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P97836 (DLGP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disks large-associated protein 1

Short name=DAP-1
Alternative name(s):
Guanylate kinase-associated protein
Short name=rGKAP
PSD-95/SAP90-binding protein 1
SAP90/PSD-95-associated protein 1
Short name=SAPAP1
Gene names
Name:Dlgap1
Synonyms:Gkap
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length992 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the postsynaptic scaffold in neuronal cells.

Subunit structure

Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Cell membrane; Peripheral membrane protein. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell junctionsynapse.

Tissue specificity

Expressed in brain and testis.

Developmental stage

Highest level of isoform 1 in the brain of newborn rats. Increasing levels of isoforms 2, 3, 4, and 5 in the brain of newborn rats from birth to 6 weeks of postnatal development. Increasing but low level of isoform 6 is expressed in the brain from 2 to 6 weeks of postnatal development. Ref.2

Sequence similarities

Belongs to the SAPAP family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DLG4P783523EBI-6269434,EBI-80389From a different organism.
Dlg4P310169EBI-80901,EBI-375655
Magi2O883823EBI-80901,EBI-696179
Shank1Q9WV486EBI-80901,EBI-80909

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97836-1)

Also known as: SAPAP1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97836-4)

Also known as: GKAP C; 2A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
Isoform 3 (identifier: P97836-5)

Also known as: GKAP A; GKAP1a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
     547-574: Missing.
Isoform 4 (identifier: P97836-6)

Also known as: GKAP B; 2C;

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MIDLFKAEWVSSVCVQVSRNGRTDQVW
     537-546: Missing.
Isoform 5 (identifier: P97836-7)

Also known as: 2B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-324: QKASVNMDQAVVKSEACQQERSCQYL → MIDLFKAEWVSSVCVQVSRNGRTD
Isoform 6 (identifier: P97836-8)

Also known as: 2D;

The sequence of this isoform differs from the canonical sequence as follows:
     1-298: Missing.
     299-325: QKASVNMDQAVVKSEACQQERSCQYLQ → MNLIFHKDILFGVSATK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 992992Disks large-associated protein 1
PRO_0000174290

Regions

Region665 – 67612Interaction with DYL2
Region687 – 69812Interaction with DYL2
Motif990 – 9923PDZ-binding

Amino acid modifications

Modified residue3621Phosphoserine By similarity
Modified residue5091Phosphoserine By similarity
Modified residue5161Phosphoserine By similarity
Modified residue9471Phosphoserine By similarity

Natural variations

Alternative sequence1 – 298298Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6.
VSP_015415
Alternative sequence299 – 32527QKASV…CQYLQ → MIDLFKAEWVSSVCVQVSRN GRTDQVW in isoform 2, isoform 3 and isoform 4.
VSP_015416
Alternative sequence299 – 32527QKASV…CQYLQ → MNLIFHKDILFGVSATK in isoform 6.
VSP_015417
Alternative sequence299 – 32426QKASV…SCQYL → MIDLFKAEWVSSVCVQVSRN GRTD in isoform 5.
VSP_015418
Alternative sequence537 – 54610Missing in isoform 4.
VSP_015419
Alternative sequence547 – 57428Missing in isoform 3.
VSP_015420

Experimental info

Mutagenesis9901T → A: Abolishes interaction with SHANK1. Ref.7
Mutagenesis9911R → D: Abolishes interaction with SHANK1. Ref.7
Mutagenesis9921L → A: Abolishes interaction with SHANK1. Ref.7
Sequence conflict6361S → T in AAC53054. Ref.1
Sequence conflict6361S → T in BAA24265. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (SAPAP1) [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 44BAF9BC0C14C099

FASTA992110,178
        10         20         30         40         50         60 
MKGLSGSRSH HHGITCESAC DSLSHHSDHK PYLLSPVDHH PADHPYYTQR NSFQAECVGP 

