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Protein

Cyclin-G-associated kinase

Gene

Gak

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin binding Source: RGD
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-G-associated kinase (EC:2.7.11.1)
Gene namesi
Name:Gak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621589 Gak

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000859602 – 1305Cyclin-G-associated kinaseAdd BLAST1304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei454PhosphoserineBy similarity1
Modified residuei768PhosphoserineBy similarity1
Modified residuei774PhosphothreonineBy similarity1
Modified residuei781PhosphoserineBy similarity1
Modified residuei792PhosphothreonineBy similarity1
Modified residuei809PhosphoserineCombined sources1
Modified residuei824PhosphoserineCombined sources1
Modified residuei827PhosphoserineCombined sources1
Modified residuei938PhosphoserineBy similarity1
Modified residuei1122Omega-N-methylarginineBy similarity1
Modified residuei1171PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP97874
PRIDEiP97874

PTM databases

iPTMnetiP97874
PhosphoSitePlusiP97874

Interactioni

GO - Molecular functioni

  • cyclin binding Source: RGD

Protein-protein interaction databases

IntActiP97874, 1 interactor
MINTiP97874
STRINGi10116.ENSRNOP00000000064

Structurei

3D structure databases

ProteinModelPortaliP97874
SMRiP97874
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 315Protein kinasePROSITE-ProRule annotationAdd BLAST276
Domaini397 – 564Phosphatase tensin-typePROSITE-ProRule annotationAdd BLAST168
Domaini570 – 708C2 tensin-typePROSITE-ProRule annotationAdd BLAST139
Domaini1241 – 1305JPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0431 Eukaryota
KOG1989 Eukaryota
KOG2283 Eukaryota
ENOG410Y515 LUCA
HOGENOMiHOG000034235
HOVERGENiHBG004322
InParanoidiP97874
KOiK08855
PhylomeDBiP97874

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR011009 Kinase-like_dom_sf
IPR029021 Prot-tyrosine_phosphatase-like
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
IPR014020 Tensin_C2-dom
IPR029023 Tensin_phosphatase
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF00069 Pkinase, 1 hit
PF10409 PTEN_C2, 1 hit
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM01326 PTEN_C2, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF52799 SSF52799, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51182 C2_TENSIN, 1 hit
PS50076 DNAJ_2, 1 hit
PS51181 PPASE_TENSIN, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG
60 70 80 90 100
FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ
110 120 130 140 150
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLRRVECK GPLSCDSILK
160 170 180 190 200
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH
210 220 230 240 250
YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG
260 270 280 290 300
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRGMLKVNP
310 320 330 340 350
EERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPS
360 370 380 390 400
GGPVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN
410 420 430 440 450
YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRLFL DAKHPGHYAV
460 470 480 490 500
YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN
510 520 530 540 550
VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH
560 570 580 590 600
KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY
610 620 630 640 650
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GITVQGDVLT IIYHARSTLG
660 670 680 690 700
GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ
710 720 730 740 750
VNLEVEVEPR DRPSRDVPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP
760 770 780 790 800
ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKD
810 820 830 840 850
PETGVDNTSP KESQSNLIAD GDGSEVSDEE EASCPSEERK PGAGEDTPRL
860 870 880 890 900
AAGTRQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQASG
910 920 930 940 950
VQSSNTDLLS SLLEPSDASQ VGPPGDLLGG ETPLLLASPV SLLGVQSNLQ
960 970 980 990 1000
GKVPDTVDPF DQFLLPSSSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS
1010 1020 1030 1040 1050
APPPSCSTAF LHLGDLPAEP NKVIASSSHP DLLGGWDTWA ETALPGPASM
1060 1070 1080 1090 1100
PVPEGTLFSS AGHPAPPGPN PSQTKSQNPD PFADLSDLSS SLQGLPAGLP
1110 1120 1130 1140 1150
AGSFVGTSAT THKSNSSWQT TRPTAPGTSW PPQAKPAPRA SEQLRSHFSV
1160 1170 1180 1190 1200
IGAREERGVR APSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK
1210 1220 1230 1240 1250
QELARDTDPF KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD
1260 1270 1280 1290 1300
LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ SAKMIFMELN DAWSEFENQG

SRPLF
Length:1,305
Mass (Da):143,703
Last modified:May 1, 1997 - v1
Checksum:i6D36BD38011C44EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38560 mRNA Translation: BAA18911.1
PIRiT31096
RefSeqiNP_112292.1, NM_031030.2
UniGeneiRn.11012

Genome annotation databases

GeneIDi81659
KEGGirno:81659
UCSCiRGD:621589 rat

Similar proteinsi

Entry informationi

Entry nameiGAK_RAT
AccessioniPrimary (citable) accession number: P97874
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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