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Protein

Disks large-associated protein 1

Gene

Dlgap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the postsynaptic scaffold in neuronal cells.

GO - Molecular functioni

  • protein complex binding Source: RGD
  • protein domain specific binding Source: RGD

GO - Biological processi

  • aggresome assembly Source: BHF-UCL
  • chemical synaptic transmission Source: InterPro
  • protein localization to synapse Source: BHF-UCL
  • regulation of proteasomal protein catabolic process Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-6794361. Interactions of neurexins and neuroligins at synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 1
Short name:
DAP-1
Alternative name(s):
Guanylate kinase-associated protein
Short name:
rGKAP
PSD-95/SAP90-binding protein 1
SAP90/PSD-95-associated protein 1
Short name:
SAPAP1
Gene namesi
Name:Dlgap1
Synonyms:Gkap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi620223. Dlgap1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: RGD
  • synapse Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi990T → A: Abolishes interaction with SHANK1. 1 Publication1
Mutagenesisi991R → D: Abolishes interaction with SHANK1. 1 Publication1
Mutagenesisi992L → A: Abolishes interaction with SHANK1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001742901 – 992Disks large-associated protein 1Add BLAST992

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169PhosphoserineBy similarity1
Modified residuei362PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei368PhosphoserineBy similarity1
Modified residuei372PhosphoserineBy similarity1
Modified residuei389PhosphoserineCombined sources1
Modified residuei418PhosphoserineCombined sources1
Modified residuei421PhosphoserineCombined sources1
Modified residuei425PhosphoserineCombined sources1
Modified residuei428PhosphoserineCombined sources1
Modified residuei437PhosphoserineCombined sources1
Modified residuei509PhosphoserineCombined sources1
Modified residuei516PhosphoserineBy similarity1
Modified residuei578PhosphoserineBy similarity1
Modified residuei579PhosphothreonineBy similarity1
Modified residuei581PhosphoserineBy similarity1
Modified residuei605PhosphoserineCombined sources1
Modified residuei606PhosphothreonineBy similarity1
Modified residuei608PhosphoserineCombined sources1
Modified residuei611PhosphoserineCombined sources1
Modified residuei947PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97836.
PRIDEiP97836.

PTM databases

iPTMnetiP97836.
PhosphoSitePlusiP97836.

Expressioni

Tissue specificityi

Expressed in brain and testis.

Developmental stagei

Highest level of isoform 1 in the brain of newborn rats. Increasing levels of isoforms 2, 3, 4, and 5 in the brain of newborn rats from birth to 6 weeks of postnatal development. Increasing but low level of isoform 6 is expressed in the brain from 2 to 6 weeks of postnatal development.1 Publication

Gene expression databases

ExpressionAtlasiP97836. baseline and differential.

Interactioni

Subunit structurei

Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DLG4P783523EBI-6269434,EBI-80389From a different organism.
Dlg4P310169EBI-80901,EBI-375655
Magi2O883823EBI-80901,EBI-696179
Shank1Q9WV486EBI-80901,EBI-80909

GO - Molecular functioni

  • protein complex binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi249233. 7 interactors.
IntActiP97836. 15 interactors.
MINTiMINT-101502.
STRINGi10116.ENSRNOP00000022351.

Structurei

Secondary structure

1992
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi808 – 835Combined sources28
Helixi841 – 858Combined sources18
Helixi860 – 871Combined sources12
Helixi882 – 907Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4R0YX-ray2.00A/B807-916[»]
A/B946-971[»]
SMRiP97836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni665 – 676Interaction with DYL21 PublicationAdd BLAST12
Regioni687 – 698Interaction with DYL21 PublicationAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi990 – 992PDZ-binding3

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
GeneTreeiENSGT00550000074473.
HOGENOMiHOG000015346.
HOVERGENiHBG018957.
InParanoidiP97836.
KOiK15008.
PhylomeDBiP97836.

Family and domain databases

InterProiIPR030524. DLGAP1.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF7. PTHR12353:SF7. 2 hits.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97836-1) [UniParc]FASTAAdd to basket
Also known as: SAPAP1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGLSGSRSH HHGITCESAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR
60 70 80 90 100
NSFQAECVGP FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP
110 120 130 140 150
TNLLDQFERQ LPLSRDGYHT LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT
160 170 180 190 200
KSHSLEGASK GGVNGGKASP DGSQTVRYGK RSKSKERRSE PKARSNASNA
210 220 230 240 250
SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSVSQ YFMGAYNTIS
260 270 280 290 300
EQAVKASRSN NDVKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK
310 320 330 340 350
ASVNMDQAVV KSEACQQERS CQYLQVPQDE WTGYTPRGKD DEIPCRRMRS
360 370 380 390 400
GSYIKAMGDE DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN
410 420 430 440 450
EHSPKLQIRS HSYLRAVSEV SINRSLDSLD PAGLLTSPKF RSRNESYMRA
460 470 480 490 500
MSTISQVSEM EVNGQFESVC ESVFSELESQ AVEALDLPMP GCFRMRSHSY
510 520 530 540 550
VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK LSSAVEVSSC
560 570 580 590 600
ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS
610 620 630 640 650
GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV
660 670 680 690 700
TTEDRKKDFK KNRCLSIGIQ VDDAEESEKM AESKTSSKFQ SVGVQVEEEK
710 720 730 740 750
CFRRFTRSNS VTTAVQADLD FHDNLENSLE SIEDNSCPGP MARQFSRDAS
760 770 780 790 800
TSTVSIQGSG NHYHACAADD DFDTDFDPSI LPPPDPWIDS ITEDPLEAVQ
810 820 830 840 850
RSVCHRDGHW FLKLLQAERD RMEGWCKQME REERENNLPE DILGKIRTAV
860 870 880 890 900
GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS
910 920 930 940 950
MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES
960 970 980 990
SQRQEARKRL MAAKRAASVR QNSATESAES IEIYIPEAQT RL
Length:992
Mass (Da):110,178
Last modified:May 1, 1997 - v1
Checksum:i44BAF9BC0C14C099
GO
Isoform 2 (identifier: P97836-4) [UniParc]FASTAAdd to basket
Also known as: GKAP C, 2A

