Reviewed,
UniProtKB/Swiss-Prot P97857 (ATS1_MOUSE)
Last modified
June 16, 2009.
Version 96.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: A disintegrin and metalloproteinase with thrombospondin motifs 1 Short name=ADAMTS-1 Short name=ADAM-TS 1 Short name=ADAM-TS1 EC=3.4.24.- | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 968 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity By similarity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture By similarity. |
| Catalytic activity | Cleaves aggrecan at the 1691-Glu-|-Leu-1692 site, within the chondroitin sulfate attachment domain. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Subcellular location | |
| Induction | Induced in vitro in colon adenocarcinoma cells by interleukin-1, or in vivo in kidney and heart by lipopolysaccharide. Also induced by LH stimulation in granulosa cells of preovulatory follicles. Ref.6 |
| Domain | The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. |
| Post-translational modification | The precursor is cleaved by a furin endopeptidase. |
| Sequence similarities | Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 3 TSP type-1 domains. |
| Sequence caution | The sequence BAA11088.1 differs from that shown. Reason: Frameshift at position 7. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 48 | 48 | Potential | ||||||||
| Propeptide | 49 – 253 | 205 | PRO_0000029152 | ||||||||
| Chain | 254 – 968 | 715 | A disintegrin and metalloproteinase with thrombospondin motifs 1 | PRO_0000029153 | |||||||
Regions | |||||||||||
| Domain | 259 – 468 | 210 | Peptidase M12B | ||||||||
| Domain | 477 – 559 | 83 | Disintegrin | ||||||||
| Domain | 560 – 615 | 56 | TSP type-1 1 | ||||||||
| Domain | 855 – 911 | 57 | TSP type-1 2 | ||||||||
| Domain | 912 – 968 | 57 | TSP type-1 3 | ||||||||
| Region | 726 – 850 | 125 | Spacer | ||||||||
| Motif | 204 – 211 | 8 | Cysteine switch By similarity | ||||||||
| Compositional bias | 195 – 199 | 5 | Poly-Arg | ||||||||
| Compositional bias | 618 – 725 | 108 | Cys-rich | ||||||||
Sites | |||||||||||
| Active site | 403 | 1 | |||||||||
| Metal binding | 206 | 1 | Zinc; in inhibited form By similarity | ||||||||
| Metal binding | 402 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 406 | 1 | Zinc; catalytic By similarity | ||||||||
| Metal binding | 412 | 1 | Zinc; catalytic By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 548 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 721 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 765 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 783 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 946 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 380 ↔ 463 | By similarity | |||||||||
| Disulfide bond | 418 ↔ 447 | By similarity | |||||||||
| Disulfide bond | 572 ↔ 609 | By similarity | |||||||||
| Disulfide bond | 576 ↔ 614 | By similarity | |||||||||
| Disulfide bond | 587 ↔ 599 | By similarity | |||||||||
Experimental info | |||||||||||
| Mutagenesis | 403 | 1 | E → Q: Loss of activity. Ref.4 | ||||||||
| Sequence conflict | 335 | 1 | N → S in BAA11088. Ref.2 | ||||||||
| Sequence conflict | 425 | 1 | T → S in BAA11088. Ref.2 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The exon/intron organization and chromosomal mapping of the mouse ADAMTS-1 gene encoding an ADAM family protein with TSP motifs." Kuno K., Lizasa H., Ohno S., Matsushima K. Genomics 46:466-471(1997) [PubMed: 9441751] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. |
| [2] | "Molecular cloning of a gene encoding a new type of metalloproteinase-disintegrin family protein with thrombospondin motifs as an inflammation associated gene." Kuno K., Kanada N., Nakashima E., Fujiki F., Ichimura F., Matsushima K. J. Biol. Chem. 272:556-562(1997) [PubMed: 8995297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Limb and Mammary gland. |
| [4] | "ADAMTS-1 is an active metalloproteinase associated with the extracellular matrix." Kuno K., Terashima Y., Matsushima K. J. Biol. Chem. 274:18821-18826(1999) [PubMed: 10373500] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF GLU-403. |
| [5] | "ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan." Kuno K., Okada Y., Kawashima H., Nakamura H., Miyasaka M., Ohno H., Matsushima K. FEBS Lett. 478:241-245(2000) [PubMed: 10930576] [Abstract] Cited for: FUNCTION. |
| [6] | "Progesterone-regulated genes in the ovulation process: ADAMTS-1 and cathepsin L proteases." Robker R.L., Russell D.L., Espey L.L., Lydon J.P., O'Malley B.W., Richards J.S. Proc. Natl. Acad. Sci. U.S.A. 97:4689-4694(2000) [PubMed: 10781075] [Abstract] Cited for: FUNCTION, INDUCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB001735 Genomic DNA. Translation: BAA24501.1. Different initiation. D67076 mRNA. Translation: BAA11088.1. Frameshift. BC040382 mRNA. Translation: AAH40382.1. BC050834 mRNA. Translation: AAH50834.1. | |
| IPI | IPI00130099. |
| PIR | T00017. |
| RefSeq | NP_033751.3. |
| UniGene | Mm.1421 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LSL based on UniProtKB P07996. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | M12.222. |
PTM databases | |
| PhosphoSite | P97857. |
Proteomic databases | |
| PRIDE | P97857. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000022893. Mus musculus. [Contig view] |
| GeneID | 11504. |
| KEGG | mmu:11504. |
Organism-specific databases | |
| MGI | MGI:109249. Adamts1. |
Phylogenomic databases | |
| HOGENOM | P97857. |
| HOVERGEN | P97857. |
Gene expression databases | |
| ArrayExpress | P97857. |
| Bgee | P97857. |
| GermOnline | ENSMUSG00000022893. Mus musculus. |
Family and domain databases | |
| InterPro | IPR006586. ADAM_Cys-rich. IPR010294. ADAM_spacer1. IPR018358. Disintegrin_CS. IPR013274. Pept_M12B_ADAM-TS1. IPR006025. Pept_M_Zn_BS. IPR001590. Peptidase_M12B. IPR013273. Peptidase_M12B_ADAM-TS. IPR002870. Peptidase_M12B_N. IPR000884. Thrombospondin_1_rpt. IPR008085. Thrombospondin_1_rpt_sub. [Graphical view] |
| Pfam | PF05986. ADAM_spacer1. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. PF00090. TSP_1. 3 hits. [Graphical view] |
| PRINTS | PR01858. ADAMTS1. PR01857. ADAMTSFAMILY. PR01705. TSP1REPEAT. |
| SMART | SM00608. ACR. 1 hit. SM00209. TSP1. 3 hits. [Graphical view] |
| PROSITE | PS50215. ADAM_MEPRO. 1 hit. PS00427. DISINTEGRIN_1. False negative. PS50214. DISINTEGRIN_2. False negative. PS50092. TSP1. 3 hits. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 278910. |
| SOURCE | Search... |
Entry information
| Entry name | ATS1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P97857 Secondary accession number(s): O54768 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


