Reviewed, UniProtKB/Swiss-Prot P97717 (ADA1B_MOUSE)

Names and origin

Protein names

Recommended name:
    Alpha-1B adrenergic receptor
Alternative name(s):
    Alpha 1B-adrenoreceptor
    Alpha 1B-adrenoceptor

Gene names

Name:

Adra1b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW adult heart development Inferred from genetic interaction. Source: MGI behavioral response to cocaine Inferred from mutant phenotype. Source: MGI blood vessel remodeling Inferred from mutant phenotype. Source: MGI cell growth Inferred from genetic interaction. Source: MGI glucose homeostasis Inferred from mutant phenotype. Source: MGI locomotory behavior Inferred from mutant phenotype. Source: MGI negative regulation of glycogen catabolic process Inferred from mutant phenotype. Source: MGI organ growth Inferred from genetic interaction. Source: MGI positive regulation of glycogen catabolic process Inferred from mutant phenotype. Source: MGI positive regulation of heart rate by epinephrine-norepinephrine Inferred from genetic interaction. Source: MGI positive regulation of the force of heart contraction by epinephrine-norepinephrine Inferred from genetic interaction. Source: MGI response to amphetamine Inferred from mutant phenotype. Source: MGI response to morphine Inferred from mutant phenotype. Source: MGI vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI visual learning Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane of membrane fraction Inferred from direct assay. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha1-adrenergic receptor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Alpha-1B adrenergic receptor		PRO_0000069071
Regions
	Topological domain	1 – 45	45	Extracellular By similarity
	Transmembrane	46 – 69	24	1 By similarity
	Topological domain	70 – 82	13	Cytoplasmic By similarity
	Transmembrane	83 – 104	22	2 By similarity
	Topological domain	105 – 114	10	Extracellular By similarity
	Transmembrane	115 – 140	26	3 By similarity
	Topological domain	141 – 160	20	Cytoplasmic By similarity
	Transmembrane	161 – 183	23	4 By similarity
	Topological domain	184 – 200	17	Extracellular By similarity
	Transmembrane	201 – 223	23	5 By similarity
	Topological domain	224 – 294	71	Cytoplasmic By similarity
	Transmembrane	295 – 318	24	6 By similarity
	Topological domain	319 – 325	7	Extracellular By similarity
	Transmembrane	326 – 350	25	7 By similarity
	Topological domain	351 – 514	164	Cytoplasmic By similarity
	Compositional bias	370 – 377	8	Poly-Arg
Amino acid modifications
	Modified residue	263	1	Phosphothreonine Ref.3
	Modified residue	266	1	Phosphoserine Ref.3
	Lipidation	364	1	S-palmitoyl cysteine Potential
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Disulfide bond	117 ↔ 194		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97717-1 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: 17CFD10123DC2B29	FASTA	514	56,418
	10 20 30 40 50 60 MNPDLDTGHN TSAPAHWGEL KDANFTGPNQ TSSNSTLPQL DVTRAISVGC LGAFILFAIV 70 80 90 100 110 120 GNILVILSVA CNRHLRTPTN YFIVNLAIAD LLLSFTDLPF SATLEVLGYW VLGRIFCDIW 130 140 150 160 170 180 AAVDVLCCTA SILSLCAISI DRYIGVRYSL QYPTLVTRRK AILALLSVWV LSTVISIGPL 190 200 210 220 230 240 LGWKEPAPND DKECGVTEEP FYALFSSLGS FYIPLAVILV MYCRVYIVAK RTTKNLEAGV 250 260 270 280 290 300 MKEMSNSKEL TLRIHSKNFH EDTLSSTKAK GHNPRSSIAV KLFKFSREKK AAKTLGIVVG 310 320 330 340 350 360 MFILCWLPFF IALPLGSLFS TLKPPDAVFK VVFWLGYFNS CLNPIIYPCS SKEFKRAFMR 370 380 390 400 410 420 ILGCQCRGGR RRRRRRRLGA CAYTYRPWTR GGSLERSQSR KDSLDDSGSC MSGSQRTLPS 430 440 450 460 470 480 ASPSPGYLGR GTQPPVELCA FPEWKPGALL SLPEPPGRRG RLDSGPLFTF KLLGEPESPG 490 500 510 TEGDASNGGC DTTTDLANGQ PGFKSNMPLA PGHF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Cotecchia S., Lattion-Zellweger A. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain.
[2]	"Molecular cloning of alpha 1d-adrenergic receptor and tissue distribution of three alpha 1-adrenergic receptor subtypes in mouse." Alonso-Llamazares A., Zamanillo D., Casanova E., Ovalle S., Calvo P., Chinchetru M.A. J. Neurochem. 65:2387-2392(1995) [PubMed: 7595531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 238-276.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-263 AND SER-266, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y12738 mRNA. Translation: CAA73272.1. S80219 mRNA. Translation: AAB47043.1.
IPI	IPI00314429.
UniGene	Mm.39086
3D structure databases
HSSP	HSSP built from PDB template 1MMH based on UniProtKB P29274.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P97717.
Genome annotation databases
Ensembl	ENSMUSG00000050541. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:104774. Adra1b.
Phylogenomic databases
HOVERGEN	P97717.
Gene expression databases
ArrayExpress	P97717.
Bgee	P97717.
CleanEx	MM_ADRA1B.
GermOnline	ENSMUSG00000050541. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR001115. Adrene_rcpt_A1B. IPR002233. Adrnrgc_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01103. ADRENERGICR. PR00556. ADRENRGCA1BR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

ADA1B_MOUSE

Accession

Primary (citable) accession number: P97717

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)