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW

Show »
Length:694
Mass (Da):77,263
Checksum:i2263D659AD1F6627
GO
Isoform 3 (identifier: P97836-5) [UniParc]FASTAAdd to basket
Also known as: GKAP A, GKAP1a

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
     547-574: Missing.

Show »
Length:666
Mass (Da):74,220
Checksum:iF027510C20BC2290
GO
Isoform 4 (identifier: P97836-6) [UniParc]FASTAAdd to basket
Also known as: GKAP B, 2C

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
     537-546: Missing.

Show »
Length:684
Mass (Da):76,279
Checksum:iC37FA0BA715BAF66
GO
Isoform 5 (identifier: P97836-7) [UniParc]FASTAAdd to basket
Also known as: 2B

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-324: QKASVNMDQAVVKSEACQQERSCQYL → MIDLFKAEWVSSVCVQVSRNGRTD

Show »
Length:692
Mass (Da):76,978
Checksum:i7180180258ADEF72
GO
Isoform 6 (identifier: P97836-8) [UniParc]FASTAAdd to basket
Also known as: 2D

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MNLIFHKDILFGVSATK

Show »
Length:684
Mass (Da):76,043
Checksum:i991D69B760948790
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti636S → T in AAC53054 (PubMed:9024696).Curated1
Sequence conflicti636S → T in BAA24265 (PubMed:9428732).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0154151 – 298Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6. 2 PublicationsAdd BLAST298
Alternative sequenceiVSP_015416299 – 325QKASV…CQYLQ → MIDLFKAEWVSSVCVQVSRN GRTDQVW in isoform 2, isoform 3 and isoform 4. 2 PublicationsAdd BLAST27
Alternative sequenceiVSP_015417299 – 325QKASV…CQYLQ → MNLIFHKDILFGVSATK in isoform 6. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_015418299 – 324QKASV…SCQYL → MIDLFKAEWVSSVCVQVSRN GRTD in isoform 5. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_015419537 – 546Missing in isoform 4. 2 Publications10
Alternative sequenceiVSP_015420547 – 574Missing in isoform 3. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67987 mRNA. Translation: AAC53054.1.
AB003594 mRNA. Translation: BAA24265.1.
U67137 mRNA. Translation: AAB48587.1.
PIRiT00025.
RefSeqiNP_001291216.1. NM_001304287.1. [P97836-7]
XP_008765652.1. XM_008767430.2. [P97836-4]
XP_008765654.1. XM_008767432.2. [P97836-8]
UniGeneiRn.214229.
Rn.90059.

Genome annotation databases

EnsembliENSRNOT00000087592; ENSRNOP00000072852; ENSRNOG00000016196. [P97836-7]
GeneIDi65040.
KEGGirno:65040.
UCSCiRGD:620223. rat. [P97836-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U67987 mRNA. Translation: AAC53054.1.
AB003594 mRNA. Translation: BAA24265.1.
U67137 mRNA. Translation: AAB48587.1.
PIRiT00025.
RefSeqiNP_001291216.1. NM_001304287.1. [P97836-7]
XP_008765652.1. XM_008767430.2. [P97836-4]
XP_008765654.1. XM_008767432.2. [P97836-8]
UniGeneiRn.214229.
Rn.90059.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4R0YX-ray2.00A/B807-916[»]
A/B946-971[»]
SMRiP97836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249233. 7 interactors.
IntActiP97836. 15 interactors.
MINTiMINT-101502.
STRINGi10116.ENSRNOP00000022351.

PTM databases

iPTMnetiP97836.
PhosphoSitePlusiP97836.

Proteomic databases

PaxDbiP97836.
PRIDEiP97836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000087592; ENSRNOP00000072852; ENSRNOG00000016196. [P97836-7]
GeneIDi65040.
KEGGirno:65040.
UCSCiRGD:620223. rat. [P97836-1]

Organism-specific databases

CTDi9229.
RGDi620223. Dlgap1.

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
GeneTreeiENSGT00550000074473.
HOGENOMiHOG000015346.
HOVERGENiHBG018957.
InParanoidiP97836.
KOiK15008.
PhylomeDBiP97836.

Enzyme and pathway databases

ReactomeiR-RNO-6794361. Interactions of neurexins and neuroligins at synapses.

Miscellaneous databases

PROiP97836.

Gene expression databases

ExpressionAtlasiP97836. baseline and differential.

Family and domain databases

InterProiIPR030524. DLGAP1.
IPR005026. SAPAP.
[Graphical view]
PANTHERiPTHR12353:SF7. PTHR12353:SF7. 2 hits.
PfamiPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDLGP1_RAT
AccessioniPrimary (citable) accession number: P97836
Secondary accession number(s): O54773, P97841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.