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Protein

Bone morphogenetic protein 1

Gene

Bmp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD) (By similarity). Responsible for the proteolytic activation of lysyl oxidase LOX.By similarity1 Publication

Catalytic activityi

Cleavage of the C-terminal propeptide at Ala-|-Asp in type I and II procollagens and at Arg-|-Asp in type III.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Activity is increased by the procollagen C-endopeptidase enhancer protein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi218 – 2181Zinc; catalyticPROSITE-ProRule annotation
Active sitei219 – 2191PROSITE-ProRule annotation
Metal bindingi222 – 2221Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi228 – 2281Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein, Growth factor, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Chondrogenesis, Differentiation, Osteogenesis

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_275864. Degradation of the extracellular matrix.
REACT_285754. Collagen biosynthesis and modifying enzymes.
REACT_299762. Crosslinking of collagen fibrils.
REACT_326379. HDL-mediated lipid transport.
REACT_343076. Anchoring fibril formation.

Protein family/group databases

MEROPSiM12.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Bone morphogenetic protein 1 (EC:3.4.24.19)
Short name:
BMP-1
Alternative name(s):
Mammalian tolloid protein
Short name:
mTld
Procollagen C-proteinase
Short name:
PCP
Gene namesi
Name:Bmp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:88176. Bmp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Golgi apparatus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 125100Sequence AnalysisPRO_0000028891Add
BLAST
Chaini126 – 991866Bone morphogenetic protein 1PRO_0000028892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi188 ↔ 191By similarity
Disulfide bondi327 ↔ 353By similarity
Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi380 ↔ 402By similarity
Disulfide bondi440 ↔ 466By similarity
Disulfide bondi493 ↔ 515By similarity
Disulfide bondi556 ↔ 568By similarity
Disulfide bondi564 ↔ 577By similarity
Disulfide bondi579 ↔ 592By similarity
Disulfide bondi596 ↔ 622By similarity
Glycosylationi604 – 6041N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi649 ↔ 671By similarity
Disulfide bondi712 ↔ 723By similarity
Disulfide bondi719 ↔ 732By similarity
Disulfide bondi734 ↔ 747By similarity
Disulfide bondi752 ↔ 778By similarity
Disulfide bondi805 ↔ 827By similarity
Disulfide bondi865 ↔ 895By similarity
Disulfide bondi922 ↔ 944By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP98063.
PRIDEiP98063.

PTM databases

PhosphoSiteiP98063.

Expressioni

Tissue specificityi

At high levels in embryonic maternal deciduum and floor plate region of the neural tube. Less in developing membranous and endochondral bone, submucosa of intestine, dermis of skin and the mesenchyme of spleen and lung.

Gene expression databases

CleanExiMM_BMP1.
ExpressionAtlasiP98063. baseline and differential.

Interactioni

Subunit structurei

Interacts with POSTN, the interaction promotes deposition on the extracellular matrix.1 Publication

Protein-protein interaction databases

BioGridi198360. 3 interactions.
STRINGi10090.ENSMUSP00000022693.

Structurei

3D structure databases

ProteinModelPortaliP98063.
SMRiP98063. Positions 126-981.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini327 – 439113CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini440 – 551112CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini552 – 59342EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini596 – 707112CUB 3PROSITE-ProRule annotationAdd
BLAST
Domaini708 – 74841EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini752 – 864113CUB 4PROSITE-ProRule annotationAdd
BLAST
Domaini865 – 981117CUB 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 326201MetalloproteaseAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 5 CUB domains.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG70307.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiP98063.
KOiK05502.
OMAiRGRFCGG.
OrthoDBiEOG7N8ZTV.
TreeFamiTF314351.

