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P97863 (NFIB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear factor 1 B-type

Short name=NF1-B
Short name=Nuclear factor 1/B
Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CTF
Nuclear factor I/B
Short name=NF-I/B
Short name=NFI-B
TGGCA-binding protein
Gene names
Name:Nfib
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Subunit structure

Binds DNA as a homodimer.

Subcellular location

Nucleus.

Tissue specificity

Highest expression in lung, skeletal muscle and heart. Lower levels in liver, kidney and brain. Very low levels in testis and spleen.

Sequence similarities

Belongs to the CTF/NF-I family.

Contains 1 CTF/NF-I DNA-binding domain.

Ontologies

Keywords
   Biological processDNA replication
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processClara cell differentiation

Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

Type I pneumocyte differentiation

Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB

Type II pneumocyte differentiation

Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB

anterior commissure morphogenesis

Inferred from mutant phenotype PubMed 19961580. Source: UniProtKB

chondrocyte differentiation

Inferred from mutant phenotype PubMed 17904922. Source: UniProtKB

commissural neuron axon guidance

Inferred from mutant phenotype PubMed 19961580. Source: UniProtKB

forebrain development

Inferred from mutant phenotype PubMed 15632069. Source: MGI

glial cell differentiation

Inferred from mutant phenotype PubMed 19961580. Source: UniProtKB

hindbrain development

Inferred from mutant phenotype PubMed 15632069. Source: MGI

lung ciliated cell differentiation

Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB

lung development

Inferred from mutant phenotype PubMed 11850179PubMed 15632069. Source: MGI

negative regulation of DNA binding

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 15632069. Source: MGI

negative regulation of epithelial cell proliferation involved in lung morphogenesis

Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB

negative regulation of mesenchymal cell proliferation involved in lung development

Inferred from mutant phenotype PubMed 21513708. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 21513708PubMed 19706729. Source: UniProtKB

principal sensory nucleus of trigeminal nerve development

Inferred from mutant phenotype PubMed 19107796. Source: UniProtKB

   Cellular_componentcerebellar mossy fiber

Inferred from direct assay PubMed 19107796. Source: UniProtKB

nucleolus

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 21513708. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 21513708. Source: UniProtKB

RNA polymerase II transcription corepressor activity

Inferred from electronic annotation. Source: Ensembl

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97863-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97863-2)

The sequence of this isoform differs from the canonical sequence as follows:
     416-498: Missing.
Isoform 3 (identifier: P97863-3)

The sequence of this isoform differs from the canonical sequence as follows:
     416-420: PNSSG → SWYLG
     421-570: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Nuclear factor 1 B-type
PRO_0000100197

Regions

DNA binding1 – 195195CTF/NF-I

Amino acid modifications

Modified residue2641Phosphoserine By similarity
Modified residue2921Phosphoserine Ref.6
Modified residue2951Phosphoserine Ref.5 Ref.6
Modified residue3121Phosphoserine By similarity
Modified residue3281Phosphoserine By similarity
Modified residue3331Phosphoserine By similarity

Natural variations

Alternative sequence416 – 49883Missing in isoform 2.
VSP_003547
Alternative sequence416 – 4205PNSSG → SWYLG in isoform 3.
VSP_003548
Alternative sequence421 – 570150Missing in isoform 3.
VSP_003549
Natural variant1311D → G in strain: NIH Swiss.

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: D94AB4400A2811CC

FASTA57063,507
        10         20         30         40         50         60 
MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 

        70         80         90        100        110        120 
LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC 

       130        140        150        160        170        180 
LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL 

       190        200        210        220        230        240 
AYYVQEQDSG QSGSPSHSDP AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP 

       250        260        270        280        290        300 
IGEIPSQPYY HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPNSPAAGS 

       310        320        330        340        350        360 
RTWHERDQDM SSPTTMKKPE KPLFSSTSPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP 

       370        380        390        400        410        420 
PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQPNSSG 

       430        440        450        460        470        480 
QVVGKVPGHF TPVLAPSPHP SAVRPVTLTM TDTKPITTST EGEAASPTAT TYTASGTSQA 

       490        500        510        520        530        540 
NRYVGLSPRD PSFLHQQQLR ICDWTMNQNG RHLYPSTSED TLGITWQSPG TWASLVPFQV 

       550        560        570 
SNRTPILPAN VQNYGLNIIG EPFLQAETSN 

« Hide

Isoform 2 [UniParc].

Checksum: 868043916A36234F
Show »

FASTA48754,908
Isoform 3 [UniParc].

Checksum: E26259E1F965146D
Show »

FASTA42047,472

References

« Hide 'large scale' references
[1]"Expression patterns of the four nuclear factor I genes during mouse embryogenesis indicate a potential role in development."
Chaudhry A.Z., Lyons G.E., Gronostajski R.M.
Dev. Dyn. 208:313-325(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
Strain: BALB/c.
Tissue: Liver and Skeletal muscle.
[2]"Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor Nuclear Factor One."
Gruender A., Qian F., Ebel T.T., Mincheva A., Lichter P., Kruse U., Sippel A.E.
Gene 304:171-181(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3).
Strain: NIH Swiss.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"Exon structure of the nuclear factor I DNA-binding domain from C. elegans to mammals."
Fletcher C.F., Jenkins N.A., Copeland N.G., Chaudhry A.Z., Gronostajski R.M.
Mamm. Genome 10:390-396(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-187.
Strain: 129.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-295, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57634 mRNA. Translation: AAB49929.1.
Y07685 mRNA. Translation: CAA68949.1.
Y07686 mRNA. Translation: CAA68950.1.
Y07687 mRNA. Translation: CAA68951.1.
BC014290 mRNA. Translation: AAH14290.1.
AF111264 Genomic DNA. Translation: AAD39099.1.
CCDSCCDS38790.1. [P97863-3]
CCDS51213.1. [P97863-1]
RefSeqNP_001106680.1. NM_001113209.2. [P97863-1]
NP_032713.3. NM_008687.6. [P97863-3]
UniGeneMm.317947.

3D structure databases

ProteinModelPortalP97863.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP97863. 2 interactions.
MINTMINT-4103630.

PTM databases

PhosphoSiteP97863.

Proteomic databases

MaxQBP97863.
PaxDbP97863.
PRIDEP97863.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050872; ENSMUSP00000052863; ENSMUSG00000008575. [P97863-1]
ENSMUST00000064770; ENSMUSP00000067629; ENSMUSG00000008575. [P97863-3]
GeneID18028.
KEGGmmu:18028.
UCSCuc008tjz.2. mouse. [P97863-1]
uc008tkd.2. mouse. [P97863-2]

Organism-specific databases

CTD4781.
MGIMGI:103188. Nfib.

Phylogenomic databases

eggNOGNOG252172.
GeneTreeENSGT00390000009905.
HOGENOMHOG000013028.
HOVERGENHBG006561.
InParanoidP97863.
KOK09169.
OMAYYVQDQD.
PhylomeDBP97863.
TreeFamTF313889.

Gene expression databases

ArrayExpressP97863.
BgeeP97863.
CleanExMM_NFIB.
GenevestigatorP97863.

Family and domain databases

InterProIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERPTHR11492. PTHR11492. 1 hit.
PfamPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTSM00523. DWA. 1 hit.
[Graphical view]
PROSITEPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNFIB. mouse.
NextBio293099.
PROP97863.
SOURCESearch...

Entry information

Entry nameNFIB_MOUSE
AccessionPrimary (citable) accession number: P97863
Secondary accession number(s): P70252 expand/collapse secondary AC list , P70253, P70254, Q9R1G4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot