P97766 (CFC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cryptic protein

Gene names

Name:

Cfc1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nodal coreceptor involved in the correct establishment of the left-right axis. May play a role in mesoderm and/or neural patterning during gastrulation. Ref.1 Ref.4
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity. Secreted By similarity.
Tissue specificity	No expressed in adult tissues. Ref.1
Developmental stage	Expressed during gastrulation (from 6.5 dpc to 11 dpc) in two spatial domains that correspond to the axial and lateral mesoderm. In the first domain expression is progressively localized to the anterior primitive streak, the head process, and the node and notochordal. In the second domain, expression is initially concentrated in the lateral region of the egg cylinder, and is later found circumferentially in the intermediate and lateral plate mesoderm. Furthermore, the expression can also be detected at the early head-fold stage in the midline neuroectoderm, and consequently is an early marker for the prospective floor plate of the neural tube. Expression ceases at the end of gastrulation, and has not been observed in later embryonic stages. Ref.1
Post-translational modification	N-glycosylated. Ref.1
Disruption phenotype	Positional defects in internal organs. The lung presents a right pulmonary isomerism. The stomach is located on either the left or the right and the spleen is small and has an abnormal shape. The apex of the heart pointed to the right or left. In addition malpositioning of heart outflow tracts is observed, the aorta is connected to the right ventricle and emerged from the heart in a ventral position and to the right of the pulmonary artery. This one is connected to either the left or the right ventricle. Ref.4
Sequence similarities	Contains 1 EGF-like domain.

Ontologies

Keywords
Biological process	Gastrulation
Cellular component	Cell membrane Membrane Secreted
Domain	EGF-like domain Signal
Molecular function	Developmental protein
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Inferred from direct assay PubMed 17360443. Source: MGI anterior/posterior pattern specification Inferred from genetic interaction PubMed 11412024. Source: MGI atrial septum morphogenesis Inferred from mutant phenotype PubMed 10521397. Source: MGI canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 17360443. Source: MGI digestive tract morphogenesis Inferred from mutant phenotype PubMed 10521397. Source: MGI endoderm development Inferred from genetic interaction PubMed 11412024. Source: MGI gastrulation Inferred from electronic annotation. Source: UniProtKB-KW heart looping Inferred from mutant phenotype PubMed 10521397. Source: MGI left lung morphogenesis Inferred from mutant phenotype PubMed 10521397. Source: MGI liver development Inferred from mutant phenotype PubMed 10521397. Source: MGI nodal signaling pathway Inferred from mutant phenotype PubMed 12052855. Source: UniProtKB post-embryonic development Inferred from mutant phenotype PubMed 10521397. Source: MGI spleen development Inferred from mutant phenotype PubMed 10521397. Source: MGI
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 166	131	Cryptic protein		PRO_0000044631
	Propeptide	167 – 202	36	Removed in mature form By similarity		PRO_0000395409
Regions
	Domain	94 – 123	30	EGF-like
Amino acid modifications
	Lipidation	166	1	GPI-anchor amidated aspartate By similarity
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Disulfide bond	98 ↔ 105		By similarity
	Disulfide bond	99 ↔ 111		By similarity
	Disulfide bond	113 ↔ 122		By similarity
Experimental info
	Sequence conflict	83	1	P → T in AAI00707. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P97766 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 57035AD339A16FD7	FASTA	202	21,792
	10 20 30 40 50 60 MRANSPTQGI SLKMHQARPL FLVTVALQLI GLGYSYQSEG DGAREVSNIL SPVIPGTTLD 70 80 90 100 110 120 RTLSNSSRKN DIPEGARLWD SLPDSSTLGE SAVPVSRCCH NGGTCVLGSF CVCPAYFTGR 130 140 150 160 170 180 YCEHDQRRRD CGALGHGAWT LHSCRLCRCI FSALYCLPHQ TFSHCDLKSF LSSGARGSRE 190 200 CSIPSLLLLV LCLLLQGVAG KG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A differential display strategy identifies Cryptic, a novel EGF-related gene expressed in the axial and lateral mesoderm during mouse gastrulation." Shen M.M., Wang H., Leder P. Development 124:429-442(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, GLYCOSYLATION. Strain: 129/Sv.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Subtractive hybridization identifies chick-cripto, a novel EGF-CFC ortholog expressed during gastrulation, neurulation and early cardiogenesis." Colas J.-F., Schoenwolf G.C. Gene 255:205-217(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
[4]	"A role of the cryptic gene in the correct establishment of the left-right axis." Gaio U., Schweickert A., Fischer A., Garratt A.N., Mueller T., Oezcelik C., Lankes W., Strehle M., Britsch S., Blum M., Birchmeier C. Curr. Biol. 9:1339-1342(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57720 mRNA. Translation: AAC53042.1. BC100705 mRNA. Translation: AAI00706.1. BC100706 mRNA. Translation: AAI00707.1. BC100708 mRNA. Translation: AAI00709.1. BC100711 mRNA. Translation: AAI00712.1. AF242430 Genomic DNA. Translation: AAF76323.1.
IPI	IPI00129046.
RefSeq	NP_031711.1. NM_007685.2.
UniGene	Mm.2531.
3D structure databases
ProteinModelPortal	P97766.
SMR	P97766. Positions 98-166.
ModBase	Search...
Proteomic databases
PRIDE	P97766.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027298; ENSMUSP00000027298; ENSMUSG00000026124.
GeneID	12627.
KEGG	mmu:12627.
UCSC	uc007aow.1. mouse.
Organism-specific databases
CTD	55997.
MGI	MGI:109448. Cfc1.
Phylogenomic databases
eggNOG	NOG311092.
GeneTree	ENSGT00530000063804.
HOGENOM	HOG000090236.
HOVERGEN	HBG099834.
InParanoid	P97766.
OMA	NSSHEEK.
OrthoDB	EOG4CZBHB.
Enzyme and pathway databases
Reactome	REACT_127416. Developmental Biology.
Gene expression databases
Bgee	P97766.
CleanEx	MM_CFC1.
Genevestigator	P97766.
GermOnline	ENSMUSG00000026124. Mus musculus.
Family and domain databases
InterPro	IPR019011. Cryptic/Cripto_CFC-dom. IPR000742. EG-like_dom. IPR013032. EGF-like_CS. [Graphical view]
Pfam	PF09443. CFC. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281804.
SOURCE	Search...

Entry information

Entry name

CFC1_MOUSE

Accession

Primary (citable) accession number: P97766
Secondary accession number(s): Q496U5, Q9JIB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program