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Protein

Disks large-associated protein 3

Gene

Dlgap3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.

GO - Molecular functioni

  • PDZ domain binding Source: BHF-UCL
  • protein complex scaffold Source: BHF-UCL
  • protein domain specific binding Source: RGD
  • scaffold protein binding Source: BHF-UCL

GO - Biological processi

  • protein complex assembly Source: BHF-UCL
  • signaling Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 3
Short name:
DAP-3
Alternative name(s):
PSD-95/SAP90-binding protein 3
SAP90/PSD-95-associated protein 3
Short name:
SAPAP3
Gene namesi
Name:Dlgap3
Synonyms:Dap3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708349. Dlgap3.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • dendritic spine Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 977977Disks large-associated protein 3PRO_0000174296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581PhosphoserineBy similarity
Modified residuei404 – 4041PhosphoserineBy similarity
Modified residuei407 – 4071PhosphoserineBy similarity
Modified residuei410 – 4101PhosphoserineBy similarity
Modified residuei414 – 4141PhosphoserineBy similarity
Modified residuei641 – 6411PhosphoserineBy similarity
Modified residuei643 – 6431PhosphoserineBy similarity
Modified residuei930 – 9301PhosphoserineCombined sources
Modified residuei933 – 9331PhosphoserineCombined sources
Modified residuei965 – 9651PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97838.
PRIDEiP97838.

PTM databases

iPTMnetiP97838.
PhosphoSiteiP97838.

Expressioni

Tissue specificityi

Expressed in most brain regions.2 Publications

Interactioni

Subunit structurei

Interacts with DLG1 and DLG4/PSD-95.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GraspQ8R4T53EBI-375673,EBI-7361884

GO - Molecular functioni

  • PDZ domain binding Source: BHF-UCL
  • protein complex scaffold Source: BHF-UCL
  • protein domain specific binding Source: RGD
  • scaffold protein binding Source: BHF-UCL

Protein-protein interaction databases

IntActiP97838. 6 interactions.
MINTiMINT-1790577.
STRINGi10116.ENSRNOP00000019214.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi222 – 23312Poly-HisAdd
BLAST

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
HOGENOMiHOG000015346.
HOVERGENiHBG018957.
InParanoidiP97838.
PhylomeDBiP97838.

Family and domain databases

InterProiIPR030526. DLGAP3.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF4. PTHR12353:SF4. 3 hits.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97838-1) [UniParc]FASTAAdd to basket

Also known as: SAPAP3+

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGYHGDRGS HPRPARFADQ QHMDVGPAAR APYLLGSREA FSTEPRFCAP
60 70 80 90 100
RAGLGHLSPE GPLSLSEGPS SVGPEGGPGG VGAGGSSSTF PRMYPGQGPF
110 120 130 140 150
DTCEDCVGHP QGKGATRLLP TFLDQFEKQL PVQQDGFHTL PYQRGPAGPG
160 170 180 190 200
PGPGSGAAPE ARSESPSRIR HLVHSVQKLF AKSHSLEAPG KRDYNGPKAE
210 220 230 240 250
GRSSSGGDSY SGPGSGGPPT SHHHHHHHHH HHHQSRHGKR SKSKDRKGDG
260 270 280 290 300
RHQTKATGWW SSDDNLDSDS GFLGGRPPGE PGGPFCLDAP DGSYRDLSFK
310 320 330 340 350
GRSGGSEGRC LACTGMSMSL DGQSVKRSAW HTMMVSQGRD GYPGAGPGKG
360 370 380 390 400
LLGPETKAKA RTYHYLQVPQ DDWGGYPTGG KDGEIPCRRM RSGSYIKAMG
410 420 430 440 450
DEESGDSDGS PKTSPKALAR RFASRRSSSV DTARINCCVP PRIHPRSSIP
460 470 480 490 500
GYSRSLTTGQ LSEEFNQQLE AVCGSVFGEL ESQAVDALDL PGCFRMRSHS
510 520 530 540 550
YLRAIQAGCS QDDDCLPLLA APASVSGRPG SSFNFRKAPP PIPPGSQAPP
560 570 580 590 600
RISITAQSST DSAHESFTAA EGPARRCSSA DGLDGPTMGA RTLELAPVPP
610 620 630 640 650
RASPKPPTLI IKTIPGREEL RSLARQRKWR PSIGVQVETI SDSDTENRSR
660 670 680 690 700
REFHSIGVQV EEDKRRARFK RSNSVTAGVQ ADLELEGLAG LATVATEDKA
710 720 730 740 750
LQFGRPFQRQ ASEPQPGPRA PTYSVFRTVH TQGQWAYREG YPLPYEPPAT
760 770 780 790 800
DGSPGPPPVP APGPGSGRRD SWMERGSRSL PDSGRTSPCP RDGEWFIKML
810 820 830 840 850
RAEVEKLEHW CQQMEREAED YELPEEILEK IRSAVGSTQL LLSQKVQQFF
860 870 880 890 900
RLCQQSLDPT AFPVPTFQDL AGFWDLLQLS IEDVTLKFLE LQQLKANSWK
910 920 930 940 950
LLEPKEEKKV PPPIPKKPSR GRGVPVKERS LDSVDRQRQE ARKRLLAAKR
960 970
AASFRHSSAT ESADSIEIYI PEAQTRL
Length:977
Mass (Da):105,990
Last modified:July 19, 2005 - v2
Checksum:iAD65DB11E1D4F723
GO
Isoform 2 (identifier: P97838-2) [UniParc]FASTAAdd to basket

Also known as: SAPAP3-

The sequence of this isoform differs from the canonical sequence as follows:
     858-868: Missing.

Show »
Length:966
Mass (Da):104,789
Checksum:i4FE546B0E496AA5C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771G → S in AAB48589 (PubMed:9115257).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei858 – 86811Missing in isoform 2. 1 PublicationVSP_014818Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67139 mRNA. Translation: AAB48589.1.
AY530298 mRNA. Translation: AAS90634.1.
AY530299 mRNA. Translation: AAS90635.1.
UniGeneiRn.22954.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67139 mRNA. Translation: AAB48589.1.
AY530298 mRNA. Translation: AAS90634.1.
AY530299 mRNA. Translation: AAS90635.1.
UniGeneiRn.22954.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97838. 6 interactions.
MINTiMINT-1790577.
STRINGi10116.ENSRNOP00000019214.

PTM databases

iPTMnetiP97838.
PhosphoSiteiP97838.

Proteomic databases

PaxDbiP97838.
PRIDEiP97838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi708349. Dlgap3.

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
HOGENOMiHOG000015346.
HOVERGENiHBG018957.
InParanoidiP97838.
PhylomeDBiP97838.

Miscellaneous databases

PROiP97838.

Family and domain databases

InterProiIPR030526. DLGAP3.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF4. PTHR12353:SF4. 3 hits.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density."
    Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y.
    J. Biol. Chem. 272:11943-11951(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH DLG4.
    Tissue: Brain.
  2. "Distinct spatiotemporal expression of SAPAP transcripts in the developing rat brain: a novel dendritically localized mRNA."
    Kindler S., Rehbein M., Classen B., Richter D., Boeckers T.M.
    Brain Res. Mol. Brain Res. 126:14-21(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Tissue: Brain.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-930 AND SER-933, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDLGP3_RAT
AccessioniPrimary (citable) accession number: P97838
Secondary accession number(s): Q6QQA8, Q6QQA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 19, 2005
Last modified: June 8, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.