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Protein

Phospholipase D2

Gene

Pld2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulationi

Stimulated by phosphatidylinositol 4,5-bisphosphate. Is not responsive to either ADP-ribosylation factor-1 (ARF-1) or GTP-binding proteins such as RHOA.

GO - Molecular functioni

  1. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
  2. phosphatidylinositol binding Source: InterPro
  3. phospholipase D activity Source: MGI

GO - Biological processi

  1. G-protein coupled receptor internalization Source: MGI
  2. lipid catabolic process Source: UniProtKB-KW
  3. receptor-mediated endocytosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.4.4. 3474.
ReactomeiREACT_292208. Role of phospholipids in phagocytosis.
REACT_304133. Synthesis of PA.
REACT_352856. Synthesis of PG.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D2 (EC:3.1.4.4)
Short name:
PLD 2
Short name:
mPLD2
Alternative name(s):
Choline phosphatase 2
PLD1C
Phosphatidylcholine-hydrolyzing phospholipase D2
Gene namesi
Name:Pld2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:892877. Pld2.

Subcellular locationi

GO - Cellular componenti

  1. brush border membrane Source: MGI
  2. endoplasmic reticulum membrane Source: Reactome
  3. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi11 – 111Y → F: 2-fold increase in basal phospholipase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Phospholipase D2PRO_0000218806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated by FGR (By similarity). Phosphorylated on Tyr-11; most likely by EGFR.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97813.
PaxDbiP97813.
PRIDEiP97813.

PTM databases

PhosphoSiteiP97813.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in brain and lung.1 Publication

Developmental stagei

Expressed at high levels in the hippocampus at the earliest time at which it is defined as a structure and also in ventricular neural cells as well as differentiating neurons outside of the ventricular region. Expressed during development in lower levels in mesenchymal cells derived from the neural crest that are destined to form bones of the skull.

Gene expression databases

BgeeiP97813.
CleanExiMM_PLD2.
ExpressionAtlasiP97813. baseline and differential.
GenevestigatoriP97813.

Interactioni

Subunit structurei

Interacts with PIP5K1B (By similarity). Interacts with EGFR.By similarity

Structurei

3D structure databases

ProteinModelPortaliP97813.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 195131PXPROSITE-ProRule annotationAdd
BLAST
Domaini203 – 311109PHAdd
BLAST
Domaini437 – 46428PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
BLAST
Domaini751 – 77828PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni441 – 788348CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the phospholipase D family.Curated
Contains 1 PH domain.Curated
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1502.
HOGENOMiHOG000246972.
HOVERGENiHBG006650.
InParanoidiP97813.
KOiK01115.
OrthoDBiEOG7N63KT.
TreeFamiTF300589.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D1/D2.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: At least 3 isoforms are produced.

Isoform 1 (identifier: P97813-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVTQKNLFP YGDYLNSSQL HMEPDEVDTL REGEDPADRM HPYLAIYDLQ
60 70 80 90 100
PLKAHPLVFA PGVPVIAQVV GTERYTSGSK VGTCTLYSVR LTHGDFTWTT
110 120 130 140 150
KKKFRHFQEL HRDLQRHKVL MSLLPLARFA VTHSPAREAA AEDIPSLPRG
160 170 180 190 200
GSEGSARHTA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL
210 220 230 240 250
GSKGLEGVIR KRSGGHRVPG FTFCGRDQVC YRWSKRWLVV KDSFLLYMRP
260 270 280 290 300
ETGAISFVQL FDPGFEVQVG KRSTETRYGV RIDTSHRSLI LKCSSYRQAR
310 320 330 340 350
WWGQEITELA QGSGRDFLQL HQHDSYAPPR PGTLARWFVN GAGYFAAVAD
360 370 380 390 400
AILRAQEEIF ITDWWLSPEI YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL
410 420 430 440 450
LFKEVELALG INSGYSKRTL MLLHPNIKVM RHPDLVTLWA HHEKLLVVDQ
460 470 480 490 500
VVAFLGGLDL AFGRWDDVQY RLTDLGDPSE PVHLQTPTLG SDPAATPDLS
510 520 530 540 550
HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGV
560 570 580 590 600
AARDLARHFI QRWNFTKTTK ARYKTPLYPY LLPKSTSTAN NLPFMIPGGQ
610 620 630 640 650
CATVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG
660 670 680 690 700
RTVLNKVGDE IVDRILKAHE QGQCFRVYLL LPLLPGFEGD ISTGGGNSIQ
710 720 730 740 750
AILHFTYRTL CRGEHSILHR LKAAMGTAWR DYMSICGLRT HGELGGHPIS
760 770 780 790 800
ELIYIHSKML IADDRTVIIG SANINDRSLL GKRDSELAIL IKDTEMEPSL
810 820 830 840 850
MDGVEYQAGR FALSLRKHCF SVILGANTWP DLDLRDPVCD DFFQLWQETA
860 870 880 890 900
ENNATIYEQI FRCLPSNATR SLRALREYVA VESLATVSPS LAQSELAHIQ
910 920 930
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT
Length:933
Mass (Da):106,168
Last modified:June 30, 1997 - v2
Checksum:iBADE1E0DF2EAC9ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87557 mRNA. Translation: AAC53173.1.
AF052294
, AF052291, AF052293, AF052292 Genomic DNA. Translation: AAC24519.1.
CCDSiCCDS24953.1. [P97813-1]
RefSeqiNP_032902.1. NM_008876.3. [P97813-1]
UniGeneiMm.260177.