        70         80         90        100        110        120 
FSDPLASSTF PRRHYTSQQE LKDESALVPR TLATKANRLP TNLLDQFERQ LPLSRDGYHT 

       130        140        150        160        170        180 
LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT KSHSLEGASK GGVNGGKASP DGSQTVRYGK 

       190        200        210        220        230        240 
RSKSKERRSE PKARSNASNA SPTSPSWWSS DDNLDGDMCL YHTPSGVMTM GRCPDRSVSQ 

       250        260        270        280        290        300 
YFMGAYNTIS EQAVKASRSN NDVKCSTCAN LPVTLDAPLL KKSAWSSTLT VSRAREVYQK 

       310        320        330        340        350        360 
ASVNMDQAVV KSEACQQERS CQYLQVPQDE WTGYTPRGKD DEIPCRRMRS GSYIKAMGDE 

       370        380        390        400        410        420 
DSGDSDTSPK PSPKVAARRE SYLKATQPSL TELTTLKISN EHSPKLQIRS HSYLRAVSEV 

       430        440        450        460        470        480 
SINRSLDSLD PAGLLTSPKF RSRNESYMRA MSTISQVSEM EVNGQFESVC ESVFSELESQ 

       490        500        510        520        530        540 
AVEALDLPMP GCFRMRSHSY VRAIEKGCSQ DDECVSLRSS SPPRTTTTVR TIQSSTGVIK 

       550        560        570        580        590        600 
LSSAVEVSSC ITTYKKTPPP VPPRTTTKPF ISITAQSSTE SAQDAYMDGQ GQRGDMISQS 

       610        620        630        640        650        660 
GLSNSTESLD SMKALTAAIE AANAQIHGPA SQHMGSNAAA VTTTTTIATV TTEDRKKDFK 

       670        680        690        700        710        720 
KNRCLSIGIQ VDDAEESEKM AESKTSSKFQ SVGVQVEEEK CFRRFTRSNS VTTAVQADLD 

       730        740        750        760        770        780 
FHDNLENSLE SIEDNSCPGP MARQFSRDAS TSTVSIQGSG NHYHACAADD DFDTDFDPSI 

       790        800        810        820        830        840 
LPPPDPWIDS ITEDPLEAVQ RSVCHRDGHW FLKLLQAERD RMEGWCKQME REERENNLPE 

       850        860        870        880        890        900 
DILGKIRTAV GSAQLLMAQK FYQFRELCEE NLNPNAHPRP TSQDLAGFWD MLQLSIENIS 

       910        920        930        940        950        960 
MKFDELHQLK ANNWKQMDPL DKKERRAPPP VPKKPAKGPA PLIRERSLES SQRQEARKRL 

       970        980        990 
MAAKRAASVR QNSATESAES IEIYIPEAQT RL 

« Hide

Isoform 2 (GKAP C) (2A) [UniParc].

Checksum: 2263D659AD1F6627
Show »

FASTA69477,263
Isoform 3 (GKAP A) (GKAP1a) [UniParc].

Checksum: F027510C20BC2290
Show »

FASTA66674,220
Isoform 4 (GKAP B) (2C) [UniParc].

Checksum: C37FA0BA715BAF66
Show »

FASTA68476,279
Isoform 5 (2B) [UniParc].

Checksum: 7180180258ADEF72
Show »

FASTA69276,978
Isoform 6 (2D) [UniParc].

