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P97947 (NR0B2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 0 group B member 2
Alternative name(s):
Orphan nuclear receptor SHP
Small heterodimer partner
Gene names
Name:Nr0b2
Synonyms:Shp
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.

Subunit structure

Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Interacts with NR1I3 and EID1 By similarity. Interacts with RARA, RXRA, THRB, NR5A1, NR5A2, PPARA and PPARG. May also interact with HNF4A. Ref.1

Subcellular location

Cytoplasm By similarity. Nucleus Probable. Note: Colocalizes with NEUROD1 in the nucleus By similarity.

Tissue specificity

Detected in kidney, testis, heart and liver. Ref.1

Post-translational modification

Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR0 subfamily.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Molecular functionReceptor
Repressor
   PTMMethylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 15860571. Source: RGD

negative regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 15860571. Source: RGD

organ regeneration

Inferred from expression pattern PubMed 15904869. Source: RGD

positive regulation of insulin secretion

Inferred from mutant phenotype PubMed 17259388. Source: RGD

response to glucose

Inferred from mutant phenotype PubMed 17259388. Source: RGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

protein complex

Inferred from direct assay PubMed 10648597PubMed 15860571. Source: RGD

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

peroxisome proliferator activated receptor binding

Inferred from physical interaction Ref.1. Source: RGD

protein complex binding

Inferred from direct assay PubMed 10648597. Source: RGD

retinoic acid receptor binding

Inferred from physical interaction Ref.1. Source: RGD

retinoid X receptor binding

Inferred from physical interaction Ref.1. Source: RGD

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

thyroid hormone receptor binding

Inferred from physical interaction Ref.1. Source: RGD

transcription corepressor activity

Inferred from sequence or structural similarity. Source: UniProtKB

transcription factor binding

Inferred from physical interaction PubMed 15860571Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260Nuclear receptor subfamily 0 group B member 2
PRO_0000232757

Regions

Region60 – 231172Ligand-binding By similarity

Amino acid modifications

Modified residue571Symmetric dimethylarginine; by PRMT5 By similarity

Secondary structure

..... 260
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P97947 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 71C1E09C8B912A79

FASTA26028,750
        10         20         30         40         50         60 
MSSSQSGVCP CQGSASHPTI LYTLLSPGPR TRPMAPASRS HCLCQQHRPV RLCAPHRTCR 

        70         80         90        100        110        120 
EALDVLGKTV AFLRNLPSFC LLPHEDQRRL LEGCWGPLFL LGLAQDTVTF EVAEAPVPSI 

       130        140        150        160        170        180 
LKKILLEEPN SGAQGAQPPD PPQPSLAAVQ WLQHCLESFW SLELGPKEYA YLKGTILFNP 

       190        200        210        220        230        240 
DVPGLHASCH IAHLQQEAHW ALCEVLEPWY PASQGRLARI LLMASTLKNI SCTLLVDLFF 

       250        260 
RPVIGDVDIT ELLEDMLLLR 

« Hide

References

« Hide 'large scale' references
[1]"An orphan nuclear receptor lacking a zinc-finger DNA-binding domain: interaction with several nuclear receptors."
Masuda N., Yasumo H., Tamura T., Hashiguchi N., Furusawa T., Tsukamoto T., Sadano H., Osumi T.
Biochim. Biophys. Acta 1350:27-32(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH HNF4A; RARA; RXRA; PPARA AND THRB, TISSUE SPECIFICITY.
Strain: Fischer 344-Jcl and Sprague-Dawley.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[3]"Crystallographic identification and functional characterization of phospholipids as ligands for the orphan nuclear receptor steroidogenic factor-1."
Li Y., Choi M., Cavey G., Daugherty J., Suino K., Kovach A., Bingham N.C., Kliewer S.A., Xu H.E.
Mol. Cell 17:491-502(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 17-28 IN COMPLEX WITH NR5A1.
[4]"Structural and biochemical basis for selective repression of the orphan nuclear receptor liver receptor homolog 1 by small heterodimer partner."
Li Y., Choi M., Suino K., Kovach A., Daugherty J., Kliewer S.A., Xu H.E.
Proc. Natl. Acad. Sci. U.S.A. 102:9505-9510(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 16-26 IN COMPLEX WITH NR5A2 AND OF 115-131 IN COMPLEX WITH PPARG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86839 Genomic DNA. Translation: BAA13171.1.
D86580 mRNA. Translation: BAA13127.1.
BC088117 mRNA. Translation: AAH88117.1.
RefSeqNP_476474.1. NM_057133.1.
UniGeneRn.10712.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YP0X-ray1.50B17-28[»]
1ZGYX-ray1.80B115-131[»]
1ZH7X-ray2.50C/D16-26[»]
ProteinModelPortalP97947.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250721. 4 interactions.
DIPDIP-46314N.
STRING10116.ENSRNOP00000009683.

PTM databases

PhosphoSiteP97947.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000009683; ENSRNOP00000009683; ENSRNOG00000007229.
GeneID117274.
KEGGrno:117274.
UCSCRGD:621032. rat.

Organism-specific databases

CTD8431.
RGD621032. Nr0b2.

Phylogenomic databases

eggNOGNOG235713.
GeneTreeENSGT00390000015719.
HOGENOMHOG000231151.
HOVERGENHBG008136.
InParanoidP97947.
KOK08563.
OMAHIGHLQQ.
OrthoDBEOG7NSB35.
PhylomeDBP97947.
TreeFamTF332386.

Gene expression databases

GenevestigatorP97947.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
InterProIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
[Graphical view]
PRINTSPR00398. STRDHORMONER.
SMARTSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP97947.
NextBio620203.
PROP97947.

Entry information

Entry nameNR0B2_RAT
AccessionPrimary (citable) accession number: P97947
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: May 1, 1997
Last modified: May 14, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references