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P97836

- DLGP1_RAT

UniProt

P97836 - DLGP1_RAT

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Protein
Disks large-associated protein 1
Gene
Dlgap1, Gkap
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the postsynaptic scaffold in neuronal cells.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein complex binding Source: RGD
  3. protein domain specific binding Source: RGD

GO - Biological processi

  1. aggresome assembly Source: BHF-UCL
  2. cell-cell signaling Source: InterPro
  3. protein localization to synapse Source: BHF-UCL
  4. regulation of proteasomal protein catabolic process Source: BHF-UCL
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 1
Short name:
DAP-1
Alternative name(s):
Guanylate kinase-associated protein
Short name:
rGKAP
PSD-95/SAP90-binding protein 1
SAP90/PSD-95-associated protein 1
Short name:
SAPAP1
Gene namesi
Name:Dlgap1
Synonyms:Gkap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620223. Dlgap1.

Subcellular locationi

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. postsynaptic density Source: UniProtKB-SubCell
  3. postsynaptic membrane Source: RGD
  4. synapse Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi990 – 9901T → A: Abolishes interaction with SHANK1. 1 Publication
Mutagenesisi991 – 9911R → D: Abolishes interaction with SHANK1. 1 Publication
Mutagenesisi992 – 9921L → A: Abolishes interaction with SHANK1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 992992Disks large-associated protein 1
PRO_0000174290Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei362 – 3621Phosphoserine By similarity
Modified residuei509 – 5091Phosphoserine By similarity
Modified residuei516 – 5161Phosphoserine By similarity
Modified residuei947 – 9471Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97836.
PRIDEiP97836.

PTM databases

PhosphoSiteiP97836.

Expressioni

Tissue specificityi

Expressed in brain and testis.

Developmental stagei

Highest level of isoform 1 in the brain of newborn rats. Increasing levels of isoforms 2, 3, 4, and 5 in the brain of newborn rats from birth to 6 weeks of postnatal development. Increasing but low level of isoform 6 is expressed in the brain from 2 to 6 weeks of postnatal development.1 Publication

Gene expression databases

GenevestigatoriP97836.

Interactioni

Subunit structurei

Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DLG4P783523EBI-6269434,EBI-80389From a different organism.
Dlg4P310169EBI-80901,EBI-375655
Magi2O883823EBI-80901,EBI-696179
Shank1Q9WV486EBI-80901,EBI-80909

Protein-protein interaction databases

BioGridi249233. 7 interactions.
IntActiP97836. 15 interactions.
MINTiMINT-101502.
STRINGi10116.ENSRNOP00000022351.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni665 – 67612Interaction with DYL2
Add
BLAST
Regioni687 – 69812Interaction with DYL2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi990 – 9923PDZ-binding

Sequence similaritiesi

Belongs to the SAPAP family.

Phylogenomic databases

eggNOGiNOG277836.
HOGENOMiHOG000015346.
HOVERGENiHBG018957.
InParanoidiP97836.
KOiK15008.
PhylomeDBiP97836.

Family and domain databases

InterProiIPR005026. GKAP.
[Graphical view]
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97836-1) [UniParc]FASTAAdd to Basket

Also known as: SAPAP1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKGLSGSRSH HHGITCESAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR    50
NSFQAECVGP FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP 100
TNLLDQFERQ LPLSRDGYHT LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT 150
KSHSLEGASK GGVNGGKASP DGSQTVRYGK RSKSKERRSE PKARSNASNA 200
SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSVSQ YFMGAYNTIS 250
EQAVKASRSN NDVKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK 300
ASVNMDQAVV KSEACQQERS CQYLQVPQDE WTGYTPRGKD DEIPCRRMRS 350
GSYIKAMGDE DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN 400
EHSPKLQIRS HSYLRAVSEV SINRSLDSLD PAGLLTSPKF RSRNESYMRA 450
MSTISQVSEM EVNGQFESVC ESVFSELESQ AVEALDLPMP GCFRMRSHSY 500
VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK LSSAVEVSSC 550
ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS 600
GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV 650
TTEDRKKDFK KNRCLSIGIQ VDDAEESEKM AESKTSSKFQ SVGVQVEEEK 700
CFRRFTRSNS VTTAVQADLD FHDNLENSLE SIEDNSCPGP MARQFSRDAS 750
TSTVSIQGSG NHYHACAADD DFDTDFDPSI LPPPDPWIDS ITEDPLEAVQ 800
RSVCHRDGHW FLKLLQAERD RMEGWCKQME REERENNLPE DILGKIRTAV 850
GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS 900
MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES 950
SQRQEARKRL MAAKRAASVR QNSATESAES IEIYIPEAQT RL 992
Length:992
Mass (Da):110,178
Last modified:May 1, 1997 - v1
Checksum:i44BAF9BC0C14C099
GO
Isoform 2 (identifier: P97836-4) [UniParc]FASTAAdd to Basket

Also known as: GKAP C, 2A

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW

Show »
Length:694
Mass (Da):77,263
Checksum:i2263D659AD1F6627
GO
Isoform 3 (identifier: P97836-5) [UniParc]FASTAAdd to Basket

Also known as: GKAP A, GKAP1a

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
     547-574: Missing.

Show »
Length:666
Mass (Da):74,220
Checksum:iF027510C20BC2290
GO
Isoform 4 (identifier: P97836-6) [UniParc]FASTAAdd to Basket

Also known as: GKAP B, 2C

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
     537-546: Missing.

Show »
Length:684
Mass (Da):76,279
Checksum:iC37FA0BA715BAF66
GO
Isoform 5 (identifier: P97836-7) [UniParc]FASTAAdd to Basket

Also known as: 2B

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-324: QKASVNMDQAVVKSEACQQERSCQYL → MIDLFKAEWVSSVCVQVSRNGRTD

Show »
Length:692
Mass (Da):76,978
Checksum:i7180180258ADEF72
GO
Isoform 6 (identifier: P97836-8) [UniParc]FASTAAdd to Basket

Also known as: 2D

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MNLIFHKDILFGVSATK

Show »
Length:684
Mass (Da):76,043
Checksum:i991D69B760948790
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 298298Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6.
VSP_015415Add
BLAST
Alternative sequencei299 – 32527QKASV…CQYLQ → MIDLFKAEWVSSVCVQVSRN GRTDQVW in isoform 2, isoform 3 and isoform 4.
VSP_015416Add
BLAST
Alternative sequencei299 – 32527QKASV…CQYLQ → MNLIFHKDILFGVSATK in isoform 6.
VSP_015417Add
BLAST
Alternative sequencei299 – 32426QKASV…SCQYL → MIDLFKAEWVSSVCVQVSRN GRTD in isoform 5.
VSP_015418Add
BLAST
Alternative sequencei537 – 54610Missing in isoform 4.
VSP_015419
Alternative sequencei547 – 57428Missing in isoform 3.
VSP_015420Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti636 – 6361S → T in AAC53054. 1 Publication
Sequence conflicti636 – 6361S → T in BAA24265. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U67987 mRNA. Translation: AAC53054.1.
AB003594 mRNA. Translation: BAA24265.1.
U67137 mRNA. Translation: AAB48587.1.
PIRiT00025.
RefSeqiNP_075235.2. NM_022946.2. [P97836-1]
UniGeneiRn.90059.

Genome annotation databases

GeneIDi65040.
KEGGirno:65040.
UCSCiRGD:620223. rat. [P97836-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U67987 mRNA. Translation: AAC53054.1 .
AB003594 mRNA. Translation: BAA24265.1 .
U67137 mRNA. Translation: AAB48587.1 .
PIRi T00025.
RefSeqi NP_075235.2. NM_022946.2. [P97836-1 ]
UniGenei Rn.90059.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249233. 7 interactions.
IntActi P97836. 15 interactions.
MINTi MINT-101502.
STRINGi 10116.ENSRNOP00000022351.

PTM databases

PhosphoSitei P97836.

