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Protein

Metabotropic glutamate receptor 1

Gene

Grm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum (By. similarity).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741GlutamateBy similarity
Binding sitei165 – 1651GlutamateBy similarity
Binding sitei236 – 2361GlutamateBy similarity
Binding sitei318 – 3181GlutamateBy similarity
Binding sitei409 – 4091GlutamateBy similarity

GO - Molecular functioni

  1. glutamate receptor activity Source: UniProtKB
  2. G-protein coupled receptor activity Source: UniProtKB-KW
  3. PLC activating G-protein coupled glutamate receptor activity Source: MGI

GO - Biological processi

  1. activation of MAPK activity Source: UniProtKB
  2. activation of MAPKK activity Source: UniProtKB
  3. cellular response to electrical stimulus Source: MGI
  4. dimeric G-protein coupled receptor signaling pathway Source: UniProtKB
  5. G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  6. G-protein coupled receptor signaling pathway Source: UniProtKB
  7. locomotory behavior Source: UniProtKB
  8. phospholipase C-activating G-protein coupled glutamate receptor signaling pathway Source: GOC
  9. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: UniProtKB
  10. protein kinase C-activating G-protein coupled receptor signaling pathway Source: GO_Central
  11. regulation of MAPK cascade Source: UniProtKB
  12. regulation of sensory perception of pain Source: UniProtKB
  13. regulation of synaptic transmission, glutamatergic Source: GO_Central
  14. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_336829. Class C/3 (Metabotropic glutamate/pheromone receptors).
REACT_347934. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 1
Short name:
mGluR1
Gene namesi
Name:Grm1
Synonyms:Gprc1a, Mglur1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1351338. Grm1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 592572ExtracellularBy similarityAdd
BLAST
Transmembranei593 – 61523Helical; Name=1By similarityAdd
BLAST
Topological domaini616 – 62914CytoplasmicBy similarityAdd
BLAST
Transmembranei630 – 65021Helical; Name=2By similarityAdd
BLAST
Topological domaini651 – 6588ExtracellularBy similarity
Transmembranei659 – 68022Helical; Name=3By similarityAdd
BLAST
Topological domaini681 – 70323CytoplasmicBy similarityAdd
BLAST
Transmembranei704 – 72724Helical; Name=4By similarityAdd
BLAST
Topological domaini728 – 75023ExtracellularBy similarityAdd
BLAST
Transmembranei751 – 77222Helical; Name=5By similarityAdd
BLAST
Topological domaini773 – 78513CytoplasmicBy similarityAdd
BLAST
Transmembranei786 – 80722Helical; Name=6By similarityAdd
BLAST
Topological domaini808 – 8158ExtracellularBy similarity
Transmembranei816 – 84025Helical; Name=7By similarityAdd
BLAST
Topological domaini841 – 1199359CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. dendrite Source: UniProtKB
  2. G-protein coupled receptor dimeric complex Source: MGI
  3. G-protein coupled receptor homodimeric complex Source: MGI
  4. integral component of plasma membrane Source: UniProtKB
  5. neuronal cell body Source: UniProtKB
  6. neuron projection Source: BHF-UCL
  7. nucleus Source: MGI
  8. postsynaptic density Source: MGI
  9. postsynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 11991179Metabotropic glutamate receptor 1PRO_0000012923Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 109By similarity
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi140 – 140InterchainBy similarity
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi289 ↔ 291By similarity
Disulfide bondi378 ↔ 394By similarity
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi432 ↔ 439By similarity
Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi657 ↔ 746By similarity
Glycosylationi747 – 7471N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP97772.
PRIDEiP97772.

PTM databases

PhosphoSiteiP97772.

Expressioni

Gene expression databases

BgeeiP97772.
CleanExiMM_GRM1.
ExpressionAtlasiP97772. baseline and differential.
GenevestigatoriP97772.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP (By similarity).By similarity

Protein-protein interaction databases

BioGridi200073. 8 interactions.
IntActiP97772. 4 interactions.
MINTiMINT-4595385.

Structurei

3D structure databases

ProteinModelPortaliP97772.
SMRiP97772. Positions 35-578, 581-846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1883Glutamate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1014 – 104128Gln/Pro-richAdd
BLAST
Compositional biasi1073 – 108715Gln/Pro-richAdd
BLAST
Compositional biasi1101 – 113535Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi1147 – 119953Ser-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295200.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218636.
HOVERGENiHBG107965.
InParanoidiP97772.
KOiK04603.
OMAiKTNETAC.
OrthoDBiEOG7Z0JXG.
PhylomeDBiP97772.
TreeFamiTF313240.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001256. GPCR_3_mtglu_rcpt_1.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01051. MTABOTROPC1R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97772-1) [UniParc]FASTAAdd to basket

Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRLLLIFFP MIFLEMSILP RMPDRKVLLA GASSQRSVAR MDGDVIIGAL
60 70 80 90 100
FSVHHQPPAE KVPERKCGEI REQYGIQRVE AMFHTLDKIN ADPVLLPNIT
110 120 130 140 150
LGSEIRDSCW HSSVALEQSI EFIRDSLISI RDEKDGLNRC LPDGQTLPPG
160 170 180 190 200
RTKKPIAGVI GPGSSSVAIQ VQNLLQLFDI PQIAYSATSI DLSDKTLYKY
210 220 230 240 250
FLRVVPSDTL QARAMLDIVK RYNWTYVSAV HTEGNYGESG MDAFKELAAQ
260 270 280 290 300
EGLCIAHSDK IYSNAGEKSF DRLLRKLRER LPKARVVVCF CEGMTVRGLL
310 320 330 340 350
SAMRRLGVVG EFSLIGSDGW ADRDEVIEGY EVEANGGITI KLQSPEVRSF
360 370 380 390 400
DDYFLKLRLD TNTRNPWFPE FWQHRFQCRL PGHLLENPNF KKVCTGNESL
410 420 430 440 450
EENYVQDSKM GFVINAIYAM AHGLQNMHHA LCPGYVGLCD AMKPIDGRKL
460 470 480 490 500
LDFLIKSSFV GVSGEEVWFD EKGDAPGRYD IMNLQYTEAN RYDYVHVGTW
510 520 530 540 550
HEGVLNIDDY KIQMNKSGMV RSVCSEPCLK GQIKVIRKGE VSCCWICTAC
560 570 580 590 600
KENEFVQDEF TCRACDLGWW PNAELTGCEP ITIRYLEWSD IESIIAIAFS
610 620 630 640 650
CLGILVTLFV TLIFVLYRDT PVVKSSSREL CYIILAGIFL GYVCPFTLIA
660 670 680 690 700
KPTTTSCYLQ RLLVGLSSAM CYSALVTKTN RIARILAGSK KKICTRKPRF
710 720 730 740 750
MSAWAQVIIA SILISVQLTL VVTLIIMEPP MPILSYPSIK EVYLICNTSN
760 770 780 790 800
LGVVAPVGYN GLLIMSCTYY AFKTRNVPAN FNEAKYIAFT MYTTCIIWLA
810 820 830 840 850
FVPIYFGSNY KIITTCFAVS LSVTVALGCM FTPKMYIIIA KPERNVRSAF
860 870 880 890 900
TTSDVVRMHV GDGKLPCRSN TFLNIFRRKK PGAGNANSNG KSVSWSEPGG
910 920 930 940 950
RQAPKGQHVW QRLSVHVKTN ETACNQTAVI KPLTKSYQGS GKSLTFSDAS
960 970 980 990 1000
TKTLYNVEEE DNTPSTHFSP PSSPSMVVHR RGPPVATTPP LPPHLSAEET
1010 1020 1030 1040 1050
PLFLADSVIP KGLPPPLPQQ QQQPPPQPPP QQPKSLMDQL QGVVTNFGSG
1060 1070 1080 1090 1100
IPDFHAVLAG PGTPGNGLRS LYPPPPPPQH LQMLPLQLST FREEPISPPG
1110 1120 1130 1140 1150
EDDDDDSSER FKLLQEFVYE REGNTEEDDL EEEEDLPAAS KLTPEDSPAL
1160 1170 1180 1190
TPPSPFRDSV ASGSSVPSSP VSESVLCTPP NVTYASVILR DYKQSSSTL
Length:1,199
Mass (Da):133,212
Last modified:April 11, 2003 - v2
Checksum:iFE5370AF160CC16E
GO
Isoform 2 (identifier: P97772-2) [UniParc]FASTAAdd to basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     887-906: NSNGKSVSWSEPGGRQAPKG → KKRQPEFSPSSQCPSAHVQL
     907-1199: Missing.

Show »
Length:906
Mass (Da):101,710
Checksum:iB9223F00A9C6851C
GO
Isoform 3 (identifier: P97772-3) [UniParc]FASTAAdd to basket

Also known as: E55

The sequence of this isoform differs from the canonical sequence as follows:
     317-321: SDGWA → RDSRN
     322-1199: Missing.

Show »
Length:321
Mass (Da):35,852
Checksum:i9DC2B123A5211A2C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei317 – 3215SDGWA → RDSRN in isoform 3. 1 PublicationVSP_007184
Alternative sequencei322 – 1199878Missing in isoform 3. 1 PublicationVSP_007185Add
BLAST
Alternative sequencei887 – 90620NSNGK…QAPKG → KKRQPEFSPSSQCPSAHVQL in isoform 2. 2 PublicationsVSP_007186Add
BLAST
Alternative sequencei907 – 1199293Missing in isoform 2. 2 PublicationsVSP_007187Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320126 mRNA. Translation: AAG41991.2.
BC067057 mRNA. Translation: AAH67057.1.
BC079566 mRNA. Translation: AAH79566.1.
U89891 mRNA. Translation: AAB48099.1.
CCDSiCCDS23696.1. [P97772-1]
CCDS48499.1. [P97772-2]
RefSeqiNP_001107805.1. NM_001114333.2. [P97772-2]
NP_058672.1. NM_016976.3. [P97772-1]
XP_006512612.1. XM_006512549.2. [P97772-1]
XP_006512615.1. XM_006512552.2. [P97772-2]
UniGeneiMm.391904.
Mm.482986.

Genome annotation databases

EnsembliENSMUST00000044306; ENSMUSP00000037255; ENSMUSG00000019828. [P97772-1]
ENSMUST00000105560; ENSMUSP00000101189; ENSMUSG00000019828. [P97772-2]
ENSMUST00000105561; ENSMUSP00000101190; ENSMUSG00000019828. [P97772-2]
GeneIDi14816.
KEGGimmu:14816.
UCSCiuc007ejh.2. mouse. [P97772-2]
uc007eji.2. mouse. [P97772-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320126 mRNA. Translation: AAG41991.2.
BC067057 mRNA. Translation: AAH67057.1.
BC079566 mRNA. Translation: AAH79566.1.
U89891 mRNA. Translation: AAB48099.1.
CCDSiCCDS23696.1. [P97772-1]
CCDS48499.1. [P97772-2]
RefSeqiNP_001107805.1. NM_001114333.2. [P97772-2]
NP_058672.1. NM_016976.3. [P97772-1]
XP_006512612.1. XM_006512549.2. [P97772-1]
XP_006512615.1. XM_006512552.2. [P97772-2]
UniGeneiMm.391904.
Mm.482986.

3D structure databases

ProteinModelPortaliP97772.
SMRiP97772. Positions 35-578, 581-846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200073. 8 interactions.
IntActiP97772. 4 interactions.
MINTiMINT-4595385.

Chemistry

BindingDBiP97772.
ChEMBLiCHEMBL2892.
GuidetoPHARMACOLOGYi289.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP97772.

Proteomic databases

PaxDbiP97772.
PRIDEiP97772.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044306; ENSMUSP00000037255; ENSMUSG00000019828. [P97772-1]
ENSMUST00000105560; ENSMUSP00000101189; ENSMUSG00000019828. [P97772-2]
ENSMUST00000105561; ENSMUSP00000101190; ENSMUSG00000019828. [P97772-2]
GeneIDi14816.
KEGGimmu:14816.
UCSCiuc007ejh.2. mouse. [P97772-2]
uc007eji.2. mouse. [P97772-1]

Organism-specific databases

CTDi2911.
MGIiMGI:1351338. Grm1.

Phylogenomic databases

eggNOGiNOG295200.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218636.
HOVERGENiHBG107965.
InParanoidiP97772.
KOiK04603.
OMAiKTNETAC.
OrthoDBiEOG7Z0JXG.
PhylomeDBiP97772.
TreeFamiTF313240.

Enzyme and pathway databases

ReactomeiREACT_336829. Class C/3 (Metabotropic glutamate/pheromone receptors).
REACT_347934. G alpha (q) signalling events.

Miscellaneous databases

NextBioi287019.
PROiP97772.
SOURCEiSearch...

Gene expression databases

BgeeiP97772.
CleanExiMM_GRM1.
ExpressionAtlasiP97772. baseline and differential.
GenevestigatoriP97772.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001256. GPCR_3_mtglu_rcpt_1.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01051. MTABOTROPC1R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of novel splice variants of mouse mGluR1."
    Zhu H., Ryan K., Chen S.
    Brain Res. Mol. Brain Res. 73:93-103(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: ICR.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  3. Watanabe M.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 945-1127 (ISOFORM 1).
    Strain: C57BL/6J.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiGRM1_MOUSE
AccessioniPrimary (citable) accession number: P97772
Secondary accession number(s): Q6AXG4, Q9EPV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 11, 2003
Last modified: April 1, 2015
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.