Reviewed, UniProtKB/Swiss-Prot P97924 (KALRN_RAT)

Names and origin

Protein names

Recommended name:
    Kalirin
    EC=2.7.11.1
Alternative name(s):
    Huntingtin-associated protein-interacting protein
    Protein Duo
    Serine/threonine kinase with Dbl- and pleckstrin homology domain
    PAM COOH-terminal interactor protein 10
      Short name=P-CIP10

Gene names

Name:	Kalrn
Synonyms:	Duo, Hapip

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	2959 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. Isoforms 1 and 7 are necessary for neuronal development and axonal outgrowth. Isoform 6 is required for dendritic spine formation. Ref.3 Ref.5 Ref.7
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Subunit structure	Interacts with the C-terminal of peptidylglycine alpha-amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1). Ref.2 Ref.8
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Note: Isoform 6 is largely associated with synaptosomal membranes and to punctate structures in cortical neurons. Isoforms 1 and 7 are expressed in neuronal cell bodies, isoform 7 is also found in neuronal processes. Ref.3 Ref.4
Tissue specificity	Highly expressed in the brain and nervous system. Isoform 6 is highly enriched in the postsynaptic density fraction of the cerebral cortex. Ref.3 Ref.4
Developmental stage	Isoforms 1 and 7 are prevelant 2 dpp, isoform 6 is not detectable until 2 weeks after birth. Ref.7
Domain	The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity. Ref.7 Ref.8
Post-translational modification	Autophosphorylated By similarity.
Miscellaneous	Called DUO because the encoded protein is closely related to but shorter than TRIO.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 CRAL-TRIO domain. Contains 2 DH (DBL-homology) domains. Contains 1 fibronectin type-III domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 2 PH domains. Contains 1 protein kinase domain. Contains 2 SH3 domains. Contains 5 spectrin repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative initiation Alternative splicing
Domain	Immunoglobulin domain Repeat SH3 domain
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Guanine-nucleotide releasing factor Kinase Serine/threonine-protein kinase Transferase
PTM	Disulfide bond Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	axonogenesis Inferred from direct assay. Source: RGD intracellular signaling cascade Inferred from direct assay. Source: HGNC protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of dendrite development Inferred from direct assay. Source: RGD vesicle-mediated transport Ref.6 Inferred from direct assay. Source: RGD
Cellular component	cell soma Ref.4 Inferred from direct assay. Source: RGD cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell neuron projection Ref.4 Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Ref.4 Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.1 Ref.6 Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pam	P97467	1	EBI-1397166,EBI-1397216	From a different organism.

Alternative products

This entry describes 8 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform 1 (identifier: P97924-1)

Also known as: Kalirin-12A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by alternative splicing.

Isoform 2 (identifier: P97924-2)

Also known as: Kalirin-8B; P-CIP10B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MVLS → MNPPEGASEEGGAADSDVDAFFRT
     1860-1899: TLEGGSYRGS...LEEEQKAKAL → VSGHAGGSRV...HPDFVYSNCI
     1900-2959: Missing.

Note: Produced by alternative splicing.

Isoform 3 (identifier: P97924-3)

Also known as: Kalirin-8A; P-CIP10A;

The sequence of this isoform differs from the canonical sequence as follows:
     1860-1899: TLEGGSYRGS...LEEEQKAKAL → VSGHAGGSRV...HPDFVYSNCI
     1900-2959: Missing.

Note: Produced by alternative splicing.

Isoform 4 (identifier: P97924-4)

The sequence of this isoform differs from the canonical sequence as follows:
     923-923: E → ESLFHATSLQ

Note: Produced by alternative splicing.

Isoform 5 (identifier: P97924-5)

Also known as: Delta Kalirin-7;

The sequence of this isoform differs from the canonical sequence as follows:
     1-623: Missing.
     1617-1636: LSGGCELTVVLQDFSAAHSS → DGNLVPRWHLGPGDPFSTYV
     1637-2959: Missing.

Note: Produced by alternative initiation at Met-624 of isoform 6.

Isoform 6 (identifier: P97924-6)

Also known as: Kalirin-7; HAPIP;

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MVLS → MTDRFWDQWYLWYLRLLRLLDR
     1617-1636: LSGGCELTVVLQDFSAAHSS → DGNLVPRWHLGPGDPFSTYV
     1637-2959: Missing.

Note: Produced by alternative splicing.

Isoform 7 (identifier: P97924-7)

Also known as: Kalirin-9A;

The sequence of this isoform differs from the canonical sequence as follows:
     2372-2376: KSCSW → TLLKP
     2377-2959: Missing.

Note: Produced by alternative splicing.

Isoform 8 (identifier: P97924-8)

The sequence of this isoform differs from the canonical sequence as follows:
     705-718: DSAVSNNKTPHSSS → SAWAVIPVGNASG
     719-2959: Missing.

Note: Produced by alternative splicing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 2959

2959

Kalirin

PRO_0000080956

Regions

Domain

17 – 162

146

CRAL-TRIO

Repeat

170 – 290

121

Spectrin 1

Repeat

292 – 398

107

Spectrin 2

Repeat

518 – 624

107

Spectrin 3

Repeat

872 – 977

106

Spectrin 4

Repeat

1103 – 1209

107

Spectrin 5

Domain

1254 – 1429

176

DH 1

Domain

1441 – 1553

113

PH 1

Domain

1619 – 1684

66

SH3 1

Domain

1901 – 2076

176

DH 2

Domain

2088 – 2198

111

PH 2

Domain

2293 – 2358

66

SH3 2

Domain

2444 – 2537

94

Ig-like C2-type

Domain

2542 – 2633

92

Fibronectin type-III

Domain

2657 – 2911

255

Protein kinase

Nucleotide binding

2663 – 2671

9

ATP By similarity

Compositional bias

664 – 669

6

Poly-Gln

Sites

Active site

2776

1

Proton acceptor By similarity

Binding site

2686

1

ATP By similarity

Amino acid modifications

Modified residue

1723

1

Phosphoserine By similarity

Modified residue

1726

1

Phosphoserine By similarity

Modified residue

1729

1

Phosphoserine By similarity

Modified residue

1746

1

Phosphoserine By similarity

Modified residue

1772

1

Phosphoserine By similarity

Modified residue

1790

1

Phosphoserine By similarity

Modified residue

2209

1

Phosphoserine By similarity

Modified residue

2234

1

Phosphoserine By similarity

Disulfide bond

2465 ↔ 2521

By similarity

Natural variations

Alternative sequence

1 – 623

623

Missing in isoform 5.

VSP_028893

Alternative sequence

1 – 4

4

MVLS → MNPPEGASEEGGAADSDVDA FFRT in isoform 2.

VSP_028891

Alternative sequence

1 – 4

4

MVLS → MTDRFWDQWYLWYLRLLRLL DR in isoform 6.

VSP_028892

Alternative sequence

705 – 718

14

DSAVS…PHSSS → SAWAVIPVGNASG in isoform 8.

VSP_028894

Alternative sequence

719 – 2959

2241

Missing in isoform 8.

VSP_028895

Alternative sequence

923

1

E → ESLFHATSLQ in isoform 4.

VSP_028896

Alternative sequence

1617 – 1636

20

LSGGC…AAHSS → DGNLVPRWHLGPGDPFSTYV in isoform 5 and isoform 6.

VSP_028897

Alternative sequence

1637 – 2959

1323

Missing in isoform 5 and isoform 6.

VSP_028898

Alternative sequence

1860 – 1899

40

TLEGG…KAKAL → VSGHAGGSRVLPLTSMWLPG PQLGPQISYLHPDFVYSNCI in isoform 2 and isoform 3.

VSP_028899

Alternative sequence

1900 – 2959

1060

Missing in isoform 2 and isoform 3.

VSP_028900

Alternative sequence

2372 – 2376

5

KSCSW → TLLKP in isoform 7.

VSP_028901

Alternative sequence

2377 – 2959

583

Missing in isoform 7.

VSP_028902

Experimental info

Sequence conflict

46

1

T → S in AAT39517. Ref.5

Sequence conflict

62

1

N → Y in AAT39517. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

2959

Helix Strand Turn

Details...

Beta strand

1622 – 1625

4

Beta strand

1645 – 1650

6

Beta strand

1658 – 1668

11

Beta strand

1670 – 1675

6

Helix

1676 – 1678

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Kalirin-12A) [UniParc]. Last modified October 23, 2007. Version 3. Checksum: 1A3C6770D1CBCC1F	FASTA	2,959	336,587
	10 20 30 40 50 60 MVLSGSFRND GLKASDVLPI LKEKVAFVSG GRDKRGGPIL TFPARTNHDR IRQEDLRKLV 70 80 90 100 110 120 TNLASVPSED VCKRGFTVII DMRGSKWDLI KPLLKTLQEA FPAEIHVALI IKPDNFWQKQ 130 140 150 160 170 180 KTNFGSSKFI FETSMVSVEG LTKLVDPSQL TEEFDGSLDY NHEEWIELRL SLEEFFNSAV 190 200 210 220 230 240 HLLSRLEDLQ EMLARKEFPV DVEGSRRLID EHTQLKKKVL KAPVEELDRE GQRLLQCIRC 250 260 270 280 290 300 SDGFSGRNCI PGSADFQSLV PKITSLLDKL HSTRQHLHQM WHVRKLKLDQ CFQLRLFEQD 310 320 330 340 350 360 AEKMFDWISH NKELFLQSHT EIGVSYQHAL DLQTQHNHFA MNSMNAYVNI NRIMSVASRL 370 380 390 400 410 420 SEAGHYASQQ IKQISTQLDQ EWKSFAAALD ERSTILAMSA VFHQKAEQFL SGVDAWCKMC 430 440 450 460 470 480 SEGGLPSEMQ DLELAIHHHQ SLYEQVTQAY TEVSQDGKAL LDVLQRPLSP GNSESLTATA 490 500 510 520 530 540 NYSKAVHQVL DVVHEVLHHQ RRLESIWQHR KVRLHQRLQL CVFQQDVQQV LDWIENHGEA 550 560 570 580 590 600 FLSKHTGVGK SLHRARALQK RHDDFEEVAQ NTYTNADKLL EAAEQLAQTG ECDPEEIYKA 610 620 630 640 650 660 ARHLEVRIQD FVRRVEQRKL LLDMSVSFHT HTKELWTWME DLQKEVLEDV CADSVDAVQE 670 680 690 700 710 720 LIKQFQQQQT ATLDATLNVI KEGEDLIQQL RSAPPSLGEP TEARDSAVSN NKTPHSSSIS 730 740 750 760 770 780 HIESVLQQLD DAQVQMEELF HERKIKLDIF LQLRIFEQYT IEVTAELDAW NEDLLRQMND 790 800 810 820 830 840 FNTEDLTLAE QRLQRHTERK LAMNNMTFEV IQQGQDLHQY IMEVQASGIE LICEKDVDLA 850 860 870 880 890 900 AQVQELLEFL HEKQHELELN AEQTHKRLEQ CLQLRHLQAE VKQVLGWIRN GESMLNASLV 910 920 930 940 950 960 NASSLSEAEQ LQREHEQFQL AIEKTHQSAL QVQQKAEALL QAGHYDADAI RECAEKVALH 970 980 990 1000 1010 1020 WQQLMLKMED RLKLVNASVA FYKTSEQVCS VLESLEQEYR RDEDWCGGRD KLGPAAEMDH 1030 1040 1050 1060 1070 1080 VIPLLSKHLE QKEAFLKACT LARRNAEVFL KYIHRNNVSM PSVASHTRGP EQQVKAILSE 1090 1100 1110 1120 1130 1140 LLQRENRVLH FWTLKKRRLD QCQQYVVFER SAKQALDWIQ ETGEYYLSTH TSTGETTEET 1150 1160 1170 1180 1190 1200 QELLKEYGEF RVPAKQTKEK VKLLIQLADS FVEKGHIHAT EIRKWVTTVD KHYRDFSLRM 1210 1220 1230 1240 1250 1260 GKYRYTLEKA LGVNTEDNKD LELDIIPASL SDREVKLRDA NHEVNEEKRK SARKKEFIMA 1270 1280 1290 1300 1310 1320 ELLQTEKAYV RDLHECLETY LWEMTSGVEE IPPGILNKEH IIFGNIQEIY DFHNNIFLKE 1330 1340 1350 1360 1370 1380 LEKYEQLPED VGHCFVTWAD KFQMYVTYCK NKPDSNQLIL EHAGTFFDEI QQRHGLANSI 1390 1400 1410 1420 1430 1440 SSYLIKPVQR VTKYQLLLKE LLTCCEEGKG ELKDGLEVML SVPKKANDAM HVSMLEGFDE 1450 1460 1470 1480 1490 1500 NLDVQGELIL QDAFQVWDPK SLIRKGRERH LFLFEISLVF SKEIKDSSGH TKYVYKNKLL 1510 1520 1530 1540 1550 1560 TSELGVTEHV EGDPCKFALW SGRTPSSDNK TVLKASNIET KQEWIKNIRE VIQERIIHLK 1570 1580 1590 1600 1610 1620 GALKEPIQLP KTPAKLRNNS KRDGVEDGDS QGDGSSQPDT ISIASRTSQN TVESDKLSGG 1630 1640 1650 1660 1670 1680 CELTVVLQDF SAAHSSELSI QVGQTVELLE RPSERPGWCL VRTTERSPPQ EGLVPSSTLC 1690 1700 1710 1720 1730 1740 ISHSRSSVEM DCFFPLVKDS YSHSSGENGG KSESVANLQS QPSLNSIHSS PGPKRSTNTL 1750 1760 1770 1780 1790 1800 KKWLTSPVRR LNSGKADGNI KKQKKVRDGR KSFDLGSPKP GDETTPQGDS ADEKSKKGWG 1810 1820 1830 1840 1850 1860 EDEPDEESHT PLPPPMKIFD NDPTQDEMSS LLAARQAPTD VPTAADLVSA IEKLVKSKLT 1870 1880 1890 1900 1910 1920 LEGGSYRGSL KDPTVCLNEG MAPPTPPRNL EEEQKAKALR GRMFVLNELV QTEKDYVKDL 1930 1940 1950 1960 1970 1980 GIVVEGFMKR IEEKGVPEDM RGKEKIVFGN IHQIYDWHKD FFLAELEKCI QEQDRLAQLF 1990 2000 2010 2020 2030 2040 IKHERKLHIY VWYCQNKPRS EYIVAEYDAY FEEVKQEINQ RLTLSDFLIK PIQRITKYQL 2050 2060 2070 2080 2090 2100 LLKDFLRYSE KAGLECSDIE KAVELMCLVP KRCNDMMNLG RLQGFEGTLT AQGKLLQQDT 2110 2120 2130 2140 2150 2160 FYVIELDAGM QSRTKERRVF LFEQIVIFSE LLRKGSLTPG YMFKRSIKMN YLVLEEDVDD 2170 2180 2190 2200 2210 2220 DPCKFALMNR ETSERVILQA ANSDIQQAWV QDINQVLETQ RDFLNALQSP IEYQRKERST 2230 2240 2250 2260 2270 2280 AVIRSQPPRV PQASPRPYSS VPVGSEKPPK GSSYNPPLPP LKISTSNGSP GFDYHQPGDK 2290 2300 2310 2320 2330 2340 FDASKQNDLG GCNGTSTMAV IKDYYALKEN EICVSQGEVV QVLAVNQQNM CLVYQPASDH 2350 2360 2370 2380 2390 2400 SPAAEGWVPG SILAPLTKAT APAESSDESI KKSCSWHTLR MRKRADVENT GKNEATGPRK 2410 2420 2430 2440 2450 2460 PKDILGNKAS VKETNSSEES ECDDLDPNTS MEILNPNFIQ EVAPEFLVPL VDVTCLLGDT 2470 2480 2490 2500 2510 2520 VLLQCKACGR PKPTITWKGP DQNILDTDNS SATYTISSCD SGESTLKICN LMPQDSGIYT 2530 2540 2550 2560 2570 2580 CIATNDHGTA STSATVKVQG VPAAPNRPIA QERSCTSVIL RWLPPASTGN CTISGYTVEY 2590 2600 2610 2620 2630 2640 REEGSQVWQQ SVASTLDTYL VIEDLSPGCP YQFRVSASNP WGISLPSEPS EFVHLPEYDA 2650 2660 2670 2680 2690 2700 AADGATISWK ENFDSAYTEL NEIGRGRFSI VKKCIHKATR KDVAVKFVSK KMKKKEQAAH 2710 2720 2730 2740 2750 2760 EAALLQHLQH PQYVTLHDTY ESPTSYILIL ELMDDGRLLD YLMNHDELME EKVAFYIRDI 2770 2780 2790 2800 2810 2820 MEALQYLHNC RVAHLDIKPE NLLIDLRIPV PRVKLIDLED AVQISGHFHI HHLLGNPEFA 2830 2840 2850 2860 2870 2880 APEVIQGIPV SLGTDIWSIG VLTYVMLSGV SPFLDESKEE TCINVCRVDF SFPHEYFCGV 2890 2900 2910 2920 2930 2940 SNAARDFINV ILQEDFRRRP TAATCLQHPW LQPHNGSYSK IPLDTSRLAC FIERRKHQND 2950 VRPIPNVKSY IVNRVNQGT
	Isoform 2 (Kalirin-8B) (P-CIP10B). Checksum: E4DBB818E890F453	FASTA	1,919	218,841
	10 20 30 40 50 60 MNPPEGASEE GGAADSDVDA FFRTGSFRND GLKASDVLPI LKEKVAFVSG GRDKRGGPIL 70 80 90 100 110 120 TFPARTNHDR IRQEDLRKLV TNLASVPSED VCKRGFTVII DMRGSKWDLI KPLLKTLQEA 130 140 150 160 170 180 FPAEIHVALI IKPDNFWQKQ KTNFGSSKFI FETSMVSVEG LTKLVDPSQL TEEFDGSLDY 190 200 210 220 230 240 NHEEWIELRL SLEEFFNSAV HLLSRLEDLQ EMLARKEFPV DVEGSRRLID EHTQLKKKVL 250 260 270 280 290 300 KAPVEELDRE GQRLLQCIRC SDGFSGRNCI PGSADFQSLV PKITSLLDKL HSTRQHLHQM 310 320 330 340 350 360 WHVRKLKLDQ CFQLRLFEQD AEKMFDWISH NKELFLQSHT EIGVSYQHAL DLQTQHNHFA 370 380 390 400 410 420 MNSMNAYVNI NRIMSVASRL SEAGHYASQQ IKQISTQLDQ EWKSFAAALD ERSTILAMSA 430 440 450 460 470 480 VFHQKAEQFL SGVDAWCKMC SEGGLPSEMQ DLELAIHHHQ SLYEQVTQAY TEVSQDGKAL 490 500 510 520 530 540 LDVLQRPLSP GNSESLTATA NYSKAVHQVL DVVHEVLHHQ RRLESIWQHR KVRLHQRLQL 550 560 570 580 590 600 CVFQQDVQQV LDWIENHGEA FLSKHTGVGK SLHRARALQK RHDDFEEVAQ NTYTNADKLL 610 620 630 640 650 660 EAAEQLAQTG ECDPEEIYKA ARHLEVRIQD FVRRVEQRKL LLDMSVSFHT HTKELWTWME 670 680 690 700 710 720 DLQKEVLEDV CADSVDAVQE LIKQFQQQQT ATLDATLNVI KEGEDLIQQL RSAPPSLGEP 730 740 750 760 770 780 TEARDSAVSN NKTPHSSSIS HIESVLQQLD DAQVQMEELF HERKIKLDIF LQLRIFEQYT 790 800 810 820 830 840 IEVTAELDAW NEDLLRQMND FNTEDLTLAE QRLQRHTERK LAMNNMTFEV IQQGQDLHQY 850 860 870 880 890 900 IMEVQASGIE LICEKDVDLA AQVQELLEFL HEKQHELELN AEQTHKRLEQ CLQLRHLQAE 910 920 930 940 950 960 VKQVLGWIRN GESMLNASLV NASSLSEAEQ LQREHEQFQL AIEKTHQSAL QVQQKAEALL 970 980 990 1000 1010 1020 QAGHYDADAI RECAEKVALH WQQLMLKMED RLKLVNASVA FYKTSEQVCS VLESLEQEYR 1030 1040 1050 1060 1070 1080 RDEDWCGGRD KLGPAAEMDH VIPLLSKHLE QKEAFLKACT LARRNAEVFL KYIHRNNVSM 1090 1100 1110 1120 1130 1140 PSVASHTRGP EQQVKAILSE LLQRENRVLH FWTLKKRRLD QCQQYVVFER SAKQALDWIQ 1150 1160 1170 1180 1190 1200 ETGEYYLSTH TSTGETTEET QELLKEYGEF RVPAKQTKEK VKLLIQLADS FVEKGHIHAT 1210 1220 1230 1240 1250 1260 EIRKWVTTVD KHYRDFSLRM GKYRYTLEKA LGVNTEDNKD LELDIIPASL SDREVKLRDA 1270 1280 1290 1300 1310 1320 NHEVNEEKRK SARKKEFIMA ELLQTEKAYV RDLHECLETY LWEMTSGVEE IPPGILNKEH 1330 1340 1350 1360 1370 1380 IIFGNIQEIY DFHNNIFLKE LEKYEQLPED VGHCFVTWAD KFQMYVTYCK NKPDSNQLIL 1390 1400 1410 1420 1430 1440 EHAGTFFDEI QQRHGLANSI SSYLIKPVQR VTKYQLLLKE LLTCCEEGKG ELKDGLEVML 1450 1460 1470 1480 1490 1500 SVPKKANDAM HVSMLEGFDE NLDVQGELIL QDAFQVWDPK SLIRKGRERH LFLFEISLVF 1510 1520 1530 1540 1550 1560 SKEIKDSSGH TKYVYKNKLL TSELGVTEHV EGDPCKFALW SGRTPSSDNK TVLKASNIET 1570 1580 1590 1600 1610 1620 KQEWIKNIRE VIQERIIHLK GALKEPIQLP KTPAKLRNNS KRDGVEDGDS QGDGSSQPDT 1630 1640 1650 1660 1670 1680 ISIASRTSQN TVESDKLSGG CELTVVLQDF SAAHSSELSI QVGQTVELLE RPSERPGWCL 1690 1700 1710 1720 1730 1740 VRTTERSPPQ EGLVPSSTLC ISHSRSSVEM DCFFPLVKDS YSHSSGENGG KSESVANLQS 1750 1760 1770 1780 1790 1800 QPSLNSIHSS PGPKRSTNTL KKWLTSPVRR LNSGKADGNI KKQKKVRDGR KSFDLGSPKP 1810 1820 1830 1840 1850 1860 GDETTPQGDS ADEKSKKGWG EDEPDEESHT PLPPPMKIFD NDPTQDEMSS LLAARQAPTD 1870 1880 1890 1900 1910 VPTAADLVSA IEKLVKSKLV SGHAGGSRVL PLTSMWLPGP QLGPQISYLH PDFVYSNCI
	Isoform 3 (Kalirin-8A) (P-CIP10A). Checksum: E3545E4CB9F59A1E	FASTA	1,899	216,819
	10 20 30 40 50 60 MVLSGSFRND GLKASDVLPI LKEKVAFVSG GRDKRGGPIL TFPARTNHDR IRQEDLRKLV 70 80 90 100 110 120 TNLASVPSED VCKRGFTVII DMRGSKWDLI KPLLKTLQEA FPAEIHVALI IKPDNFWQKQ 130 140 150 160 170 180 KTNFGSSKFI FETSMVSVEG LTKLVDPSQL TEEFDGSLDY NHEEWIELRL SLEEFFNSAV 190 200 210 220 230 240 HLLSRLEDLQ EMLARKEFPV DVEGSRRLID EHTQLKKKVL KAPVEELDRE GQRLLQCIRC 250 260 270 280 290 300 SDGFSGRNCI PGSADFQSLV PKITSLLDKL HSTRQHLHQM WHVRKLKLDQ CFQLRLFEQD 310 320 330 340 350 360 AEKMFDWISH NKELFLQSHT EIGVSYQHAL DLQTQHNHFA MNSMNAYVNI NRIMSVASRL 370 380 390 400 410 420 SEAGHYASQQ IKQISTQLDQ EWKSFAAALD ERSTILAMSA VFHQKAEQFL SGVDAWCKMC 430 440 450 460 470 480 SEGGLPSEMQ DLELAIHHHQ SLYEQVTQAY TEVSQDGKAL LDVLQRPLSP GNSESLTATA 490 500 510 520 530 540 NYSKAVHQVL DVVHEVLHHQ RRLESIWQHR KVRLHQRLQL CVFQQDVQQV LDWIENHGEA 550 560 570 580 590 600 FLSKHTGVGK SLHRARALQK RHDDFEEVAQ NTYTNADKLL EAAEQLAQTG ECDPEEIYKA 610 620 630 640 650 660 ARHLEVRIQD FVRRVEQRKL LLDMSVSFHT HTKELWTWME DLQKEVLEDV CADSVDAVQE 670 680 690 700 710 720 LIKQFQQQQT ATLDATLNVI KEGEDLIQQL RSAPPSLGEP TEARDSAVSN NKTPHSSSIS 730 740 750 760 770 780 HIESVLQQLD DAQVQMEELF HERKIKLDIF LQLRIFEQYT IEVTAELDAW NEDLLRQMND 790 800 810 820 830 840 FNTEDLTLAE QRLQRHTERK LAMNNMTFEV IQQGQDLHQY IMEVQASGIE LICEKDVDLA 850 860 870 880 890 900 AQVQELLEFL HEKQHELELN AEQTHKRLEQ CLQLRHLQAE VKQVLGWIRN GESMLNASLV 910 920 930 940 950 960 NASSLSEAEQ LQREHEQFQL AIEKTHQSAL QVQQKAEALL QAGHYDADAI RECAEKVALH 970 980 990 1000 1010 1020 WQQLMLKMED RLKLVNASVA FYKTSEQVCS VLESLEQEYR RDEDWCGGRD KLGPAAEMDH 1030 1040 1050 1060 1070 1080 VIPLLSKHLE QKEAFLKACT LARRNAEVFL KYIHRNNVSM PSVASHTRGP EQQVKAILSE 1090 1100 1110 1120 1130 1140 LLQRENRVLH FWTLKKRRLD QCQQYVVFER SAKQALDWIQ ETGEYYLSTH TSTGETTEET 1150 1160 1170 1180 1190 1200 QELLKEYGEF RVPAKQTKEK VKLLIQLADS FVEKGHIHAT EIRKWVTTVD KHYRDFSLRM 1210 1220 1230 1240 1250 1260 GKYRYTLEKA LGVNTEDNKD LELDIIPASL SDREVKLRDA NHEVNEEKRK SARKKEFIMA 1270 1280 1290 1300 1310 1320 ELLQTEKAYV RDLHECLETY LWEMTSGVEE IPPGILNKEH IIFGNIQEIY DFHNNIFLKE 1330 1340 1350 1360 1370 1380 LEKYEQLPED VGHCFVTWAD KFQMYVTYCK NKPDSNQLIL EHAGTFFDEI QQRHGLANSI 1390 1400 1410 1420 1430 1440 SSYLIKPVQR VTKYQLLLKE LLTCCEEGKG ELKDGLEVML SVPKKANDAM HVSMLEGFDE 1450 1460 1470 1480 1490 1500 NLDVQGELIL QDAFQVWDPK SLIRKGRERH LFLFEISLVF SKEIKDSSGH TKYVYKNKLL 1510 1520 1530 1540 1550 1560 TSELGVTEHV EGDPCKFALW SGRTPSSDNK TVLKASNIET KQEWIKNIRE VIQERIIHLK 1570 1580 1590 1600 1610 1620 GALKEPIQLP KTPAKLRNNS KRDGVEDGDS QGDGSSQPDT ISIASRTSQN TVESDKLSGG 1630 1640 1650 1660 1670 1680 CELTVVLQDF SAAHSSELSI QVGQTVELLE RPSERPGWCL VRTTERSPPQ EGLVPSSTLC 1690 1700 1710 1720 1730 1740 ISHSRSSVEM DCFFPLVKDS YSHSSGENGG KSESVANLQS QPSLNSIHSS PGPKRSTNTL 1750 1760 1770 1780 1790 1800 KKWLTSPVRR LNSGKADGNI KKQKKVRDGR KSFDLGSPKP GDETTPQGDS ADEKSKKGWG 1810 1820 1830 1840 1850 1860 EDEPDEESHT PLPPPMKIFD NDPTQDEMSS LLAARQAPTD VPTAADLVSA IEKLVKSKLV 1870 1880 1890 SGHAGGSRVL PLTSMWLPGP QLGPQISYLH PDFVYSNCI
	Isoform 4. Checksum: 6B8B3C0E9FD8CDDC	FASTA	2,968	337,572
	10 20 30 40 50 60 MVLSGSFRND GLKASDVLPI LKEKVAFVSG GRDKRGGPIL TFPARTNHDR IRQEDLRKLV 70 80 90 100 110 120 TNLASVPSED VCKRGFTVII DMRGSKWDLI KPLLKTLQEA FPAEIHVALI IKPDNFWQKQ 130 140 150 160 170 180 KTNFGSSKFI FETSMVSVEG LTKLVDPSQL TEEFDGSLDY NHEEWIELRL SLEEFFNSAV 190 200 210 220 230 240 HLLSRLEDLQ EMLARKEFPV DVEGSRRLID EHTQLKKKVL KAPVEELDRE GQRLLQCIRC 250 260 270 280 290 300 SDGFSGRNCI PGSADFQSLV PKITSLLDKL HSTRQHLHQM WHVRKLKLDQ CFQLRLFEQD 310 320 330 340 350 360 AEKMFDWISH NKELFLQSHT EIGVSYQHAL DLQTQHNHFA MNSMNAYVNI NRIMSVASRL 370 380 390 400 410 420 SEAGHYASQQ IKQISTQLDQ EWKSFAAALD ERSTILAMSA VFHQKAEQFL SGVDAWCKMC 430 440 450 460 470 480 SEGGLPSEMQ DLELAIHHHQ SLYEQVTQAY TEVSQDGKAL LDVLQRPLSP GNSESLTATA 490 500 510 520 530 540 NYSKAVHQVL DVVHEVLHHQ RRLESIWQHR KVRLHQRLQL CVFQQDVQQV LDWIENHGEA 550 560 570 580 590 600 FLSKHTGVGK SLHRARALQK RHDDFEEVAQ NTYTNADKLL EAAEQLAQTG ECDPEEIYKA 610 620 630 640 650 660 ARHLEVRIQD FVRRVEQRKL LLDMSVSFHT HTKELWTWME DLQKEVLEDV CADSVDAVQE 670 680 690 700 710 720 LIKQFQQQQT ATLDATLNVI KEGEDLIQQL RSAPPSLGEP TEARDSAVSN NKTPHSSSIS 730 740 750 760 770 780 HIESVLQQLD DAQVQMEELF HERKIKLDIF LQLRIFEQYT IEVTAELDAW NEDLLRQMND 790 800 810 820 830 840 FNTEDLTLAE QRLQRHTERK LAMNNMTFEV IQQGQDLHQY IMEVQASGIE LICEKDVDLA 850 860 870 880 890 900 AQVQELLEFL HEKQHELELN AEQTHKRLEQ CLQLRHLQAE VKQVLGWIRN GESMLNASLV 910 920 930 940 950 960 NASSLSEAEQ LQREHEQFQL AIESLFHATS LQKTHQSALQ VQQKAEALLQ AGHYDADAIR 970 980 990 1000 1010 1020 ECAEKVALHW QQLMLKMEDR LKLVNASVAF YKTSEQVCSV LESLEQEYRR DEDWCGGRDK 1030 1040 1050 1060 1070 1080 LGPAAEMDHV IPLLSKHLEQ KEAFLKACTL ARRNAEVFLK YIHRNNVSMP SVASHTRGPE 1090 1100 1110 1120 1130 1140 QQVKAILSEL LQRENRVLHF WTLKKRRLDQ CQQYVVFERS AKQALDWIQE TGEYYLSTHT 1150 1160 1170 1180 1190 1200 STGETTEETQ ELLKEYGEFR VPAKQTKEKV KLLIQLADSF VEKGHIHATE IRKWVTTVDK 1210 1220 1230 1240 1250 1260 HYRDFSLRMG KYRYTLEKAL GVNTEDNKDL ELDIIPASLS DREVKLRDAN HEVNEEKRKS 1270 1280 1290 1300 1310 1320 ARKKEFIMAE LLQTEKAYVR DLHECLETYL WEMTSGVEEI PPGILNKEHI IFGNIQEIYD 1330 1340 1350 1360 1370 1380 FHNNIFLKEL EKYEQLPEDV GHCFVTWADK FQMYVTYCKN KPDSNQLILE HAGTFFDEIQ 1390 1400 1410 1420 1430 1440 QRHGLANSIS SYLIKPVQRV TKYQLLLKEL LTCCEEGKGE LKDGLEVMLS VPKKANDAMH 1450 1460 1470 1480 1490 1500 VSMLEGFDEN LDVQGELILQ DAFQVWDPKS LIRKGRERHL FLFEISLVFS KEIKDSSGHT 1510 1520 1530 1540 1550 1560 KYVYKNKLLT SELGVTEHVE GDPCKFALWS GRTPSSDNKT VLKASNIETK QEWIKNIREV 1570 1580 1590 1600 1610 1620 IQERIIHLKG ALKEPIQLPK TPAKLRNNSK RDGVEDGDSQ GDGSSQPDTI SIASRTSQNT 1630 1640 1650 1660 1670 1680 VESDKLSGGC ELTVVLQDFS AAHSSELSIQ VGQTVELLER PSERPGWCLV RTTERSPPQE 1690 1700 1710 1720 1730 1740 GLVPSSTLCI SHSRSSVEMD CFFPLVKDSY SHSSGENGGK SESVANLQSQ PSLNSIHSSP 1750 1760 1770 1780 1790 1800 GPKRSTNTLK KWLTSPVRRL NSGKADGNIK KQKKVRDGRK SFDLGSPKPG DETTPQGDSA 1810 1820 1830 1840 1850 1860 DEKSKKGWGE DEPDEESHTP LPPPMKIFDN DPTQDEMSSL LAARQAPTDV PTAADLVSAI 1870 1880 1890 1900 1910 1920 EKLVKSKLTL EGGSYRGSLK DPTVCLNEGM APPTPPRNLE EEQKAKALRG RMFVLNELVQ 1930 1940 1950 1960 1970 1980 TEKDYVKDLG IVVEGFMKRI EEKGVPEDMR GKEKIVFGNI HQIYDWHKDF FLAELEKCIQ 1990 2000 2010 2020 2030 2040 EQDRLAQLFI KHERKLHIYV WYCQNKPRSE YIVAEYDAYF EEVKQEINQR LTLSDFLIKP 2050 2060 2070 2080 2090 2100 IQRITKYQLL LKDFLRYSEK AGLECSDIEK AVELMCLVPK RCNDMMNLGR LQGFEGTLTA 2110 2120 2130 2140 2150 2160 QGKLLQQDTF YVIELDAGMQ SRTKERRVFL FEQIVIFSEL LRKGSLTPGY MFKRSIKMNY 2170 2180 2190 2200 2210 2220 LVLEEDVDDD PCKFALMNRE TSERVILQAA NSDIQQAWVQ DINQVLETQR DFLNALQSPI 2230 2240 2250 2260 2270 2280 EYQRKERSTA VIRSQPPRVP QASPRPYSSV PVGSEKPPKG SSYNPPLPPL KISTSNGSPG 2290 2300 2310 2320 2330 2340 FDYHQPGDKF DASKQNDLGG CNGTSTMAVI KDYYALKENE ICVSQGEVVQ VLAVNQQNMC 2350 2360 2370 2380 2390 2400 LVYQPASDHS PAAEGWVPGS ILAPLTKATA PAESSDESIK KSCSWHTLRM RKRADVENTG 2410 2420 2430 2440 2450 2460 KNEATGPRKP KDILGNKASV KETNSSEESE CDDLDPNTSM EILNPNFIQE VAPEFLVPLV 2470 2480 2490 2500 2510 2520 DVTCLLGDTV LLQCKACGRP KPTITWKGPD QNILDTDNSS ATYTISSCDS GESTLKICNL 2530 2540 2550 2560 2570 2580 MPQDSGIYTC IATNDHGTAS TSATVKVQGV PAAPNRPIAQ ERSCTSVILR WLPPASTGNC 2590 2600 2610 2620 2630 2640 TISGYTVEYR EEGSQVWQQS VASTLDTYLV IEDLSPGCPY QFRVSASNPW GISLPSEPSE 2650 2660 2670 2680 2690 2700 FVHLPEYDAA ADGATISWKE NFDSAYTELN EIGRGRFSIV KKCIHKATRK DVAVKFVSKK 2710 2720 2730 2740 2750 2760 MKKKEQAAHE AALLQHLQHP QYVTLHDTYE SPTSYILILE LMDDGRLLDY LMNHDELMEE 2770 2780 2790 2800 2810 2820 KVAFYIRDIM EALQYLHNCR VAHLDIKPEN LLIDLRIPVP RVKLIDLEDA VQISGHFHIH 2830 2840 2850 2860 2870 2880 HLLGNPEFAA PEVIQGIPVS LGTDIWSIGV LTYVMLSGVS PFLDESKEET CINVCRVDFS 2890 2900 2910 2920 2930 2940 FPHEYFCGVS NAARDFINVI LQEDFRRRPT AATCLQHPWL QPHNGSYSKI PLDTSRLACF 2950 2960 IERRKHQNDV RPIPNVKSYI VNRVNQGT
	Isoform 5 (Delta Kalirin-7). Checksum: 8C24887FBE316B64	FASTA	1,013	116,939
	10 20 30 40 50 60 MSVSFHTHTK ELWTWMEDLQ KEVLEDVCAD SVDAVQELIK QFQQQQTATL DATLNVIKEG 70 80 90 100 110 120 EDLIQQLRSA PPSLGEPTEA RDSAVSNNKT PHSSSISHIE SVLQQLDDAQ VQMEELFHER 130 140 150 160 170 180 KIKLDIFLQL RIFEQYTIEV TAELDAWNED LLRQMNDFNT EDLTLAEQRL QRHTERKLAM 190 200 210 220 230 240 NNMTFEVIQQ GQDLHQYIME VQASGIELIC EKDVDLAAQV QELLEFLHEK QHELELNAEQ 250 260 270 280 290 300 THKRLEQCLQ LRHLQAEVKQ VLGWIRNGES MLNASLVNAS SLSEAEQLQR EHEQFQLAIE 310 320 330 340 350 360 KTHQSALQVQ QKAEALLQAG HYDADAIREC AEKVALHWQQ LMLKMEDRLK LVNASVAFYK 370 380 390 400 410 420 TSEQVCSVLE SLEQEYRRDE DWCGGRDKLG PAAEMDHVIP LLSKHLEQKE AFLKACTLAR 430 440 450 460 470 480 RNAEVFLKYI HRNNVSMPSV ASHTRGPEQQ VKAILSELLQ RENRVLHFWT LKKRRLDQCQ 490 500 510 520 530 540 QYVVFERSAK QALDWIQETG EYYLSTHTST GETTEETQEL LKEYGEFRVP AKQTKEKVKL 550 560 570 580 590 600 LIQLADSFVE KGHIHATEIR KWVTTVDKHY RDFSLRMGKY RYTLEKALGV NTEDNKDLEL 610 620 630 640 650 660 DIIPASLSDR EVKLRDANHE VNEEKRKSAR KKEFIMAELL QTEKAYVRDL HECLETYLWE 670 680 690 700 710 720 MTSGVEEIPP GILNKEHIIF GNIQEIYDFH NNIFLKELEK YEQLPEDVGH CFVTWADKFQ 730 740 750 760 770 780 MYVTYCKNKP DSNQLILEHA GTFFDEIQQR HGLANSISSY LIKPVQRVTK YQLLLKELLT 790 800 810 820 830 840 CCEEGKGELK DGLEVMLSVP KKANDAMHVS MLEGFDENLD VQGELILQDA FQVWDPKSLI 850 860 870 880 890 900 RKGRERHLFL FEISLVFSKE IKDSSGHTKY VYKNKLLTSE LGVTEHVEGD PCKFALWSGR 910 920 930 940 950 960 TPSSDNKTVL KASNIETKQE WIKNIREVIQ ERIIHLKGAL KEPIQLPKTP AKLRNNSKRD 970 980 990 1000 1010 GVEDGDSQGD GSSQPDTISI ASRTSQNTVE SDKDGNLVPR WHLGPGDPFS TYV
	Isoform 6 (Kalirin-7) (HAPIP). Checksum: C2C267C2226EF596	FASTA	1,654	191,116
	10 20 30 40 50 60 MTDRFWDQWY LWYLRLLRLL DRGSFRNDGL KASDVLPILK EKVAFVSGGR DKRGGPILTF 70 80 90 100 110 120 PARTNHDRIR QEDLRKLVTN LASVPSEDVC KRGFTVIIDM RGSKWDLIKP LLKTLQEAFP 130 140 150 160 170 180 AEIHVALIIK PDNFWQKQKT NFGSSKFIFE TSMVSVEGLT KLVDPSQLTE EFDGSLDYNH 190 200 210 220 230 240 EEWIELRLSL EEFFNSAVHL LSRLEDLQEM LARKEFPVDV EGSRRLIDEH TQLKKKVLKA 250 260 270 280 290 300 PVEELDREGQ RLLQCIRCSD GFSGRNCIPG SADFQSLVPK ITSLLDKLHS TRQHLHQMWH 310 320 330 340 350 360 VRKLKLDQCF QLRLFEQDAE KMFDWISHNK ELFLQSHTEI GVSYQHALDL QTQHNHFAMN 370 380 390 400 410 420 SMNAYVNINR IMSVASRLSE AGHYASQQIK QISTQLDQEW KSFAAALDER STILAMSAVF 430 440 450 460 470 480 HQKAEQFLSG VDAWCKMCSE GGLPSEMQDL ELAIHHHQSL YEQVTQAYTE VSQDGKALLD 490 500 510 520 530 540 VLQRPLSPGN SESLTATANY SKAVHQVLDV VHEVLHHQRR LESIWQHRKV RLHQRLQLCV 550 560 570 580 590 600 FQQDVQQVLD WIENHGEAFL SKHTGVGKSL HRARALQKRH DDFEEVAQNT YTNADKLLEA 610 620 630 640 650 660 AEQLAQTGEC DPEEIYKAAR HLEVRIQDFV RRVEQRKLLL DMSVSFHTHT KELWTWMEDL 670 680 690 700 710 720 QKEVLEDVCA DSVDAVQELI KQFQQQQTAT LDATLNVIKE GEDLIQQLRS APPSLGEPTE 730 740 750 760 770 780 ARDSAVSNNK TPHSSSISHI ESVLQQLDDA QVQMEELFHE RKIKLDIFLQ LRIFEQYTIE 790 800 810 820 830 840 VTAELDAWNE DLLRQMNDFN TEDLTLAEQR LQRHTERKLA MNNMTFEVIQ QGQDLHQYIM 850 860 870 880 890 900 EVQASGIELI CEKDVDLAAQ VQELLEFLHE KQHELELNAE QTHKRLEQCL QLRHLQAEVK 910 920 930 940 950 960 QVLGWIRNGE SMLNASLVNA SSLSEAEQLQ REHEQFQLAI EKTHQSALQV QQKAEALLQA 970 980 990 1000 1010 1020 GHYDADAIRE CAEKVALHWQ QLMLKMEDRL KLVNASVAFY KTSEQVCSVL ESLEQEYRRD 1030 1040 1050 1060 1070 1080 EDWCGGRDKL GPAAEMDHVI PLLSKHLEQK EAFLKACTLA RRNAEVFLKY IHRNNVSMPS 1090 1100 1110 1120 1130 1140 VASHTRGPEQ QVKAILSELL QRENRVLHFW TLKKRRLDQC QQYVVFERSA KQALDWIQET 1150 1160 1170 1180 1190 1200 GEYYLSTHTS TGETTEETQE LLKEYGEFRV PAKQTKEKVK LLIQLADSFV EKGHIHATEI 1210 1220 1230 1240 1250 1260 RKWVTTVDKH YRDFSLRMGK YRYTLEKALG VNTEDNKDLE LDIIPASLSD REVKLRDANH 1270 1280 1290 1300 1310 1320 EVNEEKRKSA RKKEFIMAEL LQTEKAYVRD LHECLETYLW EMTSGVEEIP PGILNKEHII 1330 1340 1350 1360 1370 1380 FGNIQEIYDF HNNIFLKELE KYEQLPEDVG HCFVTWADKF QMYVTYCKNK PDSNQLILEH 1390 1400 1410 1420 1430 1440 AGTFFDEIQQ RHGLANSISS YLIKPVQRVT KYQLLLKELL TCCEEGKGEL KDGLEVMLSV 1450 1460 1470 1480 1490 1500 PKKANDAMHV SMLEGFDENL DVQGELILQD AFQVWDPKSL IRKGRERHLF LFEISLVFSK 1510 1520 1530 1540 1550 1560 EIKDSSGHTK YVYKNKLLTS ELGVTEHVEG DPCKFALWSG RTPSSDNKTV LKASNIETKQ 1570 1580 1590 1600 1610 1620 EWIKNIREVI QERIIHLKGA LKEPIQLPKT PAKLRNNSKR DGVEDGDSQG DGSSQPDTIS 1630 1640 1650 IASRTSQNTV ESDKDGNLVP RWHLGPGDPF STYV
	Isoform 7 (Kalirin-9A). Checksum: 3AEC5B08F483E75C	FASTA	2,376	271,353
	10 20 30 40 50 60 MVLSGSFRND GLKASDVLPI LKEKVAFVSG GRDKRGGPIL TFPARTNHDR IRQEDLRKLV 70 80 90 100 110 120 TNLASVPSED VCKRGFTVII DMRGSKWDLI KPLLKTLQEA FPAEIHVALI IKPDNFWQKQ 130 140 150 160 170 180 KTNFGSSKFI FETSMVSVEG LTKLVDPSQL TEEFDGSLDY NHEEWIELRL SLEEFFNSAV 190 200 210 220 230 240 HLLSRLEDLQ EMLARKEFPV DVEGSRRLID EHTQLKKKVL KAPVEELDRE GQRLLQCIRC 250 260 270 280 290 300 SDGFSGRNCI PGSADFQSLV PKITSLLDKL HSTRQHLHQM WHVRKLKLDQ CFQLRLFEQD 310 320 330 340 350 360 AEKMFDWISH NKELFLQSHT EIGVSYQHAL DLQTQHNHFA MNSMNAYVNI NRIMSVASRL 370 380 390 400 410 420 SEAGHYASQQ IKQISTQLDQ EWKSFAAALD ERSTILAMSA VFHQKAEQFL SGVDAWCKMC 430 440 450 460 470 480 SEGGLPSEMQ DLELAIHHHQ SLYEQVTQAY TEVSQDGKAL LDVLQRPLSP GNSESLTATA 490 500 510 520 530 540 NYSKAVHQVL DVVHEVLHHQ RRLESIWQHR KVRLHQRLQL CVFQQDVQQV LDWIENHGEA 550 560 570 580 590 600 FLSKHTGVGK SLHRARALQK RHDDFEEVAQ NTYTNADKLL EAAEQLAQTG ECDPEEIYKA 610 620 630 640 650 660 ARHLEVRIQD FVRRVEQRKL LLDMSVSFHT HTKELWTWME DLQKEVLEDV CADSVDAVQE 670 680 690 700 710 720 LIKQFQQQQT ATLDATLNVI KEGEDLIQQL RSAPPSLGEP TEARDSAVSN NKTPHSSSIS 730 740 750 760 770 780 HIESVLQQLD DAQVQMEELF HERKIKLDIF LQLRIFEQYT IEVTAELDAW NEDLLRQMND 790 800 810 820 830 840 FNTEDLTLAE QRLQRHTERK LAMNNMTFEV IQQGQDLHQY IMEVQASGIE LICEKDVDLA 850 860 870 880 890 900 AQVQELLEFL HEKQHELELN AEQTHKRLEQ CLQLRHLQAE VKQVLGWIRN GESMLNASLV 910 920 930 940 950 960 NASSLSEAEQ LQREHEQFQL AIEKTHQSAL QVQQKAEALL QAGHYDADAI RECAEKVALH 970 980 990 1000 1010 1020 WQQLMLKMED RLKLVNASVA FYKTSEQVCS VLESLEQEYR RDEDWCGGRD KLGPAAEMDH 1030 1040 1050 1060 1070 1080 VIPLLSKHLE QKEAFLKACT LARRNAEVFL KYIHRNNVSM PSVASHTRGP EQQVKAILSE 1090 1100 1110 1120 1130 1140 LLQRENRVLH FWTLKKRRLD QCQQYVVFER SAKQALDWIQ ETGEYYLSTH TSTGETTEET 1150 1160 1170 1180 1190 1200 QELLKEYGEF RVPAKQTKEK VKLLIQLADS FVEKGHIHAT EIRKWVTTVD KHYRDFSLRM 1210 1220 1230 1240 1250 1260 GKYRYTLEKA LGVNTEDNKD LELDIIPASL SDREVKLRDA NHEVNEEKRK SARKKEFIMA 1270 1280 1290 1300 1310 1320 ELLQTEKAYV RDLHECLETY LWEMTSGVEE IPPGILNKEH IIFGNIQEIY DFHNNIFLKE 1330 1340 1350 1360 1370 1380 LEKYEQLPED VGHCFVTWAD KFQMYVTYCK NKPDSNQLIL EHAGTFFDEI QQRHGLANSI 1390 1400 1410 1420 1430 1440 SSYLIKPVQR VTKYQLLLKE LLTCCEEGKG ELKDGLEVML SVPKKANDAM HVSMLEGFDE 1450 1460 1470 1480 1490 1500 NLDVQGELIL QDAFQVWDPK SLIRKGRERH LFLFEISLVF SKEIKDSSGH TKYVYKNKLL 1510 1520 1530 1540 1550 1560 TSELGVTEHV EGDPCKFALW SGRTPSSDNK TVLKASNIET KQEWIKNIRE VIQERIIHLK 1570 1580 1590 1600 1610 1620 GALKEPIQLP KTPAKLRNNS KRDGVEDGDS QGDGSSQPDT ISIASRTSQN TVESDKLSGG 1630 1640 1650 1660 1670 1680 CELTVVLQDF SAAHSSELSI QVGQTVELLE RPSERPGWCL VRTTERSPPQ EGLVPSSTLC 1690 1700 1710 1720 1730 1740 ISHSRSSVEM DCFFPLVKDS YSHSSGENGG KSESVANLQS QPSLNSIHSS PGPKRSTNTL 1750 1760 1770 1780 1790 1800 KKWLTSPVRR LNSGKADGNI KKQKKVRDGR KSFDLGSPKP GDETTPQGDS ADEKSKKGWG 1810 1820 1830 1840 1850 1860 EDEPDEESHT PLPPPMKIFD NDPTQDEMSS LLAARQAPTD VPTAADLVSA IEKLVKSKLT 1870 1880 1890 1900 1910 1920 LEGGSYRGSL KDPTVCLNEG MAPPTPPRNL EEEQKAKALR GRMFVLNELV QTEKDYVKDL 1930 1940 1950 1960 1970 1980 GIVVEGFMKR IEEKGVPEDM RGKEKIVFGN IHQIYDWHKD FFLAELEKCI QEQDRLAQLF 1990 2000 2010 2020 2030 2040 IKHERKLHIY VWYCQNKPRS EYIVAEYDAY FEEVKQEINQ RLTLSDFLIK PIQRITKYQL 2050 2060 2070 2080 2090 2100 LLKDFLRYSE KAGLECSDIE KAVELMCLVP KRCNDMMNLG RLQGFEGTLT AQGKLLQQDT 2110 2120 2130 2140 2150 2160 FYVIELDAGM QSRTKERRVF LFEQIVIFSE LLRKGSLTPG YMFKRSIKMN YLVLEEDVDD 2170 2180 2190 2200 2210 2220 DPCKFALMNR ETSERVILQA ANSDIQQAWV QDINQVLETQ RDFLNALQSP IEYQRKERST 2230 2240 2250 2260 2270 2280 AVIRSQPPRV PQASPRPYSS VPVGSEKPPK GSSYNPPLPP LKISTSNGSP GFDYHQPGDK 2290 2300 2310 2320 2330 2340 FDASKQNDLG GCNGTSTMAV IKDYYALKEN EICVSQGEVV QVLAVNQQNM CLVYQPASDH 2350 2360 2370 SPAAEGWVPG SILAPLTKAT APAESSDESI KTLLKP
	Isoform 8. Checksum: 08AB2C8397ED9654	FASTA	717	81,973
	10 20 30 40 50 60 MVLSGSFRND GLKASDVLPI LKEKVAFVSG GRDKRGGPIL TFPARTNHDR IRQEDLRKLV 70 80 90 100 110 120 TNLASVPSED VCKRGFTVII DMRGSKWDLI KPLLKTLQEA FPAEIHVALI IKPDNFWQKQ 130 140 150 160 170 180 KTNFGSSKFI FETSMVSVEG LTKLVDPSQL TEEFDGSLDY NHEEWIELRL SLEEFFNSAV 190 200 210 220 230 240 HLLSRLEDLQ EMLARKEFPV DVEGSRRLID EHTQLKKKVL KAPVEELDRE GQRLLQCIRC 250 260 270 280 290 300 SDGFSGRNCI PGSADFQSLV PKITSLLDKL HSTRQHLHQM WHVRKLKLDQ CFQLRLFEQD 310 320 330 340 350 360 AEKMFDWISH NKELFLQSHT EIGVSYQHAL DLQTQHNHFA MNSMNAYVNI NRIMSVASRL 370 380 390 400 410 420 SEAGHYASQQ IKQISTQLDQ EWKSFAAALD ERSTILAMSA VFHQKAEQFL SGVDAWCKMC 430 440 450 460 470 480 SEGGLPSEMQ DLELAIHHHQ SLYEQVTQAY TEVSQDGKAL LDVLQRPLSP GNSESLTATA 490 500 510 520 530 540 NYSKAVHQVL DVVHEVLHHQ RRLESIWQHR KVRLHQRLQL CVFQQDVQQV LDWIENHGEA 550 560 570 580 590 600 FLSKHTGVGK SLHRARALQK RHDDFEEVAQ NTYTNADKLL EAAEQLAQTG ECDPEEIYKA 610 620 630 640 650 660 ARHLEVRIQD FVRRVEQRKL LLDMSVSFHT HTKELWTWME DLQKEVLEDV CADSVDAVQE 670 680 690 700 710 LIKQFQQQQT ATLDATLNVI KEGEDLIQQL RSAPPSLGEP TEARSAWAVI PVGNASG

References

[1]	"Novel proteins that interact with the COOH-terminal cytosolic routing determinants of an integral membrane peptide-processing enzyme." Alam M.R., Caldwell B.D., Johnson R.C., Darlington D.N., Mains R.E., Eipper B.A. J. Biol. Chem. 271:28636-28640(1996) [PubMed: 8910496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Hippocampus.
[2]	"Kalirin, a cytosolic protein with spectrin-like and GDP/GTP exchange factor-like domains that interacts with peptidylglycine alpha-amidating monooxygenase, an integral membrane peptide-processing enzyme." Alam M.R., Johnson R.C., Darlington D.N., Hand T.A., Mains R.E., Eipper B.A. J. Biol. Chem. 272:12667-12675(1997) [PubMed: 9139723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), INTERACTION WITH PAM. Tissue: Hippocampus.
[3]	"An isoform of kalirin, a brain-specific GDP/GTP exchange factor, is enriched in the postsynaptic density fraction." Penzes P., Johnson R.C., Alam M.R., Kambampati V., Mains R.E., Eipper B.A. J. Biol. Chem. 275:6395-6403(2000) [PubMed: 10692441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"Isoforms of kalirin, a neuronal Dbl family member, generated through use of different 5'- and 3'-ends along with an internal translational initiation site." Johnson R.C., Penzes P., Eipper B.A., Mains R.E. J. Biol. Chem. 275:19324-19333(2000) [PubMed: 10777487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 5 AND 7), ALTERNATIVE INITIATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Induction of lamellipodia by Kalirin does not require its guanine nucleotide exchange factor activity." Schiller M.R., Blangy A., Huang J., Mains R.E., Eipper B.A. Exp. Cell Res. 307:402-417(2005) [PubMed: 15950621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 8), FUNCTION.
[6]	"Huntingtin-associated protein 1 (HAP1) binds to a Trio-like polypeptide, with a rac1 guanine nucleotide exchange factor domain." Colomer V., Engelender S., Sharp A.H., Duan K., Cooper J.K., Lanahan A., Lyford G., Worley P., Ross C.A. Hum. Mol. Genet. 6:1519-1525(1997) [PubMed: 9285789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 638-1216 (ISOFORM 4). Tissue: Brain cortex and Hippocampus.
[7]	"Distinct roles for the two Rho GDP/GTP exchange factor domains of kalirin in regulation of neurite growth and neuronal morphology." Penzes P., Johnson R.C., Kambampati V., Mains R.E., Eipper B.A. J. Neurosci. 21:8426-8434(2001) [PubMed: 11606631] [Abstract] Cited for: FUNCTION, DOMAIN, DEVELOPMENTAL STAGE.
[8]	"Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions." Schiller M.R., Chakrabarti K., King G.F., Schiller N.I., Eipper B.A., Maciejewski M.W. J. Biol. Chem. 281:18774-18786(2006) [PubMed: 16644733] [Abstract] Cited for: STRUCTURE BY NMR OF 1617-1707, DOMAIN, INTERACTION WITH CRK1.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U88156 mRNA. Translation: AAB66366.1.
U88157 mRNA. Translation: AAB66367.1.
AF230644 mRNA. Translation: AAF69144.1.
AF229255 mRNA. Translation: AAF66014.1.
AF232668 mRNA. Translation: AAF66018.1.
AF232669 mRNA. Translation: AAF66019.1.
AY621095 mRNA. Translation: AAT39517.1.
U94189 mRNA. Translation: AAC15790.1.

IPI

IPI00200504.
IPI00400488.
IPI00476515.
IPI00567732.
IPI00869510.
IPI00869788.
IPI00870072.
IPI00870649.

PIR

T32732.
T42098.

RefSeq

NP_114451.1.

UniGene

Rn.87882

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1U3O	NMR	-	A	1617-1686	[»]

SMR

P97924. Positions 1272-1575.

ModBase

Search...

Protein-protein interaction databases

IntAct

P97924. 2 interactions.

PTM databases

PhosphoSite

P97924.

Proteomic databases

PRIDE

P97924.

Genome annotation databases

Ensembl

ENSRNOG00000001706. Rattus norvegicus. [Contig view]

GeneID

84009.

KEGG

rno:84009.

Organism-specific databases

RGD

621865. Hapip.

Phylogenomic databases

HOVERGEN

P97924.

Enzyme and pathway databases

BRENDA

2.7.11.1. 248.

Gene expression databases

ArrayExpress

P97924.

GermOnline

ENSRNOG00000001706. Rattus norvegicus.

Family and domain databases

InterPro

IPR001251. CRAL_bd_TRIO_C.
IPR000219. DH-domain.
IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR001331. GDS_CDC24_CS.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]

Gene3D

G3DSA:3.40.525.10. CRAL_bd_TRIO_C. 1 hit.
G3DSA:2.60.40.30. FN_III-like. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
G3DSA:2.30.29.30. PH_type. 2 hits.
G3DSA:1.20.900.10. RhoGEF. 2 hits.

Pfam

PF00041. fn3. 1 hit.
PF07679. I-set. 1 hit.
PF00169. PH. 2 hits.
PF00069. Pkinase. 1 hit.
PF00621. RhoGEF. 2 hits.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
PF00435. Spectrin. 5 hits.
[Graphical view]

ProDom

PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00060. FN3. 1 hit.
SM00408. IGc2. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 2 hits.
SM00220. S_TKc. 1 hit.
SM00516. SEC14. 1 hit.
SM00326. SH3. 2 hits.
SM00150. SPEC. 7 hits.
[Graphical view]

PROSITE

PS50191. CRAL_TRIO. 1 hit.
PS00741. DH_1. False negative.
PS50010. DH_2. 2 hits.
PS50853. FN3. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

616533.

Entry information

Entry name

KALRN_RAT

Accession

Primary (citable) accession number: P97924
Secondary accession number(s): O70135 , Q6IV51, Q9JIF1, Q9JIF2, Q9JIG0, Q9JIH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)