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Protein

Acyl-protein thioesterase 1

Gene

Lypla1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity (By similarity).By similarity1 Publication

Catalytic activityi

Palmitoyl-protein + H2O = palmitate + protein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119Charge relay system1
Active sitei174Charge relay system1
Active sitei208Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • fatty acid metabolic process Source: UniProtKB-KW
  • negative regulation of Golgi to plasma membrane protein transport Source: MGI
  • protein depalmitoylation Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-203615 eNOS activation

Protein family/group databases

ESTHERimouse-lypla1 LYsophospholipase_carboxylesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-protein thioesterase 1 (EC:3.1.2.-)
Short name:
APT-1
Alternative name(s):
Lysophospholipase 1
Lysophospholipase I
Short name:
LPL-I
Short name:
LysoPLA I
Gene namesi
Name:Lypla1
Synonyms:Apt1, Pla1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1344588 Lypla1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi119S → A: Abolishes lysophospholipase activity. 1 Publication1
Mutagenesisi174D → A: Abolishes lysophospholipase activity. 1 Publication1
Mutagenesisi208H → A: Abolishes lysophospholipase activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3259479

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022681 – 230Acyl-protein thioesterase 1Add BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP97823
MaxQBiP97823
PaxDbiP97823
PeptideAtlasiP97823
PRIDEiP97823

PTM databases

iPTMnetiP97823
PhosphoSitePlusiP97823
SwissPalmiP97823

Expressioni

Gene expression databases

BgeeiENSMUSG00000025903
CleanExiMM_LYPLA1
MM_PLA1A
ExpressionAtlasiP97823 baseline and differential
GenevisibleiP97823 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi202216, 2 interactors
IntActiP97823, 4 interactors
MINTiP97823
STRINGi10090.ENSMUSP00000027036

Structurei

3D structure databases

ProteinModelPortaliP97823
SMRiP97823
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2112 Eukaryota
COG0400 LUCA
GeneTreeiENSGT00390000009648
HOGENOMiHOG000260139
HOVERGENiHBG052378
InParanoidiP97823
KOiK06128
OMAiCPHAPVR
OrthoDBiEOG091G080L
PhylomeDBiP97823
TreeFamiTF314619

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR003140 PLipase/COase/thioEstase
PfamiView protein in Pfam
PF02230 Abhydrolase_2, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97823-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK
60 70 80 90 100
YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI
110 120 130 140 150
DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA
160 170 180 190 200
SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF
210 220 230
KIYEGMMHSS CQQEMMDVKH FIDKLLPPID
Length:230
Mass (Da):24,688
Last modified:May 1, 1997 - v1
Checksum:i89AF2017AEFC9FAC
GO
Isoform 2 (identifier: P97823-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     214-230: EMMDVKHFIDKLLPPID → VGVSGSSE

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:221
Mass (Da):23,367
Checksum:i456BF0DCF6EE0F30
GO

Sequence cautioni

The sequence BAC34318 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156P → L in AAH52848 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009197214 – 230EMMDV…LPPID → VGVSGSSE in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89352 mRNA Translation: AAB48627.1
AK002674 mRNA Translation: BAB22276.1
AK050549 mRNA Translation: BAC34318.1 Different initiation.
AK146874 mRNA Translation: BAE27497.1
AK167231 mRNA Translation: BAE39355.1
BC013536 mRNA Translation: AAH13536.1
BC052848 mRNA Translation: AAH52848.1
CCDSiCCDS14806.1 [P97823-1]
RefSeqiNP_032892.1, NM_008866.2 [P97823-1]
UniGeneiMm.299955

Genome annotation databases

EnsembliENSMUST00000027036; ENSMUSP00000027036; ENSMUSG00000025903 [P97823-1]
ENSMUST00000150971; ENSMUSP00000137248; ENSMUSG00000025903 [P97823-2]
GeneIDi18777
KEGGimmu:18777
UCSCiuc007afh.1 mouse [P97823-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiLYPA1_MOUSE
AccessioniPrimary (citable) accession number: P97823
Secondary accession number(s): Q3TJZ0, Q7TPX1, Q8BWM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health