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Protein

Dipeptidyl peptidase 1

Gene

Ctsc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol protease. Has dipeptidylpeptidase activity. Can act as both an exopeptidase and endopeptidase. Can degrade glucagon. Plays a role in the generation of cytotoxic lymphocyte effector function.

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, except when Xaa is Arg or Lys, or Yaa or Zaa is Pro.

Cofactori

chlorideBy similarityNote: Binds 1 Cl- ion per heavy chain.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei257By similarity1
Binding sitei301ChlorideBy similarity1
Binding sitei303Chloride; via amide nitrogenBy similarity1
Binding sitei346ChlorideBy similarity1
Active sitei404By similarity1
Active sitei426By similarity1

GO - Molecular functioni

  • chloride ion binding Source: Ensembl
  • cysteine-type endopeptidase activity Source: GO_Central
  • cysteine-type peptidase activity Source: MGI
  • peptidase activator activity involved in apoptotic process Source: MGI
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • aging Source: Ensembl
  • positive regulation of apoptotic signaling pathway Source: MGI
  • positive regulation of proteolysis involved in cellular protein catabolic process Source: Ensembl
  • proteolysis Source: UniProtKB
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • response to organic substance Source: Ensembl
  • T cell mediated cytotoxicity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Chloride

Enzyme and pathway databases

BRENDAi3.4.14.1. 3474.
ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiC01.070.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidyl peptidase 1 (EC:3.4.14.1)
Alternative name(s):
Cathepsin C
Cathepsin J
Dipeptidyl peptidase I
Short name:
DPP-I
Short name:
DPPI
Dipeptidyl transferase
Cleaved into the following 3 chains:
Alternative name(s):
Dipeptidyl peptidase I exclusion domain chain
Alternative name(s):
Dipeptidyl peptidase I heavy chain
Alternative name(s):
Dipeptidyl peptidase I light chain
Gene namesi
Name:Ctsc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109553. Ctsc.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3454.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000002634625 – 134Dipeptidyl peptidase 1 exclusion domain chainBy similarityAdd BLAST110
PropeptideiPRO_0000026347135 – 230By similarityAdd BLAST96
ChainiPRO_0000026348231 – 393Dipeptidyl peptidase 1 heavy chainAdd BLAST163
ChainiPRO_0000026349394 – 462Dipeptidyl peptidase 1 light chainAdd BLAST69

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi30 ↔ 118By similarity
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi54 ↔ 136By similarity
Disulfide bondi254 ↔ 297By similarity
Glycosylationi275N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi290 ↔ 330By similarity
Disulfide bondi320 ↔ 336By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP97821.
PaxDbiP97821.
PeptideAtlasiP97821.
PRIDEiP97821.

PTM databases

iPTMnetiP97821.
PhosphoSitePlusiP97821.
SwissPalmiP97821.

Expressioni

Tissue specificityi

Broadly distributed, but higher levels found in lung, liver, kidney and spleen. Lower levels found in testis and brain.

Gene expression databases

BgeeiENSMUSG00000030560.
CleanExiMM_CTSC.
ExpressionAtlasiP97821. baseline and differential.
GenevisibleiP97821. MM.

Interactioni

Subunit structurei

Tetramer of heterotrimers consisting of exclusion domain, heavy- and light chains.By similarity

Protein-protein interaction databases

IntActiP97821. 1 interactor.
STRINGi10090.ENSMUSP00000032779.

Chemistry databases

BindingDBiP97821.

Structurei

3D structure databases

ProteinModelPortaliP97821.
SMRiP97821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000068022.
HOVERGENiHBG005248.
InParanoidiP97821.
KOiK01275.
OMAiYDDFLHY.
OrthoDBiEOG091G06TT.
PhylomeDBiP97821.
TreeFamiTF313225.

