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P97772 (GRM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metabotropic glutamate receptor 1

Short name=mGluR1
Gene names
Name:Grm1
Synonyms:Gprc1a, Mglur1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1199 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum (By. similarity).

Subunit structure

Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled glutamate receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

activation of MAPK activity

Inferred from direct assay PubMed 11356865. Source: UniProtKB

activation of MAPKK activity

Inferred from direct assay PubMed 11356865. Source: UniProtKB

cellular response to electrical stimulus

Inferred from mutant phenotype PubMed 21052544. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 10846166. Source: UniProtKB

phospholipase C-activating G-protein coupled glutamate receptor signaling pathway

Traceable author statement PubMed 9185557. Source: GOC

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of MAPK cascade

Inferred from direct assay PubMed 11356865. Source: UniProtKB

regulation of sensory perception of pain

Inferred from direct assay PubMed 11356865. Source: UniProtKB

regulation of synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

sensory perception of pain

Inferred from mutant phenotype PubMed 11356865. Source: UniProtKB

   Cellular_componentG-protein coupled receptor homodimeric complex

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from direct assay PubMed 10846166. Source: UniProtKB

integral component of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

neuron projection

Inferred from direct assay PubMed 21795692. Source: BHF-UCL

neuronal cell body

Traceable author statement PubMed 11356865. Source: UniProtKB

nucleus

Inferred from direct assay PubMed 15579147. Source: MGI

postsynaptic density

Inferred from direct assay PubMed 15579147. Source: MGI

postsynaptic membrane

Traceable author statement PubMed 10936169. Source: UniProtKB

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

PLC activating G-protein coupled glutamate receptor activity

Traceable author statement PubMed 9185557. Source: MGI

glutamate receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein binding

Inferred from physical interaction PubMed 15579147PubMed 18480293PubMed 9808458. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97772-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97772-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     887-906: NSNGKSVSWSEPGGRQAPKG → KKRQPEFSPSSQCPSAHVQL
     907-1199: Missing.
Isoform 3 (identifier: P97772-3)

Also known as: E55;

The sequence of this isoform differs from the canonical sequence as follows:
     317-321: SDGWA → RDSRN
     322-1199: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 11991179Metabotropic glutamate receptor 1
PRO_0000012923

Regions

Topological domain21 – 593573Extracellular Potential
Transmembrane594 – 61421Helical; Name=1; Potential
Topological domain615 – 62915Cytoplasmic Potential
Transmembrane630 – 65021Helical; Name=2; Potential
Topological domain651 – 66010Extracellular Potential
Transmembrane661 – 68121Helical; Name=3; Potential
Topological domain682 – 70625Cytoplasmic Potential
Transmembrane707 – 72721Helical; Name=4; Potential
Topological domain728 – 75124Extracellular Potential
Transmembrane752 – 77221Helical; Name=5; Potential
Topological domain773 – 78614Cytoplasmic Potential
Transmembrane787 – 80721Helical; Name=6; Potential
Topological domain808 – 8114Extracellular Potential
Transmembrane812 – 83221Helical; Name=7; Potential
Topological domain833 – 1199367Cytoplasmic Potential
Region186 – 1883Glutamate binding By similarity
Compositional bias1014 – 104128Gln/Pro-rich
Compositional bias1073 – 108715Gln/Pro-rich
Compositional bias1101 – 113535Asp/Glu-rich (acidic)
Compositional bias1147 – 119953Ser-rich

Sites

Binding site741Glutamate By similarity
Binding site1651Glutamate By similarity
Binding site2361Glutamate By similarity
Binding site3181Glutamate By similarity
Binding site4091Glutamate By similarity

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation3971N-linked (GlcNAc...) Potential
Glycosylation5151N-linked (GlcNAc...) Potential
Glycosylation7471N-linked (GlcNAc...) Potential
Disulfide bond67 ↔ 109 By similarity
Disulfide bond140Interchain By similarity
Disulfide bond289 ↔ 291 By similarity
Disulfide bond378 ↔ 394 By similarity
Disulfide bond432 ↔ 439 By similarity

Natural variations

Alternative sequence317 – 3215SDGWA → RDSRN in isoform 3.
VSP_007184
Alternative sequence322 – 1199878Missing in isoform 3.
VSP_007185
Alternative sequence887 – 90620NSNGK…QAPKG → KKRQPEFSPSSQCPSAHVQL in isoform 2.
VSP_007186
Alternative sequence907 – 1199293Missing in isoform 2.
VSP_007187

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) [UniParc].

Last modified April 11, 2003. Version 2.
Checksum: FE5370AF160CC16E

FASTA1,199133,212
        10         20         30         40         50         60 
MVRLLLIFFP MIFLEMSILP RMPDRKVLLA GASSQRSVAR MDGDVIIGAL FSVHHQPPAE 

        70         80         90        100        110        120 
KVPERKCGEI REQYGIQRVE AMFHTLDKIN ADPVLLPNIT LGSEIRDSCW HSSVALEQSI 

       130        140        150        160        170        180 
EFIRDSLISI RDEKDGLNRC LPDGQTLPPG RTKKPIAGVI GPGSSSVAIQ VQNLLQLFDI 

       190        200        210        220        230        240 
PQIAYSATSI DLSDKTLYKY FLRVVPSDTL QARAMLDIVK RYNWTYVSAV HTEGNYGESG 

       250        260        270        280        290        300 
MDAFKELAAQ EGLCIAHSDK IYSNAGEKSF DRLLRKLRER LPKARVVVCF CEGMTVRGLL 

       310        320        330        340        350        360 
SAMRRLGVVG EFSLIGSDGW ADRDEVIEGY EVEANGGITI KLQSPEVRSF DDYFLKLRLD 

       370        380        390        400        410        420 
TNTRNPWFPE FWQHRFQCRL PGHLLENPNF KKVCTGNESL EENYVQDSKM GFVINAIYAM 

       430        440        450        460        470        480 
AHGLQNMHHA LCPGYVGLCD AMKPIDGRKL LDFLIKSSFV GVSGEEVWFD EKGDAPGRYD 

       490        500        510        520        530        540 
IMNLQYTEAN RYDYVHVGTW HEGVLNIDDY KIQMNKSGMV RSVCSEPCLK GQIKVIRKGE 

       550        560        570        580        590        600 
VSCCWICTAC KENEFVQDEF TCRACDLGWW PNAELTGCEP ITIRYLEWSD IESIIAIAFS 

       610        620        630        640        650        660 
CLGILVTLFV TLIFVLYRDT PVVKSSSREL CYIILAGIFL GYVCPFTLIA KPTTTSCYLQ 

       670        680        690        700        710        720 
RLLVGLSSAM CYSALVTKTN RIARILAGSK KKICTRKPRF MSAWAQVIIA SILISVQLTL 

       730        740        750        760        770        780 
VVTLIIMEPP MPILSYPSIK EVYLICNTSN LGVVAPVGYN GLLIMSCTYY AFKTRNVPAN 

       790        800        810        820        830        840 
FNEAKYIAFT MYTTCIIWLA FVPIYFGSNY KIITTCFAVS LSVTVALGCM FTPKMYIIIA 

       850        860        870        880        890        900 
KPERNVRSAF TTSDVVRMHV GDGKLPCRSN TFLNIFRRKK PGAGNANSNG KSVSWSEPGG 

       910        920        930        940        950        960 
RQAPKGQHVW QRLSVHVKTN ETACNQTAVI KPLTKSYQGS GKSLTFSDAS TKTLYNVEEE 

       970        980        990       1000       1010       1020 
DNTPSTHFSP PSSPSMVVHR RGPPVATTPP LPPHLSAEET PLFLADSVIP KGLPPPLPQQ 

      1030       1040       1050       1060       1070       1080 
QQQPPPQPPP QQPKSLMDQL QGVVTNFGSG IPDFHAVLAG PGTPGNGLRS LYPPPPPPQH 

      1090       1100       1110       1120       1130       1140 
LQMLPLQLST FREEPISPPG EDDDDDSSER FKLLQEFVYE REGNTEEDDL EEEEDLPAAS 

      1150       1160       1170       1180       1190 
KLTPEDSPAL TPPSPFRDSV ASGSSVPSSP VSESVLCTPP NVTYASVILR DYKQSSSTL 

« Hide

Isoform 2 (B) [UniParc].

Checksum: B9223F00A9C6851C
Show »

FASTA906101,710
Isoform 3 (E55) [UniParc].

Checksum: 9DC2B123A5211A2C
Show »

FASTA32135,852

References

« Hide 'large scale' references
[1]"Cloning of novel splice variants of mouse mGluR1."
Zhu H., Ryan K., Chen S.
Brain Res. Mol. Brain Res. 73:93-103(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
Strain: ICR.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[3]Watanabe M.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 945-1127 (ISOFORM 1).
Strain: C57BL/6J.
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF320126 mRNA. Translation: AAG41991.2.
BC067057 mRNA. Translation: AAH67057.1.
BC079566 mRNA. Translation: AAH79566.1.
U89891 mRNA. Translation: AAB48099.1.
CCDSCCDS23696.1. [P97772-1]
CCDS48499.1. [P97772-2]
RefSeqNP_001107805.1. NM_001114333.2. [P97772-2]
NP_058672.1. NM_016976.3. [P97772-1]
XP_006512611.1. XM_006512548.1. [P97772-1]
XP_006512612.1. XM_006512549.1. [P97772-1]
XP_006512615.1. XM_006512552.1. [P97772-2]
UniGeneMm.391904.
Mm.482986.

3D structure databases

ProteinModelPortalP97772.
SMRP97772. Positions 35-578, 581-846.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200073. 9 interactions.
IntActP97772. 4 interactions.
MINTMINT-4595385.

Chemistry

BindingDBP97772.
ChEMBLCHEMBL2892.
GuidetoPHARMACOLOGY289.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP97772.

Proteomic databases

PaxDbP97772.
PRIDEP97772.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044306; ENSMUSP00000037255; ENSMUSG00000019828. [P97772-1]
ENSMUST00000105560; ENSMUSP00000101189; ENSMUSG00000019828. [P97772-2]
ENSMUST00000105561; ENSMUSP00000101190; ENSMUSG00000019828. [P97772-2]
GeneID14816.
KEGGmmu:14816.
UCSCuc007ejh.2. mouse. [P97772-2]
uc007eji.2. mouse. [P97772-1]

Organism-specific databases

CTD2911.
MGIMGI:1351338. Grm1.

Phylogenomic databases

eggNOGNOG295200.
GeneTreeENSGT00750000117484.
HOGENOMHOG000218636.
HOVERGENHBG107965.
InParanoidP97772.
KOK04603.
OMAKTNETAC.
OrthoDBEOG7Z0JXG.
PhylomeDBP97772.
TreeFamTF313240.

Gene expression databases

BgeeP97772.
CleanExMM_GRM1.
GenevestigatorP97772.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001256. GPCR_3_mtglu_rcpt_1.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01051. MTABOTROPC1R.
PR00593. MTABOTROPICR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287019.
PROP97772.
SOURCESearch...

Entry information

Entry nameGRM1_MOUSE
AccessionPrimary (citable) accession number: P97772
Secondary accession number(s): Q6AXG4, Q9EPV6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 11, 2003
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries