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P97813

- PLD2_MOUSE

UniProt

P97813 - PLD2_MOUSE

Protein

Phospholipase D2

Gene

Pld2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

    Catalytic activityi

    A phosphatidylcholine + H2O = choline + a phosphatidate.

    Enzyme regulationi

    Stimulated by phosphatidylinositol 4,5-bisphosphate. Is not responsive to either ADP-ribosylation factor-1 (ARF-1) or GTP-binding proteins such as RHOA.

    GO - Molecular functioni

    1. N-acylphosphatidylethanolamine-specific phospholipase D activity Source: UniProtKB-EC
    2. phosphatidylinositol binding Source: InterPro
    3. phospholipase D activity Source: MGI

    GO - Biological processi

    1. G-protein coupled receptor internalization Source: MGI
    2. lipid catabolic process Source: UniProtKB-KW
    3. receptor-mediated endocytosis Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi3.1.4.4. 3474.
    ReactomeiREACT_188640. Synthesis of PA.
    REACT_199032. Synthesis of PG.
    REACT_210240. Role of phospholipids in phagocytosis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase D2 (EC:3.1.4.4)
    Short name:
    PLD 2
    Short name:
    mPLD2
    Alternative name(s):
    Choline phosphatase 2
    PLD1C
    Phosphatidylcholine-hydrolyzing phospholipase D2
    Gene namesi
    Name:Pld2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:892877. Pld2.

    Subcellular locationi

    GO - Cellular componenti

    1. brush border membrane Source: MGI
    2. endoplasmic reticulum membrane Source: Reactome
    3. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi11 – 111Y → F: 2-fold increase in basal phospholipase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 933933Phospholipase D2PRO_0000218806Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111Phosphotyrosine1 Publication

    Post-translational modificationi

    Phosphorylated by FGR By similarity. Phosphorylated on Tyr-11; most likely by EGFR.By similarity1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP97813.
    PRIDEiP97813.

    PTM databases

    PhosphoSiteiP97813.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highest levels in brain and lung.1 Publication

    Developmental stagei

    Expressed at high levels in the hippocampus at the earliest time at which it is defined as a structure and also in ventricular neural cells as well as differentiating neurons outside of the ventricular region. Expressed during development in lower levels in mesenchymal cells derived from the neural crest that are destined to form bones of the skull.

    Gene expression databases

    ArrayExpressiP97813.
    BgeeiP97813.
    CleanExiMM_PLD2.
    GenevestigatoriP97813.

    Interactioni

    Subunit structurei

    Interacts with PIP5K1B By similarity. Interacts with EGFR.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP97813.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini65 – 195131PXPROSITE-ProRule annotationAdd
    BLAST
    Domaini203 – 311109PHAdd
    BLAST
    Domaini437 – 46428PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini751 – 77828PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni441 – 788348CatalyticAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phospholipase D family.Curated
    Contains 1 PH domain.Curated
    Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation
    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1502.
    HOGENOMiHOG000246972.
    HOVERGENiHBG006650.
    KOiK01115.
    OrthoDBiEOG7N63KT.
    TreeFamiTF300589.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.30.1520.10. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001683. Phox.
    IPR025202. PLD-like_dom.
    IPR001736. PLipase_D/transphosphatidylase.
    IPR016555. PLipase_D_euk.
    IPR015679. PLipase_D_fam.
    [Graphical view]
    PANTHERiPTHR18896. PTHR18896. 1 hit.
    PfamiPF00614. PLDc. 1 hit.
    PF13091. PLDc_2. 1 hit.
    PF00787. PX. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009376. Phospholipase_D_euk. 1 hit.
    SMARTiSM00233. PH. 1 hit.
    SM00155. PLDc. 2 hits.
    SM00312. PX. 1 hit.
    [Graphical view]
    SUPFAMiSSF64268. SSF64268. 1 hit.
    PROSITEiPS50035. PLD. 2 hits.
    PS50195. PX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: At least 3 isoforms are produced.

