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P97864

- CASP7_MOUSE

UniProt

P97864 - CASP7_MOUSE

Protein

Caspase-7

Gene

Casp7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Overexpression promotes programmed cell death By similarity.By similarity

    Catalytic activityi

    Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei144 – 1441By similarity
    Active sitei186 – 1861By similarity

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: MGI
    2. cysteine-type endopeptidase activity Source: UniProtKB
    3. cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
    4. protein binding Source: IntAct

    GO - Biological processi

    1. aging Source: Ensembl
    2. execution phase of apoptosis Source: MGI
    3. heart development Source: MGI
    4. positive regulation of neuron apoptotic process Source: MGI
    5. protein processing Source: MGI
    6. release of cytochrome c from mitochondria Source: MGI
    7. response to UV Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    BRENDAi3.4.22.60. 3474.
    ReactomeiREACT_210562. Caspase-mediated cleavage of cytoskeletal proteins.
    REACT_224460. Apoptotic cleavage of cellular proteins.
    REACT_225157. Activation of caspases through apoptosome-mediated cleavage.

    Protein family/group databases

    MEROPSiC14.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Caspase-7 (EC:3.4.22.60)
    Short name:
    CASP-7
    Alternative name(s):
    Apoptotic protease Mch-3
    Cysteine protease LICE2
    Cleaved into the following 3 chains:
    Gene namesi
    Name:Casp7
    Synonyms:Lice2, Mch3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:109383. Casp7.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. cytosol Source: Ensembl
    3. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 2323By similarityPRO_0000004624Add
    BLAST
    Initiator methioninei1 – 11Removed; alternateBy similarity
    Chaini2 – 2322Caspase-7, N-terminally processedPRO_0000424464Add
    BLAST
    Chaini24 – 198175Caspase-7 subunit p20By similarityPRO_0000004625Add
    BLAST
    Propeptidei199 – 2068By similarityPRO_0000004626
    Chaini207 – 30397Caspase-7 subunit p11By similarityPRO_0000004627Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Post-translational modificationi

    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur By similarity.By similarity

    Keywords - PTMi

    Acetylation, Zymogen

    Proteomic databases

    MaxQBiP97864.
    PaxDbiP97864.
    PRIDEiP97864.

    PTM databases

    PhosphoSiteiP97864.

    Miscellaneous databases

    PMAP-CutDBP97864.

    Expressioni

    Tissue specificityi

    Highly expressed in heart, lung, liver and kidney. Low levels in spleen, skeletal muscle and testis. No expression in the brain.

    Gene expression databases

    ArrayExpressiP97864.
    BgeeiP97864.
    CleanExiMM_CASP7.
    GenevestigatoriP97864.

    Interactioni

    Subunit structurei

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Parp1P111033EBI-5307197,EBI-642213
    Rps18P622704EBI-5307197,EBI-352460

    Protein-protein interaction databases

    BioGridi198499. 7 interactions.
    IntActiP97864. 10 interactions.
    MINTiMINT-204492.
    STRINGi10090.ENSMUSP00000026062.

    Structurei

    3D structure databases

    ProteinModelPortaliP97864.
    SMRiP97864. Positions 48-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C14A family.Curated

    Phylogenomic databases

    eggNOGiNOG279444.
    HOGENOMiHOG000231878.
    HOVERGENiHBG050802.
    InParanoidiP97864.
    KOiK04397.
    OMAiDANPRHK.
    OrthoDBiEOG7TTQ7K.
    PhylomeDBiP97864.
    TreeFamiTF102023.

    Family and domain databases

    Gene3Di3.40.50.1460. 1 hit.
    InterProiIPR029030. Caspase-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view]
    PfamiPF00656. Peptidase_C14. 1 hit.
    [Graphical view]
    PRINTSiPR00376. IL1BCENZYME.
    SMARTiSM00115. CASc. 1 hit.
    [Graphical view]
    PROSITEiPS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97864-1 [UniParc]FASTAAdd to Basket

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    MTDDQDCAAE LEKVDSSSED GVDAKPDRSS IISSILLKKK RNASAGPVRT    50
    GRDRVPTYLY RMDFQKMGKC IIINNKNFDK ATGMDVRNGT DKDAGALFKC 100
    FQNLGFEVTV HNDCSCAKMQ DLLRKASEED HSNSACFACV LLSHGEEDLI 150
    YGKDGVTPIK DLTAHFRGDR CKTLLEKPKL FFIQACRGTE LDDGIQADSG 200
    PINDIDANPR NKIPVEADFL FAYSTVPGYY SWRNPGKGSW FVQALCSILN 250
    EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP RFNEKKQIPC MVSMLTKELY 300
    FSR 303
    Length:303
    Mass (Da):34,061
    Last modified:November 1, 1997 - v2
    Checksum:i747787B5BDE5F744
    GO

    Sequence cautioni

    The sequence AAC53068.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 112EL → DW in BAA19730. (PubMed:9125129)Curated
    Sequence conflicti45 – 451A → T in BAA19730. (PubMed:9125129)Curated
    Sequence conflicti48 – 492VR → RQ in BAA19730. (PubMed:9125129)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67321 mRNA. Translation: AAC53068.1. Different initiation.
    D86353 mRNA. Translation: BAA19730.1.
    Y13088 mRNA. Translation: CAA73530.1.
    BC005428 mRNA. Translation: AAH05428.1.
    CCDSiCCDS29915.1.
    RefSeqiNP_031637.1. NM_007611.2.
    UniGeneiMm.35687.

    Genome annotation databases

    EnsembliENSMUST00000026062; ENSMUSP00000026062; ENSMUSG00000025076.
    GeneIDi12369.
    KEGGimmu:12369.
    UCSCiuc008hyw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67321 mRNA. Translation: AAC53068.1 . Different initiation.
    D86353 mRNA. Translation: BAA19730.1 .
    Y13088 mRNA. Translation: CAA73530.1 .
    BC005428 mRNA. Translation: AAH05428.1 .
    CCDSi CCDS29915.1.
    RefSeqi NP_031637.1. NM_007611.2.
    UniGenei Mm.35687.

    3D structure databases

    ProteinModelPortali P97864.
    SMRi P97864. Positions 48-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198499. 7 interactions.
    IntActi P97864. 10 interactions.
    MINTi MINT-204492.
    STRINGi 10090.ENSMUSP00000026062.

    Protein family/group databases

    MEROPSi C14.004.

    PTM databases

    PhosphoSitei P97864.

    Proteomic databases

    MaxQBi P97864.
    PaxDbi P97864.
    PRIDEi P97864.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026062 ; ENSMUSP00000026062 ; ENSMUSG00000025076 .
    GeneIDi 12369.
    KEGGi mmu:12369.
    UCSCi uc008hyw.1. mouse.

    Organism-specific databases

    CTDi 840.
    MGIi MGI:109383. Casp7.

    Phylogenomic databases

    eggNOGi NOG279444.
    HOGENOMi HOG000231878.
    HOVERGENi HBG050802.
    InParanoidi P97864.
    KOi K04397.
    OMAi DANPRHK.
    OrthoDBi EOG7TTQ7K.
    PhylomeDBi P97864.
    TreeFami TF102023.

    Enzyme and pathway databases

    BRENDAi 3.4.22.60. 3474.
    Reactomei REACT_210562. Caspase-mediated cleavage of cytoskeletal proteins.
    REACT_224460. Apoptotic cleavage of cellular proteins.
    REACT_225157. Activation of caspases through apoptosome-mediated cleavage.

    Miscellaneous databases

    ChiTaRSi CASP7. mouse.
    NextBioi 281060.
    PMAP-CutDB P97864.
    PROi P97864.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97864.
    Bgeei P97864.
    CleanExi MM_CASP7.
    Genevestigatori P97864.

    Family and domain databases

    Gene3Di 3.40.50.1460. 1 hit.
    InterProi IPR029030. Caspase-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view ]
    Pfami PF00656. Peptidase_C14. 1 hit.
    [Graphical view ]
    PRINTSi PR00376. IL1BCENZYME.
    SMARTi SM00115. CASc. 1 hit.
    [Graphical view ]
    PROSITEi PS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3."
      Juan T.S.-C., McNiece I.K., Argento J.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Fletcher F.A.
      Genomics 40:86-93(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    2. "Wortmannin enhances CPP32-like activity during neuronal differentiation of P19 embryonal carcinoma cells induced by retinoic acid."
      Mukasa T., Khoroku Y., Tsukahara T., Momoi M.Y., Kimura I., Momoi T.
      Biochem. Biophys. Res. Commun. 232:192-197(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H/An.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiCASP7_MOUSE
    AccessioniPrimary (citable) accession number: P97864
    Secondary accession number(s): O08669
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3