Genome annotation databases

EnsembliENSMUST00000018429; ENSMUSP00000018429; ENSMUSG00000020828. [P97813-1]
GeneIDi18806.
KEGGimmu:18806.
UCSCiuc007jvg.1. mouse. [P97813-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87557 mRNA. Translation: AAC53173.1.
AF052294
, AF052291, AF052293, AF052292 Genomic DNA. Translation: AAC24519.1.
CCDSiCCDS24953.1. [P97813-1]
RefSeqiNP_032902.1. NM_008876.3. [P97813-1]
UniGeneiMm.260177.

3D structure databases

ProteinModelPortaliP97813.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP97813.

Proteomic databases

MaxQBiP97813.
PaxDbiP97813.
PRIDEiP97813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018429; ENSMUSP00000018429; ENSMUSG00000020828. [P97813-1]
GeneIDi18806.
KEGGimmu:18806.
UCSCiuc007jvg.1. mouse. [P97813-1]

Organism-specific databases

CTDi5338.
MGIiMGI:892877. Pld2.

Phylogenomic databases

eggNOGiCOG1502.
HOGENOMiHOG000246972.
HOVERGENiHBG006650.
InParanoidiP97813.
KOiK01115.
OrthoDBiEOG7N63KT.
TreeFamiTF300589.

Enzyme and pathway databases

BRENDAi3.1.4.4. 3474.
ReactomeiREACT_292208. Role of phospholipids in phagocytosis.
REACT_304133. Synthesis of PA.
REACT_352856. Synthesis of PG.

Miscellaneous databases

ChiTaRSiPld2. mouse.
NextBioi295114.
PROiP97813.
SOURCEiSearch...

Gene expression databases

BgeeiP97813.
CleanExiMM_PLD2.
ExpressionAtlasiP97813. baseline and differential.
GenevestigatoriP97813.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D1/D2.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization."
    Colley W.C., Sung T.-C., Roll R., Jenco J.M., Hammond S.M., Altshuller Y.M., Bar-Sagi D., Morris A.J., Frohman M.A.
    Curr. Biol. 7:191-201(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Embryo and Neonatal brain.
  2. "Organization and alternative splicing of the murine phospholipase D2 gene."
    Redina O.E., Frohman M.A.
    Biochem. J. 331:845-851(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. "Cloning and expression analysis of murine phospholipase D1."
    Colley W.C., Altshuller Y.M., Sue-Ling C.K., Copeland N.G., Gilbert D.J., Jenkins N.A., Branch K.D., Tsirka S.E., Bollag R.J., Bollag W.B., Frohman M.A.
    Biochem. J. 326:745-753(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "PLD2 complexes with the EGF receptor and undergoes tyrosine phosphorylation at a single site upon agonist stimulation."
    Slaaby R., Jensen T., Hansen H.S., Frohman M.A., Seedorf K.
    J. Biol. Chem. 273:33722-33727(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-11, MUTAGENESIS OF TYR-11.

Entry informationi

Entry nameiPLD2_MOUSE
AccessioniPrimary (citable) accession number: P97813
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2001
Last sequence update: June 30, 1997
Last modified: March 31, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.