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Protein

WW domain-binding protein 2

Gene

Wbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as transcriptional coactivator of estrogen and progesterone receptors (ESR1 and PGR) upon hormone activation. In presence of estrogen, binds to ESR1-responsive promoters. Required for YAP1 coactivation function on PGR activity. Synergizes with WBP2 in enhancing PGR activity (By similarity). Modulates expression of post-synaptic scaffolding proteins via regulation of ESR1, ESR2 and PGR (PubMed:26881968).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
WW domain-binding protein 2
Short name:
WBP-2
Gene namesi
Name:Wbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104709 Wbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant animals have a progressive high-frequency hearing loss (PubMed:26881968). They show swelling of afferent terminals and abnormal expression of AMPA receptor subunit at post-synaptic densities (PubMed:26881968). Mice are fertile and show no other abnormalities (PubMed:26881968).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170Y → A: No effect on interaction with NEDD4. 1 Publication1
Mutagenesisi200Y → A: Abolishes interaction with NEDD4. 1 Publication1
Mutagenesisi252Y → A: No effect on interaction with NEDD4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659511 – 261WW domain-binding protein 2Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192PhosphotyrosineBy similarity1
Modified residuei231PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated in repsonse to EGF as well as estrogen and progesterone hormones. Tyr-192 and Tyr-231 are phosphorylated by YES and SRC inducing nuclear translocation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP97765
PaxDbiP97765
PeptideAtlasiP97765
PRIDEiP97765

PTM databases

iPTMnetiP97765
PhosphoSitePlusiP97765

Expressioni

Tissue specificityi

Expressed in the ear and the eye (PubMed:26881968). Isoform 1 is expressed in brain, inner ear and organ of Corti. Isoform 2 is only detected in brain (PubMed:26881968).1 Publication

Gene expression databases

BgeeiENSMUSG00000034341
CleanExiMM_WBP2
ExpressionAtlasiP97765 baseline and differential
GenevisibleiP97765 MM

Interactioni

Subunit structurei

Binds to the WW domain of YAP1, WWP1 and WWP2 (By similarity) (PubMed:7644498). Interacts with NEDD4 (PubMed:11042109). Interacts with ESR1 and UBE3A (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nedd4P469355EBI-6304181,EBI-773516

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204546, 2 interactors
IntActiP97765, 3 interactors
MINTiP97765
STRINGi10090.ENSMUSP00000074204

Structurei

3D structure databases

ProteinModelPortaliP97765
SMRiP97765
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 84GRAMAdd BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi196 – 200PPxY motif 15
Motifi248 – 252PPxY motif 25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi146 – 255Pro-richAdd BLAST110

Domaini

The PPxY motif 1 mediates interaction with NEDD4 (PubMed:11042109). The PPxY motif 2 is required for the coactivation function (By similarity).By similarity1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3294 Eukaryota
ENOG4111FQW LUCA
GeneTreeiENSGT00530000063718
HOGENOMiHOG000231103
HOVERGENiHBG027489
InParanoidiP97765
OMAiYLMKGCE
OrthoDBiEOG091G0ICX
PhylomeDBiP97765
TreeFamiTF314141

Family and domain databases

InterProiView protein in InterPro
IPR004182 GRAM
IPR033598 WBP2
PANTHERiPTHR31606:SF4 PTHR31606:SF4, 1 hit
PfamiView protein in Pfam
PF02893 GRAM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97765-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALNKNHSEG GGVIVNNTES ILMSYDHVEL TFNDMKNVPE AFKGTKKGTV
60 70 80 90 100
YLTPYRVIFL SKGKDAMQSF MMPFYLMKDC EIKQPVFGAN FIKGIVKAEA
110 120 130 140 150
GGGWEGSASY KLTFTAGGAI EFGQRMLQVA SQASRGEVPN GAYGYPYMPS
160 170 180 190 200
GAYVFPPPVA NGMYPCPPGY PYPPPPPEFY PGPPMMDGAM GYVQPPPPPY
210 220 230 240 250
PGPMEPPVSG PSAPATPAAE AKAAEAAASA YYNPGNPHNV YMPTSQPPPP
260
PYYPPEDKKT Q
Length:261
Mass (Da):28,032
Last modified:May 1, 1997 - v1
Checksum:iDA475B184F284380
GO
Isoform 2 (identifier: P97765-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     133-177: Missing.

Show »
Length:216
Mass (Da):23,309
Checksum:iDA3D16EC2DFC2F79
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_059234133 – 177Missing in isoform 2. Add BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40826 mRNA Translation: AAB40893.1
AL607108 Genomic DNA No translation available.
CH466558 Genomic DNA Translation: EDL34550.1
BC055058 mRNA Translation: AAH55058.1
CCDSiCCDS25657.1 [P97765-1]
CCDS83934.1 [P97765-2]
RefSeqiNP_001334571.1, NM_001347642.1 [P97765-2]
NP_058548.1, NM_016852.2 [P97765-1]
UniGeneiMm.284792
Mm.396971

Genome annotation databases

EnsembliENSMUST00000074628; ENSMUSP00000074204; ENSMUSG00000034341 [P97765-1]
ENSMUST00000106444; ENSMUSP00000102052; ENSMUSG00000034341 [P97765-2]
GeneIDi22378
KEGGimmu:22378
UCSCiuc007mjv.1 mouse [P97765-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiWBP2_MOUSE
AccessioniPrimary (citable) accession number: P97765
Secondary accession number(s): A2A860
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health