Reviewed, UniProtKB/Swiss-Prot P97813 (PLD2_MOUSE)

Names and origin

Protein names

Recommended name:
    Phospholipase D2
      Short name=PLD 2
      Short name=mPLD2
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 2
    Phosphatidylcholine-hydrolyzing phospholipase D2
    PLD1C

Gene names

Name:

Pld2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	933 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Enzyme regulation	Stimulated by phosphatidylinositol 4,5-bisphosphate. Is not responsive to either ADP-ribosylation factor-1 (ARF-1) or GTP-binding proteins such as RHOA.
Subunit structure	Interacts with PIP5K1A By similarity. Interacts with EGFR.
Subcellular location	Membrane; Peripheral membrane protein.
Tissue specificity	Ubiquitous. Highest levels in brain and lung. Ref.3
Developmental stage	Expressed at high levels in the hippocampus at the earliest time at which it is defined as a structure and also in ventricular neural cells as well as differentiating neurons outside of the ventricular region. Expressed during development in lower levels in mesenchymal cells derived from the neural crest that are destined to form bones of the skull.
Post-translational modification	Phosphorylated on Tyr-11; most likely by EGFR. Ref.4
Sequence similarities	Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor internalization Inferred from direct assay. Source: MGI cell communication Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	brush border membrane Inferred from direct assay. Source: MGI extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phosphoinositide binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: At least 3 isoforms are produced.

Isoform 1 (identifier: P97813-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 933	933	Phospholipase D2		PRO_0000218806
Regions
	Domain	65 – 195	131	PX
	Domain	203 – 311	109	PH
	Domain	437 – 464	28	PLD phosphodiesterase 1
	Domain	751 – 778	28	PLD phosphodiesterase 2
	Region	441 – 788	348	Catalytic
Amino acid modifications
	Modified residue	11	1	Phosphotyrosine Ref.4
Experimental info
	Mutagenesis	11	1	Y → F: 2-fold increase in basal phospholipase activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 1997. Version 2. Checksum: BADE1E0DF2EAC9ED	FASTA	933	106,168
	10 20 30 40 50 60 MTVTQKNLFP YGDYLNSSQL HMEPDEVDTL REGEDPADRM HPYLAIYDLQ PLKAHPLVFA 70 80 90 100 110 120 PGVPVIAQVV GTERYTSGSK VGTCTLYSVR LTHGDFTWTT KKKFRHFQEL HRDLQRHKVL 130 140 150 160 170 180 MSLLPLARFA VTHSPAREAA AEDIPSLPRG GSEGSARHTA SKQKYLENYL NRLLTMSFYR 190 200 210 220 230 240 NYHAMTEFLE VSQLSFIPDL GSKGLEGVIR KRSGGHRVPG FTFCGRDQVC YRWSKRWLVV 250 260 270 280 290 300 KDSFLLYMRP ETGAISFVQL FDPGFEVQVG KRSTETRYGV RIDTSHRSLI LKCSSYRQAR 310 320 330 340 350 360 WWGQEITELA QGSGRDFLQL HQHDSYAPPR PGTLARWFVN GAGYFAAVAD AILRAQEEIF 370 380 390 400 410 420 ITDWWLSPEI YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL LFKEVELALG INSGYSKRTL 430 440 450 460 470 480 MLLHPNIKVM RHPDLVTLWA HHEKLLVVDQ VVAFLGGLDL AFGRWDDVQY RLTDLGDPSE 490 500 510 520 530 540 PVHLQTPTLG SDPAATPDLS HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW 550 560 570 580 590 600 RDVGVVVHGV AARDLARHFI QRWNFTKTTK ARYKTPLYPY LLPKSTSTAN NLPFMIPGGQ 610 620 630 640 650 660 CATVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG RTVLNKVGDE 670 680 690 700 710 720 IVDRILKAHE QGQCFRVYLL LPLLPGFEGD ISTGGGNSIQ AILHFTYRTL CRGEHSILHR 730 740 750 760 770 780 LKAAMGTAWR DYMSICGLRT HGELGGHPIS ELIYIHSKML IADDRTVIIG SANINDRSLL 790 800 810 820 830 840 GKRDSELAIL IKDTEMEPSL MDGVEYQAGR FALSLRKHCF SVILGANTWP DLDLRDPVCD 850 860 870 880 890 900 DFFQLWQETA ENNATIYEQI FRCLPSNATR SLRALREYVA VESLATVSPS LAQSELAHIQ 910 920 930 GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT

References

[1]	"Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization." Colley W.C., Sung T.-C., Roll R., Jenco J.M., Hammond S.M., Altshuller Y.M., Bar-Sagi D., Morris A.J., Frohman M.A. Curr. Biol. 7:191-201(1997) [PubMed: 9395408] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Embryo and Neonatal brain.
[2]	"Organization and alternative splicing of the murine phospholipase D2 gene." Redina O.E., Frohman M.A. Biochem. J. 331:845-851(1998) [PubMed: 9560313] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[3]	"Cloning and expression analysis of murine phospholipase D1." Colley W.C., Altshuller Y.M., Sue-Ling C.K., Copeland N.G., Gilbert D.J., Jenkins N.A., Branch K.D., Tsirka S.E., Bollag R.J., Bollag W.B., Frohman M.A. Biochem. J. 326:745-753(1997) [PubMed: 9307024] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"PLD2 complexes with the EGF receptor and undergoes tyrosine phosphorylation at a single site upon agonist stimulation." Slaaby R., Jensen T., Hansen H.S., Frohman M.A., Seedorf K. J. Biol. Chem. 273:33722-33727(1998) [PubMed: 9837959] [Abstract] Cited for: PHOSPHORYLATION AT TYR-11, MUTAGENESIS OF TYR-11.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U87557 mRNA. Translation: AAC53173.1. AF052294 , AF052291, AF052293, AF052292 Genomic DNA. Translation: AAC24519.1.
IPI	IPI00469217.
RefSeq	NP_032902.1.
UniGene	Mm.260177
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P97813.
PTM databases
PhosphoSite	P97813.
Proteomic databases
PRIDE	P97813.
Genome annotation databases
Ensembl	ENSMUST00000018429; ENSMUSP00000018429; ENSMUSG00000020828; Mus musculus. [Genome view] ENSMUST00000108557; ENSMUSP00000104197; ENSMUSG00000020828; Mus musculus. [Genome view]
GeneID	18806.
KEGG	mmu:18806.
UCSC	uc007jvg.1. mouse.
Organism-specific databases
CTD	18806.
MGI	MGI:892877. Pld2.
Phylogenomic databases
HOVERGEN	P97813.
Enzyme and pathway databases
BRENDA	3.1.4.4. 244.
Gene expression databases
ArrayExpress	P97813.
Bgee	P97813.
CleanEx	MM_PLD2.
Genevestigator	P97813.
GermOnline	ENSMUSG00000020828. Mus musculus.
Family and domain databases
InterPro	IPR011993. PH_type. IPR015679. Phospholipase_D. IPR001849. Pleckstrin_homology. IPR001736. PLipase_D/transphosphatidylase. IPR016555. PLipase_D_euk. IPR001683. PX. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.1520.10. PX. 1 hit.
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00614. PLDc. 2 hits. PF00787. PX. 1 hit. [Graphical view]
PIRSF	PIRSF009376. Phospholipase_D_euk. 1 hit.
SMART	SM00233. PH. 1 hit. SM00155. PLDc. 2 hits. SM00312. PX. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. PS50035. PLD. 2 hits. PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295114.
SOURCE	Search...

Entry information

Entry name

PLD2_MOUSE

Accession

Primary (citable) accession number: P97813

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)