P97769 (CITE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cbp/p300-interacting transactivator 1

Alternative name(s):
Melanocyte-specific protein 1

Gene names

Name:	Cited1
Synonyms:	Msg1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner. Ref.3 Ref.5 Ref.7
Subunit structure	Homodimer. Binds to RBM14. Interacts (via N-terminus) with HSPA8; the interaction suppresses the association of CITED1 with p300/CBP and SMAD-mediated transcription transactivation. Interacts (via C-terminus) with TOX3 (via HGM box); the interaction increases estrogen-response element (ERE)-dependent transcription and protection against cell death. Interacts with ESR1; the interaction occurs in a estrogen-dependent manner By similarity. Interacts (unphosphorylated form preferentially and via C-terminus) with EP300. Interacts (via C-terminus) with CREBBP. Interacts with EGR2. Ref.3 Ref.6
Subcellular location	Nucleus. Cytoplasm By similarity. Note: Shuttles between the nucleus and the cytoplasm by a nuclear export signal (NES) and in a CRM1-dependent manner By similarity. Ref.4 Ref.5 Ref.6
Tissue specificity	Expressed in calvarial osteoblasts. Expressed in nulliparous mammary epithelial cells; absent in pregnant mice and in lacting mammary glands. Also expressed in mammary tumors (at protein level). Expressed only in melanocytes and testis. Expressed at high levels in the strongly pigmented melanoma cells but at low levels in the weakly pigmented cells. Ref.4 Ref.6 Ref.7
Developmental stage	Expressed in trophectoderm-derived cells of the placenta. Ref.5
Induction	Up-regulated by parathyroid hormone, forskolin and phorbol ester in osteoblasts. Ref.7
Post-translational modification	Phosphorylated. Phosphorylation changes in a cell cycle-dependent manner and reduces its transcriptional cofactor activity By similarity.
Disruption phenotype	Mice die shortly after birth. Mice show an abnormal placental development; the spongiotrophoblast layer is irregular in shape and enlarged while the labyrinthine layer is reduced in size, the blood spaces within the labyrinthine are disrupted. Produces more mineralized bone nodules and deposited twice as much calcium in the matrix. Ref.5 Ref.7
Sequence similarities	Belongs to the CITED family.

Ontologies

Keywords
Biological process	Apoptosis Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Activator Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SMAD protein signal transduction Inferred from electronic annotation. Source: Compara apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW brain development Inferred from expression pattern PubMed 17047318. Source: UniProtKB embryonic axis specification Inferred from electronic annotation. Source: Compara labyrinthine layer development Inferred from mutant phenotype Ref.5. Source: MGI melanin biosynthetic process Inferred from direct assay PubMed 11434569. Source: UniProtKB melanocyte differentiation Inferred from mutant phenotype Ref.1. Source: UniProtKB negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of osteoblast differentiation Inferred from mutant phenotype Ref.7. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB nucleocytoplasmic transport Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from direct assay Ref.6PubMed 9811838. Source: UniProtKB response to cAMP Inferred from direct assay Ref.7. Source: UniProtKB response to estrogen stimulus Inferred from electronic annotation. Source: Compara response to insulin stimulus Inferred from direct assay PubMed 9811838. Source: UniProtKB response to interferon-gamma Inferred from direct assay PubMed 9811838. Source: UniProtKB response to interleukin-1 Inferred from direct assay PubMed 9811838. Source: UniProtKB response to interleukin-11 Inferred from direct assay PubMed 9811838. Source: UniProtKB response to interleukin-2 Inferred from direct assay PubMed 9811838. Source: UniProtKB response to interleukin-4 Inferred from direct assay PubMed 9811838. Source: UniProtKB response to interleukin-6 Inferred from direct assay PubMed 9811838. Source: UniProtKB response to interleukin-9 Inferred from direct assay PubMed 9811838. Source: UniProtKB response to lipopolysaccharide Inferred from direct assay PubMed 9811838. Source: UniProtKB response to parathyroid hormone stimulus Inferred from direct assay Ref.7. Source: UniProtKB response to transforming growth factor beta stimulus Inferred from sequence or structural similarity. Source: UniProtKB spongiotrophoblast layer development Inferred from mutant phenotype Ref.5. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara vasculogenesis Inferred from mutant phenotype Ref.5. Source: MGI
Cellular_component	cytosol Inferred from direct assay Ref.4. Source: UniProtKB nucleus Inferred from direct assay Ref.4Ref.5PubMed 17047318Ref.6Ref.1PubMed 9811838. Source: UniProtKB
Molecular_function	chromatin binding Inferred from direct assay Ref.6. Source: UniProtKB protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding transcription factor activity Non-traceable author statement Ref.1. Source: UniProtKB transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB transcription regulatory region DNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Cbp/p300-interacting transactivator 1		PRO_0000144725
Regions
	Motif	168 – 177	10	Nuclear export signal By similarity
	Compositional bias	159 – 198	40	Asp/Glu-rich (acidic)

Sequences

	Sequence		Length	Mass (Da)
	P97769 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: BE968A5182873003	FASTA	203	20,800
	10 20 30 40 50 60 MPTMSRPALD VKGGTTSGKE DANQEMNSLA YSNLGVKDRK AVTVLHYPGV TANGAKANGV 70 80 90 100 110 120 PTSSSGSTSP IGSPTATPSS KPPSFNLHPT PHLMASMQLQ KLNSQYQGAA ATAAAALTGA 130 140 150 160 170 180 GLPGEEEPMQ NWVTAPLVVG GSPGSVSPPA GAQSPALIDS DPVDEEVLMS LVVELGLDRA 190 200 NELPELWLGQ NEFDFTADFP SGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation." Shioda T., Fenner M.H., Isselbacher K.J. Proc. Natl. Acad. Sci. U.S.A. 93:12298-12303(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"The MSG1 non-DNA-binding transactivator binds to the p300/CBP coactivators, enhancing their functional link to the Smad transcription factors." Yahata T., de Caestecker M.P., Lechleider R.J., Andriole S., Roberts A.B., Isselbacher K.J., Shioda T. J. Biol. Chem. 275:8825-8834(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CREBBP AND EP300.
[4]	"Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein." Yahata T., Shao W., Endoh H., Hur J., Coser K.R., Sun H., Ueda Y., Kato S., Isselbacher K.J., Brown M., Shioda T. Genes Dev. 15:2598-2612(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Cited1 is required in trophoblasts for placental development and for embryo growth and survival." Rodriguez T.A., Sparrow D.B., Scott A.N., Withington S.L., Preis J.I., Michalicek J., Clements M., Tsang T.E., Shioda T., Beddington R.S., Dunwoodie S.L. Mol. Cell. Biol. 24:228-244(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[6]	"An EGR2/CITED1 transcription factor complex and the 14-3-3sigma tumor suppressor are involved in regulating ErbB2 expression in a transgenic-mouse model of human breast cancer." Dillon R.L., Brown S.T., Ling C., Shioda T., Muller W.J. Mol. Cell. Biol. 27:8648-8657(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EGR2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"CBP/p300-interacting protein CITED1 modulates parathyroid hormone regulation of osteoblastic differentiation." Yang D., Guo J., Divieti P., Shioda T., Bringhurst F.R. Endocrinology 149:1728-1735(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U65091 mRNA. Translation: AAC53048.1. BC052030 mRNA. Translation: AAH52030.1.
IPI	IPI00129049.
PIR	JC6113.
RefSeq	NP_001263395.1. NM_001276466.1. NP_001263402.1. NM_001276473.1. NP_031735.1. NM_007709.4.
UniGene	Mm.2390.
3D structure databases
ProteinModelPortal	P97769.
SMR	P97769. Positions 160-199.
ModBase	Search...
PTM databases
PhosphoSite	P97769.
Proteomic databases
PRIDE	P97769.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050551; ENSMUSP00000051789; ENSMUSG00000051159. ENSMUST00000101336; ENSMUSP00000098890; ENSMUSG00000051159.
GeneID	12705.
KEGG	mmu:12705.
Organism-specific databases
CTD	4435.
MGI	MGI:108023. Cited1.
Phylogenomic databases
eggNOG	NOG41708.
HOGENOM	HOG000072675.
HOVERGEN	HBG075182.
InParanoid	P97769.
OrthoDB	EOG4X97JC.
Gene expression databases
ArrayExpress	P97769.
Bgee	P97769.
Genevestigator	P97769.
GermOnline	ENSMUSG00000051159. Mus musculus.
Family and domain databases
InterPro	IPR007576. CITED. [Graphical view]
PANTHER	PTHR17045. PTHR17045. 1 hit.
Pfam	PF04487. CITED. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CITED1. mouse.
NextBio	281970.
SOURCE	Search...

Entry information

Entry name

CITE1_MOUSE

Accession

Primary (citable) accession number: P97769

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program