P97812 (IHH_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 127.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Indian hedgehog protein Short name=IHH Alternative name(s): HHG-2 Cleaved into the following 2 chains: | ||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 411 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). |
| Subcellular location | Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side By similarity. Note: The N-terminal peptide remains associated with the cell surface By similarity. Indian hedgehog protein C-product: Secreted › extracellular space By similarity. Note: The C-terminal peptide diffuses from the cell By similarity. |
| Tissue specificity | In the embryo, detected in the developing gut, the growth zone of cartilage of developing long bones, epithelium and urogenital sinus. In the adult kidney, found in proximal convoluted and proximal straight tubule. |
| Developmental stage | Detected at 10 dpc in developing gut, at 14.5 days dpc in the cartilage primordium and in the developing urogenital sinus. Expression increases with gestional age in kidney and duodenum, becoming maximal in adulthood. |
| Post-translational modification | The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity By similarity. Cholesterylation is required for N-product targeting to lipid rafts and multimerization By similarity. N-palmitoylation is required for N-product multimerization and full activity By similarity. |
| Sequence similarities | Belongs to the hedgehog family. |
| Sequence caution | The sequence AAB49692.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 27 | 27 | Potential | ||||||
| Chain | 28 – 411 | 384 | Indian hedgehog protein | PRO_0000013232 | |||||
| Chain | 28 – 202 | 175 | Indian hedgehog protein N-product | PRO_0000013233 | |||||
| Chain | 203 – 411 | 209 | Indian hedgehog protein C-product | PRO_0000013234 | |||||
Sites | |||||||||
| Site | 202 – 203 | 2 | Cleavage; by autolysis | ||||||
| Site | 248 | 1 | Involved in cholesterol transfer By similarity | ||||||
| Site | 272 | 1 | Involved in auto-cleavage By similarity | ||||||
| Site | 275 | 1 | Essential for auto-cleavage By similarity | ||||||
Amino acid modifications | |||||||||
| Lipidation | 28 | 1 | N-palmitoyl cysteine By similarity | ||||||
| Lipidation | 202 | 1 | Cholesterol glycine ester By similarity | ||||||
| Glycosylation | 282 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 383 | 1 | W → S Ref.2 | ||||||
Sequences
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References
| [1] | "Post-translational processing and renal expression of mouse indian hedgehog." Valentini R.P., Brookhiser W.T., Park J., Yang T., Briggs J., Dressler G., Holzman L.B. J. Biol. Chem. 272:8466-8473(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], AUTOCATALYTIC CLEAVAGE. Strain: CD-1. Tissue: Kidney. |
| [2] | "Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity." Echelard Y., Epstein D.J., St Jacques B., Shen L., Mohler J., McMahon J.A., McMahon A.P. Cell 75:1417-1430(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 76-411. Strain: C57BL/6J. Tissue: Embryo. |
| [3] | St Jacques B. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. |
| [4] | "Products, genetic linkage and limb patterning activity of a murine hedgehog gene." Chang D.T., Lopez A., von Kessler D.P., Chiang C., Simandl B.K., Zhao R., Seldin M.F., Fallon J.F., Beachy P.A. Development 120:3339-3353(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 124-172. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U85610 mRNA. Translation: AAB49692.1. Different initiation. X76291 mRNA. Translation: CAA53923.1. |
| IPI | IPI00987856. |
| PIR | C49425. |
| RefSeq | NP_034674.1. NM_010544.2. |
| UniGene | Mm.439736. |
3D structure databases | |
| ProteinModelPortal | P97812. |
| SMR | P97812. Positions 41-197, 203-349. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-59800N. |
Protein family/group databases | |
| MEROPS | C46.003. |
Proteomic databases | |
| PaxDb | P97812. |
| PRIDE | P97812. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 16147. |
| KEGG | mmu:16147. |
Organism-specific databases | |
| CTD | 3549. |
| MGI | MGI:96533. Ihh. |
Phylogenomic databases | |
| eggNOG | NOG250647. |
| HOVERGEN | HBG005480. |
| InParanoid | P97812. |
| KO | K11989. |
| OrthoDB | EOG46WZ8G. |
Gene expression databases | |
| ArrayExpress | P97812. |
| Bgee | P97812. |
| Genevestigator | P97812. |
| GermOnline | ENSMUSG00000006538. Mus musculus. |
Family and domain databases | |
| Gene3D | 3.30.1380.10. 1 hit. |
| InterPro | IPR001657. Hedgehog. IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom. IPR000320. Hedgehog_signaling_dom. IPR001767. Hint_dom. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006141. Intein_splice_site. [Graphical view] |
| Pfam | PF01085. HH_signal. 1 hit. PF01079. Hint. 1 hit. [Graphical view] |
| PIRSF | PIRSF009400. Peptidase_C46. 1 hit. |
| PRINTS | PR00632. SONICHHOG. |
| SMART | SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view] |
| SUPFAM | SSF55166. Hedgehog_sig_N. 1 hit. |
| PROSITE | PS50817. INTEIN_N_TER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 288931. |
| SOURCE | Search... |
Entry information
| Entry name | IHH_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P97812 Secondary accession number(s): Q61724 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |