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P97927

- LAMA4_MOUSE

UniProt

P97927 - LAMA4_MOUSE

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Protein
Laminin subunit alpha-4
Gene
Lama4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

GO - Biological processi

  1. blood vessel development Source: MGI
  2. brown fat cell differentiation Source: MGI
  3. cell adhesion Source: UniProtKB-KW
  4. regulation of cell adhesion Source: InterPro
  5. regulation of cell migration Source: InterPro
  6. regulation of embryonic development Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_196606. ECM proteoglycans.
REACT_196607. Non-integrin membrane-ECM interactions.
REACT_202342. Laminin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Laminin subunit alpha-4
Alternative name(s):
Laminin-14 subunit alpha
Laminin-8 subunit alpha
Laminin-9 subunit alpha
Gene namesi
Name:Lama4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:109321. Lama4.

Subcellular locationi

GO - Cellular componenti

  1. basal lamina Source: MGI
  2. basement membrane Source: MGI
  3. extracellular region Source: Reactome
  4. extracellular vesicular exosome Source: Ensembl
  5. laminin-1 complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 18161792Laminin subunit alpha-4
PRO_0000017061Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi82 ↔ 91 By similarity
Disulfide bondi84 ↔ 98 By similarity
Disulfide bondi101 ↔ 110 By similarity
Glycosylationi104 – 1041N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi113 ↔ 129 By similarity
Disulfide bondi132 ↔ 146 By similarity
Disulfide bondi134 ↔ 155 By similarity
Disulfide bondi157 ↔ 166 By similarity
Disulfide bondi169 ↔ 184 By similarity
Disulfide bondi187 ↔ 202 By similarity
Disulfide bondi189 ↔ 209 By similarity
Disulfide bondi212 ↔ 221 By similarity
Glycosylationi215 – 2151N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi224 ↔ 238 By similarity
Disulfide bondi266 – 266Interchain Inferred
Disulfide bondi269 – 269Interchain Inferred
Glycosylationi308 – 3081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi333 – 3331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi458 – 4581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi550 – 5501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi571 – 5711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi574 – 5741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi631 – 6311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi639 – 6391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi735 – 7351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi751 – 7511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi754 – 7541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi780 – 7801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi803 – 8031N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi1000 ↔ 1030 By similarity
Glycosylationi1088 – 10881N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi1196 ↔ 1222 By similarity
Glycosylationi1283 – 12831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1361 – 13611N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi1365 ↔ 1397 By similarity
Disulfide bondi1610 ↔ 1633 By similarity
Disulfide bondi1785 ↔ 1813 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP97927.
PaxDbiP97927.
PRIDEiP97927.

PTM databases

PhosphoSiteiP97927.

Expressioni

Tissue specificityi

Strongly expressed in peripheral nerves, cardiac muscle, fat, dermis, lung stroma, aortic endothelium, endocardium and endothelium of blood vessels in skin and brain.

Gene expression databases

ArrayExpressiP97927.
BgeeiP97927.
CleanExiMM_LAMA4.
GenevestigatoriP97927.

Interactioni

Subunit structurei

Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-4 is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423).

Protein-protein interaction databases

IntActiP97927. 1 interaction.
MINTiMINT-4100077.

Structurei

3D structure databases

ProteinModelPortaliP97927.
SMRiP97927. Positions 9-300, 829-1373, 1450-1815.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 13150Laminin EGF-like 1
Add
BLAST
Domaini132 – 18655Laminin EGF-like 2
Add
BLAST
Domaini187 – 24054Laminin EGF-like 3
Add
BLAST
Domaini241 – 25515Laminin EGF-like 4; truncated
Add
BLAST
Domaini826 – 1030205Laminin G-like 1
Add
BLAST
Domaini1042 – 1222181Laminin G-like 2
Add
BLAST
Domaini1229 – 1397169Laminin G-like 3
Add
BLAST
Domaini1462 – 1633172Laminin G-like 4
Add
BLAST
Domaini1640 – 1813174Laminin G-like 5
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni256 – 825570Domain II and I
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili431 – 52393 Reviewed prediction
Add
BLAST
Coiled coili556 – 60449 Reviewed prediction
Add
BLAST
Coiled coili655 – 71763 Reviewed prediction
Add
BLAST
Coiled coili770 – 79930 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi717 – 7193Cell attachment site Reviewed prediction

Domaini

The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG247347.
GeneTreeiENSGT00750000117549.
HOGENOMiHOG000113278.
HOVERGENiHBG052299.
InParanoidiQ14BF2.
KOiK06241.
OMAiPKPVDHR.
OrthoDBiEOG7VX8V2.
PhylomeDBiP97927.
TreeFamiTF335359.

Family and domain databases

Gene3Di2.60.120.200. 5 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR002049. EGF_laminin.
IPR001791. Laminin_G.
IPR009254. Laminin_I.
IPR010307. Laminin_II.
[Graphical view]
PfamiPF00053. Laminin_EGF. 3 hits.
PF02210. Laminin_G_2. 5 hits.
PF06008. Laminin_I. 1 hit.
PF06009. Laminin_II. 1 hit.
[Graphical view]
SMARTiSM00180. EGF_Lam. 3 hits.
SM00282. LamG. 5 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 6 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS50025. LAM_G_DOMAIN. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97927-1 [UniParc]FASTAAdd to Basket

« Hide

MGWSTAWCSV LALWLLWCAV CSNAASGDGN AFPFDIEGSA VVGRQDPSET     50
SDSGVTLGRL PPAAERCDAG FFRTLSGECA PCDCNGNSHE CLDGSGFCLH 100
CQRNTTGEHC EKCLDGYIGD SIRGTPRFCQ PCPCPLPHLA NFAESCYRKN 150
GAVRCICKEN YVGPNCERCA PGYYGNPLLI GSTCKKCDCS GNSDPNLIFE 200
DCDEITGQCR NCLRNTTGFK CERCAPGYYG DARTAKNCAV CNCGGGPCDS 250
VTGECLEEGF EVPTGCDKCV WDLTDDLRLA ALSIEESKSG LLSVSSGAAA 300
HRHVTDMNST IHLLRTRLSE RENQYTLRKI QINNSENTLR SLLPDVEGLH 350
EKGSQASRKG MLVEKESMDT IDQATHLVEQ AHNMRDKIQE INSKMLYYGE 400
NQELGPEEIA EKLVLAQKML EEIRSRQPFL THRELVDEEA DEAQELLSQA 450
ENWQRLHNDT RSLFPVVLEQ LDDYNAKLSD LQESINQALD HVRDAEDMNR 500
AITFKQRDHE KQHERVKEQM EVVGASLSMS ADSLTIPQLT LEELDEIIKN 550
ASGIYAEIDG AKNELQGKLS NLSNLSHDLV QEATDHAYNL QQEADELSRN 600
LHSSDMNGLV QKALDASNVY ENIANYVSEA NETAELALNI TDRIYDAVSG 650
IDTQIIYHKD ESDNLLNQAR ELQAKADSSN DEAVADTSRR VGGALWRKGA 700
LRDRLNDAVK QLQAAERGDA HQRLGQSKLF IEEANKTTAA VQQVTTPMAN 750
NLSNWSQNLQ TFDSSAYNTA VDSARDAVRN LTEVVPQLLD QLRTVEQKRP 800
ASNISASIQR IRELIAQTRS VASKIQVSMM FDGQSAVEVH PKVSVDDLKA 850
FTSISLYMKP PPKPAEPTGA WVADQFVLYL GSKNAKKEYM GLAIKNDNLV 900
YVYNLGMKDV EILLDSKPVS SWPAYFSIVK IERVGKHGKV FLTVPSLSST 950
AEEKFIKKGE FAGDDSLLDL TPEDTVFYVG GVPANFKLPA SLNLPSYSGC 1000
LELATLNNDV ISLYNFKHIY NMDPSKSVPC ARDKLAFTQS RAASYFFDGS 1050
SYAVVRDITR RGKFGQVTRF DIEIRTPADN GLVLLMVNGS MFFSLEMRNG 1100
YLHVFYDFGF SNGPVHLEDT LKKAQINDAK YHEISIIYHN DKKMILVVDR 1150
RHVKSTDNEK KKIPFTDIYI GGAPQEVLQS RTLRAHLPLD INFRGCMKGF 1200
QFQKKDFNLL EQTETLGVGY GCPEDSLISR RAYFNGQSFI ASIQKISFFD 1250
GFEGGFNFRT LQPNGLLFYY TSGSDVFSIS LDNGTVVMDV KGIKVMSTDK 1300
QYHDGLPHFV VTSISDTRYE LVVDKSRLRG KNPTKGKAEQ TQTTEKKFYF 1350
GGSPISPQYA NFTGCISNAY FTRLDRDVEV EDFQRYSEKV HTSLYECPIE 1400
SSPLFLLHKK GKNSSKPKTN KQGEKSKDAP SWDPIGLKFL EQKAPRDSHC 1450
HLSSSPRAIE HAYQYGGTAN SRQEFEHEQG DFGEKSQFAI RLKTRSSHGM 1500
IFYVSDQEEN DFMTLFLAHG RLVFMFNVGH KKLKIRSQEK YNDGLWHDVI 1550
