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P97863

- NFIB_MOUSE

UniProt

P97863 - NFIB_MOUSE

Protein

Nuclear factor 1 B-type

Gene

Nfib

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi1 – 195195CTF/NF-IPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB
    2. double-stranded DNA binding Source: Ensembl
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: UniProtKB
    4. RNA polymerase II transcription corepressor activity Source: Ensembl
    5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. anterior commissure morphogenesis Source: UniProtKB
    2. chondrocyte differentiation Source: UniProtKB
    3. Clara cell differentiation Source: UniProtKB
    4. commissural neuron axon guidance Source: UniProtKB
    5. DNA replication Source: UniProtKB-KW
    6. forebrain development Source: MGI
    7. glial cell differentiation Source: UniProtKB
    8. hindbrain development Source: MGI
    9. lung ciliated cell differentiation Source: UniProtKB
    10. lung development Source: MGI
    11. negative regulation of cell proliferation Source: MGI
    12. negative regulation of DNA binding Source: Ensembl
    13. negative regulation of epithelial cell proliferation involved in lung morphogenesis Source: UniProtKB
    14. negative regulation of mesenchymal cell proliferation involved in lung development Source: UniProtKB
    15. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    16. principal sensory nucleus of trigeminal nerve development Source: UniProtKB
    17. Type II pneumocyte differentiation Source: UniProtKB
    18. Type I pneumocyte differentiation Source: UniProtKB

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    DNA replication, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_221403. RNA Polymerase III Transcription Termination.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear factor 1 B-type
    Short name:
    NF1-B
    Short name:
    Nuclear factor 1/B
    Alternative name(s):
    CCAAT-box-binding transcription factor
    Short name:
    CTF
    Nuclear factor I/B
    Short name:
    NF-I/B
    Short name:
    NFI-B
    TGGCA-binding protein
    Gene namesi
    Name:Nfib
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:103188. Nfib.

    Subcellular locationi

    GO - Cellular componenti

    1. cerebellar mossy fiber Source: UniProtKB
    2. nucleolus Source: Ensembl
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 570570Nuclear factor 1 B-typePRO_0000100197Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei264 – 2641PhosphoserineBy similarity
    Modified residuei292 – 2921Phosphoserine1 Publication
    Modified residuei295 – 2951Phosphoserine2 Publications
    Modified residuei312 – 3121PhosphoserineBy similarity
    Modified residuei328 – 3281PhosphoserineBy similarity
    Modified residuei333 – 3331PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP97863.
    PaxDbiP97863.
    PRIDEiP97863.

    PTM databases

    PhosphoSiteiP97863.

    Expressioni

    Tissue specificityi

    Highest expression in lung, skeletal muscle and heart. Lower levels in liver, kidney and brain. Very low levels in testis and spleen.

    Gene expression databases

    ArrayExpressiP97863.
    BgeeiP97863.
    CleanExiMM_NFIB.
    GenevestigatoriP97863.

    Interactioni

    Subunit structurei

    Binds DNA as a homodimer.

    Protein-protein interaction databases

    IntActiP97863. 2 interactions.
    MINTiMINT-4103630.

    Structurei

    3D structure databases

    ProteinModelPortaliP97863.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CTF/NF-I family.PROSITE-ProRule annotation
    Contains 1 CTF/NF-I DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG252172.
    GeneTreeiENSGT00390000009905.
    HOGENOMiHOG000013028.
    HOVERGENiHBG006561.
    InParanoidiP97863.
    KOiK09169.
    OMAiYYVQDQD.
    PhylomeDBiP97863.
    TreeFamiTF313889.

    Family and domain databases

    InterProiIPR000647. CTF/NFI.
    IPR020604. CTF/NFI_DNA-bd-dom.
    IPR019739. CTF/NFI_DNA-bd_CS.
    IPR019548. CTF/NFI_DNA-bd_N.
    IPR003619. MAD_homology1_Dwarfin-type.
    [Graphical view]
    PANTHERiPTHR11492. PTHR11492. 1 hit.
    PfamiPF00859. CTF_NFI. 1 hit.
    PF03165. MH1. 1 hit.
    PF10524. NfI_DNAbd_pre-N. 1 hit.
    [Graphical view]
    SMARTiSM00523. DWA. 1 hit.
    [Graphical view]
    PROSITEiPS00349. CTF_NFI_1. 1 hit.
    PS51080. CTF_NFI_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P97863-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS    50
    KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK 100
    HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER 150
    LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHSDP 200
    AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY 250
    HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS 300
    RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV 350
    AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK 400
    NYVPSYDPSS PQTSQPNSSG QVVGKVPGHF TPVLAPSPHP SAVRPVTLTM 450
    TDTKPITTST EGEAASPTAT TYTASGTSQA NRYVGLSPRD PSFLHQQQLR 500
    ICDWTMNQNG RHLYPSTSED TLGITWQSPG TWASLVPFQV SNRTPILPAN 550
    VQNYGLNIIG EPFLQAETSN 570
    Length:570
    Mass (Da):63,507
    Last modified:June 1, 2001 - v2
    Checksum:iD94AB4400A2811CC
    GO
    Isoform 2 (identifier: P97863-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         416-498: Missing.

    Show »
    Length:487
    Mass (Da):54,908
    Checksum:i868043916A36234F
    GO
    Isoform 3 (identifier: P97863-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         416-420: PNSSG → SWYLG
         421-570: Missing.

    Show »
    Length:420
    Mass (Da):47,472
    Checksum:iE26259E1F965146D
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti131 – 1311D → G in strain: NIH Swiss.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei416 – 49883Missing in isoform 2. CuratedVSP_003547Add
    BLAST
    Alternative sequencei416 – 4205PNSSG → SWYLG in isoform 3. 1 PublicationVSP_003548
    Alternative sequencei421 – 570150Missing in isoform 3. 1 PublicationVSP_003549Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57634 mRNA. Translation: AAB49929.1.
    Y07685 mRNA. Translation: CAA68949.1.
    Y07686 mRNA. Translation: CAA68950.1.
    Y07687 mRNA. Translation: CAA68951.1.
    BC014290 mRNA. Translation: AAH14290.1.
    AF111264 Genomic DNA. Translation: AAD39099.1.
    CCDSiCCDS38790.1. [P97863-3]
    CCDS51213.1. [P97863-1]
    RefSeqiNP_001106680.1. NM_001113209.2. [P97863-1]
    NP_032713.3. NM_008687.6. [P97863-3]
    UniGeneiMm.317947.

    Genome annotation databases

    EnsembliENSMUST00000050872; ENSMUSP00000052863; ENSMUSG00000008575. [P97863-1]
    ENSMUST00000064770; ENSMUSP00000067629; ENSMUSG00000008575. [P97863-3]
    GeneIDi18028.
    KEGGimmu:18028.
    UCSCiuc008tjz.2. mouse. [P97863-1]
    uc008tkd.2. mouse. [P97863-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57634 mRNA. Translation: AAB49929.1 .
    Y07685 mRNA. Translation: CAA68949.1 .
    Y07686 mRNA. Translation: CAA68950.1 .
    Y07687 mRNA. Translation: CAA68951.1 .
    BC014290 mRNA. Translation: AAH14290.1 .
    AF111264 Genomic DNA. Translation: AAD39099.1 .
    CCDSi CCDS38790.1. [P97863-3 ]
    CCDS51213.1. [P97863-1 ]
    RefSeqi NP_001106680.1. NM_001113209.2. [P97863-1 ]
    NP_032713.3. NM_008687.6. [P97863-3 ]
    UniGenei Mm.317947.

    3D structure databases

    ProteinModelPortali P97863.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97863. 2 interactions.
    MINTi MINT-4103630.

    PTM databases

    PhosphoSitei P97863.

    Proteomic databases

    MaxQBi P97863.
    PaxDbi P97863.
    PRIDEi P97863.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050872 ; ENSMUSP00000052863 ; ENSMUSG00000008575 . [P97863-1 ]
    ENSMUST00000064770 ; ENSMUSP00000067629 ; ENSMUSG00000008575 . [P97863-3 ]
    GeneIDi 18028.
    KEGGi mmu:18028.
    UCSCi uc008tjz.2. mouse. [P97863-1 ]
    uc008tkd.2. mouse. [P97863-2 ]

    Organism-specific databases

    CTDi 4781.
    MGIi MGI:103188. Nfib.

    Phylogenomic databases

    eggNOGi NOG252172.
    GeneTreei ENSGT00390000009905.
    HOGENOMi HOG000013028.
    HOVERGENi HBG006561.
    InParanoidi P97863.
    KOi K09169.
    OMAi YYVQDQD.
    PhylomeDBi P97863.
    TreeFami TF313889.

    Enzyme and pathway databases

    Reactomei REACT_221403. RNA Polymerase III Transcription Termination.

    Miscellaneous databases

    ChiTaRSi NFIB. mouse.
    NextBioi 293099.
    PROi P97863.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97863.
    Bgeei P97863.
    CleanExi MM_NFIB.
    Genevestigatori P97863.

    Family and domain databases

    InterProi IPR000647. CTF/NFI.
    IPR020604. CTF/NFI_DNA-bd-dom.
    IPR019739. CTF/NFI_DNA-bd_CS.
    IPR019548. CTF/NFI_DNA-bd_N.
    IPR003619. MAD_homology1_Dwarfin-type.
    [Graphical view ]
    PANTHERi PTHR11492. PTHR11492. 1 hit.
    Pfami PF00859. CTF_NFI. 1 hit.
    PF03165. MH1. 1 hit.
    PF10524. NfI_DNAbd_pre-N. 1 hit.
    [Graphical view ]
    SMARTi SM00523. DWA. 1 hit.
    [Graphical view ]
    PROSITEi PS00349. CTF_NFI_1. 1 hit.
    PS51080. CTF_NFI_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
      Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
      Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
      Strain: BALB/c.
      Tissue: Liver and Skeletal muscle.
    2. "Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
      Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
      Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3).
      Strain: NIH Swiss.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
      Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
      Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-187.
      Strain: 129.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiNFIB_MOUSE
    AccessioniPrimary (citable) accession number: P97863
    Secondary accession number(s): P70252
    , P70253, P70254, Q9R1G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3