Nuclear factor 1 B-type - Mus musculus (Mouse)

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P97863 (NFIB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nuclear factor 1 B-type
Short name=NF1-B
Short name=Nuclear factor 1/B

Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CTF
Nuclear factor I/B
Short name=NF-I/B
Short name=NFI-B
TGGCA-binding protein

Gene names

Name:

Nfib

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Subunit structure	Binds DNA as a homodimer.
Subcellular location	Nucleus.
Tissue specificity	Highest expression in lung, skeletal muscle and heart. Lower levels in liver, kidney and brain. Very low levels in testis and spleen.
Sequence similarities	Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.

Ontologies

Keywords
Biological process	DNA replication Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Clara cell differentiation Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB DNA replication Inferred from electronic annotation. Source: UniProtKB-KW Type I pneumocyte differentiation Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB Type II pneumocyte differentiation Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB anterior commissure morphogenesis Inferred from mutant phenotype PubMed 19961580. Source: UniProtKB chondrocyte differentiation Inferred from mutant phenotype PubMed 17904922. Source: UniProtKB commissural neuron axon guidance Inferred from mutant phenotype PubMed 19961580. Source: UniProtKB glial cell differentiation Inferred from mutant phenotype PubMed 19961580. Source: UniProtKB hindbrain development Inferred from mutant phenotype PubMed 15632069. Source: MGI lung ciliated cell differentiation Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB negative regulation of epithelial cell proliferation involved in lung morphogenesis Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB negative regulation of mesenchymal cell proliferation involved in lung development Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 19706729. Source: UniProtKB principal sensory nucleus of trigeminal nerve development Inferred from mutant phenotype PubMed 19107796. Source: UniProtKB
Cellular_component	cerebellar mossy fiber Inferred from direct assay PubMed 19107796. Source: UniProtKB nucleolus Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 21513708. Source: UniProtKB
Molecular_function	DNA binding Inferred from sequence or structural similarity. Source: UniProtKB RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay PubMed 21513708. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P97863-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P97863-2) The sequence of this isoform differs from the canonical sequence as follows: 416-498: Missing.

Isoform 3 (identifier: P97863-3) The sequence of this isoform differs from the canonical sequence as follows: 416-420: PNSSG → SWYLG 421-570: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 570

569

Nuclear factor 1 B-type

PRO_0000100197

Regions

DNA binding

1 – 195

195

CTF/NF-I

Amino acid modifications

Modified residue

N-acetylmethionine By similarity

Modified residue

Phosphoserine Ref.6

Modified residue

264

Phosphoserine Ref.7

Modified residue

292

Phosphoserine Ref.7

Modified residue

295

Phosphoserine Ref.5 Ref.7

Modified residue

312

Phosphoserine By similarity

Modified residue

328

Phosphoserine By similarity

Modified residue

333

Phosphoserine By similarity

Natural variations

Alternative sequence

416 – 498

Missing in isoform 2.

VSP_003547

Alternative sequence

416 – 420

PNSSG → SWYLG in isoform 3.

VSP_003548

Alternative sequence

421 – 570

150

Missing in isoform 3.

VSP_003549

Natural variant

131

D → G in strain: NIH Swiss.

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: D94AB4400A2811CC Show »	FASTA	570	63,507
10 20 30 40 50 60 MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 70 80 90 100 110 120 LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC 130 140 150 160 170 180 LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL 190 200 210 220 230 240 AYYVQEQDSG QSGSPSHSDP AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP 250 260 270 280 290 300 IGEIPSQPYY HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS 310 320 330 340 350 360 RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP 370 380 390 400 410 420 PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQPNSSG 430 440 450 460 470 480 QVVGKVPGHF TPVLAPSPHP SAVRPVTLTM TDTKPITTST EGEAASPTAT TYTASGTSQA 490 500 510 520 530 540 NRYVGLSPRD PSFLHQQQLR ICDWTMNQNG RHLYPSTSED TLGITWQSPG TWASLVPFQV 550 560 570 SNRTPILPAN VQNYGLNIIG EPFLQAETSN « Hide
Isoform 2 [UniParc]. Checksum: 868043916A36234F Show »	FASTA	487	54,908
10 20 30 40 50 60 MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 70 80 90 100 110 120 LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC 130 140 150 160 170 180 LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL 190 200 210 220 230 240 AYYVQEQDSG QSGSPSHSDP AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP 250 260 270 280 290 300 IGEIPSQPYY HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS 310 320 330 340 350 360 RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP 370 380 390 400 410 420 PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQLRICD 430 440 450 460 470 480 WTMNQNGRHL YPSTSEDTLG ITWQSPGTWA SLVPFQVSNR TPILPANVQN YGLNIIGEPF LQAETSN « Hide
Isoform 3 [UniParc]. Checksum: E26259E1F965146D Show »	FASTA	420	47,472
10 20 30 40 50 60 MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 70 80 90 100 110 120 LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC 130 140 150 160 170 180 LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL 190 200 210 220 230 240 AYYVQEQDSG QSGSPSHSDP AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP 250 260 270 280 290 300 IGEIPSQPYY HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS 310 320 330 340 350 360 RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP 370 380 390 400 410 420 PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQSWYLG « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development." Chaudhry A.Z., Lyons G.E., Gronostajski R.M. Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Strain: BALB/c. Tissue: Liver and Skeletal muscle.
[2]	"Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One." Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E. Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3). Strain: NIH Swiss.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals." Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M. Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-187. Strain: 129.
[5]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295, MASS SPECTROMETRY. Tissue: Embryonic brain.
[6]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264; SER-292 AND SER-295, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57634 mRNA. Translation: AAB49929.1. Y07685 mRNA. Translation: CAA68949.1. Y07686 mRNA. Translation: CAA68950.1. Y07687 mRNA. Translation: CAA68951.1. BC014290 mRNA. Translation: AAH14290.1. AF111264 Genomic DNA. Translation: AAD39099.1.
IPI	IPI00130129. IPI00230656. IPI00750303.
RefSeq	NP_001106680.1. NM_001113209.1. NP_032713.3. NM_008687.5.
UniGene	Mm.317947.
3D structure databases
ProteinModelPortal	P97863.
ModBase	Search...
Protein-protein interaction databases
IntAct	P97863. 1 interaction.
PTM databases
PhosphoSite	P97863.
Proteomic databases
PaxDb	P97863.
PRIDE	P97863.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050872; ENSMUSP00000052863; ENSMUSG00000008575. ENSMUST00000064770; ENSMUSP00000067629; ENSMUSG00000008575.
GeneID	18028.
KEGG	mmu:18028.
UCSC	uc008tjz.2. mouse. uc008tkd.2. mouse.
Organism-specific databases
CTD	4781.
MGI	MGI:103188. Nfib.
Phylogenomic databases
eggNOG	NOG252172.
GeneTree	ENSGT00390000009905.
HOGENOM	HOG000013028.
HOVERGEN	HBG006561.
InParanoid	P97863.
KO	K09169.
OMA	YYVQDQD.
Gene expression databases
ArrayExpress	P97863.
Bgee	P97863.
CleanEx	MM_NFIB.
Genevestigator	P97863.
GermOnline	ENSMUSG00000008575. Mus musculus.
Family and domain databases
InterPro	IPR000647. CTF/NFI. IPR020604. CTF/NFI_DNA-bd-dom. IPR019739. CTF/NFI_DNA-bd_CS. IPR019548. CTF/NFI_DNA-bd_N. IPR003619. MAD_homology1_Dwarfin-type. [Graphical view]
PANTHER	PTHR11492. PTHR11492. 1 hit.
Pfam	PF00859. CTF_NFI. 1 hit. PF03165. MH1. 1 hit. PF10524. NfI_DNAbd_pre-N. 1 hit. [Graphical view]
SMART	SM00523. DWA. 1 hit. [Graphical view]
PROSITE	PS00349. CTF_NFI_1. 1 hit. PS51080. CTF_NFI_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NFIB. mouse.
NextBio	293099.
SOURCE	Search...

Entry information

Entry name

NFIB_MOUSE

Accession

Primary (citable) accession number: P97863
Secondary accession number(s): P70252

Q9R1G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents