Cyclin-G-associated kinase - Rattus norvegicus (Rat)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Cyclin-G-associated kinase
EC=2.7.11.1

Gene names

Name:

Gak

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm › perinuclear region By similarity. Golgi apparatus › trans-Golgi network By similarity. Cell junction › focal adhesion By similarity. Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 C2 tensin-type domain. Contains 1 J domain. Contains 1 phosphatase tensin-type domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cell junction Cytoplasm Golgi apparatus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin binding Inferred from direct assay Ref.1. Source: RGD protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed By similarity

Chain

2 – 1305

1304

Cyclin-G-associated kinase

PRO_0000085960

Regions

Domain

40 – 315

276

Protein kinase

Domain

397 – 564

168

Phosphatase tensin-type

Domain

570 – 708

139

C2 tensin-type

Domain

1241 – 1305

65

J

Sites

Active site

173

1

Proton acceptor By similarity

Amino acid modifications

Modified residue

2

1

N-acetylserine By similarity

Modified residue

16

1

Phosphoserine By similarity

Modified residue

454

1

Phosphoserine By similarity

Modified residue

768

1

Phosphoserine By similarity

Modified residue

824

1

Phosphoserine By similarity

Modified residue

827

1

Phosphoserine By similarity

Modified residue

938

1

Phosphoserine By similarity

Modified residue

1171

1

Phosphoserine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P97874 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 6D36BD38011C44EE
Show »

FASTA

1,305

143,703

        10         20         30         40         50         60 
MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG FAFVYEAQDL 

        70         80         90        100        110        120 
GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ FCSAASIGKE ESDTGQAEFL 

       130        140        150        160        170        180 
LLTELCKGQL VEFLRRVECK GPLSCDSILK IFYQTCRAVQ HMHRQKPPII HRDLKVENLL 

       190        200        210        220        230        240 
LSNQGTIKLC DFGSATTISH YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI 

       250        260        270        280        290        300 
GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRGMLKVNP 

       310        320        330        340        350        360 
EERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPS GGPVNSSGVL 

       370        380        390        400        410        420 
ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN YAKGDLDISY ITSRIAVMSF 

       430        440        450        460        470        480 
PAEGVESAIK NNIEDVRLFL DAKHPGHYAV YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL 

       490        500        510        520        530        540 
HSLYTLCRSM HAWLREDHRN VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK 

       550        560        570        580        590        600 
RCPPGIWPSH KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 

       610        620        630        640        650        660 
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GITVQGDVLT IIYHARSTLG GRLQAKMASM 

       670        680        690        700        710        720 
KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ VNLEVEVEPR DRPSRDVPPW 

       730        740        750        760        770        780 
ENTSLRGLNP KILFSNREEQ QDILSKFGKP ELPRQPGSTA QYDAEAGSPE AEITESDSPQ 

       790        800        810        820        830        840 
SSSTDTNHFL HTLDWQEEKD PETGVDNTSP KESQSNLIAD GDGSEVSDEE EASCPSEERK 

       850        860        870        880        890        900 
PGAGEDTPRL AAGTRQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQASG 

       910        920        930        940        950        960 
VQSSNTDLLS SLLEPSDASQ VGPPGDLLGG ETPLLLASPV SLLGVQSNLQ GKVPDTVDPF 

       970        980        990       1000       1010       1020 
DQFLLPSSSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS APPPSCSTAF LHLGDLPAEP 

      1030       1040       1050       1060       1070       1080 
NKVIASSSHP DLLGGWDTWA ETALPGPASM PVPEGTLFSS AGHPAPPGPN PSQTKSQNPD 

      1090       1100       1110       1120       1130       1140 
PFADLSDLSS SLQGLPAGLP AGSFVGTSAT THKSNSSWQT TRPTAPGTSW PPQAKPAPRA 

      1150       1160       1170       1180       1190       1200 
SEQLRSHFSV IGAREERGVR APSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 

      1210       1220       1230       1240       1250       1260 
QELARDTDPF KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD LVTPEQVKKQ 

      1270       1280       1290       1300 
YRRAVLVVHP DKATGQPYEQ SAKMIFMELN DAWSEFENQG SRPLF

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References

[1]	"GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain." Kanaoka Y., Kimura S.H., Okazaki I., Ikeda M., Nojima H. FEBS Lett. 402:73-80(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38560 mRNA. Translation: BAA18911.1.
IPI	IPI00208824.
PIR	T31096.
RefSeq	NP_112292.1. NM_031030.2.
UniGene	Rn.11012.
3D structure databases
ProteinModelPortal	P97874.
SMR	P97874. Positions 1132-1305.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3289568.
STRING	10116.ENSRNOP00000000064.
PTM databases
PhosphoSite	P97874.
Proteomic databases
PaxDb	P97874.
PRIDE	P97874.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81659.
KEGG	rno:81659.
UCSC	RGD:621589. rat.
Organism-specific databases
CTD	2580.
RGD	621589. Gak.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000034235.
HOVERGEN	HBG004322.
InParanoid	P97874.
KO	K08855.
OrthoDB	EOG44MXR7.
Gene expression databases
ArrayExpress	P97874.
Genevestigator	P97874.
GermOnline	ENSRNOG00000000048. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR001623. DnaJ_domain. IPR011009. Kinase-like_dom. IPR014019. Phosphatase_tensin-typ. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. IPR014020. Tensin_phosphatase_C2-dom. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. PF00069. Pkinase. 1 hit. PF10409. PTEN_C2. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF46565. DnaJ_N. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51182. C2_TENSIN. 1 hit. PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. PS51181. PPASE_TENSIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615222.

Entry information

Entry name

GAK_RAT

Accession

Primary (citable) accession number: P97874

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents