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Protein

Thymidylate kinase

Gene

Dtymk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of dTMP to dTDP.

Catalytic activityi

ATP + dTMP = ADP + dTDP.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 208ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. nucleoside phosphate kinase activity Source: MGI
  3. thymidylate kinase activity Source: MGI
  4. uridylate kinase activity Source: GO_Central

GO - Biological processi

  1. cellular response to growth factor stimulus Source: MGI
  2. dTDP biosynthetic process Source: MGI
  3. dTTP biosynthetic process Source: GO_Central
  4. dUDP biosynthetic process Source: GO_Central
  5. myoblast differentiation Source: Ensembl
  6. nucleoside monophosphate phosphorylation Source: MGI
  7. nucleotide biosynthetic process Source: MGI
  8. nucleotide phosphorylation Source: MGI
  9. response to cadmium ion Source: Ensembl
  10. response to estrogen Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_238368. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP97930.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate kinase (EC:2.7.4.9)
Alternative name(s):
dTMP kinase
Gene namesi
Name:Dtymk
Synonyms:Tmk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:108396. Dtymk.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
  2. mitochondrial intermembrane space Source: Ensembl
  3. mitochondrial matrix Source: Ensembl
  4. mitochondrion Source: GO_Central
  5. nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 212211Thymidylate kinasePRO_0000155211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP97930.
PaxDbiP97930.
PRIDEiP97930.

PTM databases

PhosphoSiteiP97930.

Expressioni

Gene expression databases

BgeeiP97930.
CleanExiMM_DTYMK.
ExpressionAtlasiP97930. baseline and differential.
GenevestigatoriP97930.

Interactioni

Protein-protein interaction databases

BioGridi204232. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP97930.
SMRiP97930. Positions 4-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0125.
GeneTreeiENSGT00390000017546.
HOGENOMiHOG000229079.
HOVERGENiHBG051418.
InParanoidiP97930.
KOiK00943.
OMAiMIDSYLR.
OrthoDBiEOG7WQ7T8.
TreeFamiTF324638.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00165. Thymidylate_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P97930-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRRGALIV LEGVDRAGKT TQGLKLVTAL CASGHRAELL RFPERSTEIG
60 70 80 90 100
KLLNSYLEKK TELEDHSVHL LFSANRWEQV PLIKAKLNQG VTLVLDRYAF
110 120 130 140 150
SGVAFTGAKE NFSLDWCKQP DVGLPKPDLI LFLQLQLLDA AARGEFGLER
160 170 180 190 200
YETGTFQKQV LLCFQQLMEE KNLNWKVVDA SKSIEEVHKE IRAHSEDAIR
210
NAAQRPLGEL WK
Length:212
Mass (Da):23,915
Last modified:July 1, 2008 - v2
Checksum:i2F9BC1770DE5902B
GO
Isoform Short (identifier: P97930-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-212: Missing.

Show »
Length:80
Mass (Da):8,947
Checksum:iE4A9A7E19995D02D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511Y → N in BAC40343. (PubMed:16141072)Curated
Sequence conflicti183 – 21230SIEEV…GELWK → RTPSETLHRGHWGSYGNKSA SIANTIFWFCKRLVEGSHLY TISRS in AAB46838. (PubMed:8845311)CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei81 – 212132Missing in isoform Short. 1 PublicationVSP_003029Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82852 mRNA. Translation: AAB46837.1.
S82853 mRNA. Translation: AAB46838.1.
AK088416 mRNA. Translation: BAC40343.1.
BC030178 mRNA. Translation: AAH30178.1.
CCDSiCCDS35672.1. [P97930-2]
CCDS48329.1. [P97930-1]
RefSeqiNP_001099137.1. NM_001105667.1. [P97930-1]
UniGeneiMm.250332.

Genome annotation databases

EnsembliENSMUST00000027503; ENSMUSP00000027503; ENSMUSG00000026281. [P97930-1]
ENSMUST00000112890; ENSMUSP00000108511; ENSMUSG00000026281. [P97930-2]
GeneIDi21915.
KEGGimmu:21915.
UCSCiuc007cek.2. mouse. [P97930-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82852 mRNA. Translation: AAB46837.1.
S82853 mRNA. Translation: AAB46838.1.
AK088416 mRNA. Translation: BAC40343.1.
BC030178 mRNA. Translation: AAH30178.1.
CCDSiCCDS35672.1. [P97930-2]
CCDS48329.1. [P97930-1]
RefSeqiNP_001099137.1. NM_001105667.1. [P97930-1]
UniGeneiMm.250332.

3D structure databases

ProteinModelPortaliP97930.
SMRiP97930. Positions 4-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204232. 1 interaction.

PTM databases

PhosphoSiteiP97930.

Proteomic databases

MaxQBiP97930.
PaxDbiP97930.
PRIDEiP97930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027503; ENSMUSP00000027503; ENSMUSG00000026281. [P97930-1]
ENSMUST00000112890; ENSMUSP00000108511; ENSMUSG00000026281. [P97930-2]
GeneIDi21915.
KEGGimmu:21915.
UCSCiuc007cek.2. mouse. [P97930-1]

Organism-specific databases

CTDi1841.
MGIiMGI:108396. Dtymk.

Phylogenomic databases

eggNOGiCOG0125.
GeneTreeiENSGT00390000017546.
HOGENOMiHOG000229079.
HOVERGENiHBG051418.
InParanoidiP97930.
KOiK00943.
OMAiMIDSYLR.
OrthoDBiEOG7WQ7T8.
TreeFamiTF324638.

Enzyme and pathway databases

UniPathwayiUPA00575.
ReactomeiREACT_238368. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP97930.

Miscellaneous databases

NextBioi301468.
PROiP97930.
SOURCEiSearch...

Gene expression databases

BgeeiP97930.
CleanExiMM_DTYMK.
ExpressionAtlasiP97930. baseline and differential.
GenevestigatoriP97930.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00165. Thymidylate_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the murine thymidylate kinase gene."
    Liang P., Averboukh L., Zhu W., Haley T., Pardee A.B.
    Cell Growth Differ. 6:1333-1338(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Strain: BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: NOD.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: Czech II.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiKTHY_MOUSE
AccessioniPrimary (citable) accession number: P97930
Secondary accession number(s): Q8C2L0, Q8K2S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 2008
Last modified: February 4, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.