Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P97813 (PLD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholipase D2

Short name=PLD 2
Short name=mPLD2
EC=3.1.4.4
Alternative name(s):
Choline phosphatase 2
PLD1C
Phosphatidylcholine-hydrolyzing phospholipase D2
Gene names
Name:Pld2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length933 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

Catalytic activity

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulation

Stimulated by phosphatidylinositol 4,5-bisphosphate. Is not responsive to either ADP-ribosylation factor-1 (ARF-1) or GTP-binding proteins such as RHOA.

Subunit structure

Interacts with PIP5K1B By similarity. Interacts with EGFR.

Subcellular location

Membrane; Peripheral membrane protein.

Tissue specificity

Ubiquitous. Highest levels in brain and lung. Ref.3

Developmental stage

Expressed at high levels in the hippocampus at the earliest time at which it is defined as a structure and also in ventricular neural cells as well as differentiating neurons outside of the ventricular region. Expressed during development in lower levels in mesenchymal cells derived from the neural crest that are destined to form bones of the skull.

Post-translational modification

Phosphorylated by FGR By similarity. Phosphorylated on Tyr-11; most likely by EGFR. Ref.4

Sequence similarities

Belongs to the phospholipase D family.

Contains 1 PH domain.

Contains 2 PLD phosphodiesterase domains.

Contains 1 PX (phox homology) domain.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: At least 3 isoforms are produced.
Isoform 1 (identifier: P97813-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 933933Phospholipase D2
PRO_0000218806

Regions

Domain65 – 195131PX
Domain203 – 311109PH
Domain437 – 46428PLD phosphodiesterase 1
Domain751 – 77828PLD phosphodiesterase 2
Region441 – 788348Catalytic

Amino acid modifications

Modified residue111Phosphotyrosine Ref.4

Experimental info

Mutagenesis111Y → F: 2-fold increase in basal phospholipase activity. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1997. Version 2.
Checksum: BADE1E0DF2EAC9ED

FASTA933106,168
        10         20         30         40         50         60 
MTVTQKNLFP YGDYLNSSQL HMEPDEVDTL REGEDPADRM HPYLAIYDLQ PLKAHPLVFA 

        70         80         90        100        110        120 
PGVPVIAQVV GTERYTSGSK VGTCTLYSVR LTHGDFTWTT KKKFRHFQEL HRDLQRHKVL 

       130        140        150        160        170        180 
MSLLPLARFA VTHSPAREAA AEDIPSLPRG GSEGSARHTA SKQKYLENYL NRLLTMSFYR 

       190        200        210        220        230        240 
NYHAMTEFLE VSQLSFIPDL GSKGLEGVIR KRSGGHRVPG FTFCGRDQVC YRWSKRWLVV 

       250        260        270        280        290        300 
KDSFLLYMRP ETGAISFVQL FDPGFEVQVG KRSTETRYGV RIDTSHRSLI LKCSSYRQAR 

       310        320        330        340        350        360 
WWGQEITELA QGSGRDFLQL HQHDSYAPPR PGTLARWFVN GAGYFAAVAD AILRAQEEIF 

       370        380        390        400        410        420 
ITDWWLSPEI YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL LFKEVELALG INSGYSKRTL 

       430        440        450        460        470        480 
MLLHPNIKVM RHPDLVTLWA HHEKLLVVDQ VVAFLGGLDL AFGRWDDVQY RLTDLGDPSE 

       490        500        510        520        530        540 
PVHLQTPTLG SDPAATPDLS HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW 

       550        560        570        580        590        600 
RDVGVVVHGV AARDLARHFI QRWNFTKTTK ARYKTPLYPY LLPKSTSTAN NLPFMIPGGQ 

       610        620        630        640        650        660 
CATVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG RTVLNKVGDE 

       670        680        690        700        710        720 
IVDRILKAHE QGQCFRVYLL LPLLPGFEGD ISTGGGNSIQ AILHFTYRTL CRGEHSILHR 

       730        740        750        760        770        780 
LKAAMGTAWR DYMSICGLRT HGELGGHPIS ELIYIHSKML IADDRTVIIG SANINDRSLL 

       790        800        810        820        830        840 
GKRDSELAIL IKDTEMEPSL MDGVEYQAGR FALSLRKHCF SVILGANTWP DLDLRDPVCD 

       850        860        870        880        890        900 
DFFQLWQETA ENNATIYEQI FRCLPSNATR SLRALREYVA VESLATVSPS LAQSELAHIQ 

       910        920        930 
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT 

« Hide

References

[1]"Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization."
Colley W.C., Sung T.-C., Roll R., Jenco J.M., Hammond S.M., Altshuller Y.M., Bar-Sagi D., Morris A.J., Frohman M.A.
Curr. Biol. 7:191-201(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Embryo and Neonatal brain.
[2]"Organization and alternative splicing of the murine phospholipase D2 gene."
Redina O.E., Frohman M.A.
Biochem. J. 331:845-851(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[3]"Cloning and expression analysis of murine phospholipase D1."
Colley W.C., Altshuller Y.M., Sue-Ling C.K., Copeland N.G., Gilbert D.J., Jenkins N.A., Branch K.D., Tsirka S.E., Bollag R.J., Bollag W.B., Frohman M.A.
Biochem. J. 326:745-753(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"PLD2 complexes with the EGF receptor and undergoes tyrosine phosphorylation at a single site upon agonist stimulation."
Slaaby R., Jensen T., Hansen H.S., Frohman M.A., Seedorf K.
J. Biol. Chem. 273:33722-33727(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-11, MUTAGENESIS OF TYR-11.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U87557 mRNA. Translation: AAC53173.1.
AF052294 expand/collapse EMBL AC list , AF052291, AF052293, AF052292 Genomic DNA. Translation: AAC24519.1.
CCDSCCDS24953.1. [P97813-1]
RefSeqNP_032902.1. NM_008876.2. [P97813-1]
UniGeneMm.260177.

3D structure databases

ProteinModelPortalP97813.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP97813.

Proteomic databases

PaxDbP97813.
PRIDEP97813.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000018429; ENSMUSP00000018429; ENSMUSG00000020828. [P97813-1]
GeneID18806.
KEGGmmu:18806.
UCSCuc007jvg.1. mouse. [P97813-1]

Organism-specific databases

CTD5338.
MGIMGI:892877. Pld2.

Phylogenomic databases

eggNOGCOG1502.
HOGENOMHOG000246972.
HOVERGENHBG006650.
KOK01115.
OrthoDBEOG7N63KT.
TreeFamTF300589.

Enzyme and pathway databases

BRENDA3.1.4.4. 3474.
ReactomeREACT_188937. Metabolism.

Gene expression databases

ArrayExpressP97813.
BgeeP97813.
CleanExMM_PLD2.
GenevestigatorP97813.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
3.30.1520.10. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001683. Phox.
IPR025202. PLD-like_dom.
IPR001849. Pleckstrin_homology.
IPR001736. PLipase_D/transphosphatidylase.
IPR016555. PLipase_D_euk.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERPTHR18896. PTHR18896. 1 hit.
PfamPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
PIRSFPIRSF009376. Phospholipase_D_euk. 1 hit.
SMARTSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF64268. SSF64268. 1 hit.
PROSITEPS50035. PLD. 2 hits.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPLD2. mouse.
NextBio295114.
PROP97813.
SOURCESearch...

Entry information

Entry namePLD2_MOUSE
AccessionPrimary (citable) accession number: P97813
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot