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P97718 (ADA1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1A adrenergic receptor
Alternative name(s):
Alpha-1A adrenoreceptor
Short name=Alpha-1A adrenoceptor
Alpha-1C adrenergic receptor
Gene names
Name:Adra1a
Synonyms:Adra1c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G11 proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes By similarity.

Subunit structure

Homo- and heterooligomer By similarity. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes By similarity.

Subcellular location

Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein By similarity. Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes By similarity. Ref.6

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.

Sequence caution

The sequence AAB47044.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Nucleus
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of phospholipase C activity

Inferred from electronic annotation. Source: Ensembl

adrenergic receptor signaling pathway

Inferred from direct assay Ref.1. Source: GOC

adult heart development

Inferred from genetic interaction PubMed 12782680. Source: MGI

aging

Inferred from electronic annotation. Source: Ensembl

calcium ion transport into cytosol

Inferred from electronic annotation. Source: Ensembl

cell growth

Inferred from genetic interaction PubMed 12782680. Source: MGI

micturition

Inferred from electronic annotation. Source: Ensembl

negative regulation of Rho protein signal transduction

Inferred from sequence orthology PubMed 18332105. Source: MGI

negative regulation of heart rate involved in baroreceptor response to increased systemic arterial blood pressure

Inferred from mutant phenotype PubMed 12093905. Source: MGI

negative regulation of synaptic transmission, GABAergic

Inferred from electronic annotation. Source: Ensembl

norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 12093905. Source: MGI

organ growth

Inferred from genetic interaction PubMed 12782680. Source: MGI

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 9590558. Source: MGI

pilomotor reflex

Inferred from direct assay PubMed 21335239. Source: BHF-UCL

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of MAPK cascade

Inferred from genetic interaction PubMed 12782680. Source: MGI

positive regulation of action potential

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle contraction

Inferred from electronic annotation. Source: Ensembl

positive regulation of heart rate by epinephrine-norepinephrine

Inferred from genetic interaction PubMed 12782680. Source: MGI

positive regulation of protein kinase C signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle contraction

Inferred from direct assay PubMed 21335239. Source: BHF-UCL

positive regulation of systemic arterial blood pressure

Inferred from electronic annotation. Source: Ensembl

positive regulation of the force of heart contraction by epinephrine-norepinephrine

Inferred from genetic interaction PubMed 12595294. Source: MGI

positive regulation of vasoconstriction

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to hormone

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentT-tubule

Inferred from electronic annotation. Source: Ensembl

Z disc

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from direct assay Ref.1. Source: MGI

nuclear membrane

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionalpha1-adrenergic receptor activity

Inferred from direct assay Ref.1. Source: MGI

protein heterodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Alpha-1A adrenergic receptor
PRO_0000069064

Regions

Topological domain1 – 2727Extracellular By similarity
Transmembrane28 – 5124Helical; Name=1; By similarity
Topological domain52 – 6413Cytoplasmic By similarity
Transmembrane65 – 8824Helical; Name=2; By similarity
Topological domain89 – 9911Extracellular By similarity
Transmembrane100 – 12223Helical; Name=3; By similarity
Topological domain123 – 14321Cytoplasmic By similarity
Transmembrane144 – 16724Helical; Name=4; By similarity
Topological domain168 – 18114Extracellular By similarity
Transmembrane182 – 20524Helical; Name=5; By similarity
Topological domain206 – 27368Cytoplasmic By similarity
Transmembrane274 – 29724Helical; Name=6; By similarity
Topological domain298 – 3058Extracellular By similarity
Transmembrane306 – 32924Helical; Name=7; By similarity
Topological domain330 – 466137Cytoplasmic By similarity
Region334 – 34916Nuclear localization signal By similarity

Amino acid modifications

Modified residue2151Phosphoserine; by PKA Potential
Lipidation3451S-palmitoyl cysteine Potential
Glycosylation71N-linked (GlcNAc...) Potential
Glycosylation131N-linked (GlcNAc...) Potential
Glycosylation221N-linked (GlcNAc...) Potential
Disulfide bond99 ↔ 176 By similarity

Experimental info

Sequence conflict3691G → E in AAC02658. Ref.1
Sequence conflict3951R → C in AAC02658. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97718 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 8C5FE5C67E885795

FASTA46651,744
        10         20         30         40         50         60 
MVLLSENASE GSNCTHPPAQ VNISKAILLG VILGGLIIFG VLGNILVILS VACHRHLHSV 

        70         80         90        100        110        120 
THYYIVNLAV ADLLLTSTVL PFSAIFEILG YWAFGRVFCN IWAAVDVLCC TASIMGLCII 

       130        140        150        160        170        180 
SIDRYIGVSY PLRYPTIVTQ RRGVRALLCV WALSLVISIG PLFGWRQQAP EDETICQINE 

       190        200        210        220        230        240 
EPGYVLFSAL GSFYVPLTII LVMYCRVYVV AKRESRGLKS GLKTDKSDSE QVTLRIHRKN 

       250        260        270        280        290        300 
VPAEGSGVSS AKNKTHFSVR LLKFSREKKA AKTLGIVVGC FVLCWLPFFL VMPIGSFFPN 

       310        320        330        340        350        360 
FKPPETVFKI VFWLGYLNSC INPIIYPCSS QEFKKAFQNV LRIQCLRRRQ SSKHALGYTL 

       370        380        390        400        410        420 
HPPSQAVEGQ HRGMVRIPVG SGETFYKISK TDGVREWKFF SSMPQGSARI TMPKDQSACT 

       430        440        450        460 
TARVRSKSFL QVCCCVGSST PRPEENHQVP TIKIHTISLG ENGEEV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, expression and characterization of cDNA encoding a mouse alpha1a-adrenoceptor."
Xiao L., Scofield M.A., Jeffries W.B.
Br. J. Pharmacol. 124:213-221(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: CD-1.
Tissue: Brain and Kidney.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Spinal cord.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Molecular cloning of alpha 1d-adrenergic receptor and tissue distribution of three alpha 1-adrenergic receptor subtypes in mouse."
Alonso-Llamazares A., Zamanillo D., Casanova E., Ovalle S., Calvo P., Chinchetru M.A.
J. Neurochem. 65:2387-2392(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 197-280.
Tissue: Brain.
[6]"Nuclear localization drives alpha1-adrenergic receptor oligomerization and signaling in cardiac myocytes."
Wright C.D., Wu S.C., Dahl E.F., Sazama A.J., O'Connell T.D.
Cell. Signal. 24:794-802(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF031431 mRNA. Translation: AAC02658.1.
AK079597 mRNA. Translation: BAC37694.1.
CH466535 Genomic DNA. Translation: EDL35992.1.
BC113139 mRNA. Translation: AAI13140.1.
S80220 mRNA. Translation: AAB47044.1. Different initiation.
CCDSCCDS27223.1.
RefSeqNP_001258689.1. NM_001271760.1.
NP_038489.2. NM_013461.4.
XP_006518507.1. XM_006518444.1.
XP_006518508.1. XM_006518445.1.
XP_006518509.1. XM_006518446.1.
XP_006518510.1. XM_006518447.1.
UniGeneMm.57064.

3D structure databases

ProteinModelPortalP97718.
SMRP97718. Positions 29-341.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP97718.
ChEMBLCHEMBL2111452.
GuidetoPHARMACOLOGY22.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP97718.

Proteomic databases

PRIDEP97718.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000054661; ENSMUSP00000053703; ENSMUSG00000045875.
ENSMUST00000159068; ENSMUSP00000124570; ENSMUSG00000045875.
ENSMUST00000161339; ENSMUSP00000125354; ENSMUSG00000045875.
GeneID11549.
KEGGmmu:11549.
UCSCuc007ukh.1. mouse.

Organism-specific databases

CTD148.
MGIMGI:104773. Adra1a.

Phylogenomic databases

eggNOGNOG253615.
GeneTreeENSGT00750000117429.
HOGENOMHOG000239244.
HOVERGENHBG106962.
InParanoidQ8BV77.
KOK04135.
OMACTTARVR.
PhylomeDBP97718.
TreeFamTF331895.

Gene expression databases

ArrayExpressP97718.
BgeeP97718.
CleanExMM_ADRA1A.
GenevestigatorP97718.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002233. ADR_fam.
IPR001004. ADRA1A_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01103. ADRENERGICR.
PR00557. ADRENRGCA1AR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279030.
PROP97718.
SOURCESearch...

Entry information

Entry nameADA1A_MOUSE
AccessionPrimary (citable) accession number: P97718
Secondary accession number(s): O54913, Q8BV77
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries