Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P97874

- GAK_RAT

UniProt

P97874 - GAK_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cyclin-G-associated kinase

Gene

Gak

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin binding Source: RGD
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-G-associated kinase (EC:2.7.11.1)
Gene namesi
Name:Gak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621589. Gak.

Subcellular locationi

Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity
Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions (By similarity).By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 13051304Cyclin-G-associated kinasePRO_0000085960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei454 – 4541PhosphoserineBy similarity
Modified residuei768 – 7681PhosphoserineBy similarity
Modified residuei824 – 8241PhosphoserineBy similarity
Modified residuei827 – 8271PhosphoserineBy similarity
Modified residuei938 – 9381PhosphoserineBy similarity
Modified residuei1171 – 11711PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP97874.
PRIDEiP97874.

PTM databases

PhosphoSiteiP97874.

Expressioni

Gene expression databases

GenevestigatoriP97874.

Interactioni

Protein-protein interaction databases

IntActiP97874. 1 interaction.
MINTiMINT-3289568.
STRINGi10116.ENSRNOP00000000064.

Structurei

3D structure databases

ProteinModelPortaliP97874.
SMRiP97874. Positions 1132-1305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 315276Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini397 – 564168Phosphatase tensin-typePROSITE-ProRule annotationAdd
BLAST
Domaini570 – 708139C2 tensin-typePROSITE-ProRule annotationAdd
BLAST
Domaini1241 – 130565JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation
Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiP97874.
KOiK08855.
PhylomeDBiP97874.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97874-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG
60 70 80 90 100
FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ
110 120 130 140 150
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLRRVECK GPLSCDSILK
160 170 180 190 200
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH
210 220 230 240 250
YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG
260 270 280 290 300
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRGMLKVNP
310 320 330 340 350
EERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPS
360 370 380 390 400
GGPVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN
410 420 430 440 450
YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRLFL DAKHPGHYAV
460 470 480 490 500
YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN
510 520 530 540 550
VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH
560 570 580 590 600
KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY
610 620 630 640 650
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GITVQGDVLT IIYHARSTLG
660 670 680 690 700
GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ
710 720 730 740 750
VNLEVEVEPR DRPSRDVPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP
760 770 780 790 800
ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKD
810 820 830 840 850
PETGVDNTSP KESQSNLIAD GDGSEVSDEE EASCPSEERK PGAGEDTPRL
860 870 880 890 900
AAGTRQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQASG
910 920 930 940 950
VQSSNTDLLS SLLEPSDASQ VGPPGDLLGG ETPLLLASPV SLLGVQSNLQ
960 970 980 990 1000
GKVPDTVDPF DQFLLPSSSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS
1010 1020 1030 1040 1050
APPPSCSTAF LHLGDLPAEP NKVIASSSHP DLLGGWDTWA ETALPGPASM
1060 1070 1080 1090 1100
PVPEGTLFSS AGHPAPPGPN PSQTKSQNPD PFADLSDLSS SLQGLPAGLP
1110 1120 1130 1140 1150
AGSFVGTSAT THKSNSSWQT TRPTAPGTSW PPQAKPAPRA SEQLRSHFSV
1160 1170 1180 1190 1200
IGAREERGVR APSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK
1210 1220 1230 1240 1250
QELARDTDPF KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD
1260 1270 1280 1290 1300
LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ SAKMIFMELN DAWSEFENQG

SRPLF
Length:1,305
Mass (Da):143,703
Last modified:May 1, 1997 - v1
Checksum:i6D36BD38011C44EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38560 mRNA. Translation: BAA18911.1.
PIRiT31096.
RefSeqiNP_112292.1. NM_031030.2.
UniGeneiRn.11012.

Genome annotation databases

GeneIDi81659.
KEGGirno:81659.
UCSCiRGD:621589. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38560 mRNA. Translation: BAA18911.1 .
PIRi T31096.
RefSeqi NP_112292.1. NM_031030.2.
UniGenei Rn.11012.

3D structure databases

ProteinModelPortali P97874.
SMRi P97874. Positions 1132-1305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97874. 1 interaction.
MINTi MINT-3289568.
STRINGi 10116.ENSRNOP00000000064.

PTM databases

PhosphoSitei P97874.

Proteomic databases

PaxDbi P97874.
PRIDEi P97874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81659.
KEGGi rno:81659.
UCSCi RGD:621589. rat.

Organism-specific databases

CTDi 2580.
RGDi 621589. Gak.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000034235.
HOVERGENi HBG004322.
InParanoidi P97874.
KOi K08855.
PhylomeDBi P97874.

Miscellaneous databases

NextBioi 615222.
PROi P97874.

Gene expression databases

Genevestigatori P97874.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProi IPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view ]
Pfami PF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view ]
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain."
    Kanaoka Y., Kimura S.H., Okazaki I., Ikeda M., Nojima H.
    FEBS Lett. 402:73-80(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiGAK_RAT
AccessioniPrimary (citable) accession number: P97874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3