Reviewed,
UniProtKB/Swiss-Prot P97864 (CASP7_MOUSE)
Last modified
November 3, 2009.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Caspase-7 Short name=CASP-7 EC=3.4.22.60 Alternative name(s): LICE2 cysteine protease Apoptotic protease Mch-3 Cleaved into the following 2 chains: 1- Recommended name: Caspase-7 subunit p20 2- Recommended name: Caspase-7 subunit p11 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 303 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Overexpression promotes programmed cell death By similarity. |
| Catalytic activity | Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-. |
| Subunit structure | Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. |
| Subcellular location | |
| Tissue specificity | Highly expressed in heart, lung, liver and kidney. Low levels in spleen, skeletal muscle and testis. No expression in the brain. |
| Post-translational modification | Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur By similarity. |
| Sequence similarities | Belongs to the peptidase C14A family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Apoptosis |
| Cellular component | Cytoplasm |
| Molecular function | Hydrolase Protease Thiol protease |
| PTM | Phosphoprotein Zymogen |
| Gene Ontology (GO) | |
| Biological process | heart development Inferred from genetic interaction. Source: MGI induction of apoptosisInferred from genetic interaction. Source: MGI release of cytochrome c from mitochondriaInferred from genetic interaction. Source: MGI response to UVInferred from genetic interaction. Source: MGI |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 23 | 23 | By similarity | PRO_0000004624 | |||||
| Chain | 24 – 198 | 175 | Caspase-7 subunit p20 By similarity | PRO_0000004625 | |||||
| Propeptide | 199 – 206 | 8 | By similarity | PRO_0000004626 | |||||
| Chain | 207 – 303 | 97 | Caspase-7 subunit p11 By similarity | PRO_0000004627 | |||||
Sites | |||||||||
| Active site | 144 | 1 | By similarity | ||||||
| Active site | 186 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 16 | 1 | Phosphoserine Ref.5 | ||||||
Experimental info | |||||||||
| Sequence conflict | 10 – 11 | 2 | EL → DW in BAA19730. Ref.2 | ||||||
| Sequence conflict | 45 | 1 | A → T in BAA19730. Ref.2 | ||||||
| Sequence conflict | 48 – 49 | 2 | VR → RQ in BAA19730. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3." Juan T.S.-C., McNiece I.K., Argento J.M., Jenkins N.A., Gilbert D.J., Copeland N.G., Fletcher F.A. Genomics 40:86-93(1997) [PubMed: 9070923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle. |
| [2] | "Wortmannin enhances CPP32-like activity during neuronal differentiation of P19 embryonal carcinoma cells induced by retinoic acid." Mukasa T., Khoroku Y., Tsukahara T., Momoi M.Y., Kimura I., Momoi T. Biochem. Biophys. Res. Commun. 232:192-197(1997) [PubMed: 9125129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Characterization of seven murine caspase family members." van de Craen M., Vandenabeele P., Declercq W., van den Brande I., van Loo G., Molemans F., Schotte P., van Criekinge W., Beyaert R., Fiers W. FEBS Lett. 403:61-69(1997) [PubMed: 9038361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/An. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U67321 mRNA. Translation: AAC53068.1. Different initiation. D86353 mRNA. Translation: BAA19730.1. Y13088 mRNA. Translation: CAA73530.1. BC005428 mRNA. Translation: AAH05428.1. | |
| IPI | IPI00130131. |
| RefSeq | NP_031637.1. |
| UniGene | Mm.35687 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1F1J based on UniProtKB P55210. |
| SMR | P97864. Positions 48-303. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P97864. |
Protein family/group databases | |
| MEROPS | C14.004. |
PTM databases | |
| PhosphoSite | P97864. |
Proteomic databases | |
| PRIDE | P97864. |
Genome annotation databases | |
| Ensembl | ENSMUST00000026062; ENSMUSP00000026062; ENSMUSG00000025076; Mus musculus. [Genome view] |
| GeneID | 12369. |
| KEGG | mmu:12369. |
| UCSC | uc008hyw.1. mouse. |
Organism-specific databases | |
| CTD | 12369. |
| MGI | MGI:109383. Casp7. |
Phylogenomic databases | |
| HOGENOM | P97864. |
| HOVERGEN | P97864. |
| OMA | NDCSCAK. |
Enzyme and pathway databases | |
| BRENDA | 3.4.22.60. 244. |
Gene expression databases | |
| ArrayExpress | P97864. |
| Bgee | P97864. |
| CleanEx | MM_CASP7. |
| Genevestigator | P97864. |
| GermOnline | ENSMUSG00000025076. Mus musculus. |
Family and domain databases | |
| InterPro | IPR015471. Casp7. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view] |
| PANTHER | PTHR10454:SF31. Casp7. 1 hit. PTHR10454. Pept_C14_p45. 1 hit. |
| Pfam | PF00656. Peptidase_C14. 1 hit. [Graphical view] |
| PRINTS | PR00376. IL1BCENZYME. |
| SMART | SM00115. CASc. 1 hit. [Graphical view] |
| PROSITE | PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 281060. |
| PMAP-CutDB | P97864. |
| SOURCE | Search... |
Entry information
| Entry name | CASP7_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P97864 Secondary accession number(s): O08669 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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