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P97863

- NFIB_MOUSE

UniProt

P97863 - NFIB_MOUSE

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Protein

Nuclear factor 1 B-type

Gene

Nfib

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi1 – 195195CTF/NF-IPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. double-stranded DNA binding Source: Ensembl
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: UniProtKB
  5. RNA polymerase II transcription corepressor activity Source: Ensembl
  6. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: UniProtKB

GO - Biological processi

  1. anterior commissure morphogenesis Source: UniProtKB
  2. chondrocyte differentiation Source: UniProtKB
  3. Clara cell differentiation Source: UniProtKB
  4. commissural neuron axon guidance Source: UniProtKB
  5. DNA replication Source: UniProtKB-KW
  6. forebrain development Source: MGI
  7. glial cell differentiation Source: UniProtKB
  8. hindbrain development Source: MGI
  9. lung ciliated cell differentiation Source: UniProtKB
  10. lung development Source: MGI
  11. negative regulation of cell proliferation Source: MGI
  12. negative regulation of DNA binding Source: Ensembl
  13. negative regulation of epithelial cell proliferation involved in lung morphogenesis Source: UniProtKB
  14. negative regulation of mesenchymal cell proliferation involved in lung development Source: UniProtKB
  15. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  16. principal sensory nucleus of trigeminal nerve development Source: UniProtKB
  17. Type II pneumocyte differentiation Source: UniProtKB
  18. Type I pneumocyte differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_221403. RNA Polymerase III Transcription Termination.
REACT_261233. RNA Polymerase III Abortive And Retractive Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 B-type
Short name:
NF1-B
Short name:
Nuclear factor 1/B
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/B
Short name:
NF-I/B
Short name:
NFI-B
TGGCA-binding protein
Gene namesi
Name:Nfib
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:103188. Nfib.

Subcellular locationi

GO - Cellular componenti

  1. cerebellar mossy fiber Source: UniProtKB
  2. nucleolus Source: Ensembl
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Nuclear factor 1 B-typePRO_0000100197Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei264 – 2641PhosphoserineBy similarity
Modified residuei292 – 2921Phosphoserine1 Publication
Modified residuei295 – 2951Phosphoserine2 Publications
Modified residuei312 – 3121PhosphoserineBy similarity
Modified residuei328 – 3281PhosphoserineBy similarity
Modified residuei333 – 3331PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97863.
PaxDbiP97863.
PRIDEiP97863.

PTM databases

PhosphoSiteiP97863.

Expressioni

Tissue specificityi

Highest expression in lung, skeletal muscle and heart. Lower levels in liver, kidney and brain. Very low levels in testis and spleen.

Gene expression databases

BgeeiP97863.
CleanExiMM_NFIB.
ExpressionAtlasiP97863. baseline and differential.
GenevestigatoriP97863.

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

IntActiP97863. 2 interactions.
MINTiMINT-4103630.

Structurei

3D structure databases

ProteinModelPortaliP97863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.PROSITE-ProRule annotation
Contains 1 CTF/NF-I DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG252172.
GeneTreeiENSGT00390000009905.
HOGENOMiHOG000013028.
HOVERGENiHBG006561.
InParanoidiP97863.
KOiK09169.
OMAiYYVQDQD.
PhylomeDBiP97863.
TreeFamiTF313889.

Family and domain databases

InterProiIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERiPTHR11492. PTHR11492. 1 hit.
PfamiPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
[Graphical view]
PROSITEiPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97863-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS
60 70 80 90 100
KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK
110 120 130 140 150
HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER
160 170 180 190 200
LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHSDP
210 220 230 240 250
AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY
260 270 280 290 300
HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS
310 320 330 340 350
RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV
360 370 380 390 400
AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK
410 420 430 440 450
NYVPSYDPSS PQTSQPNSSG QVVGKVPGHF TPVLAPSPHP SAVRPVTLTM
460 470 480 490 500
TDTKPITTST EGEAASPTAT TYTASGTSQA NRYVGLSPRD PSFLHQQQLR
510 520 530 540 550
ICDWTMNQNG RHLYPSTSED TLGITWQSPG TWASLVPFQV SNRTPILPAN
560 570
VQNYGLNIIG EPFLQAETSN
Length:570
Mass (Da):63,507
Last modified:June 1, 2001 - v2
Checksum:iD94AB4400A2811CC
GO
Isoform 2 (identifier: P97863-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-498: Missing.

Show »
Length:487
Mass (Da):54,908
Checksum:i868043916A36234F
GO
Isoform 3 (identifier: P97863-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-420: PNSSG → SWYLG
     421-570: Missing.

Show »
Length:420
Mass (Da):47,472
Checksum:iE26259E1F965146D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti131 – 1311D → G in strain: NIH Swiss.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei416 – 49883Missing in isoform 2. CuratedVSP_003547Add
BLAST
Alternative sequencei416 – 4205PNSSG → SWYLG in isoform 3. 1 PublicationVSP_003548
Alternative sequencei421 – 570150Missing in isoform 3. 1 PublicationVSP_003549Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57634 mRNA. Translation: AAB49929.1.
Y07685 mRNA. Translation: CAA68949.1.
Y07686 mRNA. Translation: CAA68950.1.
Y07687 mRNA. Translation: CAA68951.1.
BC014290 mRNA. Translation: AAH14290.1.
AF111264 Genomic DNA. Translation: AAD39099.1.
CCDSiCCDS38790.1. [P97863-3]
CCDS51213.1. [P97863-1]
RefSeqiNP_001106680.1. NM_001113209.2. [P97863-1]
NP_032713.3. NM_008687.6. [P97863-3]
UniGeneiMm.317947.

Genome annotation databases

EnsembliENSMUST00000050872; ENSMUSP00000052863; ENSMUSG00000008575. [P97863-1]
ENSMUST00000064770; ENSMUSP00000067629; ENSMUSG00000008575. [P97863-3]
GeneIDi18028.
KEGGimmu:18028.
UCSCiuc008tjz.2. mouse. [P97863-1]
uc008tkd.2. mouse. [P97863-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57634 mRNA. Translation: AAB49929.1 .
Y07685 mRNA. Translation: CAA68949.1 .
Y07686 mRNA. Translation: CAA68950.1 .
Y07687 mRNA. Translation: CAA68951.1 .
BC014290 mRNA. Translation: AAH14290.1 .
AF111264 Genomic DNA. Translation: AAD39099.1 .
CCDSi CCDS38790.1. [P97863-3 ]
CCDS51213.1. [P97863-1 ]
RefSeqi NP_001106680.1. NM_001113209.2. [P97863-1 ]
NP_032713.3. NM_008687.6. [P97863-3 ]
UniGenei Mm.317947.

3D structure databases

ProteinModelPortali P97863.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97863. 2 interactions.
MINTi MINT-4103630.

PTM databases

PhosphoSitei P97863.

Proteomic databases

MaxQBi P97863.
PaxDbi P97863.
PRIDEi P97863.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050872 ; ENSMUSP00000052863 ; ENSMUSG00000008575 . [P97863-1 ]
ENSMUST00000064770 ; ENSMUSP00000067629 ; ENSMUSG00000008575 . [P97863-3 ]
GeneIDi 18028.
KEGGi mmu:18028.
UCSCi uc008tjz.2. mouse. [P97863-1 ]
uc008tkd.2. mouse. [P97863-2 ]

Organism-specific databases

CTDi 4781.
MGIi MGI:103188. Nfib.

Phylogenomic databases

eggNOGi NOG252172.
GeneTreei ENSGT00390000009905.
HOGENOMi HOG000013028.
HOVERGENi HBG006561.
InParanoidi P97863.
KOi K09169.
OMAi YYVQDQD.
PhylomeDBi P97863.
TreeFami TF313889.

Enzyme and pathway databases

Reactomei REACT_221403. RNA Polymerase III Transcription Termination.
REACT_261233. RNA Polymerase III Abortive And Retractive Initiation.

Miscellaneous databases

ChiTaRSi Nfib. mouse.
NextBioi 293099.
PROi P97863.
SOURCEi Search...

Gene expression databases

Bgeei P97863.
CleanExi MM_NFIB.
ExpressionAtlasi P97863. baseline and differential.
Genevestigatori P97863.

Family and domain databases

InterProi IPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view ]
PANTHERi PTHR11492. PTHR11492. 1 hit.
Pfami PF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view ]
SMARTi SM00523. DWA. 1 hit.
[Graphical view ]
PROSITEi PS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
    Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
    Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: BALB/c.
    Tissue: Liver and Skeletal muscle.
  2. "Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
    Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
    Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3).
    Strain: NIH Swiss.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
    Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
    Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-187.
    Strain: 129.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNFIB_MOUSE
AccessioniPrimary (citable) accession number: P97863
Secondary accession number(s): P70252
, P70253, P70254, Q9R1G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3