P98053 (COXM_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alternative cytochrome c oxidase subunit 2
EC=1.9.3.1

Alternative name(s):
Alternative cytochrome c oxidase polypeptide II
Cytochrome BB3 subunit 2
Oxidase BB(3) subunit 2

Gene names

Name:	coxM
Ordered Locus Names:	bll3785

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Developmental stage	Bacteroid (nitrogen-fixing endosymbiont).
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Copper Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Alternative cytochrome c oxidase subunit 2		PRO_0000183724
Regions
	Topological domain	1 – 40	40	Periplasmic Potential
	Transmembrane	41 – 61	21	Helical; Potential
	Topological domain	62 – 83	22	Cytoplasmic Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Topological domain	105 – 277	173	Periplasmic Potential
Sites
	Metal binding	190	1	Copper A Probable
	Metal binding	225	1	Copper A Probable
	Metal binding	229	1	Copper A Probable
	Metal binding	233	1	Copper A Probable

Sequences

	Sequence		Length	Mass (Da)
	P98053 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D262ADE2233D36E1	FASTA	277	31,327
	10 20 30 40 50 60 MAVALILLLI AIGSVLFHLF SPWWWTPIAT NWGYIDDTIN ITFWITGFVF TAVILFMAYC 70 80 90 100 110 120 VFRFHHKEGR QAAYNPENKK LEWWLSVGTG VGVAAMLAPG LVVWHQFVTV PADATEVEIM 130 140 150 160 170 180 GQQWQWSFRL PGKDGRLGTS DVRNISPENP MGLNRDDPHG QDDVVIENGD LHLPIGKPVK 190 200 210 220 230 240 VLLRSVDVLH DFYVPEFRAK MDMVPGMVTY FWIRPIRTGT FDVLCAELCG AAHYQMRAKV 250 260 270 IVEAESDYHA WLEQQKTFAG LSGRNAVVRA KYNSGDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes for a second terminal oxidase in Bradyrhizobium japonicum." Bott M., Preisig O., Hennecke H. Arch. Microbiol. 158:335-343(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68547 Genomic DNA. Translation: CAA48547.1. BA000040 Genomic DNA. Translation: BAC49050.1.
PIR	B48364.
RefSeq	NP_770425.1. NC_004463.1.
3D structure databases
ProteinModelPortal	P98053.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.bll3785.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC49050; BAC49050; BAC49050.
GeneID	1053041.
KEGG	bja:bll3785.
PATRIC	21191037. VBIBraJap65052_3776.
Phylogenomic databases
eggNOG	COG1622.
HOGENOM	HOG000268576.
KO	K02275.
OMA	ITFWITG.
ProtClustDB	CLSK2747381.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-3809-MONOMER.
Family and domain databases
Gene3D	1.10.287.90. 1 hit. 2.60.40.420. 1 hit.
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. [Graphical view]
Pfam	PF00116. COX2. 1 hit. [Graphical view]
SUPFAM	SSF49503. Cupredoxin. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COXM_BRAJA

Accession

Primary (citable) accession number: P98053

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program