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Protein

Endothelin-converting enzyme 1

Gene

ECE1

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Converts big endothelin-1 to endothelin-1.

Catalytic activityi

Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphoramidon.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi591 – 5911Zinc; catalyticPROSITE-ProRule annotation
Active sitei592 – 5921PROSITE-ProRule annotation
Metal bindingi595 – 5951Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi651 – 6511Zinc; catalyticPROSITE-ProRule annotation
Active sitei655 – 6551Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM13.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelin-converting enzyme 1 (EC:3.4.24.71)
Short name:
ECE-1
Gene namesi
Name:ECE1
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252CytoplasmicSequence analysisAdd
BLAST
Transmembranei53 – 7321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini74 – 754681ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Endothelin-converting enzyme 1PRO_0000078219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91PhosphothreonineBy similarity
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence analysis
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence analysis
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence analysis
Glycosylationi346 – 3461N-linked (GlcNAc...)Sequence analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence analysis
Disulfide bondi412 – 412InterchainBy similarity
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence analysis
Glycosylationi616 – 6161N-linked (GlcNAc...)Sequence analysis
Glycosylationi635 – 6351N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP97739.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000010492.

Structurei

3D structure databases

ProteinModelPortaliP97739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiP97739.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR029734. ECE1.
IPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF130. PTHR11733:SF130. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSTYKRATL DEEDLVDSLS EGEVYPNGLQ VNFRNFRSSQ RCWATRTQVE
60 70 80 90 100
KRLIVLVALL AAGLVACLTA LGIQYRTRTP PVCLSEACVS VTSSILNSMN
110 120 130 140 150
PTVDPCQDFF SYACGGWIKA NPVPDGHSRW GAFSNLWEHN QAIIKHLLEN
160 170 180 190 200
STASVSEAER KAQVYYRACM NETRIEELRA KPLMELIEKL GGWNITGPWA
210 220 230 240 250
KDNFQDTLQV VTAHYRTSPF FSVYVSADSK NSNRNVIHVD QSGLGLPSRD
260 270 280 290 300
YYLNKTENEK VLNGYLNYMV QLGKLLGGGD ENAIRAQMQQ ILDFETALAN
310 320 330 340 350
ITIPQEKRRD EELIYHKVTA AELQTLAPAI NWLPFLNTIF YPVEINESEP
360 370 380 390 400
IVVYDKEYLE QVSTLINTTD KCLLNNYMIW NLVRKTSSFL DQRFQDADEK
410 420 430 440 450
FMEVMYGTKK TCLPRWKFCV SDTENNLGFG LGPMFVKATF AEDSKNIASE
460 470 480 490 500
IILEIKKAFE ESLSTLKWMD EDTRKSAKEK ADAIYNMIGY PNFIMDPKEL
510 520 530 540 550
DKVFNDYTAV PDLYFENAMR FFNFSWRVTA EQLRKAPNRD QWSMTPPMVN
560 570 580 590 600
AYYSPTKNEI VFPAGILPAP FYTRSSPKAL NFGGIGVVVG HELTHAFDDQ
610 620 630 640 650
GREYDKDGNL RPWWKNSSVE AFKQQTECMV EQYSNYSVNG EPVNGRHTLG
660 670 680 690 700
ENIADNGGLK AAYRAYQNWV KKNGAEETLP TLGLTNNQLF FLGFAQVWCS
710 720 730 740 750
VRTPESSHEG LITDPHSPSR FRVIGSLSNS KEFSEHFQCP PGSPMNPRHK

CEVW
Length:754
Mass (Da):85,772
Last modified:May 1, 1997 - v1
Checksum:iF0646C57FF2BA8A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82653 mRNA. Translation: AAB46734.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82653 mRNA. Translation: AAB46734.1.

3D structure databases

ProteinModelPortaliP97739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000010492.

Protein family/group databases

MEROPSiM13.002.

Proteomic databases

PRIDEiP97739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiP97739.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR029734. ECE1.
IPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF130. PTHR11733:SF130. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Endothelin-1 production and endothelin converting enzyme expression by guinea pig airway epithelial cells."
    Shima H., Yamanouchi M., Omori K., Sugiura M., Kawashima K., Sato T.
    Biochem. Mol. Biol. Int. 37:1001-1010(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiECE1_CAVPO
AccessioniPrimary (citable) accession number: P97739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: January 20, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.