P97840 (LEG9_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galectin-9
Short name=Gal-9

Alternative name(s):
36 kDa beta-galactoside-binding lectin
Urate transporter/channel
Short name=UAT

Gene names

Name:

Lgals9

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds galactosides. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death By similarity. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion. Highly selective to the anion urate.
Subcellular location	Cytoplasm By similarity. Secreted By similarity. Note: May also be secreted by a non-classical secretory pathway By similarity.
Tissue specificity	The isoform Long is expressed exclusively in the small intestine.
Domain	Contains two homologous but distinct carbohydrate-binding domains.
Miscellaneous	The LGALS9-like proteins are encoded by a duplicated regions on chromosome 17; there are at least 3 genes coding for galectin-9-like proteins.
Sequence similarities	Contains 2 galectin domains.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cytoplasm Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Lectin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform Long (identifier: P97840-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P97840-2)

The sequence of this isoform differs from the canonical sequence as follows:
     148-179: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Galectin-9		PRO_0000076948
Regions
	Domain	17 – 147	131	Galectin 1
	Domain	226 – 354	129	Galectin 2
	Region	81 – 87	7	Beta-galactoside binding 1 By similarity
	Region	286 – 292	7	Beta-galactoside binding 2 By similarity
Sites
	Binding site	47	1	Beta-galactoside 1 By similarity
	Binding site	60	1	Beta-galactoside 1 By similarity
	Binding site	64	1	Beta-galactoside 1 By similarity
	Binding site	74	1	Beta-galactoside 1 By similarity
	Binding site	266	1	Beta-galactoside 2 By similarity
	Binding site	270	1	Beta-galactoside 2 By similarity
	Binding site	280	1	Beta-galactoside 2 By similarity
Natural variations
	Alternative sequence	148 – 179	32	Missing in isoform Short.		VSP_003098

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 6574F960B2EAF37C	FASTA	354	39,946
	10 20 30 40 50 60 MAFFSTQPPY MNPVIPFTGI IQGGLQNGLQ ITLQGTVHPF PNRIAVNFQT GFSGNDIAFH 70 80 90 100 110 120 FNPRFEEGGY VVCNTKQNGK WGPEERKMQM PFQKGMPFEL CFLVQRSEFK VMVNKNFFVQ 130 140 150 160 170 180 YSHRVPYHLV DTISVSGCLH LSFINFQNST AAPVQPVFST MQFSQPVQFP RMPKGRKQRT 190 200 210 220 230 240 QGFQPALQAP VAQTIIHTVH SIPGQMLSTP GIPPMAYPTP AYTIPFFTSI PNGFYPSKSI 250 260 270 280 290 300 NISGVVLPDA KRFHINLRCG GDIAFHLNPR FNEKVVVRNT QINNSWGPEE RSLPGRMPFN 310 320 330 340 350 RGQSFSVWIL CEGHCFKVAV DGQHICEYYH RLKNLPDINT LEVAGDIQLT HVQT
	Isoform Short [UniParc]. Checksum: 3268B472A91EECCA	FASTA	322	36,317
	10 20 30 40 50 60 MAFFSTQPPY MNPVIPFTGI IQGGLQNGLQ ITLQGTVHPF PNRIAVNFQT GFSGNDIAFH 70 80 90 100 110 120 FNPRFEEGGY VVCNTKQNGK WGPEERKMQM PFQKGMPFEL CFLVQRSEFK VMVNKNFFVQ 130 140 150 160 170 180 YSHRVPYHLV DTISVSGCLH LSFINFQTQG FQPALQAPVA QTIIHTVHSI PGQMLSTPGI 190 200 210 220 230 240 PPMAYPTPAY TIPFFTSIPN GFYPSKSINI SGVVLPDAKR FHINLRCGGD IAFHLNPRFN 250 260 270 280 290 300 EKVVVRNTQI NNSWGPEERS LPGRMPFNRG QSFSVWILCE GHCFKVAVDG QHICEYYHRL 310 320 KNLPDINTLE VAGDIQLTHV QT

References

[1]	"Identification and characterization of galectin-9, a novel beta-galactoside-binding mammalian lectin." Wada J., Kanwar Y.S. J. Biol. Chem. 272:6078-6086(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Strain: Sprague-Dawley. Tissue: Kidney and Small intestine.
[2]	"Molecular cloning and functional reconstitution of a urate transporter/channel." Leal-Pinto E., Tao W., Rappaport J., Richardson M., Knorr B.A., Abramson R.G. J. Biol. Chem. 272:617-625(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Strain: Sprague-Dawley. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59462 mRNA. Translation: AAB51192.1. U72741 mRNA. Translation: AAB68592.1. U67958 mRNA. Translation: AAB48591.1.
IPI	IPI00208696. IPI00325484.
RefSeq	NP_037109.1. NM_012977.1.
UniGene	Rn.10706.
3D structure databases
ProteinModelPortal	P97840.
SMR	P97840. Positions 1-147.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000017042.
Protein family/group databases
TCDB	9.B.9.1.1. urate transporter (UAT) family.
Proteomic databases
PaxDb	P97840.
PRIDE	P97840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25476.
KEGG	rno:25476.
Organism-specific databases
CTD	3965.
RGD	3005. Lgals9.
Phylogenomic databases
eggNOG	NOG264430.
HOGENOM	HOG000290194.
HOVERGEN	HBG002412.
InParanoid	P97840.
KO	K10093.
OrthoDB	EOG49GKH7.
Gene expression databases
ArrayExpress	P97840.
Genevestigator	P97840.
GermOnline	ENSRNOG00000012681. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.120.200. 2 hits.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR001079. Galectin_CRD. [Graphical view]
Pfam	PF00337. Gal-bind_lectin. 2 hits. [Graphical view]
SMART	SM00908. Gal-bind_lectin. 2 hits. SM00276. GLECT. 2 hits. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 2 hits.
PROSITE	PS51304. GALECTIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	606793.

Entry information

Entry name

LEG9_RAT

Accession

Primary (citable) accession number: P97840
Secondary accession number(s): O08588, O35866

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program