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P97814

- PPIP1_MOUSE

UniProt

P97814 - PPIP1_MOUSE

Protein

Proline-serine-threonine phosphatase-interacting protein 1

Gene

Pstpip1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation. Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.5 Publications

    GO - Molecular functioni

    1. actin binding Source: MGI
    2. protein binding Source: IntAct
    3. protein phosphatase binding Source: MGI

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. endocytosis Source: UniProtKB-KW
    3. inflammatory response Source: UniProtKB-KW
    4. innate immune response Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion, Endocytosis, Immunity, Inflammatory response, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline-serine-threonine phosphatase-interacting protein 1
    Short name:
    PEST phosphatase-interacting protein 1
    Gene namesi
    Name:Pstpip1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1321396. Pstpip1.

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeleton. Cell projectionlamellipodium. Cell projectionuropodium By similarity. Cytoplasmperinuclear region. Cleavage furrow
    Note: During interphase, colocalizes with F-actin in the cortical cytoskeleton, lamellipodia, and stress fibers. In dividing cells, colocalizes with the F-actin rich cytokinetic cleavage furrow. Colocalized with WAS to filamentous structures within the cytoplasm. Colocalized with PTPN12 in the cytoplasm and the perinuclear region. Colocalized with CD2AP and WAS in the actin cytoskeleton. Colocalized with CD2, CD2AP and WAS at the site of T-cell:APC contact. In monocytes, forms a branched filamentous network in the cytoplasm By similarity. In migrating neutrophils, localizes most strongly to the trailing edge of the uropod where it colocalizes with PIP5K1C By similarity. In transfected cells, forms relatively straight filaments radiating out from the nucleus By similarity. Colocalizes with MEFV, particularly at the branch point of filaments By similarity. Filament formation requires an intact tubulin cytoskeleton By similarity.By similarity

    GO - Cellular componenti

    1. actomyosin contractile ring Source: MGI
    2. cleavage furrow Source: MGI
    3. lamellipodium Source: UniProtKB-SubCell
    4. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    5. stress fiber Source: MGI
    6. uropod Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi344 – 3441Y → F: Complete loss of protein phosphorylation. 3 Publications
    Mutagenesisi367 – 3671Y → F: No effect on phosphorylation. 3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415Proline-serine-threonine phosphatase-interacting protein 1PRO_0000058540Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei344 – 3441Phosphotyrosine; by ABL11 Publication

    Post-translational modificationi

    Dephosphorylated on Tyr-344 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-344 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP97814.
    PRIDEiP97814.

    PTM databases

    PhosphoSiteiP97814.

    Expressioni

    Tissue specificityi

    Highly expressed in adult lung and spleen, and weakly expressed in testis, muscle, kidney, brain and heart. Highly expressed in spleen and thymus, moderately in lung, brain and muscle, and weakly expressed in heart and liver (at protein level).2 Publications

    Developmental stagei

    Highly expressed in the day 7 embryo (E7).1 Publication

    Gene expression databases

    BgeeiP97814.
    GenevestigatoriP97814.

    Interactioni

    Subunit structurei

    Homodimer. Homotrimer. Interacts (via coiled-coil domain) with CD2AP, PTPN12 and PTPN18. Interacts (via SH3 domain) with ABL1 and WAS. Interacts (via SH3 and coiled-coil domains) with MEFV (via B-box zinc finger); the interaction allows binding of MEFV to PYCARD and facilitates formation of PYCARD pyroptosomes. Interacts with DNM2 and FASLG By similarity. Interacts with CD2.By similarity5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Abl1P005205EBI-7484574,EBI-914519
    Ptpn12P358315EBI-7484574,EBI-2642957
    Ptpn18Q611523EBI-7484574,EBI-7074223

    Protein-protein interaction databases

    BioGridi202439. 2 interactions.
    IntActiP97814. 6 interactions.
    MINTiMINT-444743.
    STRINGi10090.ENSMUSP00000055823.

    Structurei

    3D structure databases

    ProteinModelPortaliP97814.
    SMRiP97814. Positions 7-285, 326-415.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 8278FCHPROSITE-ProRule annotationAdd
    BLAST
    Domaini358 – 41558SH3PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili94 – 13340Sequence AnalysisAdd
    BLAST
    Coiled coili162 – 21554Sequence AnalysisAdd
    BLAST

    Domaini

    The FCH domain is important for filament formation. The SH3 domain is not required for filament formation or localization to the uropod By similarity.By similarity

    Sequence similaritiesi

    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG303711.
    GeneTreeiENSGT00730000110901.
    HOGENOMiHOG000294218.
    HOVERGENiHBG052960.
    InParanoidiP97814.
    KOiK12804.
    OrthoDBiEOG7BCNBS.
    PhylomeDBiP97814.
    TreeFamiTF313677.

    Family and domain databases

    InterProiIPR001060. FCH_dom.
    IPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    [Graphical view]
    PfamiPF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMAQLQFRDA FWCRDFTAHT GYEVLLQRLL DGRKMCKDVE ELLRQRAQAE    50
    ERYGKELVQI ARKAGGQTEM NSLRTSFDSL KQQTENVGSA HIQLALALRE 100
    ELRSLEEFRE RQKEQRKKYE AIMDRVQKSK LSLYKKTMES KKAYDQKCRD 150
    ADDAEQAFER VSANGHQKQV EKSQNKAKQC KESATEAERV YRQNIEQLER 200
    ARTEWEQEHR TTCEAFQLQE FDRLTILRNA LWVHCNQLSM QCVKDDELYE 250
    EVRLTLEGCD VEGDINGFIQ SKSTGREPPA PVPYQNYYDR EVTPLIGSPS 300
    IQPSCGVIKR FSGLLHGSPK TTPSAPAAST ETLTPTPERN ELVYASIEVQ 350
    ATQGNLNSSA QDYRALYDYT AQNSDELDIS AGDILAVILE GEDGWWTVER 400
    NGQRGFVPGS YLEKL 415
    Length:415
    Mass (Da):47,590
    Last modified:May 1, 1997 - v1
    Checksum:i16C0329284D2739C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti301 – 3011I → V in AAH96761. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87814 mRNA. Translation: AAB48483.1.
    BC096761 mRNA. Translation: AAH96761.1.
    CCDSiCCDS40645.1.
    RefSeqiNP_035323.2. NM_011193.2.
    UniGeneiMm.2534.

    Genome annotation databases

    EnsembliENSMUST00000059206; ENSMUSP00000055823; ENSMUSG00000032322.
    GeneIDi19200.
    KEGGimmu:19200.
    UCSCiuc009psv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87814 mRNA. Translation: AAB48483.1 .
    BC096761 mRNA. Translation: AAH96761.1 .
    CCDSi CCDS40645.1.
    RefSeqi NP_035323.2. NM_011193.2.
    UniGenei Mm.2534.

    3D structure databases

    ProteinModelPortali P97814.
    SMRi P97814. Positions 7-285, 326-415.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202439. 2 interactions.
    IntActi P97814. 6 interactions.
    MINTi MINT-444743.
    STRINGi 10090.ENSMUSP00000055823.

    PTM databases

    PhosphoSitei P97814.

    Proteomic databases

    PaxDbi P97814.
    PRIDEi P97814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000059206 ; ENSMUSP00000055823 ; ENSMUSG00000032322 .
    GeneIDi 19200.
    KEGGi mmu:19200.
    UCSCi uc009psv.2. mouse.

    Organism-specific databases

    CTDi 9051.
    MGIi MGI:1321396. Pstpip1.

    Phylogenomic databases

    eggNOGi NOG303711.
    GeneTreei ENSGT00730000110901.
    HOGENOMi HOG000294218.
    HOVERGENi HBG052960.
    InParanoidi P97814.
    KOi K12804.
    OrthoDBi EOG7BCNBS.
    PhylomeDBi P97814.
    TreeFami TF313677.

    Miscellaneous databases

    NextBioi 295916.
    PROi P97814.
    SOURCEi Search...

    Gene expression databases

    Bgeei P97814.
    Genevestigatori P97814.

    Family and domain databases

    InterProi IPR001060. FCH_dom.
    IPR001452. SH3_domain.
    IPR013315. Spectrin_alpha_SH3.
    [Graphical view ]
    Pfami PF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    PR01887. SPECTRNALPHA.
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PSTPIP: a tyrosine phosphorylated cleavage furrow-associated protein that is a substrate for a PEST tyrosine phosphatase."
      Spencer S., Dowbenko D., Cheng J., Li W., Brush J., Utzig S., Simanis V., Lasky L.A.
      J. Cell Biol. 138:845-860(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PTPN18, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6NCr.
      Tissue: Hematopoietic stem cell.
    3. "Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated protein."
      Wu Y., Spencer S.D., Lasky L.A.
      J. Biol. Chem. 273:5765-5770(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH WAS, SUBCELLULAR LOCATION, PHOSPHORYLATION AT TYR-344, MUTAGENESIS OF TYR-344 AND TYR-367.
    4. "Cytoskeletal protein PSTPIP1 directs the PEST-type protein tyrosine phosphatase to the c-Abl kinase to mediate Abl dephosphorylation."
      Cong F., Spencer S., Cote J.F., Wu Y., Tremblay M.L., Lasky L.A., Goff S.P.
      Mol. Cell 6:1413-1423(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ABL1, PHOSPHORYLATION, MUTAGENESIS OF TYR-344 AND TYR-367.
    5. "PSTPIP is a substrate of PTP-PEST and serves as a scaffold guiding PTP-PEST toward a specific dephosphorylation of WASP."
      Cote J.-F., Chung P.L., Theberge J.-F., Halle M., Spencer S., Lasky L.A., Tremblay M.L.
      J. Biol. Chem. 277:2973-2986(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PTPN12, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION, MUTAGENESIS OF TYR-344 AND TYR-367.
    6. "The Wiskott-Aldrich syndrome protein acts downstream of CD2 and the CD2AP and PSTPIP1 adaptors to promote formation of the immunological synapse."
      Badour K., Zhang J., Shi F., McGavin M.K.H., Rampersad V., Hardy L.A., Field D., Siminovitch K.A.
      Immunity 18:141-154(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CD2; CD2AP AND WAS, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPPIP1_MOUSE
    AccessioniPrimary (citable) accession number: P97814
    Secondary accession number(s): Q4V9R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3