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Protein

Phospholipase D2

Gene

Pld2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulationi

Stimulated by phosphatidylinositol 4,5-bisphosphate. Is not responsive to either ADP-ribosylation factor-1 (ARF-1) or GTP-binding proteins such as RHOA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.4.4 3474
ReactomeiR-MMU-1483166 Synthesis of PA
R-MMU-2029485 Role of phospholipids in phagocytosis

Chemistry databases

SwissLipidsiSLP:000001045

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D2 (EC:3.1.4.4)
Short name:
PLD 2
Short name:
mPLD2
Alternative name(s):
Choline phosphatase 2
PLD1C
Phosphatidylcholine-hydrolyzing phospholipase D2
Gene namesi
Name:Pld2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:892877 Pld2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi11Y → F: 2-fold increase in basal phospholipase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002188061 – 933Phospholipase D2Add BLAST933

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylated by FGR (By similarity). Phosphorylated on Tyr-11; most likely by EGFR.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP97813
PeptideAtlasiP97813
PRIDEiP97813

PTM databases

iPTMnetiP97813
PhosphoSitePlusiP97813
SwissPalmiP97813

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in brain and lung.1 Publication

Developmental stagei

Expressed at high levels in the hippocampus at the earliest time at which it is defined as a structure and also in ventricular neural cells as well as differentiating neurons outside of the ventricular region. Expressed during development in lower levels in mesenchymal cells derived from the neural crest that are destined to form bones of the skull.

Gene expression databases

BgeeiENSMUSG00000020828
CleanExiMM_PLD2
ExpressionAtlasiP97813 baseline and differential
GenevisibleiP97813 MM

Interactioni

Subunit structurei

Interacts with PIP5K1B (By similarity). Interacts with EGFR.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000018429

Structurei

3D structure databases

ProteinModelPortaliP97813
SMRiP97813
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 195PXPROSITE-ProRule annotationAdd BLAST131
Domaini203 – 311PHAdd BLAST109
Domaini437 – 464PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST28
Domaini751 – 778PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni441 – 788CatalyticAdd BLAST348

Sequence similaritiesi

Belongs to the phospholipase D family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329 Eukaryota
COG1502 LUCA
GeneTreeiENSGT00390000008356
HOGENOMiHOG000246972
HOVERGENiHBG006650
InParanoidiP97813
KOiK01115
TreeFamiTF300589

Family and domain databases

Gene3Di2.30.29.30, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001683 Phox
IPR025202 PLD-like_dom
IPR001736 PLipase_D/transphosphatidylase
IPR016555 PLipase_D_euk
IPR015679 PLipase_D_fam
IPR036871 PX_dom_sf
PANTHERiPTHR18896 PTHR18896, 2 hits
PfamiView protein in Pfam
PF00614 PLDc, 1 hit
PF13091 PLDc_2, 1 hit
PF00787 PX, 1 hit
PIRSFiPIRSF009376 Phospholipase_D_euk, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00155 PLDc, 2 hits
SM00312 PX, 1 hit
SUPFAMiSSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50035 PLD, 2 hits
PS50195 PX, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: At least 3 isoforms are produced.
Isoform 1 (identifier: P97813-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVTQKNLFP YGDYLNSSQL HMEPDEVDTL REGEDPADRM HPYLAIYDLQ
60 70 80 90 100
PLKAHPLVFA PGVPVIAQVV GTERYTSGSK VGTCTLYSVR LTHGDFTWTT
110 120 130 140 150
KKKFRHFQEL HRDLQRHKVL MSLLPLARFA VTHSPAREAA AEDIPSLPRG
160 170 180 190 200
GSEGSARHTA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL
210 220 230 240 250
GSKGLEGVIR KRSGGHRVPG FTFCGRDQVC YRWSKRWLVV KDSFLLYMRP
260 270 280 290 300
ETGAISFVQL FDPGFEVQVG KRSTETRYGV RIDTSHRSLI LKCSSYRQAR
310 320 330 340 350
WWGQEITELA QGSGRDFLQL HQHDSYAPPR PGTLARWFVN GAGYFAAVAD
360 370 380 390 400
AILRAQEEIF ITDWWLSPEI YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL
410 420 430 440 450
LFKEVELALG INSGYSKRTL MLLHPNIKVM RHPDLVTLWA HHEKLLVVDQ
460 470 480 490 500
VVAFLGGLDL AFGRWDDVQY RLTDLGDPSE PVHLQTPTLG SDPAATPDLS
510 520 530 540 550
HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGV
560 570 580 590 600
AARDLARHFI QRWNFTKTTK ARYKTPLYPY LLPKSTSTAN NLPFMIPGGQ
610 620 630 640 650
CATVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG
660 670 680 690 700
RTVLNKVGDE IVDRILKAHE QGQCFRVYLL LPLLPGFEGD ISTGGGNSIQ
710 720 730 740 750
AILHFTYRTL CRGEHSILHR LKAAMGTAWR DYMSICGLRT HGELGGHPIS
760 770 780 790 800
ELIYIHSKML IADDRTVIIG SANINDRSLL GKRDSELAIL IKDTEMEPSL
810 820 830 840 850
MDGVEYQAGR FALSLRKHCF SVILGANTWP DLDLRDPVCD DFFQLWQETA
860 870 880 890 900
ENNATIYEQI FRCLPSNATR SLRALREYVA VESLATVSPS LAQSELAHIQ
910 920 930
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT
Length:933
Mass (Da):106,168
Last modified:July 1, 1997 - v2
Checksum:iBADE1E0DF2EAC9ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87557 mRNA Translation: AAC53173.1
AF052294
, AF052291, AF052293, AF052292 Genomic DNA Translation: AAC24519.1
CCDSiCCDS24953.1 [P97813-1]
RefSeqiNP_032902.1, NM_008876.3 [P97813-1]
XP_011247096.1, XM_011248794.2 [P97813-1]
UniGeneiMm.260177

Genome annotation databases

EnsembliENSMUST00000018429; ENSMUSP00000018429; ENSMUSG00000020828 [P97813-1]
GeneIDi18806
KEGGimmu:18806
UCSCiuc007jvg.2 mouse [P97813-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPLD2_MOUSE
AccessioniPrimary (citable) accession number: P97813
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 1, 1997
Last modified: June 20, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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