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P97863

- NFIB_MOUSE

UniProt

P97863 - NFIB_MOUSE

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Protein
Nuclear factor 1 B-type
Gene
Nfib
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi1 – 195195CTF/NF-I
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: UniProtKB
  3. RNA polymerase II transcription corepressor activity Source: Ensembl
  4. double-stranded DNA binding Source: Ensembl
  5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: UniProtKB

GO - Biological processi

  1. Clara cell differentiation Source: UniProtKB
  2. DNA replication Source: UniProtKB-KW
  3. Type I pneumocyte differentiation Source: UniProtKB
  4. Type II pneumocyte differentiation Source: UniProtKB
  5. anterior commissure morphogenesis Source: UniProtKB
  6. chondrocyte differentiation Source: UniProtKB
  7. commissural neuron axon guidance Source: UniProtKB
  8. forebrain development Source: MGI
  9. glial cell differentiation Source: UniProtKB
  10. hindbrain development Source: MGI
  11. lung ciliated cell differentiation Source: UniProtKB
  12. lung development Source: MGI
  13. negative regulation of DNA binding Source: Ensembl
  14. negative regulation of cell proliferation Source: MGI
  15. negative regulation of epithelial cell proliferation involved in lung morphogenesis Source: UniProtKB
  16. negative regulation of mesenchymal cell proliferation involved in lung development Source: UniProtKB
  17. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  18. principal sensory nucleus of trigeminal nerve development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_221403. RNA Polymerase III Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 B-type
Short name:
NF1-B
Short name:
Nuclear factor 1/B
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/B
Short name:
NF-I/B
Short name:
NFI-B
TGGCA-binding protein
Gene namesi
Name:Nfib
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:103188. Nfib.

Subcellular locationi

GO - Cellular componenti

  1. cerebellar mossy fiber Source: UniProtKB
  2. nucleolus Source: Ensembl
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Nuclear factor 1 B-type
PRO_0000100197Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei264 – 2641Phosphoserine By similarity
Modified residuei292 – 2921Phosphoserine1 Publication
Modified residuei295 – 2951Phosphoserine2 Publications
Modified residuei312 – 3121Phosphoserine By similarity
Modified residuei328 – 3281Phosphoserine By similarity
Modified residuei333 – 3331Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97863.
PaxDbiP97863.
PRIDEiP97863.

PTM databases

PhosphoSiteiP97863.

Expressioni

Tissue specificityi

Highest expression in lung, skeletal muscle and heart. Lower levels in liver, kidney and brain. Very low levels in testis and spleen.

Gene expression databases

ArrayExpressiP97863.
BgeeiP97863.
CleanExiMM_NFIB.
GenevestigatoriP97863.

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

IntActiP97863. 2 interactions.
MINTiMINT-4103630.

Structurei

3D structure databases

ProteinModelPortaliP97863.

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.

Phylogenomic databases

eggNOGiNOG252172.
GeneTreeiENSGT00390000009905.
HOGENOMiHOG000013028.
HOVERGENiHBG006561.
InParanoidiP97863.
KOiK09169.
OMAiYYVQDQD.
PhylomeDBiP97863.
TreeFamiTF313889.

Family and domain databases

InterProiIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERiPTHR11492. PTHR11492. 1 hit.
PfamiPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
[Graphical view]
PROSITEiPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97863-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS    50
KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK 100
HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER 150
LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHSDP 200
AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY 250
HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS 300
RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV 350
AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK 400
NYVPSYDPSS PQTSQPNSSG QVVGKVPGHF TPVLAPSPHP SAVRPVTLTM 450
TDTKPITTST EGEAASPTAT TYTASGTSQA NRYVGLSPRD PSFLHQQQLR 500
ICDWTMNQNG RHLYPSTSED TLGITWQSPG TWASLVPFQV SNRTPILPAN 550
VQNYGLNIIG EPFLQAETSN 570
Length:570
Mass (Da):63,507
Last modified:June 1, 2001 - v2
Checksum:iD94AB4400A2811CC
GO
Isoform 2 (identifier: P97863-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-498: Missing.

Show »
Length:487
Mass (Da):54,908
Checksum:i868043916A36234F
GO
Isoform 3 (identifier: P97863-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-420: PNSSG → SWYLG
     421-570: Missing.

Show »
Length:420
Mass (Da):47,472
Checksum:iE26259E1F965146D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti131 – 1311D → G in strain: NIH Swiss.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei416 – 49883Missing in isoform 2.
VSP_003547Add
BLAST
Alternative sequencei416 – 4205PNSSG → SWYLG in isoform 3.
VSP_003548
Alternative sequencei421 – 570150Missing in isoform 3.
VSP_003549Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U57634 mRNA. Translation: AAB49929.1.
Y07685 mRNA. Translation: CAA68949.1.
Y07686 mRNA. Translation: CAA68950.1.
Y07687 mRNA. Translation: CAA68951.1.
BC014290 mRNA. Translation: AAH14290.1.
AF111264 Genomic DNA. Translation: AAD39099.1.
CCDSiCCDS38790.1. [P97863-3]
CCDS51213.1. [P97863-1]
RefSeqiNP_001106680.1. NM_001113209.2. [P97863-1]
NP_032713.3. NM_008687.6. [P97863-3]
UniGeneiMm.317947.

Genome annotation databases

EnsembliENSMUST00000050872; ENSMUSP00000052863; ENSMUSG00000008575. [P97863-1]
ENSMUST00000064770; ENSMUSP00000067629; ENSMUSG00000008575. [P97863-3]
GeneIDi18028.
KEGGimmu:18028.
UCSCiuc008tjz.2. mouse. [P97863-1]
uc008tkd.2. mouse. [P97863-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U57634 mRNA. Translation: AAB49929.1 .
Y07685 mRNA. Translation: CAA68949.1 .
Y07686 mRNA. Translation: CAA68950.1 .
Y07687 mRNA. Translation: CAA68951.1 .
BC014290 mRNA. Translation: AAH14290.1 .
AF111264 Genomic DNA. Translation: AAD39099.1 .
CCDSi CCDS38790.1. [P97863-3 ]
CCDS51213.1. [P97863-1 ]
RefSeqi NP_001106680.1. NM_001113209.2. [P97863-1 ]
NP_032713.3. NM_008687.6. [P97863-3 ]
UniGenei Mm.317947.

3D structure databases

ProteinModelPortali P97863.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97863. 2 interactions.
MINTi MINT-4103630.

PTM databases

PhosphoSitei P97863.

Proteomic databases

MaxQBi P97863.
PaxDbi P97863.
PRIDEi P97863.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050872 ; ENSMUSP00000052863 ; ENSMUSG00000008575 . [P97863-1 ]
ENSMUST00000064770 ; ENSMUSP00000067629 ; ENSMUSG00000008575 . [P97863-3 ]
GeneIDi 18028.
KEGGi mmu:18028.
UCSCi uc008tjz.2. mouse. [P97863-1 ]
uc008tkd.2. mouse. [P97863-2 ]

Organism-specific databases

CTDi 4781.
MGIi MGI:103188. Nfib.

Phylogenomic databases

eggNOGi NOG252172.
GeneTreei ENSGT00390000009905.
HOGENOMi HOG000013028.
HOVERGENi HBG006561.
InParanoidi P97863.
KOi K09169.
OMAi YYVQDQD.
PhylomeDBi P97863.
TreeFami TF313889.

Enzyme and pathway databases

Reactomei REACT_221403. RNA Polymerase III Transcription Termination.

Miscellaneous databases

ChiTaRSi NFIB. mouse.
NextBioi 293099.
PROi P97863.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97863.
Bgeei P97863.
CleanExi MM_NFIB.
Genevestigatori P97863.

Family and domain databases

InterProi IPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view ]
PANTHERi PTHR11492. PTHR11492. 1 hit.
Pfami PF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view ]
SMARTi SM00523. DWA. 1 hit.
[Graphical view ]
PROSITEi PS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
    Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
    Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: BALB/c.
    Tissue: Liver and Skeletal muscle.
  2. "Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
    Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
    Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3).
    Strain: NIH Swiss.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
    Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
    Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-187.
    Strain: 129.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNFIB_MOUSE
AccessioniPrimary (citable) accession number: P97863
Secondary accession number(s): P70252
, P70253, P70254, Q9R1G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi