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P97769 (CITE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cbp/p300-interacting transactivator 1
Alternative name(s):
Melanocyte-specific protein 1
Gene names
Name:Cited1
Synonyms:Msg1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length203 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner. Ref.3 Ref.5 Ref.7

Subunit structure

Homodimer. Binds to RBM14. Interacts (via N-terminus) with HSPA8; the interaction suppresses the association of CITED1 with p300/CBP and SMAD-mediated transcription transactivation. Interacts (via C-terminus) with TOX3 (via HGM box); the interaction increases estrogen-response element (ERE)-dependent transcription and protection against cell death. Interacts with ESR1; the interaction occurs in a estrogen-dependent manner By similarity. Interacts (unphosphorylated form preferentially and via C-terminus) with EP300. Interacts (via C-terminus) with CREBBP. Interacts with EGR2. Ref.3 Ref.6

Subcellular location

Nucleus. Cytoplasm By similarity. Note: Shuttles between the nucleus and the cytoplasm by a nuclear export signal (NES) and in a CRM1-dependent manner By similarity. Ref.4 Ref.5 Ref.6

Tissue specificity

Expressed in calvarial osteoblasts. Expressed in nulliparous mammary epithelial cells; absent in pregnant mice and in lacting mammary glands. Also expressed in mammary tumors (at protein level). Expressed only in melanocytes and testis. Expressed at high levels in the strongly pigmented melanoma cells but at low levels in the weakly pigmented cells. Ref.4 Ref.6 Ref.7

Developmental stage

Expressed in trophectoderm-derived cells of the placenta. Ref.5

Induction

Up-regulated by parathyroid hormone, forskolin and phorbol ester in osteoblasts. Ref.7

Post-translational modification

Phosphorylated. Phosphorylation changes in a cell cycle-dependent manner and reduces its transcriptional cofactor activity By similarity.

Disruption phenotype

Mice die shortly after birth. Mice show an abnormal placental development; the spongiotrophoblast layer is irregular in shape and enlarged while the labyrinthine layer is reduced in size, the blood spaces within the labyrinthine are disrupted. Produces more mineralized bone nodules and deposited twice as much calcium in the matrix. Ref.5 Ref.7

Sequence similarities

Belongs to the CITED family.

Ontologies

Keywords
   Biological processApoptosis
Differentiation
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Molecular functionActivator
Developmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSMAD protein signal transduction

Inferred from electronic annotation. Source: Ensembl

apoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

brain development

Inferred from expression pattern PubMed 17047318. Source: UniProtKB

embryonic axis specification

Inferred from electronic annotation. Source: Ensembl

labyrinthine layer development

Inferred from mutant phenotype Ref.5. Source: MGI

melanin biosynthetic process

Inferred from direct assay PubMed 11434569. Source: UniProtKB

melanocyte differentiation

Inferred from mutant phenotype Ref.1. Source: UniProtKB

negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis

Inferred from electronic annotation. Source: Ensembl

negative regulation of neuron apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of osteoblast differentiation

Inferred from mutant phenotype Ref.7. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

nucleocytoplasmic transport

Inferred from sequence or structural similarity. Source: UniProtKB

pigmentation

Inferred from direct assay PubMed 11434569. Source: UniProtKB

placenta development

Inferred from mutant phenotype Ref.5. Source: UniProtKB

positive regulation of gene expression

Inferred from direct assay PubMed 11434569. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.6PubMed 9811838. Source: UniProtKB

response to cAMP

Inferred from direct assay Ref.7. Source: UniProtKB

response to cytokine

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to estrogen

Inferred from electronic annotation. Source: Ensembl

response to insulin

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to interferon-gamma

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to interleukin-1

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to interleukin-11

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to interleukin-2

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to interleukin-4

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to interleukin-6

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to interleukin-9

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to lipopolysaccharide

Inferred from direct assay PubMed 9811838. Source: UniProtKB

response to parathyroid hormone

Inferred from direct assay Ref.7. Source: UniProtKB

response to transforming growth factor beta

Inferred from sequence or structural similarity. Source: UniProtKB

spongiotrophoblast layer development

Inferred from mutant phenotype Ref.5. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transforming growth factor beta receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

vasculogenesis

Inferred from mutant phenotype Ref.5. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay Ref.5PubMed 17615577. Source: UniProtKB

cytosol

Inferred from direct assay Ref.4. Source: UniProtKB

nucleus

Inferred from direct assay Ref.4Ref.5PubMed 17047318Ref.6Ref.1PubMed 9811838. Source: UniProtKB

   Molecular_functionchromatin binding

Inferred from direct assay Ref.6. Source: UniProtKB

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Non-traceable author statement Ref.1. Source: UniProtKB

transcription coactivator activity

Inferred from sequence or structural similarity. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 203203Cbp/p300-interacting transactivator 1
PRO_0000144725

Regions

Motif168 – 17710Nuclear export signal By similarity
Compositional bias159 – 19840Asp/Glu-rich (acidic)

Sequences

Sequence LengthMass (Da)Tools
P97769 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: BE968A5182873003

FASTA20320,800
        10         20         30         40         50         60 
MPTMSRPALD VKGGTTSGKE DANQEMNSLA YSNLGVKDRK AVTVLHYPGV TANGAKANGV 

        70         80         90        100        110        120 
PTSSSGSTSP IGSPTATPSS KPPSFNLHPT PHLMASMQLQ KLNSQYQGAA ATAAAALTGA 

       130        140        150        160        170        180 
GLPGEEEPMQ NWVTAPLVVG GSPGSVSPPA GAQSPALIDS DPVDEEVLMS LVVELGLDRA 

       190        200 
NELPELWLGQ NEFDFTADFP SGC 

« Hide

References

« Hide 'large scale' references
[1]"msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation."
Shioda T., Fenner M.H., Isselbacher K.J.
Proc. Natl. Acad. Sci. U.S.A. 93:12298-12303(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"The MSG1 non-DNA-binding transactivator binds to the p300/CBP coactivators, enhancing their functional link to the Smad transcription factors."
Yahata T., de Caestecker M.P., Lechleider R.J., Andriole S., Roberts A.B., Isselbacher K.J., Shioda T.
J. Biol. Chem. 275:8825-8834(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CREBBP AND EP300.
[4]"Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein."
Yahata T., Shao W., Endoh H., Hur J., Coser K.R., Sun H., Ueda Y., Kato S., Isselbacher K.J., Brown M., Shioda T.
Genes Dev. 15:2598-2612(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"Cited1 is required in trophoblasts for placental development and for embryo growth and survival."
Rodriguez T.A., Sparrow D.B., Scott A.N., Withington S.L., Preis J.I., Michalicek J., Clements M., Tsang T.E., Shioda T., Beddington R.S., Dunwoodie S.L.
Mol. Cell. Biol. 24:228-244(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[6]"An EGR2/CITED1 transcription factor complex and the 14-3-3sigma tumor suppressor are involved in regulating ErbB2 expression in a transgenic-mouse model of human breast cancer."
Dillon R.L., Brown S.T., Ling C., Shioda T., Muller W.J.
Mol. Cell. Biol. 27:8648-8657(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EGR2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"CBP/p300-interacting protein CITED1 modulates parathyroid hormone regulation of osteoblastic differentiation."
Yang D., Guo J., Divieti P., Shioda T., Bringhurst F.R.
Endocrinology 149:1728-1735(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U65091 mRNA. Translation: AAC53048.1.
BC052030 mRNA. Translation: AAH52030.1.
PIRJC6113.
RefSeqNP_001263395.1. NM_001276466.1.
NP_001263402.1. NM_001276473.1.
NP_001263403.1. NM_001276474.1.
NP_031735.1. NM_007709.4.
UniGeneMm.2390.

3D structure databases

ProteinModelPortalP97769.
SMRP97769. Positions 160-199.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198721. 1 interaction.

PTM databases

PhosphoSiteP97769.

Proteomic databases

PRIDEP97769.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050551; ENSMUSP00000051789; ENSMUSG00000051159.
ENSMUST00000101336; ENSMUSP00000098890; ENSMUSG00000051159.
GeneID12705.
KEGGmmu:12705.
UCSCuc009tyn.2. mouse.

Organism-specific databases

CTD4435.
MGIMGI:108023. Cited1.

Phylogenomic databases

eggNOGNOG41708.
HOGENOMHOG000072675.
HOVERGENHBG075182.
InParanoidP97769.
OMAQYHGMAA.
OrthoDBEOG7TJ3K5.
PhylomeDBP97769.
TreeFamTF331915.

Gene expression databases

BgeeP97769.
GenevestigatorP97769.

Family and domain databases

InterProIPR007576. CITED.
[Graphical view]
PANTHERPTHR17045. PTHR17045. 1 hit.
PfamPF04487. CITED. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCITED1. mouse.
NextBio281970.
PROP97769.
SOURCESearch...

Entry information

Entry nameCITE1_MOUSE
AccessionPrimary (citable) accession number: P97769
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot