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P97874

- GAK_RAT

UniProt

P97874 - GAK_RAT

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Protein
Cyclin-G-associated kinase
Gene
Gak
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731Proton acceptor By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin binding Source: RGD
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-G-associated kinase (EC:2.7.11.1)
Gene namesi
Name:Gak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621589. Gak.

Subcellular locationi

Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity
Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. focal adhesion Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 13051304Cyclin-G-associated kinase
PRO_0000085960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei16 – 161Phosphoserine By similarity
Modified residuei454 – 4541Phosphoserine By similarity
Modified residuei768 – 7681Phosphoserine By similarity
Modified residuei824 – 8241Phosphoserine By similarity
Modified residuei827 – 8271Phosphoserine By similarity
Modified residuei938 – 9381Phosphoserine By similarity
Modified residuei1171 – 11711Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP97874.
PRIDEiP97874.

PTM databases

PhosphoSiteiP97874.

Expressioni

Gene expression databases

GenevestigatoriP97874.

Interactioni

Protein-protein interaction databases

IntActiP97874. 1 interaction.
MINTiMINT-3289568.
STRINGi10116.ENSRNOP00000000064.

Structurei

3D structure databases

ProteinModelPortaliP97874.
SMRiP97874. Positions 1132-1305.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 315276Protein kinase
Add
BLAST
Domaini397 – 564168Phosphatase tensin-type
Add
BLAST
Domaini570 – 708139C2 tensin-type
Add
BLAST
Domaini1241 – 130565J
Add
BLAST

Sequence similaritiesi

Contains 1 J domain.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiP97874.
KOiK08855.
PhylomeDBiP97874.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97874-1 [UniParc]FASTAAdd to Basket

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MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG     50
FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ 100
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLRRVECK GPLSCDSILK 150
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH 200
YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG 250
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRGMLKVNP 300
EERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPS 350
GGPVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN 400
YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRLFL DAKHPGHYAV 450
YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN 500
VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH 550
KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 600
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GITVQGDVLT IIYHARSTLG 650
GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ 700
VNLEVEVEPR DRPSRDVPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP 750
ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKD 800
PETGVDNTSP KESQSNLIAD GDGSEVSDEE EASCPSEERK PGAGEDTPRL 850
AAGTRQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQASG 900
VQSSNTDLLS SLLEPSDASQ VGPPGDLLGG ETPLLLASPV SLLGVQSNLQ 950
GKVPDTVDPF DQFLLPSSSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS 1000
APPPSCSTAF LHLGDLPAEP NKVIASSSHP DLLGGWDTWA ETALPGPASM 1050
PVPEGTLFSS AGHPAPPGPN PSQTKSQNPD PFADLSDLSS SLQGLPAGLP 1100
AGSFVGTSAT THKSNSSWQT TRPTAPGTSW PPQAKPAPRA SEQLRSHFSV 1150
IGAREERGVR APSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 1200
QELARDTDPF KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD 1250
LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ SAKMIFMELN DAWSEFENQG 1300
SRPLF 1305
Length:1,305
Mass (Da):143,703
Last modified:May 1, 1997 - v1
Checksum:i6D36BD38011C44EE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38560 mRNA. Translation: BAA18911.1.
PIRiT31096.
RefSeqiNP_112292.1. NM_031030.2.
UniGeneiRn.11012.

Genome annotation databases

GeneIDi81659.
KEGGirno:81659.
UCSCiRGD:621589. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38560 mRNA. Translation: BAA18911.1 .
PIRi T31096.
RefSeqi NP_112292.1. NM_031030.2.
UniGenei Rn.11012.

3D structure databases

ProteinModelPortali P97874.
SMRi P97874. Positions 1132-1305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97874. 1 interaction.
MINTi MINT-3289568.
STRINGi 10116.ENSRNOP00000000064.

PTM databases

PhosphoSitei P97874.

Proteomic databases

PaxDbi P97874.
PRIDEi P97874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 81659.
KEGGi rno:81659.
UCSCi RGD:621589. rat.

Organism-specific databases

CTDi 2580.
RGDi 621589. Gak.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000034235.
HOVERGENi HBG004322.
InParanoidi P97874.
KOi K08855.
PhylomeDBi P97874.

Miscellaneous databases

NextBioi 615222.
PROi P97874.

Gene expression databases

Genevestigatori P97874.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProi IPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view ]
Pfami PF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view ]
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain."
    Kanaoka Y., Kimura S.H., Okazaki I., Ikeda M., Nojima H.
    FEBS Lett. 402:73-80(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiGAK_RAT
AccessioniPrimary (citable) accession number: P97874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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