Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P98063

- BMP1_MOUSE

UniProt

P98063 - BMP1_MOUSE

Protein

Bone morphogenetic protein 1

Gene

Bmp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD) By similarity. Responsible for the proteolytic activation of lysyl oxidase LOX.By similarity1 Publication

    Catalytic activityi

    Cleavage of the C-terminal propeptide at Ala-|-Asp in type I and II procollagens and at Arg-|-Asp in type III.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Enzyme regulationi

    Activity is increased by the procollagen C-endopeptidase enhancer protein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi218 – 2181Zinc; catalyticPROSITE-ProRule annotation
    Active sitei219 – 2191PROSITE-ProRule annotation
    Metal bindingi222 – 2221Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi228 – 2281Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. metalloendopeptidase activity Source: MGI
    3. peptidase activity Source: MGI
    4. protein binding Source: MGI
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cartilage development Source: UniProtKB-KW
    2. cell differentiation Source: UniProtKB-KW
    3. ossification Source: UniProtKB-KW
    4. positive regulation of cartilage development Source: MGI

    Keywords - Molecular functioni

    Cytokine, Developmental protein, Growth factor, Hydrolase, Metalloprotease, Protease

    Keywords - Biological processi

    Chondrogenesis, Differentiation, Osteogenesis

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_196515. Crosslinking of collagen fibrils.
    REACT_196595. Anchoring fibril formation.
    REACT_198984. Collagen biosynthesis and modifying enzymes.
    REACT_199052. Degradation of the extracellular matrix.
    REACT_213857. HDL-mediated lipid transport.

    Protein family/group databases

    MEROPSiM12.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bone morphogenetic protein 1 (EC:3.4.24.19)
    Short name:
    BMP-1
    Alternative name(s):
    Mammalian tolloid protein
    Short name:
    mTld
    Procollagen C-proteinase
    Short name:
    PCP
    Gene namesi
    Name:Bmp1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:88176. Bmp1.

    Subcellular locationi

    Golgi apparatustrans-Golgi network 1 Publication. Secretedextracellular spaceextracellular matrix 1 Publication
    Note: Co-localizes with POSTN in the Golgi.

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. membrane-bounded vesicle Source: Ensembl
    4. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Golgi apparatus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Propeptidei26 – 125100Sequence AnalysisPRO_0000028891Add
    BLAST
    Chaini126 – 991866Bone morphogenetic protein 1PRO_0000028892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi188 ↔ 191By similarity
    Disulfide bondi327 ↔ 353By similarity
    Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi380 ↔ 402By similarity
    Disulfide bondi440 ↔ 466By similarity
    Disulfide bondi493 ↔ 515By similarity
    Disulfide bondi556 ↔ 568By similarity
    Disulfide bondi564 ↔ 577By similarity
    Disulfide bondi579 ↔ 592By similarity
    Disulfide bondi596 ↔ 622By similarity
    Glycosylationi604 – 6041N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi649 ↔ 671By similarity
    Disulfide bondi712 ↔ 723By similarity
    Disulfide bondi719 ↔ 732By similarity
    Disulfide bondi734 ↔ 747By similarity
    Disulfide bondi752 ↔ 778By similarity
    Disulfide bondi805 ↔ 827By similarity
    Disulfide bondi865 ↔ 895By similarity
    Disulfide bondi922 ↔ 944By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP98063.
    PRIDEiP98063.

    PTM databases

    PhosphoSiteiP98063.

    Expressioni

    Tissue specificityi

    At high levels in embryonic maternal deciduum and floor plate region of the neural tube. Less in developing membranous and endochondral bone, submucosa of intestine, dermis of skin and the mesenchyme of spleen and lung.

    Gene expression databases

    ArrayExpressiP98063.
    CleanExiMM_BMP1.
    GenevestigatoriP98063.

    Interactioni

    Subunit structurei

    Interacts with POSTN, the interaction promotes deposition on the extracellular matrix.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP98063.
    SMRiP98063. Positions 126-981.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini327 – 439113CUB 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini440 – 551112CUB 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini552 – 59342EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini596 – 707112CUB 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini708 – 74841EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini752 – 864113CUB 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini865 – 981117CUB 5PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni126 – 326201MetalloproteaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M12A family.Curated
    Contains 5 CUB domains.PROSITE-ProRule annotation
    Contains 2 EGF-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG70307.
    GeneTreeiENSGT00750000117289.
    HOGENOMiHOG000236339.
    HOVERGENiHBG004859.
    KOiK05502.
    OMAiGRFCGGK.
    OrthoDBiEOG7N8ZTV.
    TreeFamiTF314351.

    Family and domain databases

    Gene3Di2.60.120.290. 5 hits.
    3.40.390.10. 1 hit.
    InterProiIPR015446. BMP_1/tolloid-like.
    IPR000859. CUB_dom.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001506. Peptidase_M12A.
    IPR006026. Peptidase_Metallo.
    [Graphical view]
    PfamiPF01400. Astacin. 1 hit.
    PF00431. CUB. 5 hits.
    PF07645. EGF_CA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
    PRINTSiPR00480. ASTACIN.
    SMARTiSM00042. CUB. 5 hits.
    SM00179. EGF_CA. 2 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49854. SSF49854. 5 hits.
    PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
    PS01180. CUB. 5 hits.
    PS01186. EGF_2. 2 hits.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 2 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P98063-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGVARPPLP LLSLPLLLLL LLLPRAGRPL DLADYTYDLG EEDAPELLNY    50
    KDPCKAAAFL GDIALDEEDL RAFQVQQAAV LRQQTARRPS IKAAGNSSAL 100
    GGQGTSGQPQ RESRGRWRGR PRSRRAATSR PERVWPDGVI PFVIGGNFTG 150
    SQRAVFRQAM RHWEKHTCVT FLERTDEDSY IVFTYRPCGC CSYVGRRGGG 200
    PQAISIGKNC DKFGIVVHEL GHVIGFWHEH TRPDRDRHVS IVRENIQPGQ 250
    EYNFLKMEVQ EVESLGETYD FDSIMHYARN TFSRGIFLDT IVPKYEVNGV 300
    KPSIGQRTRL SKGDIAQARK LYKCPACGET LQDSTGNFSS PEYPNGYSAH 350
    MHCVWRISVT PGEKIILNFT SMDLYRSRLC WYDYVEVRDG FWRKAPLRGR 400
    FCGGKLPEPI VSTDSRLWVE FRSSSNWVGK GFFAVYEAIC GGDVKKDNGH 450
    IQSPNYPDDY RPSKVCIWRI QVSEGFHVGL TFQSFEIERH DSCAYDYLEV 500
    RDGHSESSNL IGRYCGYEKP DDIKSTSSRL WLKFVSDGSI NKAGFAVNFF 550
    KEVDECSRPN RGGCEQRCLN TLGSYKCSCD PGYELAPDKR RCEAACGGFL 600
    TKLNGSITSP GWPKEYPPNK NCIWQLVAPT QYRISLQFDF FETEGNDVCK 650
    YDFVEVRSGL TADSKLHGKF CGSEKPEVIT SQYNNMRVEF KSDNTVSKKG 700
    FKAHFFSDKD ECSKDNGGCQ QDCVNTFGSY ECQCRSGFVL HDNKHDCKEA 750
    GCEHKVTSTS GTITSPNWPD KYPSKKECTW AISSTPGHRV KLTFVEMDIE 800
    SQPECAYDHL EVFDGRDAKA PVLGRFCGSK KPEPVLATGN RMFLRFYSDN 850
    SVQRKGFQAS HSTECGGQVR ADVKTKDLYS HAQFGDNNYP GGVDCEWVIV 900
    AEEGYGVELV FQTFEVEEET DCGYDYIELF DGYDSTAPRL GRYCGSGPPE 950
    EVYSAGDSVL VKFHSDDTIS KKGFHLRYTS TKFQDTLHSR K 991
    Length:991
    Mass (Da):111,666
    Last modified:October 3, 2012 - v2
    Checksum:iF838441CF1DA9F1A
    GO

    Sequence cautioni

    The sequence AAA37306.1 differs from that shown. Reason: Frameshift at positions 24 and 61.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti395 – 3973APL → VWV in AAA37306. (PubMed:8174772)Curated
    Sequence conflicti519 – 5191K → N in AAA37306. (PubMed:8174772)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24755 mRNA. Translation: AAA37306.1. Frameshift.
    AC122268 Genomic DNA. No translation available.
    BC066062 mRNA. Translation: AAH66062.1.
    CCDSiCCDS36972.1.
    PIRiI49540.
    RefSeqiNP_033885.2. NM_009755.3.
    XP_006518523.1. XM_006518460.1.
    UniGeneiMm.27757.

