVCK
660 670 680 690 700
YDFVEVRSGL TADSKLHGKF CGSEKPEVIT SQYNNMRVEF KSDNTVSKKG
710 720 730 740 750
FKAHFFSDKD ECSKDNGGCQ QDCVNTFGSY ECQCRSGFVL HDNKHDCKEA
760 770 780 790 800
GCEHKVTSTS GTITSPNWPD KYPSKKECTW AISSTPGHRV KLTFVEMDIE
810 820 830 840 850
SQPECAYDHL EVFDGRDAKA PVLGRFCGSK KPEPVLATGN RMFLRFYSDN
860 870 880 890 900
SVQRKGFQAS HSTECGGQVR ADVKTKDLYS HAQFGDNNYP GGVDCEWVIV
910 920 930 940 950
AEEGYGVELV FQTFEVEEET DCGYDYIELF DGYDSTAPRL GRYCGSGPPE
960 970 980 990
EVYSAGDSVL VKFHSDDTIS KKGFHLRYTS TKFQDTLHSR K
Length:991
Mass (Da):111,666
Last modified:October 3, 2012 - v2
Checksum:iF838441CF1DA9F1A
GO

Sequence cautioni

The sequence AAA37306.1 differs from that shown. Reason: Frameshift at positions 24 and 61.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3973APL → VWV in AAA37306. (PubMed:8174772)Curated
Sequence conflicti519 – 5191K → N in AAA37306. (PubMed:8174772)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24755 mRNA. Translation: AAA37306.1. Frameshift.
AC122268 Genomic DNA. No translation available.
BC066062 mRNA. Translation: AAH66062.1.
CCDSiCCDS36972.1.
PIRiI49540.
RefSeqiNP_033885.2. NM_009755.3.
XP_006518523.1. XM_006518460.1.
UniGeneiMm.27757.

Genome annotation databases

EnsembliENSMUST00000022693; ENSMUSP00000022693; ENSMUSG00000022098.
GeneIDi12153.
KEGGimmu:12153.
UCSCiuc007uoc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24755 mRNA. Translation: AAA37306.1 . Frameshift.
AC122268 Genomic DNA. No translation available.
BC066062 mRNA. Translation: AAH66062.1 .
CCDSi CCDS36972.1.
PIRi I49540.
RefSeqi NP_033885.2. NM_009755.3.
XP_006518523.1. XM_006518460.1.
UniGenei Mm.27757.

3D structure databases

ProteinModelPortali P98063.
SMRi P98063. Positions 126-981.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M12.005.

PTM databases

PhosphoSitei P98063.

Proteomic databases

PaxDbi P98063.
PRIDEi P98063.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022693 ; ENSMUSP00000022693 ; ENSMUSG00000022098 .
GeneIDi 12153.
KEGGi mmu:12153.
UCSCi uc007uoc.2. mouse.

Organism-specific databases

CTDi 649.
MGIi MGI:88176. Bmp1.

Phylogenomic databases

eggNOGi NOG70307.
GeneTreei ENSGT00760000119018.
HOGENOMi HOG000236339.
HOVERGENi HBG004859.
InParanoidi P98063.
KOi K05502.
OMAi GRFCGGK.
OrthoDBi EOG7N8ZTV.
TreeFami TF314351.

Enzyme and pathway databases

Reactomei REACT_196515. Crosslinking of collagen fibrils.
REACT_196595. Anchoring fibril formation.
REACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199052. Degradation of the extracellular matrix.
REACT_213857. HDL-mediated lipid transport.

Miscellaneous databases

NextBioi 280487.
PROi P98063.
SOURCEi Search...

Gene expression databases

CleanExi MM_BMP1.
ExpressionAtlasi P98063. baseline and differential.
Genevestigatori P98063.

Family and domain databases

Gene3Di 2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProi IPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view ]
Pfami PF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
PF07645. EGF_CA. 1 hit.
[Graphical view ]
PIRSFi PIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSi PR00480. ASTACIN.
SMARTi SM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF49854. SSF49854. 5 hits.
PROSITEi PS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Embryonic expression of mouse bone morphogenetic protein-1 (BMP-1), which is related to the Drosophila dorsoventral gene tolloid and encodes a putative astacin metalloendopeptidase."
    Fukagawa M., Noboru S., Hogan B.L.M., Jones C.M.
    Dev. Biol. 163:175-183(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Interaction between periostin and BMP-1 promotes proteolytic activation of lysyl oxidase."
    Maruhashi T., Kii I., Saito M., Kudo A.
    J. Biol. Chem. 285:13294-13303(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POSTN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiBMP1_MOUSE
AccessioniPrimary (citable) accession number: P98063
Secondary accession number(s): Q6NZM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 3, 2012
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3