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Protein

Leukocyte surface antigen CD47

Gene

Cd47

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus.By similarity1 Publication

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • cell migration Source: RGD
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • positive regulation of phagocytosis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Integrin

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-RNO-202733. Cell surface interactions at the vascular wall.
R-RNO-216083. Integrin cell surface interactions.
R-RNO-391160. Signal regulatory protein (SIRP) family interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukocyte surface antigen CD47
Alternative name(s):
Integrin-associated protein
Short name:
IAP
CD_antigen: CD47
Gene namesi
Name:Cd47
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2308. Cd47.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 140122ExtracellularSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 17312CytoplasmicSequence analysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 20612ExtracellularSequence analysisAdd
BLAST
Transmembranei207 – 22721HelicalSequence analysisAdd
BLAST
Topological domaini228 – 23811CytoplasmicSequence analysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence analysisAdd
BLAST
Topological domaini260 – 2667ExtracellularSequence analysis
Transmembranei267 – 28721HelicalSequence analysisAdd
BLAST
Topological domaini288 – 30316CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 303285Leukocyte surface antigen CD47PRO_0000042209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acidCurated
Disulfide bondi33 ↔ 261PROSITE-ProRule annotation
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi41 ↔ 112PROSITE-ProRule annotation
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence analysis
Glycosylationi73 – 731N-linked (GlcNAc...)Sequence analysis
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence analysis
Modified residuei87 – 871PhosphoserineCombined sources
Modified residuei89 – 891PhosphoserineCombined sources
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence analysis
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence analysis
Isoform 2 (identifier: P97829-2)
Modified residuei311 – 3111PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP97829.
PRIDEiP97829.

PTM databases

iPTMnetiP97829.
SwissPalmiP97829.
UniCarbKBiP97829.

Expressioni

Tissue specificityi

Expressed in hippocampus.1 Publication

Inductioni

By ferric nitrilotriacetate, NMDA and amphetamine.2 Publications

Gene expression databases

ExpressionAtlasiP97829. baseline and differential.
GenevisibleiP97829. RN.

Interactioni

Subunit structurei

Monomer. Interacts with fibrinogen, PTPNS1, SIRPG, THBS1, UBQLN1 and UBQLN2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248018. 1 interaction.
STRINGi10116.ENSRNOP00000050938.

Structurei

3D structure databases

ProteinModelPortaliP97829.
SMRiP97829. Positions 19-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 125107Ig-like V-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJTS. Eukaryota.
ENOG41113GI. LUCA.
GeneTreeiENSGT00390000007697.
HOGENOMiHOG000013020.
HOVERGENiHBG003808.
InParanoidiP97829.
KOiK06266.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97829-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWPLAAALLL GSCCCGSAQL LLSKVKSVEF TSCNDTVVIP CKVLNVEAQS
60 70 80 90 100
TDEMFVKWKL NKSYIFIYDG NKNSTTREQN FTSAKISVSD LLKGIASLTM
110 120 130 140 150
DTHEAVVGNY TCEVTELSRE GKTVIELKNR PVSWFSTNEK ILIVIFPILA
160 170 180 190 200
ILLFWGKFGI LTLKYKSSHT NKRIILLLVA GLALTLIVVV GAILFIPGEK
210 220 230 240 250
PVKNASGLGL IVISTGILIL LQYNVFMTAF GMTSFTIAIL ITQVLGYVLA
260 270 280 290 300
VVGMCLCIMA CEPVHGPLLI SGLGIIALAE LLGLVYMKFV ASNQRTIQPP

RNN
Length:303
Mass (Da):32,995
Last modified:May 1, 1997 - v1
Checksum:iE849397AB0DA8D65
GO
Isoform 2 (identifier: P97829-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     8-11: LLLG → R
     302-303: NN → KAVEEPLNAFKESKGMMNDE

Show »
Length:318
Mass (Da):34,776
Checksum:i3DAA570A280C3956
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei8 – 114LLLG → R in isoform 2. 1 PublicationVSP_015794
Alternative sequencei302 – 3032NN → KAVEEPLNAFKESKGMMNDE in isoform 2. 1 PublicationVSP_015795

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87659 mRNA. Translation: BAA13420.1.
AF017437 mRNA. Translation: AAB70273.1.
BC085740 mRNA. Translation: AAH85740.1.
RefSeqiNP_062068.1. NM_019195.2. [P97829-1]
UniGeneiRn.7409.

Genome annotation databases

EnsembliENSRNOT00000050142; ENSRNOP00000050938; ENSRNOG00000001964. [P97829-1]
ENSRNOT00000078434; ENSRNOP00000071430; ENSRNOG00000001964. [P97829-1]
GeneIDi29364.
KEGGirno:29364.
UCSCiRGD:2308. rat. [P97829-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87659 mRNA. Translation: BAA13420.1.
AF017437 mRNA. Translation: AAB70273.1.
BC085740 mRNA. Translation: AAH85740.1.
RefSeqiNP_062068.1. NM_019195.2. [P97829-1]
UniGeneiRn.7409.

3D structure databases

ProteinModelPortaliP97829.
SMRiP97829. Positions 19-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248018. 1 interaction.
STRINGi10116.ENSRNOP00000050938.

PTM databases

iPTMnetiP97829.
SwissPalmiP97829.
UniCarbKBiP97829.

Proteomic databases

PaxDbiP97829.
PRIDEiP97829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000050142; ENSRNOP00000050938; ENSRNOG00000001964. [P97829-1]
ENSRNOT00000078434; ENSRNOP00000071430; ENSRNOG00000001964. [P97829-1]
GeneIDi29364.
KEGGirno:29364.
UCSCiRGD:2308. rat. [P97829-1]

Organism-specific databases

CTDi961.
RGDi2308. Cd47.

Phylogenomic databases

eggNOGiENOG410IJTS. Eukaryota.
ENOG41113GI. LUCA.
GeneTreeiENSGT00390000007697.
HOGENOMiHOG000013020.
HOVERGENiHBG003808.
InParanoidiP97829.
KOiK06266.

Enzyme and pathway databases

ReactomeiR-RNO-202733. Cell surface interactions at the vascular wall.
R-RNO-216083. Integrin cell surface interactions.
R-RNO-391160. Signal regulatory protein (SIRP) family interactions.

Miscellaneous databases

PROiP97829.

Gene expression databases

ExpressionAtlasiP97829. baseline and differential.
GenevisibleiP97829. RN.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Overexpression of integrin-associated protein (CD47) in rat kidney treated with a renal carcinogen, ferric nitrilotriacetate."
    Nishiyama Y., Tanaka T., Naitoh H., Mori C., Fukumoto M., Hiai H., Toyokuni S.
    Jpn. J. Cancer Res. 88:120-128(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION.
    Strain: Wistar.
    Tissue: Kidney.
  2. "Expression of integrin-associated protein gene associated with memory formation in rats."
    Huang A.-M., Wang H.L., Tang Y.P., Lee E.H.Y.
    J. Neurosci. 18:4305-4313(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Brown Norway.
    Tissue: Heart.
  4. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 43-57; 63-93; 123-128 AND 158-164, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-89, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCD47_RAT
AccessioniPrimary (citable) accession number: P97829
Secondary accession number(s): O35294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: May 1, 1997
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.