Family and domain databases

Gene3Di2.40.128.80. 1 hit.
InterProiIPR014882. CathepsinC_exc.
IPR033161. DPP_I.
IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PTHR12411:SF354. PTHR12411:SF354. 1 hit.
PfamiPF08773. CathepsinC_exc. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00645. Pept_C1. 1 hit.
[Graphical view]
SUPFAMiSSF75001. SSF75001. 1 hit.
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPWTHSLRA VLLLVLLGVC TVRSDTPANC TYPDLLGTWV FQVGPRSSRS
60 70 80 90 100
DINCSVMEAT EEKVVVHLKK LDTAYDELGN SGHFTLIYNQ GFEIVLNDYK
110 120 130 140 150
WFAFFKYEVR GHTAISYCHE TMTGWVHDVL GRNWACFVGK KVESHIEKVN
160 170 180 190 200
MNAAHLGGLQ ERYSERLYTH NHNFVKAINT VQKSWTATAY KEYEKMSLRD
210 220 230 240 250
LIRRSGHSQR IPRPKPAPMT DEIQQQILNL PESWDWRNVQ GVNYVSPVRN
260 270 280 290 300
QESCGSCYSF ASMGMLEARI RILTNNSQTP ILSPQEVVSC SPYAQGCDGG
310 320 330 340 350
FPYLIAGKYA QDFGVVEESC FPYTAKDSPC KPRENCLRYY SSDYYYVGGF
360 370 380 390 400
YGGCNEALMK LELVKHGPMA VAFEVHDDFL HYHSGIYHHT GLSDPFNPFE
410 420 430 440 450
LTNHAVLLVG YGRDPVTGIE YWIIKNSWGS NWGESGYFRI RRGTDECAIE
460
SIAVAAIPIP KL
Length:462
Mass (Da):52,376
Last modified:May 1, 1997 - v1
Checksum:i56574B38D7DF4710
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89269 mRNA. Translation: AAB49457.1.
U74683 mRNA. Translation: AAB58400.3.
BC067063 mRNA. Translation: AAH67063.1.
CCDSiCCDS21438.1.
RefSeqiNP_034112.3. NM_009982.5.
UniGeneiMm.322945.
Mm.483300.
Mm.89295.

Genome annotation databases

EnsembliENSMUST00000032779; ENSMUSP00000032779; ENSMUSG00000030560.
GeneIDi13032.
KEGGimmu:13032.
UCSCiuc009ifu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89269 mRNA. Translation: AAB49457.1.
U74683 mRNA. Translation: AAB58400.3.
BC067063 mRNA. Translation: AAH67063.1.
CCDSiCCDS21438.1.
RefSeqiNP_034112.3. NM_009982.5.
UniGeneiMm.322945.
Mm.483300.
Mm.89295.

3D structure databases

ProteinModelPortaliP97821.
SMRiP97821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97821. 1 interactor.
STRINGi10090.ENSMUSP00000032779.

Chemistry databases

BindingDBiP97821.
ChEMBLiCHEMBL3454.

Protein family/group databases

MEROPSiC01.070.

PTM databases

iPTMnetiP97821.
PhosphoSitePlusiP97821.
SwissPalmiP97821.

Proteomic databases

EPDiP97821.
PaxDbiP97821.
PeptideAtlasiP97821.
PRIDEiP97821.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032779; ENSMUSP00000032779; ENSMUSG00000030560.
GeneIDi13032.
KEGGimmu:13032.
UCSCiuc009ifu.2. mouse.

Organism-specific databases

CTDi1075.
MGIiMGI:109553. Ctsc.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000068022.
HOVERGENiHBG005248.
InParanoidiP97821.
KOiK01275.
OMAiYDDFLHY.
OrthoDBiEOG091G06TT.
PhylomeDBiP97821.
TreeFamiTF313225.

Enzyme and pathway databases

BRENDAi3.4.14.1. 3474.
ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiCtsc. mouse.
PROiP97821.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030560.
CleanExiMM_CTSC.
ExpressionAtlasiP97821. baseline and differential.
GenevisibleiP97821. MM.

Family and domain databases

Gene3Di2.40.128.80. 1 hit.
InterProiIPR014882. CathepsinC_exc.
IPR033161. DPP_I.
IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PTHR12411:SF354. PTHR12411:SF354. 1 hit.
PfamiPF08773. CathepsinC_exc. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00645. Pept_C1. 1 hit.
[Graphical view]
SUPFAMiSSF75001. SSF75001. 1 hit.
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATC_MOUSE
AccessioniPrimary (citable) accession number: P97821
Secondary accession number(s): O08853
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.