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P97813

- PLD2_MOUSE

UniProt

P97813 - PLD2_MOUSE

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Protein
Phospholipase D2
Gene
Pld2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulationi

Stimulated by phosphatidylinositol 4,5-bisphosphate. Is not responsive to either ADP-ribosylation factor-1 (ARF-1) or GTP-binding proteins such as RHOA.

GO - Molecular functioni

  1. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
  2. phosphatidylinositol binding Source: InterPro
  3. phospholipase D activity Source: MGI

GO - Biological processi

  1. G-protein coupled receptor internalization Source: MGI
  2. lipid catabolic process Source: UniProtKB-KW
  3. receptor-mediated endocytosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.4.4. 3474.
ReactomeiREACT_188640. Synthesis of PA.
REACT_199032. Synthesis of PG.
REACT_210240. Role of phospholipids in phagocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D2 (EC:3.1.4.4)
Short name:
PLD 2
Short name:
mPLD2
Alternative name(s):
Choline phosphatase 2
PLD1C
Phosphatidylcholine-hydrolyzing phospholipase D2
Gene namesi
Name:Pld2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:892877. Pld2.

Subcellular locationi

GO - Cellular componenti

  1. brush border membrane Source: MGI
  2. endoplasmic reticulum membrane Source: Reactome
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi11 – 111Y → F: 2-fold increase in basal phospholipase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Phospholipase D2
PRO_0000218806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated by FGR By similarity. Phosphorylated on Tyr-11; most likely by EGFR.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97813.
PRIDEiP97813.

PTM databases

PhosphoSiteiP97813.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in brain and lung.1 Publication

Developmental stagei

Expressed at high levels in the hippocampus at the earliest time at which it is defined as a structure and also in ventricular neural cells as well as differentiating neurons outside of the ventricular region. Expressed during development in lower levels in mesenchymal cells derived from the neural crest that are destined to form bones of the skull.

Gene expression databases

ArrayExpressiP97813.
BgeeiP97813.
CleanExiMM_PLD2.
GenevestigatoriP97813.

Interactioni

Subunit structurei

Interacts with PIP5K1B By similarity. Interacts with EGFR.

Structurei

3D structure databases

ProteinModelPortaliP97813.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 195131PX
Add
BLAST
Domaini203 – 311109PH
Add
BLAST
Domaini437 – 46428PLD phosphodiesterase 1
Add
BLAST
Domaini751 – 77828PLD phosphodiesterase 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni441 – 788348Catalytic
Add
BLAST

Sequence similaritiesi

Belongs to the phospholipase D family.
Contains 1 PH domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1502.
HOGENOMiHOG000246972.
HOVERGENiHBG006650.
KOiK01115.
OrthoDBiEOG7N63KT.
TreeFamiTF300589.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D_euk.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: At least 3 isoforms are produced.

Isoform 1 (identifier: P97813-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTVTQKNLFP YGDYLNSSQL HMEPDEVDTL REGEDPADRM HPYLAIYDLQ    50
PLKAHPLVFA PGVPVIAQVV GTERYTSGSK VGTCTLYSVR LTHGDFTWTT 100
KKKFRHFQEL HRDLQRHKVL MSLLPLARFA VTHSPAREAA AEDIPSLPRG 150
GSEGSARHTA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL 200
GSKGLEGVIR KRSGGHRVPG FTFCGRDQVC YRWSKRWLVV KDSFLLYMRP 250
ETGAISFVQL FDPGFEVQVG KRSTETRYGV RIDTSHRSLI LKCSSYRQAR 300
WWGQEITELA QGSGRDFLQL HQHDSYAPPR PGTLARWFVN GAGYFAAVAD 350
AILRAQEEIF ITDWWLSPEI YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL 400
LFKEVELALG INSGYSKRTL MLLHPNIKVM RHPDLVTLWA HHEKLLVVDQ 450
VVAFLGGLDL AFGRWDDVQY RLTDLGDPSE PVHLQTPTLG SDPAATPDLS 500
HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGV 550
AARDLARHFI QRWNFTKTTK ARYKTPLYPY LLPKSTSTAN NLPFMIPGGQ 600
CATVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG 650
RTVLNKVGDE IVDRILKAHE QGQCFRVYLL LPLLPGFEGD ISTGGGNSIQ 700
AILHFTYRTL CRGEHSILHR LKAAMGTAWR DYMSICGLRT HGELGGHPIS 750
ELIYIHSKML IADDRTVIIG SANINDRSLL GKRDSELAIL IKDTEMEPSL 800
MDGVEYQAGR FALSLRKHCF SVILGANTWP DLDLRDPVCD DFFQLWQETA 850
ENNATIYEQI FRCLPSNATR SLRALREYVA VESLATVSPS LAQSELAHIQ 900
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT 933
Length:933
Mass (Da):106,168
Last modified:July 1, 1997 - v2
Checksum:iBADE1E0DF2EAC9ED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87557 mRNA. Translation: AAC53173.1.
AF052294
, AF052291, AF052293, AF052292 Genomic DNA. Translation: AAC24519.1.
CCDSiCCDS24953.1. [P97813-1]
RefSeqiNP_032902.1. NM_008876.2. [P97813-1]
UniGeneiMm.260177.

Genome annotation databases

EnsembliENSMUST00000018429; ENSMUSP00000018429; ENSMUSG00000020828. [P97813-1]
GeneIDi18806.
KEGGimmu:18806.
UCSCiuc007jvg.1. mouse. [P97813-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87557 mRNA. Translation: AAC53173.1 .
AF052294
, AF052291 , AF052293 , AF052292 Genomic DNA. Translation: AAC24519.1 .
CCDSi CCDS24953.1. [P97813-1 ]
RefSeqi NP_032902.1. NM_008876.2. [P97813-1 ]
UniGenei Mm.260177.

3D structure databases

ProteinModelPortali P97813.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P97813.

Proteomic databases

PaxDbi P97813.
PRIDEi P97813.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000018429 ; ENSMUSP00000018429 ; ENSMUSG00000020828 . [P97813-1 ]
GeneIDi 18806.
KEGGi mmu:18806.
UCSCi uc007jvg.1. mouse. [P97813-1 ]

Organism-specific databases

CTDi 5338.
MGIi MGI:892877. Pld2.

Phylogenomic databases

eggNOGi COG1502.
HOGENOMi HOG000246972.
HOVERGENi HBG006650.
KOi K01115.
OrthoDBi EOG7N63KT.
TreeFami TF300589.

Enzyme and pathway databases

BRENDAi 3.1.4.4. 3474.
Reactomei REACT_188640. Synthesis of PA.
REACT_199032. Synthesis of PG.
REACT_210240. Role of phospholipids in phagocytosis.

Miscellaneous databases

ChiTaRSi PLD2. mouse.
NextBioi 295114.
PROi P97813.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97813.
Bgeei P97813.
CleanExi MM_PLD2.
Genevestigatori P97813.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D_euk.
IPR015679. PLipase_D_fam.
[Graphical view ]
PANTHERi PTHR18896. PTHR18896. 1 hit.
Pfami PF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view ]
PIRSFi PIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTi SM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view ]
SUPFAMi SSF64268. SSF64268. 1 hit.
PROSITEi PS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization."
    Colley W.C., Sung T.-C., Roll R., Jenco J.M., Hammond S.M., Altshuller Y.M., Bar-Sagi D., Morris A.J., Frohman M.A.
    Curr. Biol. 7:191-201(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Embryo and Neonatal brain.
  2. "Organization and alternative splicing of the murine phospholipase D2 gene."
    Redina O.E., Frohman M.A.
    Biochem. J. 331:845-851(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. "Cloning and expression analysis of murine phospholipase D1."
    Colley W.C., Altshuller Y.M., Sue-Ling C.K., Copeland N.G., Gilbert D.J., Jenkins N.A., Branch K.D., Tsirka S.E., Bollag R.J., Bollag W.B., Frohman M.A.
    Biochem. J. 326:745-753(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "PLD2 complexes with the EGF receptor and undergoes tyrosine phosphorylation at a single site upon agonist stimulation."
    Slaaby R., Jensen T., Hansen H.S., Frohman M.A., Seedorf K.
    J. Biol. Chem. 273:33722-33727(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-11, MUTAGENESIS OF TYR-11.

Entry informationi

Entry nameiPLD2_MOUSE
AccessioniPrimary (citable) accession number: P97813
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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