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Protein

Acidic leucine-rich nuclear phosphoprotein 32 family member E

Gene

Anp32e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.4 Publications

GO - Molecular functioni

  • histone binding Source: UniProtKB
  • phosphatase inhibitor activity Source: MGI

GO - Biological processi

  • histone exchange Source: UniProtKB
  • negative regulation of catalytic activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Chromatin regulator

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic leucine-rich nuclear phosphoprotein 32 family member E
Alternative name(s):
Cerebellar postnatal development protein 1
LANP-like protein
Short name:
LANP-L
Gene namesi
Name:Anp32e
Synonyms:Cpd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1913721. Anp32e.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: MGI
  • nucleus Source: MGI
  • Swr1 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice are viable and fertile (PubMed:20844742, PubMed:21049064, PubMed:23675506). They display a subtle neurological clasping phenotype and mild motor deficits (PubMed:20844742). Motor defects were not confirmed by a subsequent analysis (PubMed:23675506). Deletion in embryonic fibroblasts results in the appearance of a significant number of new H2A.Z/H2AFZ around the transcription start site as well as at other chromatin regions (PubMed:24463511).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Acidic leucine-rich nuclear phosphoprotein 32 family member EPRO_0000137600Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP97822.
MaxQBiP97822.
PaxDbiP97822.
PRIDEiP97822.

PTM databases

iPTMnetiP97822.
PhosphoSiteiP97822.

Expressioni

Tissue specificityi

Expressed at highest levels in cerebellum and spleen. In the cerebellum, expressed mainly in granule cells and, to a lesser extent, in Purkinje cells.1 Publication

Developmental stagei

Low levels are found at postnatal day 4. Levels increase from postnatal day 7 to postnatal day 17. Levels decrease and remain low in the adult.1 Publication

Gene expression databases

BgeeiP97822.
ExpressionAtlasiP97822. baseline and differential.
GenevisibleiP97822. MM.

Interactioni

Subunit structurei

Component of a SWR1-like complex, composed of EP400, KAT5/TIP60, TRRAP, BRD8, RUVBL1, RUVBL2, ING3 and ANP32E; the complex does not contain SRCAP. Interacts with H2A.Z/H2AFZ (By similarity). Interacts with the importin alpha KPNA1 and KPNA2.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211498. 2 interactions.
IntActiP97822. 1 interaction.
MINTiMINT-4087684.
STRINGi10090.ENSMUSP00000128483.

Structurei

3D structure databases

ProteinModelPortaliP97822.
SMRiP97822. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 3821LRR 1Add
BLAST
Repeati43 – 6422LRR 2Add
BLAST
Repeati65 – 8723LRR 3Add
BLAST
Repeati89 – 11022LRR 4Add
BLAST
Domaini123 – 16139LRRCTAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni207 – 26054ZID domainBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi151 – 260110Asp/Glu-rich (highly acidic)Add
BLAST

Domaini

The H2A.Z-interacting domain (ZID) mediates a direct interaction with H2A.Z/H2AFZ.By similarity

Sequence similaritiesi

Belongs to the ANP32 family.Curated
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG2739. Eukaryota.
ENOG4111HZT. LUCA.
GeneTreeiENSGT00560000077130.
HOGENOMiHOG000007361.
HOVERGENiHBG053102.
InParanoidiP97822.
KOiK18648.
OMAiRCPNLTY.
OrthoDBiEOG7TJ3KH.
PhylomeDBiP97822.
TreeFamiTF317206.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97822-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMKKKINME LKNRAPEEVT ELVLDNCLCV NGEIEGLNDT FKELEFLSMA
60 70 80 90 100
NVELSSLARL PSLNKLRKLE LSDNIISGGL EVLAEKCPNL TYLNLSGNKI
110 120 130 140 150
KDLSTVEALQ NLKNLKSLDL FNCEITNLED YRESIFELLQ QITYLDGFDQ
160 170 180 190 200
EDNEAPDSEE EDDDDEDGDE DEEDEDEDEA GPPEGYEEEE DDDEDEAGSE
210 220 230 240 250
VGEGEEEVGL SYLMKDEIQD EEDDDDYVDE GEEEEEEEEE GLRGEKRKRD
260
AEDDGEEDDD
Length:260
Mass (Da):29,622
Last modified:May 1, 2000 - v2
Checksum:i7F94E46D72A04780
GO
Isoform 2 (identifier: P97822-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     208-219: Missing.

Note: No experimental confirmation available.
Show »
Length:248
Mass (Da):28,245
Checksum:iA4359037032D77BA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981N → D in BAB28449 (PubMed:16141072).Curated
Sequence conflicti199 – 1991S → T in BAC33858 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei208 – 21912Missing in isoform 2. 1 PublicationVSP_007373Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89345 mRNA. Translation: AAB49462.2.
AB037685 mRNA. Translation: BAB03507.1.
AK012759 mRNA. Translation: BAB28449.1.
AK049647 mRNA. Translation: BAC33858.1.
AK076049 mRNA. Translation: BAC36147.1.
AK088401 mRNA. Translation: BAC40331.1.
AK147888 mRNA. Translation: BAE28205.1.
AK168380 mRNA. Translation: BAE40309.1.
BC005690 mRNA. Translation: AAH05690.1.
BC080684 mRNA. Translation: AAH80684.1.
CCDSiCCDS17626.1. [P97822-1]
CCDS57238.1. [P97822-2]
RefSeqiNP_001240686.1. NM_001253757.1. [P97822-2]
NP_075699.3. NM_023210.4. [P97822-1]
UniGeneiMm.218657.

Genome annotation databases

EnsembliENSMUST00000015893; ENSMUSP00000015893; ENSMUSG00000015749. [P97822-2]
ENSMUST00000165307; ENSMUSP00000128483; ENSMUSG00000015749. [P97822-1]
GeneIDi66471.
KEGGimmu:66471.
UCSCiuc008qlu.2. mouse. [P97822-1]
uc008qlv.2. mouse. [P97822-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89345 mRNA. Translation: AAB49462.2.
AB037685 mRNA. Translation: BAB03507.1.
AK012759 mRNA. Translation: BAB28449.1.
AK049647 mRNA. Translation: BAC33858.1.
AK076049 mRNA. Translation: BAC36147.1.
AK088401 mRNA. Translation: BAC40331.1.
AK147888 mRNA. Translation: BAE28205.1.
AK168380 mRNA. Translation: BAE40309.1.
BC005690 mRNA. Translation: AAH05690.1.
BC080684 mRNA. Translation: AAH80684.1.
CCDSiCCDS17626.1. [P97822-1]
CCDS57238.1. [P97822-2]
RefSeqiNP_001240686.1. NM_001253757.1. [P97822-2]
NP_075699.3. NM_023210.4. [P97822-1]
UniGeneiMm.218657.

3D structure databases

ProteinModelPortaliP97822.
SMRiP97822. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211498. 2 interactions.
IntActiP97822. 1 interaction.
MINTiMINT-4087684.
STRINGi10090.ENSMUSP00000128483.

PTM databases

iPTMnetiP97822.
PhosphoSiteiP97822.

Proteomic databases

EPDiP97822.
MaxQBiP97822.
PaxDbiP97822.
PRIDEiP97822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015893; ENSMUSP00000015893; ENSMUSG00000015749. [P97822-2]
ENSMUST00000165307; ENSMUSP00000128483; ENSMUSG00000015749. [P97822-1]
GeneIDi66471.
KEGGimmu:66471.
UCSCiuc008qlu.2. mouse. [P97822-1]
uc008qlv.2. mouse. [P97822-2]

Organism-specific databases

CTDi81611.
MGIiMGI:1913721. Anp32e.

Phylogenomic databases

eggNOGiKOG2739. Eukaryota.
ENOG4111HZT. LUCA.
GeneTreeiENSGT00560000077130.
HOGENOMiHOG000007361.
HOVERGENiHBG053102.
InParanoidiP97822.
KOiK18648.
OMAiRCPNLTY.
OrthoDBiEOG7TJ3KH.
PhylomeDBiP97822.
TreeFamiTF317206.

Miscellaneous databases

ChiTaRSiAnp32e. mouse.
PROiP97822.
SOURCEiSearch...

Gene expression databases

BgeeiP97822.
ExpressionAtlasiP97822. baseline and differential.
GenevisibleiP97822. MM.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  2. "Characterization of the nuclear transport of a novel leucine-rich acidic nuclear protein-like protein."
    Matsubae M., Kurihara T., Tachibana T., Imamoto N., Yoneda Y.
    FEBS Lett. 468:171-175(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH KPNA1 AND KPNA2.
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Embryo, Kidney, Spinal cord and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Mammary gland.
  5. "Anp32e (Cpd1) and related protein phosphatase 2 inhibitors."
    Santa-Coloma T.A.
    Cerebellum 2:310-320(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Anp32e/Cpd1 regulates protein phosphatase 2A activity at synapses during synaptogenesis."
    Costanzo R.V., Vila-Ortiz G.J., Perandones C., Carminatti H., Matilla A., Radrizzani M.
    Eur. J. Neurosci. 23:309-324(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "The Anp32 family of proteins containing leucine-rich repeats."
    Matilla A., Radrizzani M.
    Cerebellum 4:7-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  9. "Cpd-1 null mice display a subtle neurological phenotype."
    Kular R.K., Gogliotti R.G., Opal P.
    PLoS ONE 5:0-0(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  10. Cited for: DISRUPTION PHENOTYPE.
  11. "Targeted ANP32E mutant mice do not demonstrate obvious movement defects."
    Wong P., Leo V.I., Low M., Mak T.W., Zhang X., Reilly P.T.
    PLoS ONE 8:E63815-E63815(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  12. Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiAN32E_MOUSE
AccessioniPrimary (citable) accession number: P97822
Secondary accession number(s): Q3TH89
, Q8BPF8, Q8C2L4, Q8C7Q8, Q9CZD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.