FIREKSSGRL VIDGLRVLEE RLPPSGAAWK IKGPIYLGGV APGRAVKNVQ 1600
ITSVYSFSGC LGNLQLNGAS ITSASQTFSV TPCFEGPMET GTYFSTEGGY 1650
VVLDESFNIG LKFEIAFEVR PRSSSGTLVH GHSVNGEYLN VHMRNGQVIV 1700
KVNNGVRDFS TSVTPKQNLC DGRWHRITVI RDSNVVQLDV DSEVNHVVGP 1750
LNPKPVDHRE PVFVGGVPES LLTPRLAPSK PFTGCIRHFV IDSRPVSFSK 1800
AALVSGAVSI NSCPTA 1816
Length:1,816
Mass (Da):201,819
Last modified:December 13, 2001 - v2
Checksum:iB49C45F3A45999D8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81C → S in AAC52982. 1 Publication
Sequence conflicti18 – 181C → Y1 Publication
Sequence conflicti248 – 2481C → R in AAC24725. 1 Publication
Sequence conflicti297 – 2971G → A in AAC24725. 1 Publication
Sequence conflicti431 – 4333THR → HPS in AAC52982. 1 Publication
Sequence conflicti679 – 6791S → C in AAC24725. 1 Publication
Sequence conflicti703 – 7031D → G in AAC52982. 1 Publication
Sequence conflicti706 – 7061N → H in AAC52982. 1 Publication
Sequence conflicti728 – 7281K → R1 Publication
Sequence conflicti730 – 7301F → I1 Publication
Sequence conflicti779 – 7791R → G AA sequence 1 Publication
Sequence conflicti810 – 8101R → S in AAC24725. 1 Publication
Sequence conflicti865 – 8673AEP → QT in AAC52982. 1 Publication
Sequence conflicti936 – 9361K → E in AAC24725. 1 Publication
Sequence conflicti970 – 9701L → V in AAC24725. 1 Publication
Sequence conflicti1132 – 11321H → R in AAC52982. 1 Publication
Sequence conflicti1200 – 12001F → I in AAC52982. 1 Publication
Sequence conflicti1382 – 13821D → A in AAC52982. 1 Publication
Sequence conflicti1413 – 14142NS → EF in CAA70970. 1 Publication
Sequence conflicti1489 – 14891A → S in AAC52982. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U58950 mRNA. Translation: AAB41840.1.
Y09827 mRNA. Translation: CAA70970.1.
U59865 mRNA. Translation: AAC24725.1.
BC115942 mRNA. Translation: AAI15943.1.
U88352 mRNA. Translation: AAC53178.1.
U69176 mRNA. Translation: AAC52982.1.
CCDSiCCDS35882.1.
RefSeqiNP_034811.2. NM_010681.4.
UniGeneiMm.258065.

Genome annotation databases

EnsembliENSMUST00000019992; ENSMUSP00000019992; ENSMUSG00000019846.
GeneIDi16775.
KEGGimmu:16775.
UCSCiuc007evq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U58950 mRNA. Translation: AAB41840.1 .
Y09827 mRNA. Translation: CAA70970.1 .
U59865 mRNA. Translation: AAC24725.1 .
BC115942 mRNA. Translation: AAI15943.1 .
U88352 mRNA. Translation: AAC53178.1 .
U69176 mRNA. Translation: AAC52982.1 .
CCDSi CCDS35882.1.
RefSeqi NP_034811.2. NM_010681.4.
UniGenei Mm.258065.

3D structure databases

ProteinModelPortali P97927.
SMRi P97927. Positions 9-300, 829-1373, 1450-1815.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97927. 1 interaction.
MINTi MINT-4100077.

PTM databases

PhosphoSitei P97927.

Proteomic databases

MaxQBi P97927.
PaxDbi P97927.
PRIDEi P97927.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000019992 ; ENSMUSP00000019992 ; ENSMUSG00000019846 .
GeneIDi 16775.
KEGGi mmu:16775.
UCSCi uc007evq.2. mouse.

Organism-specific databases

CTDi 3910.
MGIi MGI:109321. Lama4.

Phylogenomic databases

eggNOGi NOG247347.
GeneTreei ENSGT00750000117549.
HOGENOMi HOG000113278.
HOVERGENi HBG052299.
InParanoidi Q14BF2.
KOi K06241.
OMAi PKPVDHR.
OrthoDBi EOG7VX8V2.
PhylomeDBi P97927.
TreeFami TF335359.

Enzyme and pathway databases

Reactomei REACT_196606. ECM proteoglycans.
REACT_196607. Non-integrin membrane-ECM interactions.
REACT_202342. Laminin interactions.

Miscellaneous databases

ChiTaRSi LAMA4. mouse.
NextBioi 290616.
PROi P97927.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97927.
Bgeei P97927.
CleanExi MM_LAMA4.
Genevestigatori P97927.

Family and domain databases

Gene3Di 2.60.120.200. 5 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR002049. EGF_laminin.
IPR001791. Laminin_G.
IPR009254. Laminin_I.
IPR010307. Laminin_II.
[Graphical view ]
Pfami PF00053. Laminin_EGF. 3 hits.
PF02210. Laminin_G_2. 5 hits.
PF06008. Laminin_I. 1 hit.
PF06009. Laminin_II. 1 hit.
[Graphical view ]
SMARTi SM00180. EGF_Lam. 3 hits.
SM00282. LamG. 5 hits.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 6 hits.
PROSITEi PS00022. EGF_1. 1 hit.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS50025. LAM_G_DOMAIN. 5 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the mouse laminin alpha 4 cDNA. Expression in a subset of endothelium."
    Frieser M., Noeckel H., Pausch F., Roeder C., Hahn A., Deutzmann R., Sorokin L.M.
    Eur. J. Biochem. 246:727-735(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 462-469; 478-483; 776-782 AND 940-945.
    Strain: BALB/c.
    Tissue: Endothelial cell.
  2. "The complete cDNA coding sequence and tissue-specific expression of the mouse laminin alpha 4 chain."
    Liu J., Mayne R.
    Matrix Biol. 15:433-437(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  3. "Primary structure, developmental expression, and immunolocalization of the murine laminin alpha4 chain."
    Iivanainen A., Kortesmaa J., Sahlberg C., Morita T., Bergmann U., Thesleff I., Tryggvason K.
    J. Biol. Chem. 272:27862-27868(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The laminin alpha chains: expression, developmental transitions, and chromosomal locations of alpha1-5, identification of heterotrimeric laminins 8-11, and cloning of a novel alpha3 isoform."
    Miner J.H., Patton B.L., Lentz S.I., Gilbert D.J., Snider W.D., Jenkins N.A., Copeland N.G., Sanes J.R.
    J. Cell Biol. 137:685-701(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 836-1106.
    Strain: ICR.
    Tissue: Placenta.
  6. "Distribution of the ten known laminin chains in the pathways and targets of developing sensory axons."
    Lentz S.I., Miner J.H., Sanes J.R., Snider W.D.
    J. Comp. Neurol. 378:547-561(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1467-1691.
    Tissue: Placenta.

Entry informationi

Entry nameiLAMA4_MOUSE
AccessioniPrimary (citable) accession number: P97927
Secondary accession number(s): O88785, P70409, Q14BF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: December 13, 2001
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi