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Protein

Contactin-associated protein 1

Gene

Cntnap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.

GO - Molecular functioni

  • SH3 domain binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Contactin-associated protein 1
Short name:
Caspr
Short name:
Caspr1
Alternative name(s):
Neurexin IV
Neurexin-4
Paranodin
p190
Gene namesi
Name:Cntnap1
Synonyms:Caspr, Nrxn4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi70902. Cntnap1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 12841264ExtracellularSequence analysisAdd
BLAST
Transmembranei1285 – 130521HelicalSequence analysisAdd
BLAST
Topological domaini1306 – 138176CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: RGD
  • integral component of membrane Source: RGD
  • myelin sheath Source: Ensembl
  • paranode region of axon Source: BHF-UCL
  • voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 13811361Contactin-associated protein 1PRO_0000019505Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 169By similarity
Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence analysis
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence analysis
Disulfide bondi324 ↔ 356By similarity
Glycosylationi421 – 4211N-linked (GlcNAc...)Sequence analysis
Glycosylationi500 – 5001N-linked (GlcNAc...)Sequence analysis
Disulfide bondi507 ↔ 539By similarity
Glycosylationi519 – 5191N-linked (GlcNAc...)Sequence analysis
Disulfide bondi545 ↔ 556By similarity
Disulfide bondi550 ↔ 565By similarity
Disulfide bondi567 ↔ 577By similarity
Glycosylationi598 – 5981N-linked (GlcNAc...)Sequence analysis
Glycosylationi654 – 6541N-linked (GlcNAc...)Sequence analysis
Glycosylationi665 – 6651N-linked (GlcNAc...)Sequence analysis
Glycosylationi764 – 7641N-linked (GlcNAc...)Sequence analysis
Glycosylationi805 – 8051N-linked (GlcNAc...)Sequence analysis
Glycosylationi844 – 8441N-linked (GlcNAc...)Sequence analysis
Glycosylationi861 – 8611N-linked (GlcNAc...)Sequence analysis
Disulfide bondi931 ↔ 958By similarity
Glycosylationi949 – 9491N-linked (GlcNAc...)Sequence analysis
Glycosylationi957 – 9571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi962 ↔ 975By similarity
Disulfide bondi969 ↔ 984By similarity
Disulfide bondi986 ↔ 996By similarity
Glycosylationi1079 – 10791N-linked (GlcNAc...)Sequence analysis
Glycosylationi1148 – 11481N-linked (GlcNAc...)Sequence analysis
Disulfide bondi1210 ↔ 1251By similarity
Modified residuei1380 – 13801PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97846.
PRIDEiP97846.

PTM databases

iPTMnetiP97846.
PhosphoSiteiP97846.
UniCarbKBiP97846.

Expressioni

Tissue specificityi

Predominantly expressed in brain. In myelinated nerve fibers of the CNS predominantly found in paranodal axoglial junctions. In unmyelinated nerve fibers of the CNS diffusely distributed along the entire surface. Weak expression is detected in ovary, pancreas, colon, lung, heart, intestine and testis.1 Publication

Developmental stagei

Detected on postnatal day 7 in cerebellum. Follows a caudorostral progession according to the myelination process. Appears to redistribute from the internode to the paranodal region during myelin compaction and maturation. Expression reaches maximal levels between days 14 and 18 and remains at the same levels until adulthood.

Gene expression databases

GenevisibleiP97846. RN.

Interactioni

Subunit structurei

Interacts with contactin in cis form.1 Publication

GO - Molecular functioni

  • SH3 domain binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027505.

Structurei

3D structure databases

ProteinModelPortaliP97846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 169144F5/8 type CPROSITE-ProRule annotationAdd
BLAST
Domaini204 – 356153Laminin G-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini390 – 539150Laminin G-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini544 – 57633EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini577 – 796220Fibrinogen C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini814 – 958145Laminin G-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini962 – 99635EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini1089 – 1251163Laminin G-like 4PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1329 – 136638SH3-bindingSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the neurexin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation
Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation
Contains 4 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, SH3-binding, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3516. Eukaryota.
ENOG410XPHG. LUCA.
GeneTreeiENSGT00760000118991.
HOGENOMiHOG000230964.
HOVERGENiHBG057718.
InParanoidiP97846.
KOiK07379.
OMAiRHDLHYH.
OrthoDBiEOG7GXP9N.
PhylomeDBiP97846.

Family and domain databases

Gene3Di2.60.120.200. 5 hits.
2.60.120.260. 1 hit.
3.90.215.10. 1 hit.
InterProiIPR028872. Caspr1.
IPR013320. ConA-like_dom.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR008979. Galactose-bd-like.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
[Graphical view]
PANTHERiPTHR10127:SF4. PTHR10127:SF4. 2 hits.
PfamiPF00754. F5_F8_type_C. 1 hit.
PF02210. Laminin_G_2. 4 hits.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00231. FA58C. 1 hit.
SM00282. LamG. 4 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 5 hits.
SSF56496. SSF56496. 1 hit.
PROSITEiPS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
PS50025. LAM_G_DOMAIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSLRLFSIL LAAVVSGAQG WGYYGCNEEL VGPLYARSLG ASSYYGLFTT
60 70 80 90 100
ARFARLHGIS GWSPRIGDPN PWLQIDLMKK HRIRAVATQG AFNSWDWVTR
110 120 130 140 150
YMLLYGDRVD SWTPFYQQGH NATFFGNVND SAVVRHDLHY HFTARYIRIV
160 170 180 190 200
PLAWNPRGKI GLRLGIYGCP YTSNILYFDG DDAISYRFQR GASQSLWDVF
210 220 230 240 250
AFSFKTEEKD GLLLHTEGSQ GDYVTLELQG AHLLLHMSLG SSPIQPRPGH
260 270 280 290 300
TTVSAGGVLN DLSWHYVRVD RYGREANLTL DGYVHRFVLN GDFERLNLEN
310 320 330 340 350
EIFIGGLVGA ARKNLAYRHN FRGCIENVIY NRINIAEMAV QRHSRITFEG
360 370 380 390 400
NVAFRCLDPV PHPINFGGPH NFVQVPGFPR RGRLAVSFRF RTWDLTGLLL
410 420 430 440 450
FSRLGDGLGH VELMLSEGQV NVSIAQTGRK KLQFAAGYRL NDGFWHEVNF
460 470 480 490 500
VAQENHAVIS IDDVEGAEVR VSYPLLIRTG TSYFFGGCPK PASRWGCHSN
510 520 530 540 550
QTAFHGCMEL LKVDGQLVNL TLVEFRKLGY FAEVLFDTCG ITDRCSPNMC
560 570 580 590 600
EHDGRCYQSW DDFICYCELT GYKGVTCHEP LYKESCEAYR LSGKYSGNYT
610 620 630 640 650
IDPDGSGPLK PFVVYCDIRE NRAWTVVRHD RLWTTRVTGS SMDRPFLGAI
660 670 680 690 700
QYWNASWEEV SALANASQHC EQWIEFSCYN SRLLNTAGGY PYSFWIGRNE
710 720 730 740 750
EQHFYWGGSQ PGIQRCACGL DQSCIDPALH CNCDADQPQW RTDKGLLTFV
760 770 780 790 800
DHLPVTQVVI GDTNRSSSEA QFFLRPLRCY GDRNSWNTIS FRTGAALRFP
810 820 830 840 850
PIRANHSLDV SFYFRTSAPS GVFLENMGGP FCQWRRPYVR VELNTSRDVV
860 870 880 890 900
FAFDIGNGDE NLTVHSDDFE FNDDEWHLVR AEINVKQARL RVDHRPWVLR
910 920 930 940 950
PMPLQTYIWL EYDQPLYVGS AELKRRPFVG CLRAMRLNGV TLNLEGRANA
960 970 980 990 1000
SEGTFPNCTG HCTHPRFPCF HGGRCVERYS YYTCDCDLTA FDGPYCNHDI
1010 1020 1030 1040 1050
GGFFETGTWM RYNLQSALRS AAQEFSHMLS RPVPGYEPGY IPGYDTPGYV
1060 1070 1080 1090 1100
PGYHGPGYRL PDYPRPGRPV PGYRGPVYNV TGEEVSFSFS TSSAPAVLLY
1110 1120 1130 1140 1150
VSSFVRDYMA VLIKEDGTLQ LRYQLGTSPY VYQLTTRPVT DGQPHSVNIT
1160 1170 1180 1190 1200
RVYRNLFIQV DYFPLTEQKF SLLVDSQLDS PKALYLGRVM ETGVIDPEIQ
1210 1220 1230 1240 1250
RYNTPGFSGC LSGVRFNNVA PLKTHFRTPR PMTAELAEAM RVQGELSESN
1260 1270 1280 1290 1300
CGAMPRLVSE VPPELDPWYL PPDFPYYHDD GWIAILLGFL VAFLLLGLVG
1310 1320 1330 1340 1350
MLVLFYLQNH RYKGSYHTNE PKATHDSHPG GKAPLPPSGP AQAPAPTPAP
1360 1370 1380
TQVPTPAPAP ASGPGPRDQN LPQILEESRS E
Length:1,381
Mass (Da):155,868
Last modified:May 1, 1997 - v1
Checksum:iBC1CE83DB57C1BA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87224 mRNA. Translation: AAB48482.1.
AF000114 mRNA. Translation: AAC53342.1.
PIRiT31083.
RefSeqiNP_114450.1. NM_032061.2.
UniGeneiRn.88654.

Genome annotation databases

EnsembliENSRNOT00000027505; ENSRNOP00000027505; ENSRNOG00000020277.
GeneIDi84008.
KEGGirno:84008.
UCSCiRGD:70902. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87224 mRNA. Translation: AAB48482.1.
AF000114 mRNA. Translation: AAC53342.1.
PIRiT31083.
RefSeqiNP_114450.1. NM_032061.2.
UniGeneiRn.88654.

3D structure databases

ProteinModelPortaliP97846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027505.

PTM databases

iPTMnetiP97846.
PhosphoSiteiP97846.
UniCarbKBiP97846.

Proteomic databases

PaxDbiP97846.
PRIDEiP97846.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027505; ENSRNOP00000027505; ENSRNOG00000020277.
GeneIDi84008.
KEGGirno:84008.
UCSCiRGD:70902. rat.

Organism-specific databases

CTDi8506.
RGDi70902. Cntnap1.

Phylogenomic databases

eggNOGiKOG3516. Eukaryota.
ENOG410XPHG. LUCA.
GeneTreeiENSGT00760000118991.
HOGENOMiHOG000230964.
HOVERGENiHBG057718.
InParanoidiP97846.
KOiK07379.
OMAiRHDLHYH.
OrthoDBiEOG7GXP9N.
PhylomeDBiP97846.

Miscellaneous databases

PROiP97846.

Gene expression databases

GenevisibleiP97846. RN.

Family and domain databases

Gene3Di2.60.120.200. 5 hits.
2.60.120.260. 1 hit.
3.90.215.10. 1 hit.
InterProiIPR028872. Caspr1.
IPR013320. ConA-like_dom.
IPR000742. EGF-like_dom.
IPR000421. FA58C.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR008979. Galactose-bd-like.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
[Graphical view]
PANTHERiPTHR10127:SF4. PTHR10127:SF4. 2 hits.
PfamiPF00754. F5_F8_type_C. 1 hit.
PF02210. Laminin_G_2. 4 hits.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00231. FA58C. 1 hit.
SM00282. LamG. 4 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 5 hits.
SSF56496. SSF56496. 1 hit.
PROSITEiPS50026. EGF_3. 2 hits.
PS01285. FA58C_1. 1 hit.
PS01286. FA58C_2. 1 hit.
PS50022. FA58C_3. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
PS50025. LAM_G_DOMAIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel contactin-associated transmembrane receptor with multiple domains implicated in protein-protein interactions."
    Peles E., Nativ M., Lustig M., Grumet M., Schilling J., Martinez R., Plowman G.D., Schlessinger J.
    EMBO J. 16:978-988(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CONTACTIN.
    Tissue: Pituitary tumor.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "The axonal membrane protein Caspr, a homologue of neurexin IV, is a component of the septate-like paranodal junctions that assemble during myelination."
    Einheber S., Zanazzi G., Ching W., Scherer S., Milner T.A., Peles E., Salzer J.L.
    J. Cell Biol. 139:1495-1506(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Internodal specializations of myelinated axons in the central nervous system."
    Arroyo E.J., Xu T., Poliak S., Watson M., Peles E., Scherer S.S.
    Cell Tissue Res. 305:53-66(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCNTP1_RAT
AccessioniPrimary (citable) accession number: P97846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: May 1, 1997
Last modified: June 8, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.