Proteomic databases

PaxDbi P97836.
PRIDEi P97836.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 65040.
KEGGi rno:65040.
UCSCi RGD:620223. rat. [P97836-1 ]

Organism-specific databases

CTDi 9229.
RGDi 620223. Dlgap1.

Phylogenomic databases

eggNOGi NOG277836.
HOGENOMi HOG000015346.
HOVERGENi HBG018957.
InParanoidi P97836.
KOi K15008.
PhylomeDBi P97836.

Miscellaneous databases

NextBioi 613856.
PROi P97836.

Gene expression databases

Genevestigatori P97836.

Family and domain databases

InterProi IPR005026. GKAP.
[Graphical view ]
Pfami PF03359. GKAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "GKAP, a novel synaptic protein that interacts with the guanylate kinase-like domain of the PSD-95/SAP90 family of channel clustering molecules."
    Kim E., Naisbitt S., Hsueh Y.-P., Rao A., Rothschild A., Craig A.M., Sheng M.
    J. Cell Biol. 136:669-678(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), INTERACTION WITH DLG1; DLG2; DLG3 AND DLG4.
    Tissue: Brain.
  2. "Differential expression of isoforms of PSD-95 binding protein (GKAP/SAPAP1) during rat brain development."
    Kawashima N., Takamiya K., Sun J., Kitabatake A., Sobue K.
    FEBS Lett. 418:301-304(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 4; 5 AND 6), DEVELOPMENTAL STAGE.
  3. "SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density."
    Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y.
    J. Biol. Chem. 272:11943-11951(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "BEGAIN (brain-enriched guanylate kinase-associated protein), a novel neuronal PSD-95/SAP90-binding protein."
    Deguchi M., Hata Y., Takeuchi M., Ide N., Hirao K., Yao I., Irie M., Toyoda A., Takai Y.
    J. Biol. Chem. 273:26269-26272(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BEGAIN AND DLG4.
  5. "Proline-rich synapse-associated proteins ProSAP1 and ProSAP2 interact with synaptic proteins of the SAPAP/GKAP family."
    Boeckers T.M., Winter C., Smalla K.-H., Kreutz M.R., Bockmann J., Seidenbecher C., Garner C.C., Gundelfinger E.D.
    Biochem. Biophys. Res. Commun. 264:247-252(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG4 AND SHANK PROTEINS.
  6. "Synamon, a novel neuronal protein interacting with synapse-associated protein 90/postsynaptic density-95-associated protein."
    Yao I., Hata Y., Hirao K., Deguchi M., Ide N., Takeuchi M., Takai Y.
    J. Biol. Chem. 274:27463-27466(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG4 AND SHANK1.
    Tissue: Brain.
  7. "Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin."
    Naisbitt S., Kim E., Tu J.C., Xiao B., Sala C., Valtschanoff J., Weinberg R.J., Worley P.F., Sheng M.
    Neuron 23:569-582(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHANK PROTEINS, MUTAGENESIS OF THR-990; ARG-991 AND LEU-992.
  8. "A novel multiple PDZ domain-containing molecule interacting with N-methyl-d-aspartate receptors and neuronal cell adhesion proteins."
    Hirao K., Hata Y., Ide N., Takeuchi M., Irie M., Yao I., Deguchi M., Toyoda A., Suedhof T.C., Takai Y.
    J. Biol. Chem. 273:21105-21110(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AIP1.
  9. "Proteomic identification of brain proteins that interact with dynein light chain LC8."
    Navarro-Lerida I., Martinez Moreno M., Roncal F., Gavilanes F., Albar J.P., Rodriguez-Crespo I.
    Proteomics 4:339-346(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYL2.
  10. "SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses."
    Ko J., Kim S., Chung H.S., Kim K., Han K., Kim H., Jun H., Kaang B.-K., Kim E.
    Neuron 50:233-245(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LRFN1.

Entry informationi

Entry nameiDLGP1_RAT
AccessioniPrimary (citable) accession number: P97836
Secondary accession number(s): O54773, P97841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi