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P97794 (KCNJ8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 8
Alternative name(s):
Inward rectifier K(+) channel Kir6.1
Potassium channel, inwardly rectifying subfamily J member 8
uKATP-1
Gene names
Name:Kcnj8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ8 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 424424ATP-sensitive inward rectifier potassium channel 8
PRO_0000154948

Regions

Topological domain1 – 6969Cytoplasmic By similarity
Transmembrane70 – 9425Helical; Name=M1; By similarity
Topological domain95 – 12632Extracellular By similarity
Intramembrane127 – 13812Helical; Pore-forming; Name=H5; By similarity
Intramembrane139 – 1457Pore-forming; By similarity
Topological domain146 – 1549Extracellular By similarity
Transmembrane155 – 17622Helical; Name=M2; By similarity
Topological domain177 – 424248Cytoplasmic By similarity
Motif140 – 1456Selectivity filter By similarity

Sites

Site1701Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P97794 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: BF02D1CC31970379

FASTA42447,980
        10         20         30         40         50         60 
MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI REQGRFLQDI 

        70         80         90        100        110        120 
FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD IYAYMEKGTM EKSGLESAVC 

       130        140        150        160        170        180 
VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE ECPLAITVLI LQNIVGLIIN AVMLGCIFMK 

       190        200        210        220        230        240 
TAQAHRRAET LIFSRHAVIA VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV 

       250        260        270        280        290        300 
VPIHQQDIPV DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLANQDL EVIVILEGVV 

       310        320        330        340        350        360 
ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVRVAA PRCSARELDE 

       370        380        390        400        410        420 
KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRNNSIRR NNSSLMVPKV QFMTPEGNQC 


PSES 

« Hide

References

« Hide 'large scale' references
[1]"Sulphonylurea receptor 2B and Kir6.1 form a sulphonylurea-sensitive but ATP-insensitive K+ channel."
Yamada M., Isomoto S., Matsumoto S., Kondo C., Shindo T., Horio Y., Kurachi Y.
J. Physiol. (Lond.) 499:715-720(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D88159 mRNA. Translation: BAA13550.1.
BC003756 mRNA. Translation: AAH03756.1.
CCDSCCDS20685.1.
RefSeqNP_032454.1. NM_008428.4.
XP_006506978.1. XM_006506915.1.
UniGeneMm.1482.

3D structure databases

ProteinModelPortalP97794.
SMRP97794. Positions 35-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000032374.

Chemistry

GuidetoPHARMACOLOGY441.

PTM databases

PhosphoSiteP97794.

Proteomic databases

PRIDEP97794.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032374; ENSMUSP00000032374; ENSMUSG00000030247.
GeneID16523.
KEGGmmu:16523.
UCSCuc009epk.1. mouse.

Organism-specific databases

CTD3764.
MGIMGI:1100508. Kcnj8.

Phylogenomic databases

eggNOGNOG72812.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP97794.
KOK05001.
OMAVIAVRNN.
OrthoDBEOG7XPZ5K.
PhylomeDBP97794.
TreeFamTF313676.

Gene expression databases

BgeeP97794.
GenevestigatorP97794.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003278. K_chnl_inward-rec_Kir6.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01331. KIR61CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

ChiTaRSKCNJ8. mouse.
NextBio289911.
PROP97794.
SOURCESearch...

Entry information

Entry nameKCNJ8_MOUSE
AccessionPrimary (citable) accession number: P97794
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot