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P97836

- DLGP1_RAT

UniProt

P97836 - DLGP1_RAT

Protein

Disks large-associated protein 1

Gene

Dlgap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Part of the postsynaptic scaffold in neuronal cells.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein complex binding Source: RGD
    3. protein domain specific binding Source: RGD

    GO - Biological processi

    1. aggresome assembly Source: BHF-UCL
    2. cell-cell signaling Source: InterPro
    3. protein localization to synapse Source: BHF-UCL
    4. regulation of proteasomal protein catabolic process Source: BHF-UCL

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disks large-associated protein 1
    Short name:
    DAP-1
    Alternative name(s):
    Guanylate kinase-associated protein
    Short name:
    rGKAP
    PSD-95/SAP90-binding protein 1
    SAP90/PSD-95-associated protein 1
    Short name:
    SAPAP1
    Gene namesi
    Name:Dlgap1
    Synonyms:Gkap
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620223. Dlgap1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. postsynaptic density Source: UniProtKB-SubCell
    3. postsynaptic membrane Source: RGD
    4. synapse Source: BHF-UCL

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi990 – 9901T → A: Abolishes interaction with SHANK1. 1 Publication
    Mutagenesisi991 – 9911R → D: Abolishes interaction with SHANK1. 1 Publication
    Mutagenesisi992 – 9921L → A: Abolishes interaction with SHANK1. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 992992Disks large-associated protein 1PRO_0000174290Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei362 – 3621PhosphoserineBy similarity
    Modified residuei509 – 5091PhosphoserineBy similarity
    Modified residuei516 – 5161PhosphoserineBy similarity
    Modified residuei947 – 9471PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP97836.
    PRIDEiP97836.

    PTM databases

    PhosphoSiteiP97836.

    Expressioni

    Tissue specificityi

    Expressed in brain and testis.

    Developmental stagei

    Highest level of isoform 1 in the brain of newborn rats. Increasing levels of isoforms 2, 3, 4, and 5 in the brain of newborn rats from birth to 6 weeks of postnatal development. Increasing but low level of isoform 6 is expressed in the brain from 2 to 6 weeks of postnatal development.1 Publication

    Gene expression databases

    GenevestigatoriP97836.

    Interactioni

    Subunit structurei

    Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.8 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DLG4P783523EBI-6269434,EBI-80389From a different organism.
    Dlg4P310169EBI-80901,EBI-375655
    Magi2O883823EBI-80901,EBI-696179
    Shank1Q9WV486EBI-80901,EBI-80909

    Protein-protein interaction databases

    BioGridi249233. 7 interactions.
    IntActiP97836. 15 interactions.
    MINTiMINT-101502.
    STRINGi10116.ENSRNOP00000022351.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni665 – 67612Interaction with DYL2Add
    BLAST
    Regioni687 – 69812Interaction with DYL2Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi990 – 9923PDZ-binding

    Sequence similaritiesi

    Belongs to the SAPAP family.Curated

    Phylogenomic databases

    eggNOGiNOG277836.
    HOGENOMiHOG000015346.
    HOVERGENiHBG018957.
    InParanoidiP97836.
    KOiK15008.
    PhylomeDBiP97836.

    Family and domain databases

    InterProiIPR005026. GKAP.
    [Graphical view]
    PfamiPF03359. GKAP. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P97836-1) [UniParc]FASTAAdd to Basket

    Also known as: SAPAP1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKGLSGSRSH HHGITCESAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR    50
    NSFQAECVGP FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP 100
    TNLLDQFERQ LPLSRDGYHT LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT 150
    KSHSLEGASK GGVNGGKASP DGSQTVRYGK RSKSKERRSE PKARSNASNA 200
    SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSVSQ YFMGAYNTIS 250
    EQAVKASRSN NDVKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK 300
    ASVNMDQAVV KSEACQQERS CQYLQVPQDE WTGYTPRGKD DEIPCRRMRS 350
    GSYIKAMGDE DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN 400
    EHSPKLQIRS HSYLRAVSEV SINRSLDSLD PAGLLTSPKF RSRNESYMRA 450
    MSTISQVSEM EVNGQFESVC ESVFSELESQ AVEALDLPMP GCFRMRSHSY 500
    VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK LSSAVEVSSC 550
    ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS 600
    GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV 650
    TTEDRKKDFK KNRCLSIGIQ VDDAEESEKM AESKTSSKFQ SVGVQVEEEK 700
    CFRRFTRSNS VTTAVQADLD FHDNLENSLE SIEDNSCPGP MARQFSRDAS 750
    TSTVSIQGSG NHYHACAADD DFDTDFDPSI LPPPDPWIDS ITEDPLEAVQ 800
    RSVCHRDGHW FLKLLQAERD RMEGWCKQME REERENNLPE DILGKIRTAV 850
    GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS 900
    MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES 950
    SQRQEARKRL MAAKRAASVR QNSATESAES IEIYIPEAQT RL 992
    Length:992
    Mass (Da):110,178
    Last modified:May 1, 1997 - v1
    Checksum:i44BAF9BC0C14C099
    GO
    Isoform 2 (identifier: P97836-4) [UniParc]FASTAAdd to Basket

    Also known as: GKAP C, 2A

    The sequence of this isoform differs from the canonical sequence as follows:
         1-298: Missing.
         299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW

    Show »
    Length:694
    Mass (Da):77,263
    Checksum:i2263D659AD1F6627
    GO
    Isoform 3 (identifier: P97836-5) [UniParc]FASTAAdd to Basket

    Also known as: GKAP A, GKAP1a

    The sequence of this isoform differs from the canonical sequence as follows:
         1-298: Missing.
         299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
         547-574: Missing.

    Show »
    Length:666
    Mass (Da):74,220
    Checksum:iF027510C20BC2290
    GO
    Isoform 4 (identifier: P97836-6) [UniParc]FASTAAdd to Basket

    Also known as: GKAP B, 2C

    The sequence of this isoform differs from the canonical sequence as follows:
         1-298: Missing.
         299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
         537-546: Missing.

    Show »
    Length:684
    Mass (Da):76,279
    Checksum:iC37FA0BA715BAF66
    GO
    Isoform 5 (identifier: P97836-7) [UniParc]FASTAAdd to Basket

    Also known as: 2B

    The sequence of this isoform differs from the canonical sequence as follows:
         1-298: Missing.
         299-324: QKASVNMDQAVVKSEACQQERSCQYL → MIDLFKAEWVSSVCVQVSRNGRTD

    Show »
    Length:692
    Mass (Da):76,978
    Checksum:i7180180258ADEF72
    GO
    Isoform 6 (identifier: P97836-8) [UniParc]FASTAAdd to Basket

    Also known as: 2D

    The sequence of this isoform differs from the canonical sequence as follows:
         1-298: Missing.
         299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MNLIFHKDILFGVSATK

    Show »
    Length:684
    Mass (Da):76,043
    Checksum:i991D69B760948790
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti636 – 6361S → T in AAC53054. (PubMed:9024696)Curated
    Sequence conflicti636 – 6361S → T in BAA24265. (PubMed:9428732)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 298298Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6. 2 PublicationsVSP_015415Add
    BLAST
    Alternative sequencei299 – 32527QKASV…CQYLQ → MIDLFKAEWVSSVCVQVSRN GRTDQVW in isoform 2, isoform 3 and isoform 4. 2 PublicationsVSP_015416Add
    BLAST
    Alternative sequencei299 – 32527QKASV…CQYLQ → MNLIFHKDILFGVSATK in isoform 6. 1 PublicationVSP_015417Add
    BLAST
    Alternative sequencei299 – 32426QKASV…SCQYL → MIDLFKAEWVSSVCVQVSRN GRTD in isoform 5. 1 PublicationVSP_015418Add
    BLAST
    Alternative sequencei537 – 54610Missing in isoform 4. 2 PublicationsVSP_015419
    Alternative sequencei547 – 57428Missing in isoform 3. 1 PublicationVSP_015420Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67987 mRNA. Translation: AAC53054.1.
    AB003594 mRNA. Translation: BAA24265.1.
    U67137 mRNA. Translation: AAB48587.1.
    PIRiT00025.
    RefSeqiNP_075235.2. NM_022946.2. [P97836-1]
    UniGeneiRn.90059.

    Genome annotation databases

    GeneIDi65040.
    KEGGirno:65040.
    UCSCiRGD:620223. rat. [P97836-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67987 mRNA. Translation: AAC53054.1 .
    AB003594 mRNA. Translation: BAA24265.1 .
    U67137 mRNA. Translation: AAB48587.1 .
    PIRi T00025.
    RefSeqi NP_075235.2. NM_022946.2. [P97836-1 ]
    UniGenei Rn.90059.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249233. 7 interactions.
    IntActi P97836. 15 interactions.
    MINTi MINT-101502.
    STRINGi 10116.ENSRNOP00000022351.

