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P97874

- GAK_RAT

UniProt

P97874 - GAK_RAT

Protein

Cyclin-G-associated kinase

Gene

Gak

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    • Comment

    Functioni

    Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei173 – 1731Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin binding Source: RGD
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-G-associated kinase (EC:2.7.11.1)
    Gene namesi
    Name:Gak
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621589. Gak.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity
    Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.By similarity

    GO - Cellular componenti

    1. focal adhesion Source: UniProtKB-SubCell
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 13051304Cyclin-G-associated kinasePRO_0000085960Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei16 – 161PhosphoserineBy similarity
    Modified residuei454 – 4541PhosphoserineBy similarity
    Modified residuei768 – 7681PhosphoserineBy similarity
    Modified residuei824 – 8241PhosphoserineBy similarity
    Modified residuei827 – 8271PhosphoserineBy similarity
    Modified residuei938 – 9381PhosphoserineBy similarity
    Modified residuei1171 – 11711PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP97874.
    PRIDEiP97874.

    PTM databases

    PhosphoSiteiP97874.

    Expressioni

    Gene expression databases

    GenevestigatoriP97874.

    Interactioni

    Protein-protein interaction databases

    IntActiP97874. 1 interaction.
    MINTiMINT-3289568.
    STRINGi10116.ENSRNOP00000000064.

    Structurei

    3D structure databases

    ProteinModelPortaliP97874.
    SMRiP97874. Positions 1132-1305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 315276Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini397 – 564168Phosphatase tensin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini570 – 708139C2 tensin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini1241 – 130565JPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
    Contains 1 J domain.PROSITE-ProRule annotation
    Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000034235.
    HOVERGENiHBG004322.
    InParanoidiP97874.
    KOiK08855.
    PhylomeDBiP97874.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR011009. Kinase-like_dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view]
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97874-1 [UniParc]FASTAAdd to Basket

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    MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG     50
    FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ 100
    FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLRRVECK GPLSCDSILK 150
    IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH 200
    YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG 250
    CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRGMLKVNP 300
    EERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPS 350
    GGPVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN 400
    YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRLFL DAKHPGHYAV 450
    YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN 500
    VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH 550
    KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 600
    VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GITVQGDVLT IIYHARSTLG 650
    GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ 700
    VNLEVEVEPR DRPSRDVPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP 750
    ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKD 800
    PETGVDNTSP KESQSNLIAD GDGSEVSDEE EASCPSEERK PGAGEDTPRL 850
    AAGTRQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQASG 900
    VQSSNTDLLS SLLEPSDASQ VGPPGDLLGG ETPLLLASPV SLLGVQSNLQ 950
    GKVPDTVDPF DQFLLPSSSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS 1000
    APPPSCSTAF LHLGDLPAEP NKVIASSSHP DLLGGWDTWA ETALPGPASM 1050
    PVPEGTLFSS AGHPAPPGPN PSQTKSQNPD PFADLSDLSS SLQGLPAGLP 1100
    AGSFVGTSAT THKSNSSWQT TRPTAPGTSW PPQAKPAPRA SEQLRSHFSV 1150
    IGAREERGVR APSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 1200
    QELARDTDPF KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD 1250
    LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ SAKMIFMELN DAWSEFENQG 1300
    SRPLF 1305
    Length:1,305
    Mass (Da):143,703
    Last modified:May 1, 1997 - v1
    Checksum:i6D36BD38011C44EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38560 mRNA. Translation: BAA18911.1.
    PIRiT31096.
    RefSeqiNP_112292.1. NM_031030.2.
    UniGeneiRn.11012.

    Genome annotation databases

    GeneIDi81659.
    KEGGirno:81659.
    UCSCiRGD:621589. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38560 mRNA. Translation: BAA18911.1 .
    PIRi T31096.
    RefSeqi NP_112292.1. NM_031030.2.
    UniGenei Rn.11012.

    3D structure databases

    ProteinModelPortali P97874.
    SMRi P97874. Positions 1132-1305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97874. 1 interaction.
    MINTi MINT-3289568.
    STRINGi 10116.ENSRNOP00000000064.

    PTM databases

    PhosphoSitei P97874.

    Proteomic databases

    PaxDbi P97874.
    PRIDEi P97874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81659.
    KEGGi rno:81659.
    UCSCi RGD:621589. rat.

    Organism-specific databases

    CTDi 2580.
    RGDi 621589. Gak.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000034235.
    HOVERGENi HBG004322.
    InParanoidi P97874.
    KOi K08855.
    PhylomeDBi P97874.

    Miscellaneous databases

    NextBioi 615222.
    PROi P97874.

    Gene expression databases

    Genevestigatori P97874.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR011009. Kinase-like_dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view ]
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain."
      Kanaoka Y., Kimura S.H., Okazaki I., Ikeda M., Nojima H.
      FEBS Lett. 402:73-80(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiGAK_RAT
    AccessioniPrimary (citable) accession number: P97874
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3