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P97947

- NR0B2_RAT

UniProt

P97947 - NR0B2_RAT

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Protein

Nuclear receptor subfamily 0 group B member 2

Gene

Nr0b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. peroxisome proliferator activated receptor binding Source: RGD
  3. protein complex binding Source: RGD
  4. retinoic acid receptor binding Source: RGD
  5. retinoid X receptor binding Source: RGD
  6. sequence-specific DNA binding transcription factor activity Source: InterPro
  7. steroid hormone receptor activity Source: InterPro
  8. thyroid hormone receptor binding Source: RGD
  9. transcription corepressor activity Source: UniProtKB
  10. transcription factor binding Source: RGD

GO - Biological processi

  1. negative regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
  2. negative regulation of transcription, DNA-templated Source: RGD
  3. negative regulation of transcription from RNA polymerase II promoter Source: RGD
  4. organ regeneration Source: RGD
  5. positive regulation of insulin secretion Source: RGD
  6. response to glucose Source: RGD
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_242509. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 0 group B member 2
Alternative name(s):
Orphan nuclear receptor SHP
Small heterodimer partner
Gene namesi
Name:Nr0b2
Synonyms:Shp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi621032. Nr0b2.

Subcellular locationi

Cytoplasm By similarity. Nucleus Curated
Note: Colocalizes with NEUROD1 in the nucleus.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Nuclear receptor subfamily 0 group B member 2PRO_0000232757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571Symmetric dimethylarginine; by PRMT5By similarity

Post-translational modificationi

Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.

Keywords - PTMi

Methylation

PTM databases

PhosphoSiteiP97947.

Expressioni

Tissue specificityi

Detected in kidney, testis, heart and liver.1 Publication

Gene expression databases

GenevestigatoriP97947.

Interactioni

Subunit structurei

Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Interacts with NR1I3 and EID1 (By similarity). Interacts with RARA, RXRA, THRB, NR5A1, NR5A2, PPARA and PPARG. May also interact with HNF4A.By similarity3 Publications

Protein-protein interaction databases

BioGridi250721. 4 interactions.
DIPiDIP-46314N.
STRINGi10116.ENSRNOP00000009683.

Structurei

Secondary structure

1
260
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 256Combined sources
Helixi120 – 1267Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YP0X-ray1.50B17-28[»]
1ZGYX-ray1.80B115-131[»]
1ZH7X-ray2.50C/D16-26[»]
ProteinModelPortaliP97947.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP97947.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 231172Ligand-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG235713.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG008136.
InParanoidiP97947.
KOiK08563.
OMAiHIGHLQQ.
OrthoDBiEOG7NSB35.
PhylomeDBiP97947.
TreeFamiTF332386.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.

Sequencei

Sequence statusi: Complete.

P97947-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSQSGVCP CQGSASHPTI LYTLLSPGPR TRPMAPASRS HCLCQQHRPV
60 70 80 90 100
RLCAPHRTCR EALDVLGKTV AFLRNLPSFC LLPHEDQRRL LEGCWGPLFL
110 120 130 140 150
LGLAQDTVTF EVAEAPVPSI LKKILLEEPN SGAQGAQPPD PPQPSLAAVQ
160 170 180 190 200
WLQHCLESFW SLELGPKEYA YLKGTILFNP DVPGLHASCH IAHLQQEAHW
210 220 230 240 250
ALCEVLEPWY PASQGRLARI LLMASTLKNI SCTLLVDLFF RPVIGDVDIT
260
ELLEDMLLLR
Length:260
Mass (Da):28,750
Last modified:May 1, 1997 - v1
Checksum:i71C1E09C8B912A79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86839 Genomic DNA. Translation: BAA13171.1.
D86580 mRNA. Translation: BAA13127.1.
BC088117 mRNA. Translation: AAH88117.1.
RefSeqiNP_476474.1. NM_057133.1.
UniGeneiRn.10712.

Genome annotation databases

EnsembliENSRNOT00000009683; ENSRNOP00000009683; ENSRNOG00000007229.
GeneIDi117274.
KEGGirno:117274.
UCSCiRGD:621032. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86839 Genomic DNA. Translation: BAA13171.1 .
D86580 mRNA. Translation: BAA13127.1 .
BC088117 mRNA. Translation: AAH88117.1 .
RefSeqi NP_476474.1. NM_057133.1.
UniGenei Rn.10712.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1YP0 X-ray 1.50 B 17-28 [» ]
1ZGY X-ray 1.80 B 115-131 [» ]
1ZH7 X-ray 2.50 C/D 16-26 [» ]
ProteinModelPortali P97947.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250721. 4 interactions.
DIPi DIP-46314N.
STRINGi 10116.ENSRNOP00000009683.

PTM databases

PhosphoSitei P97947.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000009683 ; ENSRNOP00000009683 ; ENSRNOG00000007229 .
GeneIDi 117274.
KEGGi rno:117274.
UCSCi RGD:621032. rat.

Organism-specific databases

CTDi 8431.
RGDi 621032. Nr0b2.

Phylogenomic databases

eggNOGi NOG235713.
GeneTreei ENSGT00390000015719.
HOGENOMi HOG000231151.
HOVERGENi HBG008136.
InParanoidi P97947.
KOi K08563.
OMAi HIGHLQQ.
OrthoDBi EOG7NSB35.
PhylomeDBi P97947.
TreeFami TF332386.

Enzyme and pathway databases

Reactomei REACT_242509. Nuclear Receptor transcription pathway.

Miscellaneous databases

EvolutionaryTracei P97947.
NextBioi 620203.
PROi P97947.

Gene expression databases

Genevestigatori P97947.

Family and domain databases

Gene3Di 1.10.565.10. 1 hit.
InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
[Graphical view ]
PRINTSi PR00398. STRDHORMONER.
SMARTi SM00430. HOLI. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An orphan nuclear receptor lacking a zinc-finger DNA-binding domain: interaction with several nuclear receptors."
    Masuda N., Yasumo H., Tamura T., Hashiguchi N., Furusawa T., Tsukamoto T., Sadano H., Osumi T.
    Biochim. Biophys. Acta 1350:27-32(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH HNF4A; RARA; RXRA; PPARA AND THRB, TISSUE SPECIFICITY.
    Strain: Fischer 344-Jcl and Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  3. "Crystallographic identification and functional characterization of phospholipids as ligands for the orphan nuclear receptor steroidogenic factor-1."
    Li Y., Choi M., Cavey G., Daugherty J., Suino K., Kovach A., Bingham N.C., Kliewer S.A., Xu H.E.
    Mol. Cell 17:491-502(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 17-28 IN COMPLEX WITH NR5A1.
  4. "Structural and biochemical basis for selective repression of the orphan nuclear receptor liver receptor homolog 1 by small heterodimer partner."
    Li Y., Choi M., Suino K., Kovach A., Daugherty J., Kliewer S.A., Xu H.E.
    Proc. Natl. Acad. Sci. U.S.A. 102:9505-9510(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 16-26 IN COMPLEX WITH NR5A2 AND OF 115-131 IN COMPLEX WITH PPARG.

Entry informationi

Entry nameiNR0B2_RAT
AccessioniPrimary (citable) accession number: P97947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: May 1, 1997
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3