Checksum: 991D69B760948790
Show »

FASTA68476,043

References

[1]"GKAP, a novel synaptic protein that interacts with the guanylate kinase-like domain of the PSD-95/SAP90 family of channel clustering molecules."
Kim E., Naisbitt S., Hsueh Y.-P., Rao A., Rothschild A., Craig A.M., Sheng M.
J. Cell Biol. 136:669-678(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), INTERACTION WITH DLG1; DLG2; DLG3 AND DLG4.
Tissue: Brain.
[2]"Differential expression of isoforms of PSD-95 binding protein (GKAP/SAPAP1) during rat brain development."
Kawashima N., Takamiya K., Sun J., Kitabatake A., Sobue K.
FEBS Lett. 418:301-304(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 4; 5 AND 6), DEVELOPMENTAL STAGE.
[3]"SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density."
Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y.
J. Biol. Chem. 272:11943-11951(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"BEGAIN (brain-enriched guanylate kinase-associated protein), a novel neuronal PSD-95/SAP90-binding protein."
Deguchi M., Hata Y., Takeuchi M., Ide N., Hirao K., Yao I., Irie M., Toyoda A., Takai Y.
J. Biol. Chem. 273:26269-26272(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BEGAIN AND DLG4.
[5]"Proline-rich synapse-associated proteins ProSAP1 and ProSAP2 interact with synaptic proteins of the SAPAP/GKAP family."
Boeckers T.M., Winter C., Smalla K.-H., Kreutz M.R., Bockmann J., Seidenbecher C., Garner C.C., Gundelfinger E.D.
Biochem. Biophys. Res. Commun. 264:247-252(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DLG4 AND SHANK PROTEINS.
[6]"Synamon, a novel neuronal protein interacting with synapse-associated protein 90/postsynaptic density-95-associated protein."
Yao I., Hata Y., Hirao K., Deguchi M., Ide N., Takeuchi M., Takai Y.
J. Biol. Chem. 274:27463-27466(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DLG4 AND SHANK1.
Tissue: Brain.
[7]"Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin."
Naisbitt S., Kim E., Tu J.C., Xiao B., Sala C., Valtschanoff J., Weinberg R.J., Worley P.F., Sheng M.
Neuron 23:569-582(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SHANK PROTEINS, MUTAGENESIS OF THR-990; ARG-991 AND LEU-992.
[8]"A novel multiple PDZ domain-containing molecule interacting with N-methyl-d-aspartate receptors and neuronal cell adhesion proteins."
Hirao K., Hata Y., Ide N., Takeuchi M., Irie M., Yao I., Deguchi M., Toyoda A., Suedhof T.C., Takai Y.
J. Biol. Chem. 273:21105-21110(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AIP1.
[9]"Proteomic identification of brain proteins that interact with dynein light chain LC8."
Navarro-Lerida I., Martinez Moreno M., Roncal F., Gavilanes F., Albar J.P., Rodriguez-Crespo I.
Proteomics 4:339-346(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYL2.
[10]"SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses."
Ko J., Kim S., Chung H.S., Kim K., Han K., Kim H., Jun H., Kaang B.-K., Kim E.
Neuron 50:233-245(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LRFN1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U67987 mRNA. Translation: AAC53054.1.
AB003594 mRNA. Translation: BAA24265.1.
U67137 mRNA. Translation: AAB48587.1.
PIRT00025.
RefSeqNP_075235.2. NM_022946.2.
UniGeneRn.90059.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249233. 7 interactions.
IntActP97836. 15 interactions.
MINTMINT-101502.
STRING10116.ENSRNOP00000022351.

PTM databases

PhosphoSiteP97836.

Proteomic databases

PaxDbP97836.
PRIDEP97836.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID65040.
KEGGrno:65040.
UCSCRGD:620223. rat. [P97836-1]

Organism-specific databases

CTD9229.
RGD620223. Dlgap1.

Phylogenomic databases

eggNOGNOG277836.
HOGENOMHOG000015346.
HOVERGENHBG018957.
InParanoidP97836.
KOK15008.
PhylomeDBP97836.

Gene expression databases

GenevestigatorP97836.

Family and domain databases

InterProIPR005026. GKAP.
[Graphical view]
PfamPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613856.
PROP97836.

Entry information

Entry nameDLGP1_RAT
AccessionPrimary (citable) accession number: P97836
Secondary accession number(s): O54773, P97841
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families