    PTM databases

    PhosphoSitei P97836.

    Proteomic databases

    PaxDbi P97836.
    PRIDEi P97836.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 65040.
    KEGGi rno:65040.
    UCSCi RGD:620223. rat. [P97836-1 ]

    Organism-specific databases

    CTDi 9229.
    RGDi 620223. Dlgap1.

    Phylogenomic databases

    eggNOGi NOG277836.
    HOGENOMi HOG000015346.
    HOVERGENi HBG018957.
    InParanoidi P97836.
    KOi K15008.
    PhylomeDBi P97836.

    Miscellaneous databases

    NextBioi 613856.
    PROi P97836.

    Gene expression databases

    Genevestigatori P97836.

    Family and domain databases

    InterProi IPR005026. GKAP.
    [Graphical view ]
    Pfami PF03359. GKAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "GKAP, a novel synaptic protein that interacts with the guanylate kinase-like domain of the PSD-95/SAP90 family of channel clustering molecules."
      Kim E., Naisbitt S., Hsueh Y.-P., Rao A., Rothschild A., Craig A.M., Sheng M.
      J. Cell Biol. 136:669-678(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), INTERACTION WITH DLG1; DLG2; DLG3 AND DLG4.
      Tissue: Brain.
    2. "Differential expression of isoforms of PSD-95 binding protein (GKAP/SAPAP1) during rat brain development."
      Kawashima N., Takamiya K., Sun J., Kitabatake A., Sobue K.
      FEBS Lett. 418:301-304(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 4; 5 AND 6), DEVELOPMENTAL STAGE.
    3. "SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density."
      Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y.
      J. Biol. Chem. 272:11943-11951(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "BEGAIN (brain-enriched guanylate kinase-associated protein), a novel neuronal PSD-95/SAP90-binding protein."
      Deguchi M., Hata Y., Takeuchi M., Ide N., Hirao K., Yao I., Irie M., Toyoda A., Takai Y.
      J. Biol. Chem. 273:26269-26272(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BEGAIN AND DLG4.
    5. "Proline-rich synapse-associated proteins ProSAP1 and ProSAP2 interact with synaptic proteins of the SAPAP/GKAP family."
      Boeckers T.M., Winter C., Smalla K.-H., Kreutz M.R., Bockmann J., Seidenbecher C., Garner C.C., Gundelfinger E.D.
      Biochem. Biophys. Res. Commun. 264:247-252(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLG4 AND SHANK PROTEINS.
    6. "Synamon, a novel neuronal protein interacting with synapse-associated protein 90/postsynaptic density-95-associated protein."
      Yao I., Hata Y., Hirao K., Deguchi M., Ide N., Takeuchi M., Takai Y.
      J. Biol. Chem. 274:27463-27466(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLG4 AND SHANK1.
      Tissue: Brain.
    7. "Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin."
      Naisbitt S., Kim E., Tu J.C., Xiao B., Sala C., Valtschanoff J., Weinberg R.J., Worley P.F., Sheng M.
      Neuron 23:569-582(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHANK PROTEINS, MUTAGENESIS OF THR-990; ARG-991 AND LEU-992.
    8. "A novel multiple PDZ domain-containing molecule interacting with N-methyl-d-aspartate receptors and neuronal cell adhesion proteins."
      Hirao K., Hata Y., Ide N., Takeuchi M., Irie M., Yao I., Deguchi M., Toyoda A., Suedhof T.C., Takai Y.
      J. Biol. Chem. 273:21105-21110(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AIP1.
    9. "Proteomic identification of brain proteins that interact with dynein light chain LC8."
      Navarro-Lerida I., Martinez Moreno M., Roncal F., Gavilanes F., Albar J.P., Rodriguez-Crespo I.
      Proteomics 4:339-346(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DYL2.
    10. "SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses."
      Ko J., Kim S., Chung H.S., Kim K., Han K., Kim H., Jun H., Kaang B.-K., Kim E.
      Neuron 50:233-245(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LRFN1.

    Entry informationi

    Entry nameiDLGP1_RAT
    AccessioniPrimary (citable) accession number: P97836
    Secondary accession number(s): O54773, P97841
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3