    Genome annotation databases

    EnsembliENSMUST00000022693; ENSMUSP00000022693; ENSMUSG00000022098.
    GeneIDi12153.
    KEGGimmu:12153.
    UCSCiuc007uoc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24755 mRNA. Translation: AAA37306.1 . Frameshift.
    AC122268 Genomic DNA. No translation available.
    BC066062 mRNA. Translation: AAH66062.1 .
    CCDSi CCDS36972.1.
    PIRi I49540.
    RefSeqi NP_033885.2. NM_009755.3.
    XP_006518523.1. XM_006518460.1.
    UniGenei Mm.27757.

    3D structure databases

    ProteinModelPortali P98063.
    SMRi P98063. Positions 126-981.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.005.

    PTM databases

    PhosphoSitei P98063.

    Proteomic databases

    PaxDbi P98063.
    PRIDEi P98063.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022693 ; ENSMUSP00000022693 ; ENSMUSG00000022098 .
    GeneIDi 12153.
    KEGGi mmu:12153.
    UCSCi uc007uoc.2. mouse.

    Organism-specific databases

    CTDi 649.
    MGIi MGI:88176. Bmp1.

    Phylogenomic databases

    eggNOGi NOG70307.
    GeneTreei ENSGT00750000117289.
    HOGENOMi HOG000236339.
    HOVERGENi HBG004859.
    KOi K05502.
    OMAi GRFCGGK.
    OrthoDBi EOG7N8ZTV.
    TreeFami TF314351.

    Enzyme and pathway databases

    Reactomei REACT_196515. Crosslinking of collagen fibrils.
    REACT_196595. Anchoring fibril formation.
    REACT_198984. Collagen biosynthesis and modifying enzymes.
    REACT_199052. Degradation of the extracellular matrix.
    REACT_213857. HDL-mediated lipid transport.

    Miscellaneous databases

    NextBioi 280487.
    PROi P98063.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P98063.
    CleanExi MM_BMP1.
    Genevestigatori P98063.

    Family and domain databases

    Gene3Di 2.60.120.290. 5 hits.
    3.40.390.10. 1 hit.
    InterProi IPR015446. BMP_1/tolloid-like.
    IPR000859. CUB_dom.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001506. Peptidase_M12A.
    IPR006026. Peptidase_Metallo.
    [Graphical view ]
    Pfami PF01400. Astacin. 1 hit.
    PF00431. CUB. 5 hits.
    PF07645. EGF_CA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001199. BMP_1/tolloid-like. 1 hit.
    PRINTSi PR00480. ASTACIN.
    SMARTi SM00042. CUB. 5 hits.
    SM00179. EGF_CA. 2 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49854. SSF49854. 5 hits.
    PROSITEi PS00010. ASX_HYDROXYL. 2 hits.
    PS01180. CUB. 5 hits.
    PS01186. EGF_2. 2 hits.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 2 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Embryonic expression of mouse bone morphogenetic protein-1 (BMP-1), which is related to the Drosophila dorsoventral gene tolloid and encodes a putative astacin metalloendopeptidase."
      Fukagawa M., Noboru S., Hogan B.L.M., Jones C.M.
      Dev. Biol. 163:175-183(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Embryo.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Interaction between periostin and BMP-1 promotes proteolytic activation of lysyl oxidase."
      Maruhashi T., Kii I., Saito M., Kudo A.
      J. Biol. Chem. 285:13294-13303(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH POSTN, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiBMP1_MOUSE
    AccessioniPrimary (citable) accession number: P98063
    Secondary accession number(s): Q6NZM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3