Reviewed, UniProtKB/Swiss-Prot P97718 (ADA1A_MOUSE)

Names and origin

Protein names

Recommended name:
    Alpha-1A adrenergic receptor
Alternative name(s):
    Alpha 1A-adrenoreceptor
    Alpha 1A-adrenoceptor
    Alpha-1C adrenergic receptor

Gene names

Name:	Adra1a
Synonyms:	Adra1c

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Carboxyl-terminal Ser or Thr residues may be phosphorylated By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger Inferred from direct assay. Source: MGI adult heart development Inferred from genetic interaction. Source: MGI cell growth Inferred from genetic interaction. Source: MGI negative regulation of heart rate in baroreceptor response to increased systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI organ growth Inferred from genetic interaction. Source: MGI positive regulation of MAPKKK cascade Inferred from genetic interaction. Source: MGI positive regulation of heart rate by epinephrine-norepinephrine Inferred from genetic interaction. Source: MGI positive regulation of the force of heart contraction by epinephrine-norepinephrine Inferred from genetic interaction. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Ref.1 Inferred from direct assay. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha1-adrenergic receptor activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Alpha-1A adrenergic receptor		PRO_0000069064
Regions
	Topological domain	1 – 27	27	Extracellular By similarity
	Transmembrane	28 – 51	24	1 By similarity
	Topological domain	52 – 64	13	Cytoplasmic By similarity
	Transmembrane	65 – 88	24	2 By similarity
	Topological domain	89 – 99	11	Extracellular By similarity
	Transmembrane	100 – 122	23	3 By similarity
	Topological domain	123 – 143	21	Cytoplasmic By similarity
	Transmembrane	144 – 167	24	4 By similarity
	Topological domain	168 – 181	14	Extracellular By similarity
	Transmembrane	182 – 205	24	5 By similarity
	Topological domain	206 – 273	68	Cytoplasmic By similarity
	Transmembrane	274 – 297	24	6 By similarity
	Topological domain	298 – 305	8	Extracellular By similarity
	Transmembrane	306 – 329	24	7 By similarity
	Topological domain	330 – 466	137	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	215	1	Phosphoserine; by PKA Potential
	Lipidation	345	1	S-palmitoyl cysteine Potential
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	22	1	N-linked (GlcNAc...) Potential
	Disulfide bond	99 ↔ 176		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97718-1 [UniParc]. Last modified February 11, 2002. Version 2. Checksum: 3649A5A53DBD34DA	FASTA	466	51,763
	10 20 30 40 50 60 MVLLSENASE GSNCTHPPAQ VNISKAILLG VILGGLIIFG VLGNILVILS VACHRHLHSV 70 80 90 100 110 120 THYYIVNLAV ADLLLTSTVL PFSAIFEILG YWAFGRVFCN IWAAVDVLCC TASIMGLCII 130 140 150 160 170 180 SIDRYIGVSY PLRYPTIVTQ RRGVRALLCV WALSLVISIG PLFGWRQQAP EDETICQINE 190 200 210 220 230 240 EPGYVLFSAL GSFYVPLTII LVMYCRVYVV AKRESRGLKS GLKTDKSDSE QVTLRIHRKN 250 260 270 280 290 300 VPAEGSGVSS AKNKTHFSVR LLKFSREKKA AKTLGIVVGC FVLCWLPFFL VMPIGSFFPN 310 320 330 340 350 360 FKPPETVFKI VFWLGYLNSC INPIIYPCSS QEFKKAFQNV LRIQCLRRRQ SSKHALGYTL 370 380 390 400 410 420 HPPSQAVEEQ HRGMVRIPVG SGETFYKISK TDGVCEWKFF SSMPQGSARI TMPKDQSACT 430 440 450 460 TARVRSKSFL QVCCCVGSST PRPEENHQVP TIKIHTISLG ENGEEV

References

[1]	"Molecular cloning, expression and characterization of cDNA encoding a mouse alpha1a-adrenoceptor." Xiao L., Scofield M.A., Jeffries W.B. Br. J. Pharmacol. 124:213-221(1998) [PubMed: 9630362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: CD-1. Tissue: Brain and Kidney.
[2]	"Molecular cloning of alpha 1d-adrenergic receptor and tissue distribution of three alpha 1-adrenergic receptor subtypes in mouse." Alonso-Llamazares A., Zamanillo D., Casanova E., Ovalle S., Calvo P., Chinchetru M.A. J. Neurochem. 65:2387-2392(1995) [PubMed: 7595531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 197-280. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF031431 mRNA. Translation: AAC02658.1. S80220 mRNA. Translation: AAB47044.1. Different initiation.
IPI	IPI00128843.
UniGene	Mm.57064
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P97718.
Proteomic databases
PRIDE	P97718.
Genome annotation databases
Ensembl	ENSMUSG00000045875. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:104773. Adra1a.
Phylogenomic databases
HOGENOM	P97718.
HOVERGEN	P97718.
Gene expression databases
ArrayExpress	P97718.
Bgee	P97718.
CleanEx	MM_ADRA1A.
GermOnline	ENSMUSG00000045875. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR001004. Adrene_rcpt_A1Cs. IPR002233. Adrnrgc_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR19266:SF89. Adrene_rcpt_A1Cs. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01103. ADRENERGICR. PR00557. ADRENRGCA1AR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ADA1A_MOUSE

Accession

Primary (citable) accession number: P97718
Secondary accession number(s): O54913

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 11, 2002
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)