Family and domain databases

Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR029837. BMP-1.
IPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PANTHERiPTHR10127:SF305. PTHR10127:SF305. 1 hit.
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
PF07645. EGF_CA. 1 hit.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P98063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVARPPLP LLSLPLLLLL LLLPRAGRPL DLADYTYDLG EEDAPELLNY
60 70 80 90 100
KDPCKAAAFL GDIALDEEDL RAFQVQQAAV LRQQTARRPS IKAAGNSSAL
110 120 130 140 150
GGQGTSGQPQ RESRGRWRGR PRSRRAATSR PERVWPDGVI PFVIGGNFTG
160 170 180 190 200
SQRAVFRQAM RHWEKHTCVT FLERTDEDSY IVFTYRPCGC CSYVGRRGGG
210 220 230 240 250
PQAISIGKNC DKFGIVVHEL GHVIGFWHEH TRPDRDRHVS IVRENIQPGQ
260 270 280 290 300
EYNFLKMEVQ EVESLGETYD FDSIMHYARN TFSRGIFLDT IVPKYEVNGV
310 320 330 340 350
KPSIGQRTRL SKGDIAQARK LYKCPACGET LQDSTGNFSS PEYPNGYSAH
360 370 380 390 400
MHCVWRISVT PGEKIILNFT SMDLYRSRLC WYDYVEVRDG FWRKAPLRGR
410 420 430 440 450
FCGGKLPEPI VSTDSRLWVE FRSSSNWVGK GFFAVYEAIC GGDVKKDNGH
460 470 480 490 500
IQSPNYPDDY RPSKVCIWRI QVSEGFHVGL TFQSFEIERH DSCAYDYLEV
510 520 530 540 550
RDGHSESSNL IGRYCGYEKP DDIKSTSSRL WLKFVSDGSI NKAGFAVNFF
560 570 580 590 600
KEVDECSRPN RGGCEQRCLN TLGSYKCSCD PGYELAPDKR RCEAACGGFL
610 620 630 640 650
TKLNGSITSP GWPKEYPPNK NCIWQLVAPT QYRISLQFDF FETEGNDVCK
660 670 680 690 700
YDFVEVRSGL TADSKLHGKF CGSEKPEVIT SQYNNMRVEF KSDNTVSKKG
710 720 730 740 750
FKAHFFSDKD ECSKDNGGCQ QDCVNTFGSY ECQCRSGFVL HDNKHDCKEA
760 770 780 790 800
GCEHKVTSTS GTITSPNWPD KYPSKKECTW AISSTPGHRV KLTFVEMDIE
810 820 830 840 850
SQPECAYDHL EVFDGRDAKA PVLGRFCGSK KPEPVLATGN RMFLRFYSDN
860 870 880 890 900
SVQRKGFQAS HSTECGGQVR ADVKTKDLYS HAQFGDNNYP GGVDCEWVIV
910 920 930 940 950
AEEGYGVELV FQTFEVEEET DCGYDYIELF DGYDSTAPRL GRYCGSGPPE
960 970 980 990
EVYSAGDSVL VKFHSDDTIS KKGFHLRYTS TKFQDTLHSR K
Length:991
Mass (Da):111,666
Last modified:October 3, 2012 - v2
Checksum:iF838441CF1DA9F1A
GO

Sequence cautioni

The sequence AAA37306.1 differs from that shown. Reason: Frameshift at positions 24 and 61. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3973APL → VWV in AAA37306 (PubMed:8174772).Curated
Sequence conflicti519 – 5191K → N in AAA37306 (PubMed:8174772).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24755 mRNA. Translation: AAA37306.1. Frameshift.
AC122268 Genomic DNA. No translation available.
BC066062 mRNA. Translation: AAH66062.1.
CCDSiCCDS36972.1.
PIRiI49540.
RefSeqiNP_033885.2. NM_009755.3.
XP_006518523.1. XM_006518460.2.
UniGeneiMm.27757.

Genome annotation databases

EnsembliENSMUST00000022693; ENSMUSP00000022693; ENSMUSG00000022098.
GeneIDi12153.
KEGGimmu:12153.
UCSCiuc007uoc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24755 mRNA. Translation: AAA37306.1. Frameshift.
AC122268 Genomic DNA. No translation available.
BC066062 mRNA. Translation: AAH66062.1.
CCDSiCCDS36972.1.
PIRiI49540.
RefSeqiNP_033885.2. NM_009755.3.
XP_006518523.1. XM_006518460.2.
UniGeneiMm.27757.

3D structure databases

ProteinModelPortaliP98063.
SMRiP98063. Positions 126-981.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198360. 3 interactions.
STRINGi10090.ENSMUSP00000022693.

Protein family/group databases

MEROPSiM12.005.

PTM databases

PhosphoSiteiP98063.

Proteomic databases

PaxDbiP98063.
PRIDEiP98063.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022693; ENSMUSP00000022693; ENSMUSG00000022098.
GeneIDi12153.
KEGGimmu:12153.
UCSCiuc007uoc.2. mouse.

Organism-specific databases

CTDi649.
MGIiMGI:88176. Bmp1.

Phylogenomic databases

eggNOGiNOG70307.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiP98063.
KOiK05502.
OMAiRGRFCGG.
OrthoDBiEOG7N8ZTV.
TreeFamiTF314351.

Enzyme and pathway databases

ReactomeiREACT_275864. Degradation of the extracellular matrix.
REACT_285754. Collagen biosynthesis and modifying enzymes.
REACT_299762. Crosslinking of collagen fibrils.
REACT_326379. HDL-mediated lipid transport.
REACT_343076. Anchoring fibril formation.

Miscellaneous databases

NextBioi280487.
PROiP98063.
SOURCEiSearch...

Gene expression databases

CleanExiMM_BMP1.
ExpressionAtlasiP98063. baseline and differential.

Family and domain databases

Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR029837. BMP-1.
IPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PANTHERiPTHR10127:SF305. PTHR10127:SF305. 1 hit.
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
PF07645. EGF_CA. 1 hit.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Embryonic expression of mouse bone morphogenetic protein-1 (BMP-1), which is related to the Drosophila dorsoventral gene tolloid and encodes a putative astacin metalloendopeptidase."
    Fukagawa M., Noboru S., Hogan B.L.M., Jones C.M.
    Dev. Biol. 163:175-183(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Interaction between periostin and BMP-1 promotes proteolytic activation of lysyl oxidase."
    Maruhashi T., Kii I., Saito M., Kudo A.
    J. Biol. Chem. 285:13294-13303(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POSTN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiBMP1_MOUSE
AccessioniPrimary (citable) accession number: P98063
Secondary accession number(s): Q6NZM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 3, 2012
Last modified: June 24, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.