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Protein

Caspase-7

Gene

Casp7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Overexpression promotes programmed cell death (By similarity).By similarity

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei144By similarity1
Active sitei186By similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • apoptotic process Source: MGI
  • cellular response to staurosporine Source: MGI
  • execution phase of apoptosis Source: MGI
  • heart development Source: MGI
  • neuron apoptotic process Source: MGI
  • protein processing Source: MGI
  • proteolysis Source: MGI
  • response to UV Source: MGI

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processApoptosis

Enzyme and pathway databases

BRENDAi3.4.22.60. 3474.
ReactomeiR-MMU-111459. Activation of caspases through apoptosome-mediated cleavage.
R-MMU-111465. Apoptotic cleavage of cellular proteins.
R-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.

Protein family/group databases

MEROPSiC14.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-7 (EC:3.4.22.60)
Short name:
CASP-7
Alternative name(s):
Apoptotic protease Mch-3
Cysteine protease LICE2
Cleaved into the following 2 chains:
Gene namesi
Name:Casp7
Synonyms:Lice2, Mch3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:109383. Casp7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000046241 – 23By similarityAdd BLAST23
ChainiPRO_000000462524 – 198Caspase-7 subunit p20By similarityAdd BLAST175
PropeptideiPRO_0000004626199 – 206By similarity8
ChainiPRO_0000004627207 – 303Caspase-7 subunit p11By similarityAdd BLAST97

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Post-translational modificationi

Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur (By similarity).By similarity

Keywords - PTMi

Acetylation, Zymogen

Proteomic databases

EPDiP97864.
PaxDbiP97864.
PeptideAtlasiP97864.
PRIDEiP97864.

PTM databases

iPTMnetiP97864.
PhosphoSitePlusiP97864.

Miscellaneous databases

PMAP-CutDBiP97864.

Expressioni

Tissue specificityi

Highly expressed in heart, lung, liver and kidney. Low levels in spleen, skeletal muscle and testis. No expression in the brain.

Gene expression databases

BgeeiENSMUSG00000025076.
CleanExiMM_CASP7.
ExpressionAtlasiP97864. baseline and differential.
GenevisibleiP97864. MM.

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi198499. 7 interactors.
IntActiP97864. 10 interactors.
MINTiMINT-204492.
STRINGi10090.ENSMUSP00000026062.

Structurei

3D structure databases

ProteinModelPortaliP97864.
SMRiP97864.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00760000118912.
HOGENOMiHOG000231878.
HOVERGENiHBG050802.
InParanoidiP97864.
KOiK04397.
OMAiSGSWFVQ.
OrthoDBiEOG091G05YD.
PhylomeDBiP97864.
TreeFamiTF102023.

Family and domain databases

CDDicd00032. CASc. 1 hit.
InterProiView protein in InterPro
IPR029030. Caspase-like_dom_sf.
IPR033139. Caspase_cys_AS.
IPR016129. Caspase_his_AS.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
PRINTSiPR00376. IL1BCENZYME.
SMARTiView protein in SMART
SM00115. CASc. 1 hit.
SUPFAMiSSF52129. SSF52129. 1 hit.
PROSITEiView protein in PROSITE
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDDQDCAAE LEKVDSSSED GVDAKPDRSS IISSILLKKK RNASAGPVRT
60 70 80 90 100
GRDRVPTYLY RMDFQKMGKC IIINNKNFDK ATGMDVRNGT DKDAGALFKC
110 120 130 140 150
FQNLGFEVTV HNDCSCAKMQ DLLRKASEED HSNSACFACV LLSHGEEDLI
160 170 180 190 200
YGKDGVTPIK DLTAHFRGDR CKTLLEKPKL FFIQACRGTE LDDGIQADSG
210 220 230 240 250
PINDIDANPR NKIPVEADFL FAYSTVPGYY SWRNPGKGSW FVQALCSILN
260 270 280 290 300
EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP RFNEKKQIPC MVSMLTKELY

FSR
Length:303
Mass (Da):34,061
Last modified:November 1, 1997 - v2
Checksum:i747787B5BDE5F744
GO

Sequence cautioni

The sequence AAC53068 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 11EL → DW in BAA19730 (PubMed:9125129).Curated2
Sequence conflicti45A → T in BAA19730 (PubMed:9125129).Curated1
Sequence conflicti48 – 49VR → RQ in BAA19730 (PubMed:9125129).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67321 mRNA. Translation: AAC53068.1. Different initiation.
D86353 mRNA. Translation: BAA19730.1.
Y13088 mRNA. Translation: CAA73530.1.
BC005428 mRNA. Translation: AAH05428.1.
CCDSiCCDS29915.1.
RefSeqiNP_031637.1. NM_007611.2.
UniGeneiMm.35687.

Genome annotation databases

EnsembliENSMUST00000026062; ENSMUSP00000026062; ENSMUSG00000025076.
GeneIDi12369.
KEGGimmu:12369.
UCSCiuc008hyw.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCASP7_MOUSE
AccessioniPrimary (citable) accession number: P97864
Secondary accession number(s): O08669
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 22, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families