Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P97717 (ADA1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1B adrenergic receptor
Alternative name(s):
Alpha-1B adrenoreceptor
Short name=Alpha-1B adrenoceptor
Gene names
Name:Adra1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G11 proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes By similarity.

Subunit structure

Homo- and heterooligomer By similarity. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes By similarity.

Subcellular location

Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein By similarity. Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes By similarity. Ref.3

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Nucleus
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadrenergic receptor signaling pathway

Inferred from direct assay PubMed 11923452PubMed 7911220. Source: GOC

adult heart development

Inferred from genetic interaction PubMed 12782680. Source: MGI

behavioral response to cocaine

Inferred from mutant phenotype PubMed 11923452. Source: MGI

blood vessel remodeling

Inferred from mutant phenotype PubMed 15231500. Source: MGI

cell growth

Inferred from genetic interaction PubMed 12782680. Source: MGI

glucose homeostasis

Inferred from mutant phenotype PubMed 14581480. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 11222061. Source: MGI

negative regulation of Rho protein signal transduction

Inferred from sequence orthology PubMed 18332105. Source: MGI

negative regulation of glycogen catabolic process

Inferred from mutant phenotype PubMed 14581480. Source: MGI

organ growth

Inferred from genetic interaction PubMed 12782680. Source: MGI

positive regulation of MAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of glycogen catabolic process

Inferred from mutant phenotype PubMed 14581480. Source: MGI

positive regulation of heart rate by epinephrine-norepinephrine

Inferred from genetic interaction PubMed 12782680. Source: MGI

positive regulation of the force of heart contraction by epinephrine-norepinephrine

Inferred from genetic interaction PubMed 12595294. Source: MGI

regulation of cardiac muscle contraction

Inferred from electronic annotation. Source: InterPro

regulation of vasoconstriction

Inferred from electronic annotation. Source: InterPro

response to amphetamine

Inferred from mutant phenotype PubMed 11923452PubMed 12871582. Source: MGI

response to morphine

Inferred from mutant phenotype PubMed 11923452. Source: MGI

vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 9326654. Source: MGI

visual learning

Inferred from mutant phenotype PubMed 11222061. Source: MGI

   Cellular_componentintegral component of membrane

Inferred from direct assay PubMed 11923452. Source: MGI

nuclear membrane

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha1-adrenergic receptor activity

Inferred from direct assay PubMed 11923452PubMed 7911220. Source: MGI

protein heterodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Alpha-1B adrenergic receptor
PRO_0000069071

Regions

Topological domain1 – 4545Extracellular By similarity
Transmembrane46 – 6924Helical; Name=1; By similarity
Topological domain70 – 8213Cytoplasmic By similarity
Transmembrane83 – 10422Helical; Name=2; By similarity
Topological domain105 – 11410Extracellular By similarity
Transmembrane115 – 14026Helical; Name=3; By similarity
Topological domain141 – 16020Cytoplasmic By similarity
Transmembrane161 – 18323Helical; Name=4; By similarity
Topological domain184 – 20017Extracellular By similarity
Transmembrane201 – 22323Helical; Name=5; By similarity
Topological domain224 – 29471Cytoplasmic By similarity
Transmembrane295 – 31824Helical; Name=6; By similarity
Topological domain319 – 3257Extracellular By similarity
Transmembrane326 – 35025Helical; Name=7; By similarity
Topological domain351 – 514164Cytoplasmic By similarity
Region367 – 37711Nuclear localization signal By similarity
Compositional bias370 – 3778Poly-Arg

Amino acid modifications

Lipidation3641S-palmitoyl cysteine Potential
Glycosylation101N-linked (GlcNAc...) Potential
Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation341N-linked (GlcNAc...) Potential
Disulfide bond117 ↔ 194 By similarity

Sequences

Sequence LengthMass (Da)Tools
P97717 [UniParc].

Last modified January 1, 1998. Version 2.
Checksum: 17CFD10123DC2B29

FASTA51456,418
        10         20         30         40         50         60 
MNPDLDTGHN TSAPAHWGEL KDANFTGPNQ TSSNSTLPQL DVTRAISVGC LGAFILFAIV 

        70         80         90        100        110        120 
GNILVILSVA CNRHLRTPTN YFIVNLAIAD LLLSFTDLPF SATLEVLGYW VLGRIFCDIW 

       130        140        150        160        170        180 
AAVDVLCCTA SILSLCAISI DRYIGVRYSL QYPTLVTRRK AILALLSVWV LSTVISIGPL 

       190        200        210        220        230        240 
LGWKEPAPND DKECGVTEEP FYALFSSLGS FYIPLAVILV MYCRVYIVAK RTTKNLEAGV 

       250        260        270        280        290        300 
MKEMSNSKEL TLRIHSKNFH EDTLSSTKAK GHNPRSSIAV KLFKFSREKK AAKTLGIVVG 

       310        320        330        340        350        360 
MFILCWLPFF IALPLGSLFS TLKPPDAVFK VVFWLGYFNS CLNPIIYPCS SKEFKRAFMR 

       370        380        390        400        410        420 
ILGCQCRGGR RRRRRRRLGA CAYTYRPWTR GGSLERSQSR KDSLDDSGSC MSGSQRTLPS 

       430        440        450        460        470        480 
ASPSPGYLGR GTQPPVELCA FPEWKPGALL SLPEPPGRRG RLDSGPLFTF KLLGEPESPG 

       490        500        510 
TEGDASNGGC DTTTDLANGQ PGFKSNMPLA PGHF 

« Hide

References

[1]Cotecchia S., Lattion-Zellweger A.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[2]"Molecular cloning of alpha 1d-adrenergic receptor and tissue distribution of three alpha 1-adrenergic receptor subtypes in mouse."
Alonso-Llamazares A., Zamanillo D., Casanova E., Ovalle S., Calvo P., Chinchetru M.A.
J. Neurochem. 65:2387-2392(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 238-276.
[3]"Nuclear localization drives alpha1-adrenergic receptor oligomerization and signaling in cardiac myocytes."
Wright C.D., Wu S.C., Dahl E.F., Sazama A.J., O'Connell T.D.
Cell. Signal. 24:794-802(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y12738 mRNA. Translation: CAA73272.1.
S80219 mRNA. Translation: AAB47043.1.
UniGeneMm.39086.
Mm.487939.

3D structure databases

ProteinModelPortalP97717.
SMRP97717. Positions 53-363.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP97717.
ChEMBLCHEMBL2111452.
GuidetoPHARMACOLOGY23.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP97717.

Proteomic databases

PaxDbP97717.
PRIDEP97717.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:104774. Adra1b.

Phylogenomic databases

eggNOGNOG253615.
HOVERGENHBG106962.
InParanoidP97717.

Gene expression databases

CleanExMM_ADRA1B.
GenevestigatorP97717.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002233. ADR_fam.
IPR001115. ADRA1B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24248. PTHR24248. 1 hit.
PTHR24248:SF17. PTHR24248:SF17. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01103. ADRENERGICR.
PR00556. ADRENRGCA1BR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP97717.
SOURCESearch...

Entry information

Entry nameADA1B_MOUSE
AccessionPrimary (citable) accession number: P97717
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries