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P97947

- NR0B2_RAT

UniProt

P97947 - NR0B2_RAT

Protein

Nuclear receptor subfamily 0 group B member 2

Gene

Nr0b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. peroxisome proliferator activated receptor binding Source: RGD
    3. protein complex binding Source: RGD
    4. retinoic acid receptor binding Source: RGD
    5. retinoid X receptor binding Source: RGD
    6. sequence-specific DNA binding transcription factor activity Source: InterPro
    7. steroid hormone receptor activity Source: InterPro
    8. thyroid hormone receptor binding Source: RGD
    9. transcription corepressor activity Source: UniProtKB
    10. transcription factor binding Source: RGD

    GO - Biological processi

    1. negative regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
    2. negative regulation of transcription, DNA-templated Source: RGD
    3. negative regulation of transcription from RNA polymerase II promoter Source: RGD
    4. organ regeneration Source: RGD
    5. positive regulation of insulin secretion Source: RGD
    6. response to glucose Source: RGD
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Receptor, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 0 group B member 2
    Alternative name(s):
    Orphan nuclear receptor SHP
    Small heterodimer partner
    Gene namesi
    Name:Nr0b2
    Synonyms:Shp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi621032. Nr0b2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus Curated
    Note: Colocalizes with NEUROD1 in the nucleus.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. protein complex Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260Nuclear receptor subfamily 0 group B member 2PRO_0000232757Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei57 – 571Symmetric dimethylarginine; by PRMT5By similarity

    Post-translational modificationi

    Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.

    Keywords - PTMi

    Methylation

    PTM databases

    PhosphoSiteiP97947.

    Expressioni

    Tissue specificityi

    Detected in kidney, testis, heart and liver.1 Publication

    Gene expression databases

    GenevestigatoriP97947.

    Interactioni

    Subunit structurei

    Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Interacts with NR1I3 and EID1 By similarity. Interacts with RARA, RXRA, THRB, NR5A1, NR5A2, PPARA and PPARG. May also interact with HNF4A.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi250721. 4 interactions.
    DIPiDIP-46314N.
    STRINGi10116.ENSRNOP00000009683.

    Structurei

    Secondary structure

    1
    260
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi20 – 256
    Helixi120 – 1267

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YP0X-ray1.50B17-28[»]
    1ZGYX-ray1.80B115-131[»]
    1ZH7X-ray2.50C/D16-26[»]
    ProteinModelPortaliP97947.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP97947.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni60 – 231172Ligand-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG235713.
    GeneTreeiENSGT00390000015719.
    HOGENOMiHOG000231151.
    HOVERGENiHBG008136.
    InParanoidiP97947.
    KOiK08563.
    OMAiHIGHLQQ.
    OrthoDBiEOG7NSB35.
    PhylomeDBiP97947.
    TreeFamiTF332386.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    [Graphical view]
    PRINTSiPR00398. STRDHORMONER.
    SMARTiSM00430. HOLI. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P97947-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSQSGVCP CQGSASHPTI LYTLLSPGPR TRPMAPASRS HCLCQQHRPV    50
    RLCAPHRTCR EALDVLGKTV AFLRNLPSFC LLPHEDQRRL LEGCWGPLFL 100
    LGLAQDTVTF EVAEAPVPSI LKKILLEEPN SGAQGAQPPD PPQPSLAAVQ 150
    WLQHCLESFW SLELGPKEYA YLKGTILFNP DVPGLHASCH IAHLQQEAHW 200
    ALCEVLEPWY PASQGRLARI LLMASTLKNI SCTLLVDLFF RPVIGDVDIT 250
    ELLEDMLLLR 260
    Length:260
    Mass (Da):28,750
    Last modified:May 1, 1997 - v1
    Checksum:i71C1E09C8B912A79
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86839 Genomic DNA. Translation: BAA13171.1.
    D86580 mRNA. Translation: BAA13127.1.
    BC088117 mRNA. Translation: AAH88117.1.
    RefSeqiNP_476474.1. NM_057133.1.
    UniGeneiRn.10712.

    Genome annotation databases

    EnsembliENSRNOT00000009683; ENSRNOP00000009683; ENSRNOG00000007229.
    GeneIDi117274.
    KEGGirno:117274.
    UCSCiRGD:621032. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86839 Genomic DNA. Translation: BAA13171.1 .
    D86580 mRNA. Translation: BAA13127.1 .
    BC088117 mRNA. Translation: AAH88117.1 .
    RefSeqi NP_476474.1. NM_057133.1.
    UniGenei Rn.10712.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YP0 X-ray 1.50 B 17-28 [» ]
    1ZGY X-ray 1.80 B 115-131 [» ]
    1ZH7 X-ray 2.50 C/D 16-26 [» ]
    ProteinModelPortali P97947.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250721. 4 interactions.
    DIPi DIP-46314N.
    STRINGi 10116.ENSRNOP00000009683.

    PTM databases

    PhosphoSitei P97947.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000009683 ; ENSRNOP00000009683 ; ENSRNOG00000007229 .
    GeneIDi 117274.
    KEGGi rno:117274.
    UCSCi RGD:621032. rat.

    Organism-specific databases

    CTDi 8431.
    RGDi 621032. Nr0b2.

    Phylogenomic databases

    eggNOGi NOG235713.
    GeneTreei ENSGT00390000015719.
    HOGENOMi HOG000231151.
    HOVERGENi HBG008136.
    InParanoidi P97947.
    KOi K08563.
    OMAi HIGHLQQ.
    OrthoDBi EOG7NSB35.
    PhylomeDBi P97947.
    TreeFami TF332386.

    Miscellaneous databases

    EvolutionaryTracei P97947.
    NextBioi 620203.
    PROi P97947.

    Gene expression databases

    Genevestigatori P97947.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    [Graphical view ]
    PRINTSi PR00398. STRDHORMONER.
    SMARTi SM00430. HOLI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "An orphan nuclear receptor lacking a zinc-finger DNA-binding domain: interaction with several nuclear receptors."
      Masuda N., Yasumo H., Tamura T., Hashiguchi N., Furusawa T., Tsukamoto T., Sadano H., Osumi T.
      Biochim. Biophys. Acta 1350:27-32(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH HNF4A; RARA; RXRA; PPARA AND THRB, TISSUE SPECIFICITY.
      Strain: Fischer 344-Jcl and Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    3. "Crystallographic identification and functional characterization of phospholipids as ligands for the orphan nuclear receptor steroidogenic factor-1."
      Li Y., Choi M., Cavey G., Daugherty J., Suino K., Kovach A., Bingham N.C., Kliewer S.A., Xu H.E.
      Mol. Cell 17:491-502(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 17-28 IN COMPLEX WITH NR5A1.
    4. "Structural and biochemical basis for selective repression of the orphan nuclear receptor liver receptor homolog 1 by small heterodimer partner."
      Li Y., Choi M., Suino K., Kovach A., Daugherty J., Kliewer S.A., Xu H.E.
      Proc. Natl. Acad. Sci. U.S.A. 102:9505-9510(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 16-26 IN COMPLEX WITH NR5A2 AND OF 115-131 IN COMPLEX WITH PPARG.

    Entry informationi

    Entry nameiNR0B2_RAT
    AccessioniPrimary (citable) accession number: P97947
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3