Reviewed, UniProtKB/Swiss-Prot P97997 (MPPA_BLAEM)

Names and origin

Protein names	Recommended name:     Mitochondrial-processing peptidase subunit alpha     EC=3.4.24.64 Alternative name(s):     Alpha-MPP
Organism	Blastocladiella emersonii (Aquatic fungus)
Taxonomic identifier	4808 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Blastocladiomycota › Blastocladiomycetes › Blastocladiales › Blastocladiaceae › Blastocladiella

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Subunit structure	Heterodimer of alpha and beta subunits By similarity.
Subcellular location	Mitochondrion inner membrane. Ref.1
Sequence similarities	Belongs to the peptidase M16 family.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 474		Mitochondrial-processing peptidase subunit alpha		PRO_0000026770

Sequences

	Sequence		Length	Mass (Da)
	P97997-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 215D2252CC95D50E	FASTA	474	50,856
	10 20 30 40 50 60 MSPAAQLAKA AARSTCMTRL PSGIRVATAP SNSHFAAVGV YVDAGPIYET SIDRGVSHFV 70 80 90 100 110 120 SSLAFKSTHG ATESQVLKTM AGLGGNLFCT ATRESILYQG SVLHHDLPRT VQLLADTTLR 130 140 150 160 170 180 PALTEEEIAE RRATIAFEAE DLHSRPDAFI GEMMHAVAFG GRGLGNSIFC EPQRARNMTS 190 200 210 220 230 240 DTIREYFATY LHPSRMVVAG TGVAHAELVD LVSKAFVPSS TRAPSSVTHS DIETAYVGGS 250 260 270 280 290 300 HQLVIPKPPP THPNYEQTLT HVQVAFPVPP FTHPDMFPVS TLQVLMGGGG AFSAGGPGKG 310 320 330 340 350 360 MYSRLYTNVL NRYRWMESCA AFQHAYSSTS LFGISASCVP SFNPHLCNVL AGEFVHMARN 370 380 390 400 410 420 LSDEEVARAK NQLKSSLLMN LESQVITVED IGRQVLAQNQ RLEPLELVNN ISAVTRDDLV 430 440 450 460 470 RVAEALVAKP PTMVAVGEDL TKLTDIKETL AAFNASGEAL QPVGSAGSFG RVTM

References

[1]

"Characterization and submitochondrial localization of the alpha subunit of the mitochondrial processing peptidase from the aquatic fungus Blastocladiella emersonii." Rocha C.R., Gomes S.L. J. Bacteriol. 181:4257-4265(1999) [PubMed: 10400583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90568 Genomic DNA. Translation: AAB50243.1.
3D structure databases
HSSP	HSSP built from PDB template 1HR6 based on UniProtKB P11914.
ModBase	Search...
Protein family/group databases
MEROPS	M16.971.
Enzyme and pathway databases
BRENDA	3.4.24.64. 2001.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPA_BLAEM

Accession

Primary (citable) accession number: P97997

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)