    Isoform 1 (identifier: P97813-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MTVTQKNLFP YGDYLNSSQL HMEPDEVDTL REGEDPADRM HPYLAIYDLQ    50
    PLKAHPLVFA PGVPVIAQVV GTERYTSGSK VGTCTLYSVR LTHGDFTWTT 100
    KKKFRHFQEL HRDLQRHKVL MSLLPLARFA VTHSPAREAA AEDIPSLPRG 150
    GSEGSARHTA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL 200
    GSKGLEGVIR KRSGGHRVPG FTFCGRDQVC YRWSKRWLVV KDSFLLYMRP 250
    ETGAISFVQL FDPGFEVQVG KRSTETRYGV RIDTSHRSLI LKCSSYRQAR 300
    WWGQEITELA QGSGRDFLQL HQHDSYAPPR PGTLARWFVN GAGYFAAVAD 350
    AILRAQEEIF ITDWWLSPEI YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL 400
    LFKEVELALG INSGYSKRTL MLLHPNIKVM RHPDLVTLWA HHEKLLVVDQ 450
    VVAFLGGLDL AFGRWDDVQY RLTDLGDPSE PVHLQTPTLG SDPAATPDLS 500
    HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGV 550
    AARDLARHFI QRWNFTKTTK ARYKTPLYPY LLPKSTSTAN NLPFMIPGGQ 600
    CATVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG 650
    RTVLNKVGDE IVDRILKAHE QGQCFRVYLL LPLLPGFEGD ISTGGGNSIQ 700
    AILHFTYRTL CRGEHSILHR LKAAMGTAWR DYMSICGLRT HGELGGHPIS 750
    ELIYIHSKML IADDRTVIIG SANINDRSLL GKRDSELAIL IKDTEMEPSL 800
    MDGVEYQAGR FALSLRKHCF SVILGANTWP DLDLRDPVCD DFFQLWQETA 850
    ENNATIYEQI FRCLPSNATR SLRALREYVA VESLATVSPS LAQSELAHIQ 900
    GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT 933
    Length:933
    Mass (Da):106,168
    Last modified:July 1, 1997 - v2
    Checksum:iBADE1E0DF2EAC9ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87557 mRNA. Translation: AAC53173.1.
    AF052294
    , AF052291, AF052293, AF052292 Genomic DNA. Translation: AAC24519.1.
    CCDSiCCDS24953.1. [P97813-1]
    RefSeqiNP_032902.1. NM_008876.2. [P97813-1]
    UniGeneiMm.260177.

    Genome annotation databases

    EnsembliENSMUST00000018429; ENSMUSP00000018429; ENSMUSG00000020828. [P97813-1]
    GeneIDi18806.
    KEGGimmu:18806.
    UCSCiuc007jvg.1. mouse. [P97813-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87557 mRNA. Translation: AAC53173.1 .
    AF052294
    , AF052291 , AF052293 , AF052292 Genomic DNA. Translation: AAC24519.1 .
    CCDSi CCDS24953.1. [P97813-1 ]
    RefSeqi NP_032902.1. NM_008876.2. [P97813-1 ]
    UniGenei Mm.260177.

    3D structure databases

    ProteinModelPortali P97813.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P97813.

    Proteomic databases

    PaxDbi P97813.
    PRIDEi P97813.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018429 ; ENSMUSP00000018429 ; ENSMUSG00000020828 . [P97813-1 ]
    GeneIDi 18806.
    KEGGi mmu:18806.
    UCSCi uc007jvg.1. mouse. [P97813-1 ]

    Organism-specific databases

    CTDi 5338.
    MGIi MGI:892877. Pld2.

    Phylogenomic databases

    eggNOGi COG1502.
    HOGENOMi HOG000246972.
    HOVERGENi HBG006650.
    KOi K01115.
    OrthoDBi EOG7N63KT.
    TreeFami TF300589.

    Enzyme and pathway databases

    BRENDAi 3.1.4.4. 3474.
    Reactomei REACT_188640. Synthesis of PA.
    REACT_199032. Synthesis of PG.
    REACT_210240. Role of phospholipids in phagocytosis.

    Miscellaneous databases

    ChiTaRSi PLD2. mouse.
    NextBioi 295114.
    PROi P97813.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97813.
    Bgeei P97813.
    CleanExi MM_PLD2.
    Genevestigatori P97813.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.30.1520.10. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001683. Phox.
    IPR025202. PLD-like_dom.
    IPR001736. PLipase_D/transphosphatidylase.
    IPR016555. PLipase_D_euk.
    IPR015679. PLipase_D_fam.
    [Graphical view ]
    PANTHERi PTHR18896. PTHR18896. 1 hit.
    Pfami PF00614. PLDc. 1 hit.
    PF13091. PLDc_2. 1 hit.
    PF00787. PX. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009376. Phospholipase_D_euk. 1 hit.
    SMARTi SM00233. PH. 1 hit.
    SM00155. PLDc. 2 hits.
    SM00312. PX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64268. SSF64268. 1 hit.
    PROSITEi PS50035. PLD. 2 hits.
    PS50195. PX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization."
      Colley W.C., Sung T.-C., Roll R., Jenco J.M., Hammond S.M., Altshuller Y.M., Bar-Sagi D., Morris A.J., Frohman M.A.
      Curr. Biol. 7:191-201(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Embryo and Neonatal brain.
    2. "Organization and alternative splicing of the murine phospholipase D2 gene."
      Redina O.E., Frohman M.A.
      Biochem. J. 331:845-851(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129.
    3. "Cloning and expression analysis of murine phospholipase D1."
      Colley W.C., Altshuller Y.M., Sue-Ling C.K., Copeland N.G., Gilbert D.J., Jenkins N.A., Branch K.D., Tsirka S.E., Bollag R.J., Bollag W.B., Frohman M.A.
      Biochem. J. 326:745-753(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "PLD2 complexes with the EGF receptor and undergoes tyrosine phosphorylation at a single site upon agonist stimulation."
      Slaaby R., Jensen T., Hansen H.S., Frohman M.A., Seedorf K.
      J. Biol. Chem. 273:33722-33727(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-11, MUTAGENESIS OF TYR-11.

    Entry informationi

    Entry nameiPLD2_MOUSE
    AccessioniPrimary (citable) accession number: P97813
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3