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P97864

- CASP7_MOUSE

UniProt

P97864 - CASP7_MOUSE

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Protein

Caspase-7

Gene

Casp7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Overexpression promotes programmed cell death (By similarity).By similarity

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei144 – 1441By similarity
Active sitei186 – 1861By similarity

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: MGI
  2. cysteine-type endopeptidase activity Source: UniProtKB
  3. cysteine-type endopeptidase activity involved in apoptotic process Source: MGI

GO - Biological processi

  1. aging Source: Ensembl
  2. execution phase of apoptosis Source: MGI
  3. heart development Source: MGI
  4. positive regulation of neuron apoptotic process Source: MGI
  5. protein processing Source: MGI
  6. release of cytochrome c from mitochondria Source: MGI
  7. response to UV Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BRENDAi3.4.22.60. 3474.
ReactomeiREACT_210562. Caspase-mediated cleavage of cytoskeletal proteins.
REACT_224460. Apoptotic cleavage of cellular proteins.
REACT_225157. Activation of caspases through apoptosome-mediated cleavage.

Protein family/group databases

MEROPSiC14.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-7 (EC:3.4.22.60)
Short name:
CASP-7
Alternative name(s):
Apoptotic protease Mch-3
Cysteine protease LICE2
Cleaved into the following 3 chains:
Gene namesi
Name:Casp7
Synonyms:Lice2, Mch3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:109383. Casp7.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. cytosol Source: Ensembl
  3. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2323By similarityPRO_0000004624Add
BLAST
Initiator methioninei1 – 11Removed; alternateBy similarity
Chaini2 – 2322Caspase-7, N-terminally processedPRO_0000424464Add
BLAST
Chaini24 – 198175Caspase-7 subunit p20By similarityPRO_0000004625Add
BLAST
Propeptidei199 – 2068By similarityPRO_0000004626
Chaini207 – 30397Caspase-7 subunit p11By similarityPRO_0000004627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Post-translational modificationi

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur (By similarity).By similarity

Keywords - PTMi

Acetylation, Zymogen

Proteomic databases

MaxQBiP97864.
PaxDbiP97864.
PRIDEiP97864.

PTM databases

PhosphoSiteiP97864.

Miscellaneous databases

PMAP-CutDBP97864.

Expressioni

Tissue specificityi

Highly expressed in heart, lung, liver and kidney. Low levels in spleen, skeletal muscle and testis. No expression in the brain.

Gene expression databases

BgeeiP97864.
CleanExiMM_CASP7.
ExpressionAtlasiP97864. baseline and differential.
GenevestigatoriP97864.

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Parp1P111033EBI-5307197,EBI-642213
Rps18P622704EBI-5307197,EBI-352460

Protein-protein interaction databases

BioGridi198499. 7 interactions.
IntActiP97864. 10 interactions.
MINTiMINT-204492.
STRINGi10090.ENSMUSP00000026062.

Structurei

3D structure databases

ProteinModelPortaliP97864.
SMRiP97864. Positions 48-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiNOG279444.
HOGENOMiHOG000231878.
HOVERGENiHBG050802.
InParanoidiP97864.
KOiK04397.
OMAiDANPRHK.
OrthoDBiEOG7TTQ7K.
PhylomeDBiP97864.
TreeFamiTF102023.

Family and domain databases

Gene3Di3.40.50.1460. 1 hit.
InterProiIPR029030. Caspase-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view]
PfamiPF00656. Peptidase_C14. 1 hit.
[Graphical view]
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00115. CASc. 1 hit.
[Graphical view]
PROSITEiPS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97864 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDDQDCAAE LEKVDSSSED GVDAKPDRSS IISSILLKKK RNASAGPVRT
60 70 80 90 100
GRDRVPTYLY RMDFQKMGKC IIINNKNFDK ATGMDVRNGT DKDAGALFKC
110 120 130 140 150
FQNLGFEVTV HNDCSCAKMQ DLLRKASEED HSNSACFACV LLSHGEEDLI
160 170 180 190 200
YGKDGVTPIK DLTAHFRGDR CKTLLEKPKL FFIQACRGTE LDDGIQADSG
210 220 230 240 250
PINDIDANPR NKIPVEADFL FAYSTVPGYY SWRNPGKGSW FVQALCSILN
260 270 280 290 300
EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP RFNEKKQIPC MVSMLTKELY

FSR
Length:303
Mass (Da):34,061
Last modified:November 1, 1997 - v2
Checksum:i747787B5BDE5F744
GO

Sequence cautioni

The sequence AAC53068.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 112EL → DW in BAA19730. (PubMed:9125129)Curated
Sequence conflicti45 – 451A → T in BAA19730. (PubMed:9125129)Curated
Sequence conflicti48 – 492VR → RQ in BAA19730. (PubMed:9125129)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U67321 mRNA. Translation: AAC53068.1. Different initiation.
D86353 mRNA. Translation: BAA19730.1.
Y13088 mRNA. Translation: CAA73530.1.
BC005428 mRNA. Translation: AAH05428.1.
CCDSiCCDS29915.1.
RefSeqiNP_031637.1. NM_007611.2.
UniGeneiMm.35687.

Genome annotation databases

EnsembliENSMUST00000026062; ENSMUSP00000026062; ENSMUSG00000025076.
GeneIDi12369.
KEGGimmu:12369.
UCSCiuc008hyw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U67321 mRNA. Translation: AAC53068.1 . Different initiation.
D86353 mRNA. Translation: BAA19730.1 .
Y13088 mRNA. Translation: CAA73530.1 .
BC005428 mRNA. Translation: AAH05428.1 .
CCDSi CCDS29915.1.
RefSeqi NP_031637.1. NM_007611.2.
UniGenei Mm.35687.

3D structure databases

ProteinModelPortali P97864.
SMRi P97864. Positions 48-303.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198499. 7 interactions.
IntActi P97864. 10 interactions.
MINTi MINT-204492.
STRINGi 10090.ENSMUSP00000026062.

Protein family/group databases

MEROPSi C14.004.

PTM databases

PhosphoSitei P97864.

Proteomic databases

MaxQBi P97864.
PaxDbi P97864.
PRIDEi P97864.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026062 ; ENSMUSP00000026062 ; ENSMUSG00000025076 .
GeneIDi 12369.
KEGGi mmu:12369.
UCSCi uc008hyw.1. mouse.

Organism-specific databases

CTDi 840.
MGIi MGI:109383. Casp7.

Phylogenomic databases

eggNOGi NOG279444.
HOGENOMi HOG000231878.
HOVERGENi HBG050802.
InParanoidi P97864.
KOi K04397.
OMAi DANPRHK.
OrthoDBi EOG7TTQ7K.
PhylomeDBi P97864.
TreeFami TF102023.

Enzyme and pathway databases

BRENDAi 3.4.22.60. 3474.
Reactomei REACT_210562. Caspase-mediated cleavage of cytoskeletal proteins.
REACT_224460. Apoptotic cleavage of cellular proteins.
REACT_225157. Activation of caspases through apoptosome-mediated cleavage.

Miscellaneous databases

ChiTaRSi CASP7. mouse.
NextBioi 281060.
PMAP-CutDB P97864.
PROi P97864.
SOURCEi Search...

Gene expression databases

Bgeei P97864.
CleanExi MM_CASP7.
ExpressionAtlasi P97864. baseline and differential.
Genevestigatori P97864.

Family and domain databases

Gene3Di 3.40.50.1460. 1 hit.
InterProi IPR029030. Caspase-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view ]
Pfami PF00656. Peptidase_C14. 1 hit.
[Graphical view ]
PRINTSi PR00376. IL1BCENZYME.
SMARTi SM00115. CASc. 1 hit.
[Graphical view ]
PROSITEi PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3."
    Juan T.S.-C., McNiece I.K., Argento J.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Fletcher F.A.
    Genomics 40:86-93(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. "Wortmannin enhances CPP32-like activity during neuronal differentiation of P19 embryonal carcinoma cells induced by retinoic acid."
    Mukasa T., Khoroku Y., Tsukahara T., Momoi M.Y., Kimura I., Momoi T.
    Biochem. Biophys. Res. Commun. 232:192-197(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/An.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCASP7_MOUSE
AccessioniPrimary (citable) accession number: P97864
Secondary accession number(s): O08669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3