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Protein

Heart- and neural crest derivatives-expressed protein 1

Gene

Hand1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heart- and neural crest derivatives-expressed protein 1
Alternative name(s):
Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1
Short name:
eHAND
Gene namesi
Name:Hand1
Synonyms:Ehand
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621206. Hand1.

Subcellular locationi

  • Nucleusnucleoplasm By similarity
  • Nucleusnucleolus By similarity

  • Note: Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
  • RNA polymerase II transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Heart- and neural crest derivatives-expressed protein 1PRO_0000127187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei107 – 1071Phosphothreonine; by PLK4By similarity
Modified residuei109 – 1091Phosphoserine; by PLK4By similarity

Post-translational modificationi

Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97832.

Expressioni

Gene expression databases

GenevisibleiP97832. RN.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription factors). Interacts with MDFIC (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003486.

Structurei

3D structure databases

ProteinModelPortaliP97832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 14653bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 179Poly-His
Compositional biasi66 – 716Poly-Ala

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00760000119097.
HOGENOMiHOG000232082.
HOVERGENiHBG051880.
InParanoidiP97832.
KOiK09071.
OMAiRPYFQSW.
OrthoDBiEOG7G1V82.
PhylomeDBiP97832.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLVGSYAHH HHHHHSHPPH PMLHEPFLFG PASRCHQERP YFQSWLLSPA
60 70 80 90 100
DAAPDFPAGG PPPTTAVAAA AYGPDARPSQ SPGRLEALGG RLPRRKGSGP
110 120 130 140 150
KKERRRTESI NSAFAELREC IPNVPADTKL SKIKTLRLAT SYIAYLMDVL
160 170 180 190 200
AKDAQAGDPE AFKAELKKTD GGRESKRKRE SPQQHEGLPP ALGPSEKRIK
210
GRTGWPQQVW ALELNQ
Length:216
Mass (Da):23,810
Last modified:May 1, 1999 - v2
Checksum:iFD047598350C9BBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08140 mRNA. Translation: CAA69334.1.
BC097324 mRNA. Translation: AAH97324.1.
RefSeqiNP_067603.1. NM_021592.2.
UniGeneiRn.100787.
Rn.39340.

Genome annotation databases

EnsembliENSRNOT00000003486; ENSRNOP00000003486; ENSRNOG00000002582.
GeneIDi59112.
KEGGirno:59112.
UCSCiRGD:621206. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08140 mRNA. Translation: CAA69334.1.
BC097324 mRNA. Translation: AAH97324.1.
RefSeqiNP_067603.1. NM_021592.2.
UniGeneiRn.100787.
Rn.39340.

3D structure databases

ProteinModelPortaliP97832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003486.

Proteomic databases

PaxDbiP97832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003486; ENSRNOP00000003486; ENSRNOG00000002582.
GeneIDi59112.
KEGGirno:59112.
UCSCiRGD:621206. rat.

Organism-specific databases

CTDi9421.
RGDi621206. Hand1.

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00760000119097.
HOGENOMiHOG000232082.
HOVERGENiHBG051880.
InParanoidiP97832.
KOiK09071.
OMAiRPYFQSW.
OrthoDBiEOG7G1V82.
PhylomeDBiP97832.
TreeFamiTF315153.

Miscellaneous databases

NextBioi611755.
PROiP97832.

Gene expression databases

GenevisibleiP97832. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of helix-loop-helix proteins in vascular smooth muscle."
    Kemp P., Grainger D., Metcalfe J.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Aorta.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.

Entry informationi

Entry nameiHAND1_RAT
AccessioniPrimary (citable) accession number: P97832
Secondary accession number(s): Q4V8L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: May 11, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.