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P97874 (GAK_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-G-associated kinase

EC=2.7.11.1
Gene names
Name:Gak
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity. Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 C2 tensin-type domain.

Contains 1 J domain.

Contains 1 phosphatase tensin-type domain.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 13051304Cyclin-G-associated kinase
PRO_0000085960

Regions

Domain40 – 315276Protein kinase
Domain397 – 564168Phosphatase tensin-type
Domain570 – 708139C2 tensin-type
Domain1241 – 130565J

Sites

Active site1731Proton acceptor By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue161Phosphoserine By similarity
Modified residue4541Phosphoserine By similarity
Modified residue7681Phosphoserine By similarity
Modified residue8241Phosphoserine By similarity
Modified residue8271Phosphoserine By similarity
Modified residue9381Phosphoserine By similarity
Modified residue11711Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P97874 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 6D36BD38011C44EE

FASTA1,305143,703
        10         20         30         40         50         60 
MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG FAFVYEAQDL 

        70         80         90        100        110        120 
GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ FCSAASIGKE ESDTGQAEFL 

       130        140        150        160        170        180 
LLTELCKGQL VEFLRRVECK GPLSCDSILK IFYQTCRAVQ HMHRQKPPII HRDLKVENLL 

       190        200        210        220        230        240 
LSNQGTIKLC DFGSATTISH YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI 

       250        260        270        280        290        300 
GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRGMLKVNP 

       310        320        330        340        350        360 
EERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPS GGPVNSSGVL 

       370        380        390        400        410        420 
ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN YAKGDLDISY ITSRIAVMSF 

       430        440        450        460        470        480 
PAEGVESAIK NNIEDVRLFL DAKHPGHYAV YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL 

       490        500        510        520        530        540 
HSLYTLCRSM HAWLREDHRN VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK 

       550        560        570        580        590        600 
RCPPGIWPSH KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 

       610        620        630        640        650        660 
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GITVQGDVLT IIYHARSTLG GRLQAKMASM 

       670        680        690        700        710        720 
KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ VNLEVEVEPR DRPSRDVPPW 

       730        740        750        760        770        780 
ENTSLRGLNP KILFSNREEQ QDILSKFGKP ELPRQPGSTA QYDAEAGSPE AEITESDSPQ 

       790        800        810        820        830        840 
SSSTDTNHFL HTLDWQEEKD PETGVDNTSP KESQSNLIAD GDGSEVSDEE EASCPSEERK 

       850        860        870        880        890        900 
PGAGEDTPRL AAGTRQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQASG 

       910        920        930        940        950        960 
VQSSNTDLLS SLLEPSDASQ VGPPGDLLGG ETPLLLASPV SLLGVQSNLQ GKVPDTVDPF 

       970        980        990       1000       1010       1020 
DQFLLPSSSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS APPPSCSTAF LHLGDLPAEP 

      1030       1040       1050       1060       1070       1080 
NKVIASSSHP DLLGGWDTWA ETALPGPASM PVPEGTLFSS AGHPAPPGPN PSQTKSQNPD 

      1090       1100       1110       1120       1130       1140 
PFADLSDLSS SLQGLPAGLP AGSFVGTSAT THKSNSSWQT TRPTAPGTSW PPQAKPAPRA 

      1150       1160       1170       1180       1190       1200 
SEQLRSHFSV IGAREERGVR APSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 

      1210       1220       1230       1240       1250       1260 
QELARDTDPF KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD LVTPEQVKKQ 

      1270       1280       1290       1300 
YRRAVLVVHP DKATGQPYEQ SAKMIFMELN DAWSEFENQG SRPLF 

« Hide

References

[1]"GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain."
Kanaoka Y., Kimura S.H., Okazaki I., Ikeda M., Nojima H.
FEBS Lett. 402:73-80(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D38560 mRNA. Translation: BAA18911.1.
PIRT31096.
RefSeqNP_112292.1. NM_031030.2.
UniGeneRn.11012.

3D structure databases

ProteinModelPortalP97874.
SMRP97874. Positions 1132-1305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP97874. 1 interaction.
MINTMINT-3289568.
STRING10116.ENSRNOP00000000064.

PTM databases

PhosphoSiteP97874.

Proteomic databases

PaxDbP97874.
PRIDEP97874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID81659.
KEGGrno:81659.
UCSCRGD:621589. rat.

Organism-specific databases

CTD2580.
RGD621589. Gak.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000034235.
HOVERGENHBG004322.
InParanoidP97874.
KOK08855.
PhylomeDBP97874.

Gene expression databases

GenevestigatorP97874.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamPF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615222.
PROP97874.

Entry information

Entry nameGAK_RAT
AccessionPrimary (citable) accession number: P97874
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families