Reviewed, UniProtKB/Swiss-Prot P97872 (FMO5_MOUSE)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 5
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 5
      Short name=FMO 5
    Dimethylaniline oxidase 5

Gene names

Name:

Fmo5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme By similarity.
Catalytic activity	N,N-dimethylaniline + NADPH + O(2) = N,N-dimethylaniline N-oxide + NADP(+) + H(2)O.
Cofactor	FAD.
Subcellular location	Microsome membrane. Endoplasmic reticulum membraneBy similarity.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation Methylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 533	532	Dimethylaniline monooxygenase [N-oxide-forming] 5		PRO_0000147666
Regions
	Nucleotide binding	10 – 15	6	FAD Potential
	Nucleotide binding	192 – 197	6	NADP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	5	1	Omega-N-methylated arginine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97872-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9C749652A7F823DE	FASTA	533	60,030
	10 20 30 40 50 60 MAKKRIAVIG AGASGLTCIK CCLEEGLEPV CFERSGDIGG LWRFQEAPEE GRASIYQSVV 70 80 90 100 110 120 INTSKEMMCF SDYPIPDHYP NYMHNSQVLE YFRMYAKEFD LLKYIQFKTT VCSVKKQPDF 130 140 150 160 170 180 STSGQWQVVT ECEGKQQVDV FDGVLVCTGH HTDAHLPLES FPGIEKFKGK YFHSRDYKNP 190 200 210 220 230 240 VEFTGKRVIV IGIGNSGGDL AVEISHTAKQ VFLSTRRGAW ILNRVGKHGY PIDLLLSSRI 250 260 270 280 290 300 MYYLSRICGP SLKNNYMEKQ MNQRFDHEMF GLKPKHRALS QHPTVNDDLP NRIIAGLVKV 310 320 330 340 350 360 KGNVKEFTET AAIFEDGSRE DGIDVVIFAT GYSFAFPFLE DSVKVVKNKV SLYKKVFPPN 370 380 390 400 410 420 LEKPTLAIIG LIQPLGAIMP ISELQGRWAT QVFKGLKKLP SQSEMMAEIN KAREEMAKRY 430 440 450 460 470 480 VDSQRHTIQG DYIDTMEEIA DLVGVRPNIQ PLVFTDPRLA LRLLLGPCTP VQYRLQGPGK 490 500 510 520 530 WAGARKTILT TEDRVRKPLM TRVVERDSSG GSLVTVRVLM LAVAFFAVIL AYF

References

[1]	"Molecular cloning, sequence, and expression of mouse flavin-containing monooxygenases 1 and 5 (FMO1 and FMO5)." Cherrington N.J., Falls J.G., Rose R.L., Clements K.M., Philpot R.M., Levi P.E., Hodgson E. J. Biochem. Mol. Toxicol. 12:205-212(1998) [PubMed: 9580872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90535 mRNA. Translation: AAB50013.1.
UniGene	Mm.385180
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P97872.
Genome annotation databases
Ensembl	ENSMUSG00000028088. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1310004. Fmo5.
Phylogenomic databases
HOGENOM	P97872.
HOVERGEN	P97872.
Gene expression databases
ArrayExpress	P97872.
CleanEx	MM_FMO5.
GermOnline	ENSMUSG00000028088. Mus musculus.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000960. Flavin_mOase. IPR002257. Flavin_mOase_5. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00368. FADPNR. PR00370. FMOXYGENASE. PR01125. FMOXYGENASE5.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

FMO5_MOUSE

Accession

Primary (citable) accession number: P97872

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)